| 载体名称: | pCMV-Gluc |
|---|---|
| 载体又名: | pCMV-Gluc载体, pCMV-Gluc载体质粒，pCMV-Gluc质粒，pCMV-Gluc-1载体, pCMV-Gluc-1载体质粒，pCMV-Gluc-1质粒 |
| 英文名称: | pCMV-Gluc, pCMV-Gluc Vector, pCMV-Gluc Plasmid, pCMV-Gluc-1, pCMV-Gluc-1 Vector, pCMV-Gluc-1 Plasmid |
| 出品公司: | Nanolight |
| 质粒类型: | 哺乳动物细胞表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | CMV |
| 载体大小: | 5764 bp |
| 5’测序引物及序列: | CMV-F: CGCAAATGGGCGGTAGGCGTG |
| 3’测序引物及序列: | pBABE 3': ACCCTAACTGACACACATTCC |
| 载体标签: | C-Gluc |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 新霉素Neomycin |
| 克隆菌株: | DH5α、TOP10等 |
| 宿主细胞（系）: | 常规细胞系（293、CHO等） |
| 备注: | 用于荧光素酶检测。 |
| 产品目录号: | 202 |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
| 相关文件: |
pCMV-Gluc载体序列PDF版 pCMV-Gluc载体序列Snapgene格式 |
买家导航
pCMVGluc pCMV-Gluc-1载体基本信息
pCMVGluc pCMV-Gluc-1载体图谱质粒图谱和多克隆位点信息
pCMVGluc pCMV-Gluc-1载体简介
pCMV-Gluc载体，含有受 CMV（巨细胞病毒）启动子控制的 Gaussia 荧光素酶基因。此组成型表达载体可用作归一化对照品，以结合其他报告基因解释实验变化。
pCMV-Gluc载体含有哺乳动物（人）密码子优化的Gaussia荧光素酶，表达N-末端分泌信号。该产品可用作阳性对照载体，在使用Gaussia荧光素酶作为报告载体，评估基因表达效率方面非常有用。该载体同时具有氨苄西林抗性和新霉素抗性。因此，它很容易在大肠杆菌中繁殖，并可用于建立稳定的表达Gaussia荧光素酶的细胞系。
另外，有研究使用Gaussia荧光素酶与CRISPR一起用作报告质粒，以研究非编码RNA（ncRNA）的功能：Multiplexable, locus-specific targeting of long RNAs with CRISPR-Display. Shechner DM, Hacisuleyman E, Younger ST, Rinn JL. July 2015. PubMed
pCMV-GLuc 载体的特点：
•用于共转染和归一化的强 (CMV) 组成型启动子
•天然分泌Gaussia 荧光素酶基因，经优化可实现哺乳动物系统中的高表达
•多克隆位点 (MCS) 为调控元件从一个质粒至另一个质粒的转移提供了多功能性
•用于通过降低转录的通读来最小化背景的转录终止位点 (Ter)、Lac 操纵基因 (Lac O1) 和转录中断位点 (TPS)
• 高拷贝 pUC 细菌 DNA 复制起点
Gaussia Luc 载体含有从桡足动物 Gaussia princeps 中克隆的基因。该基因可编码一个自然分泌的生物发光 Gaussia 荧光素酶（约 20 kDa），可测量培养基（实时检测）和细胞裂解物中的报告基因活性。
pCMVGluc pCMV-Gluc-1载体序列
LOCUS pCMV-Gluc 5764 bp ds-DNA circular SYN 13-MAR-2023
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5764)
AUTHORS biofeng.com
TITLE Direct Submission
JOURNAL Exported Monday, Mar 13, 2023 from SnapGene 4.3.6 to Vector NTI(R)
format
https://www.biofeng.com
COMMENT VNTDATE|1015804800|
COMMENT VNTDBDATE|1015804800|
COMMENT VNTNAME|pCMV-Gluc.dna|
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 863..881
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
regulatory 914..923
/regulatory_class="other"
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 920..1477
/codon_start=1
/product="secreted Gaussia luciferase"
/label=hGLuc
/note="human codon-optimized"
rep_origin 1630..2058
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2072..2402
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 2253..2388
/label=SV40 ori
/note="SV40 origin of replication"
CDS 2469..3263
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
polyA_signal 3437..3558
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3607..3623)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 3631..3647
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(3655..3685)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 3700..3721
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(4009..4597)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4768..5628)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
promoter complement(5629..5733)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
901 gagctcggat ccagccacca tgggagtcaa agttctgttt gccctgatct gcatcgctgt
961 ggccgaggcc aagcccaccg agaacaacga agacttcaac atcgtggccg tggccagcaa
1021 cttcgcgacc acggatctcg atgctgaccg cgggaagttg cccggcaaga agctgccgct
1081 ggaggtgctc aaagagatgg aagccaatgc ccggaaagct ggctgcacca ggggctgtct
1141 gatctgcctg tcccacatca agtgcacgcc caagatgaag aagttcatcc caggacgctg
1201 ccacacctac gaaggcgaca aagagtccgc acagggcggc ataggcgagg cgatcgtcga
1261 cattcctgag attcctgggt tcaaggactt ggagcccatg gagcagttca tcgcacaggt
1321 cgatctgtgt gtggactgca caactggctg cctcaaaggg cttgccaacg tgcagtgttc
1381 tgacctgctc aagaagtggc tgccgcaacg ctgtgcgacc tttgccagca agatccaggg
1441 ccaggtggac aagatcaagg gggccggtgg tgactaagcg gccgctcgag catgcatcta
1501 gaaataattc ttactgtcat gccaagtaag atgcttttct gtgctgcaat agcaggcatg
1561 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
1621 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
1681 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
1741 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcggggcatc cctttagggt
1801 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
1861 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
1921 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
1981 ttgatttata agggattttg gggatttcgg cctattggtt aaaaaatgag ctgatttaac
2041 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
2101 aggctcccca ggcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
2161 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
2221 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
2281 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
2341 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
2401 aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat
2461 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
2521 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
2581 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
2641 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
2701 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
2761 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
2821 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
2881 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
2941 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca
3001 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
3061 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
3121 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
3181 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
3241 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
3301 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
3361 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
3421 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag
3481 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
3541 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta
3601 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
3661 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
3721 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
3781 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
3841 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
3901 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
3961 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
4021 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
4081 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
4141 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
4201 tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
4261 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
4321 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
4381 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
4441 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
4501 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
4561 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
4621 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
4681 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
4741 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
4801 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
4861 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
4921 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
4981 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
5041 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
5101 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
5161 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
5221 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
5281 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
5341 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
5401 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
5461 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
5521 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
5581 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
5641 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
5701 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
5761 cgtc
//
pCMVGluc pCMV-Gluc-1载体图谱质粒图谱pdf版和相关资料下载
pCMVGluc pCMV-Gluc-1载体质粒应用举例
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