| 质粒名称: | MXS_BidirectionalCAG |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 78175 |
| 存储实验室: | Pierre Neveu |
|
载体骨架基本信息 |
|
| 载体名称: | 改良MXS链载体Addgene 62394 |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 1930 bp |
| 完整质粒大小: | 6230 bp |
| 载体类型: | 哺乳动物表达，合成生物学 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Bidirectional CAG Promoter |
| 物种来源: | Synthetic |
| 插入大小: | 4280 bp |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | MluI(未破坏) |
| 3' 克隆位点: | SalI (未破坏) |
| 5' 测序引物 | TTACCGCCTTTGAGTGAG |
| 3' 测序引物 | TTGTCTCATGAGCGGATAC |
|
文献引用方法 |
|
| 材料和方法部分: | MXS_BidirectionalCAG was a gift from Pierre Neveu (Addgene plasmid # 78175 ; http://n2t.net/addgene:78175 ; RRID:Addgene_78175) |
| 参考文献部分: | Bidirectional Promoter Engineering for Single Cell MicroRNA Sensors in Embryonic Stem Cells. Sladitschek HL, Neveu PA. PLoS One. 2016 May 6;11(5):e0155177. doi: 10.1371/journal.pone.0155177. eCollection 2016. PONE-D-16-09196 [pii] PubMed 27152616 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS MXS_BidirectionalCAG 6221 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Bidirectional CAG promoter (compatible with the MXS Chaining Kit).
ACCESSION .
VERSION .
KEYWORDS MXS_BidirectionalCAG
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6221)
AUTHORS Sladitschek HL, Neveu PA
TITLE Bidirectional Promoter Engineering for Single Cell MicroRNA Sensors
in Embryonic Stem Cells.
JOURNAL PLoS One. 2016 May 6;11(5):e0155177. doi:
10.1371/journal.pone.0155177. eCollection 2016.
PUBMED 27152616
REFERENCE 2 (bases 1 to 6221)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6221
/organism="synthetic DNA construct"
/mol_type="other DNA"
intron 101..1117
/label=chimeric intron
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
promoter complement(1118..1394)
/label=chicken beta-actin promoter
enhancer 1396..1775
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
enhancer 1783..2162
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
enhancer 2170..2549
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
enhancer 2557..2936
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 2946..3222
/label=chicken beta-actin promoter
intron 3223..4239
/label=chimeric intron
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
promoter 4329..4433
/gene="bla"
/label=AmpR promoter
CDS 4434..5294
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(4652..4671)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 5465..6053
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5954..5973
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 catgatatca aacgcgaagc ttacgcgtaa cctcgagcca tggaacgaat tctttgccaa
61 aatgatgaga cagcacaata accagcacgt tgcccaggag ctgtaggaaa aagaagaagg
121 catgaacatg gttagcagag gctctagagc cgccggtcac acgccagaag ccgaaccccg
181 ccctgccccg tcccccccga aggcagccgt ccccccgcgg acagccccga ggctggagag
241 ggagaagggg acggcggcgc ggcgacgcac gaaggccctc cccgcccatt tccttcctgc
301 cggcgccgca ccgcttcgcc ccgcgcccgc tagagggggt gcggcggcgc ctcccagatt
361 tcggctccgc acagatttgg gacaaaggaa gtccctgcgc cctctcgcac gattaccata
421 aaaggcaatg gctgcggctc gccgcgcctc gacagccgcc ggcgctccgg gggccgccgc
481 gcccctcccc cgagccctcc ccggcccgag gcggccccgc cccgcccggc acccccacct
541 gccgccaccc cccgcccggc acggcgagcc ccgcgccacg ccccgtacgg agccccgcac
601 ccgaagccgg gccgtgctca gcaactcggg gaggggggtg cagggggggt tgcagcccga
661 ccgacgcgcc cacaccccct gctcaccccc ccacgcacac accccgcacg cagcctttgt
721 tcccctcgca gccccccccg caccgcgggg caccgccccc ggccgcgctc ccctcgcgca
781 cactgcggag cgcacaaagc cccgcgccgc gcccgcagcg ctcacagccg ccgggcagcg
841 cggagccgca cgcggcgctc cccacgcaca cacacacgca cgcacccccc gagccgctcc
901 ccccgcacaa agggccctcc cggagcccct caaggctttc acgcagccac agaaaagaaa
961 caagccgtca ttaaaccaag cgctaattac agcccggagg agaagggccg tcccgcccgc
1021 tcacctgtgg gagtaacgcg gtcagtcaga gccggggcgg gcggcgcgag gcggcggcgg
1081 agcggggcac ggggcgaagg cagcgcgcag cgactcccgc ccgccgcgcg cttcgctttt
1141 tatagggccg ccgccgccgc cgcctcgcca taaaaggaaa ctttcggagc gcgccgctct
1201 gattggctgc cgccgcacct ctccgcctcg cccgccccgc ccctcgcccc gccccgcccc
1261 gcctggcgcg cgcccccccc ccccccccgc ccccatcgct gcacaaaata attaaaaaat
1321 aaataaatac aaaattgggg gtggggaggg gggggagatg gggagagtga agcagaacgt
1381 ggggctcacc tcgaccatgg taatagcgat gactaatacg tagatgtact gccaagtagg
1441 aaagtcccat aaggtcatgt actgggcata atgccaggcg ggccatttac cgtcattgac
1501 gtcaataggg ggcgtacttg gcatatgata cacttgatgt actgccaagt gggcagttta
1561 ccgtaaatac tccacccatt gacgtcaatg gaaagtccct attggcgtta ctatgggaac
1621 atacgtcatt attgacgtca atgggcgggg gtcgttgggc ggtcagccag gcgggccatt
1681 taccgtaagt tatgtaacgc ggaactccat atatgggcta tgaactaatg accccgtaat
1741 tgattactat taataactag tcaataatca atgtcgagaa cccatggtaa tagcgatgac
1801 taatacgtag atgtactgcc aagtaggaaa gtcccataag gtcatgtact gggcataatg
1861 ccaggcgggc catttaccgt cattgacgtc aatagggggc gtacttggca tatgatacac
1921 ttgatgtact gccaagtggg cagtttaccg taaatactcc acccattgac gtcaatggaa
1981 agtccctatt ggcgttacta tgggaacata cgtcattatt gacgtcaatg ggcgggggtc
2041 gttgggcggt cagccaggcg ggccatttac cgtaagttat gtaacgcgga actccatata
2101 tgggctatga actaatgacc ccgtaattga ttactattaa taactagtca ataatcaatg
2161 tcgagaaccc atggtaatag cgatgactaa tacgtagatg tactgccaag taggaaagtc
2221 ccataaggtc atgtactggg cataatgcca ggcgggccat ttaccgtcat tgacgtcaat
2281 agggggcgta cttggcatat gatacacttg atgtactgcc aagtgggcag tttaccgtaa
2341 atactccacc cattgacgtc aatggaaagt ccctattggc gttactatgg gaacatacgt
2401 cattattgac gtcaatgggc gggggtcgtt gggcggtcag ccaggcgggc catttaccgt
2461 aagttatgta acgcggaact ccatatatgg gctatgaact aatgaccccg taattgatta
2521 ctattaataa ctagtcaata atcaatgtcg agaacccatg gtaatagcga tgactaatac
2581 gtagatgtac tgccaagtag gaaagtccca taaggtcatg tactgggcat aatgccaggc
2641 gggccattta ccgtcattga cgtcaatagg gggcgtactt ggcatatgat acacttgatg
2701 tactgccaag tgggcagttt accgtaaata ctccacccat tgacgtcaat ggaaagtccc
2761 tattggcgtt actatgggaa catacgtcat tattgacgtc aatgggcggg ggtcgttggg
2821 cggtcagcca ggcgggccat ttaccgtaag ttatgtaacg cggaactcca tatatgggct
2881 atgaactaat gaccccgtaa ttgattacta ttaataacta gtcaataatc aatgtcgagc
2941 catggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc
3001 cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg
3061 gggggggcgc gcgccaggcg gggcggggcg gggcgagggg cggggcgggc gaggcggaga
3121 ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg
3181 cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cgggagtcgc tgcgcgctgc
3241 cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc
3301 gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg taattagcgc
3361 ttggtttaat gacggcttgt ttcttttctg tggctgcgtg aaagccttga ggggctccgg
3421 gagggccctt tgtgcggggg gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg
3481 agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg
3541 ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc
3601 ggggggggct gcgaggggaa caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca
3661 gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc acccccctcc ccgagttgct
3721 gagcacggcc cggcttcggg tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg
3781 ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg
3841 gagggctcgg gggaggggcg cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg
3901 agccgcagcc attgcctttt atggtaatcg tgcgagaggg cgcagggact tcctttgtcc
3961 caaatctgtg cggagccgaa atctgggagg cgccgccgca ccccctctag cgggcgcggg
4021 gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg agggccttcg tgcgtcgccg
4081 cgccgccgtc cccttctccc tctccagcct cggggctgtc cgcgggggga cggctgcctt
4141 cgggggggac ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc
4201 tctgctaacc atgttcatgc cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt
4261 attgtgctgt ctcatcattt tggcaaagaa ttcgtcgaca agcttctcga ccaattcggg
4321 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
4381 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
4441 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
4501 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
4561 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
4621 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
4681 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
4741 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
4801 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
4861 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
4921 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
4981 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
5041 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
5101 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
5161 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
5221 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
5281 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
5341 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
5401 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
5461 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
5521 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
5581 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
5641 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
5701 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
5761 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
5821 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
5881 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
5941 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
6001 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
6061 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
6121 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
6181 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag c
//
