| 质粒名称: | pBABEhygroHigheGFPhTERT |
|---|---|
| 出品公司： | Addgene |
| 目录编号: | 28169 |
| 存储实验室: | Kathleen Collins |
|
载体骨架基本信息 |
|
| 载体名称: | pBABEhygro |
| 空载体大小: | 5500 bp |
| 完整质粒大小: | 9347 bp |
| 载体类型: | 哺乳细胞表达、Retroviral |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | eGFPhTERT |
| 物种来源: | H. sapiens (human); eGFP |
| 插入大小: | 4250 bp |
| 启动子: | CMV |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | EcoRI(未破坏) |
| 3’克隆位点: | SalI (未破坏) |
| 5’测序引物 | n/a |
|
文献引用方法 |
|
| 材料和方法部分: |
pBABEhygroHigheGFPhTERT was a gift from Kathleen Collins (Addgene plasmid # 28169 ; http://n2t.net/addgene:28169 ; RRID:Addgene_28169)
|
| 参考文献部分: | Subnuclear shuttling of human telomerase induced by transformation and DNA damage. Wong JM, Kusdra L, Collins K. Nat Cell Biol. 2002 Sep . 4(9):731-6. 10.1038/ncb846 PubMed 12198499 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pBABEhygroHigheGFPhTERT 9347 bp ds-DNA circular SYN 25-AUG-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pBABEhygroHigheGFPhTERT
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9347)
AUTHORS Wong JM, Kusdra L, Collins K
TITLE Subnuclear shuttling of human telomerase induced by transformation
and DNA damage.
JOURNAL Nat Cell Biol. 2002 Sep . 4(9):731-6.
PUBMED 12198499
REFERENCE 2 (bases 1 to 9347)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 25, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..9347
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 1..470
/note="long terminal repeat from Moloney murine leukemia
virus"
misc_feature 535..734
/label=MMLV Psi
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
CDS 935..1351
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 1281..1303
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 1319..1335
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
regulatory 1389..1398
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 1395..2111
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS 1395..2111
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(1440..1461)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(1701..1720)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 2048..2069
/label=EGFP-C
/note="EGFP, forward primer"
CDS 2127..5525
/codon_start=1
/gene="TERT"
/product="human telomerase reverse transcriptase, the
catalytic subunit of telomerase"
/label=hTERT
/note="prevents telomere shortening and allows
postsenescent cells to proliferate beyond crisis (Counter
et al., 1998); also known as EST2"
/translation="MPRAPRCRAVRSLLRSHYREVLPLATFVRRLGPQGWRLVQRGDPA
AFRALVAQCLVCVPWDARPPPAAPSFRQVSCLKELVARVLQRLCERGAKNVLAFGFALL
DGARGGPPEAFTTSVRSYLPNTVTDALRGSGAWGLLLRRVGDDVLVHLLARCALFVLVA
PSCAYQVCGPPLYQLGAATQARPPPHASGPRRRLGCERAWNHSVREAGVPLGLPAPGAR
RRGGSASRSLPLPKRPRRGAAPEPERTPVGQGSWAHPGRTRGPSDRGFCVVSPARPAEE
ATSLEGALSGTRHSHPSVGRQHHAGPPSTSRPPRPWDTPCPPVYAETKHFLYSSGDKEQ
LRPSFLLSSLRPSLTGARRLVETIFLGSRPWMPGTPRRLPRLPQRYWQMRPLFLELLGN
HAQCPYGVLLKTHCPLRAAVTPAAGVCAREKPQGSVAAPEEEDTDPRRLVQLLRQHSSP
WQVYGFVRACLRRLVPPGLWGSRHNERRFLRNTKKFISLGKHAKLSLQELTWKMSVRGC
AWLRRSPGVGCVPAAEHRLREEILAKFLHWLMSVYVVELLRSFFYVTETTFQKNRLFFY
RKSVWSKLQSIGIRQHLKRVQLRELSEAEVRQHREARPALLTSRLRFIPKPDGLRPIVN
MDYVVGARTFRREKRAERLTSRVKALFSVLNYERARRPGLLGASVLGLDDIHRAWRTFV
LRVRAQDPPPELYFVKVDVTGAYDTIPQDRLTEVIASIIKPQNTYCVRRYAVVQKAAHG
HVRKAFKSHVSTLTDLQPYMRQFVAHLQETSPLRDAVVIEQSSSLNEASSGLFDVFLRF
MCHHAVRIRGKSYVQCQGIPQGSILSTLLCSLCYGDMENKLFAGIRRDGLLLRLVDDFL
LVTPHLTHAKTFLRTLVRGVPEYGCVVNLRKTVVNFPVEDEALGGTAFVQMPAHGLFPW
CGLLLDTRTLEVQSDYSSYARTSIRASLTFNRGFKAGRNMRRKLFGVLRLKCHSLFLDL
QVNSLQTVCTNIYKILLLQAYRFHACVLQLPFHQQVWKNPTFFLRVISDTASLCYSILK
AKNAGMSLGAKGAAGPLPSEAVQWLCHQAFLLKLTRHRVTYVPLLGSLRTAQTQLSRKL
PGTTLTALEAAANPALPSDFKTILD"
primer_bind complement(5537..5557)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 5542..5871
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 5722..5857
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 5784..5803
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 5945..6970
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
E"
primer_bind 7487..7506
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
LTR 7768..8237
/note="long terminal repeat from Moloney murine leukemia
virus"
CDS complement(8352..9212)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8975..8994
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9213..9317)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
61 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
121 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag
181 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
241 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
301 aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga gtcgcccggg
361 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
421 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct
481 cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg aggcaagctg
541 gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgactgatt ttatgcgcct
601 gcgtcggtac tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga
661 gttctgaaca cccggccgca accctgggag acgtcccagg gactttgggg gccgtttttg
721 tggcccgacc tgaggaaggg agtcgatgtg gaatccgacc ccgtcaggat atgtggttct
781 ggtaggagac gagaacctaa aacagttccc gcctccgtct gaatttttgc tttcggtttg
841 gaaccgaagc cgcgcgtctt gtctgctgca gcgctgcagc atcgttctgt gttgtctctg
901 tctgactgtg tttctgtatt tgtctgaaaa ttagggccag actgttacca ctcccttaag
961 tttgacctta ggtcactgga aagatgtcga gcggatcgct cacaaccagt cggtagatgt
1021 caagaagaga cgttgggtta ccttctgctc tgcagaatgg ccaaccttta acgtcggatg
1081 gccgcgagac ggcaccttta accgagacct catcacccag gttaagatca aggtcttttc
1141 acctggcccg catggacacc cagaccaggt cccctacatc gtgacctggg aagccttggc
1201 ttttgacccc cctccctggg tcaagccctt tgtacaccct aagcctccgc ctcctcttcc
1261 tccatccgcc ccgtctctcc cccttgaacc tcctcgttcg accccgcctc gatcctccct
1321 ttatccagcc ctcactcctt ctctaggcgc cggccggatc ccagtgtggt ggtacgtagg
1381 aattcgctgc caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
1441 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
1501 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
1561 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
1621 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
1681 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
1741 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
1801 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
1861 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
1921 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
1981 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
2041 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
2101 agctgtacaa gggagcaggt gctcatatgc cgcgcgctcc ccgctgccga gccgtgcgct
2161 ccctgctgcg cagccactac cgcgaggtgc tgccgctggc cacgttcgtg cggcgcctgg
2221 ggccccaggg ctggcggctg gtgcagcgcg gggacccggc ggctttccgc gcgctggtgg
2281 cccagtgcct ggtgtgcgtg ccctgggacg cacggccgcc ccccgccgcc ccctccttcc
2341 gccaggtgtc ctgcctgaag gagctggtgg cccgagtgct gcagaggctg tgcgagcgcg
2401 gcgcgaagaa cgtgctggcc ttcggcttcg cgctgctgga cggggcccgc gggggccccc
2461 ccgaggcctt caccaccagc gtgcgcagct acctgcccaa cacggtgacc gacgcactgc
2521 gggggagcgg ggcgtggggg ctgctgctgc gccgcgtggg cgacgacgtg ctggttcacc
2581 tgctggcacg ctgcgcgctc tttgtgctgg tggctcccag ctgcgcctac caggtgtgcg
2641 ggccgccgct gtaccagctc ggcgctgcca ctcaggcccg gcccccgcca cacgctagtg
2701 gaccccgaag gcgtctggga tgcgaacggg cctggaacca tagcgtcagg gaggccgggg
2761 tccccctggg cctgccagcc ccgggtgcga ggaggcgcgg gggcagtgcc agccgaagtc
2821 tgccgttgcc caagaggccc aggcgtggcg ctgcccctga gccggagcgg acgcccgttg
2881 ggcaggggtc ctgggcccac ccgggcagga cgcgtggacc gagtgaccgt ggtttctgtg
2941 tggtgtcacc tgccagaccc gccgaagaag ccacctcttt ggagggtgcg ctctctggca
3001 cgcgccactc ccacccatcc gtgggccgcc agcaccacgc gggcccccca tccacatcgc
3061 ggccaccacg tccctgggac acgccttgtc ccccggtgta cgccgagacc aagcacttcc
3121 tctactcctc aggcgacaag gagcagctgc ggccctcctt cctactcagc tctctgaggc
3181 ccagcctgac tggcgctcgg aggctcgtgg agaccatctt tctgggttcc aggccctgga
3241 tgccagggac tccccgcagg ttgccccgcc tgccccagcg ctactggcaa atgcggcccc
3301 tgtttctgga gctgcttggg aaccacgcgc agtgccccta cggggtgctc ctcaagacgc
3361 actgcccgct gcgagctgcg gtcaccccag cagccggtgt ctgtgcccgg gagaagcccc
3421 agggctctgt ggcggccccc gaggaggagg acacagaccc ccgtcgcctg gtgcagctgc
3481 tccgccagca cagcagcccc tggcaggtgt acggcttcgt gcgggcctgc ctgcgccggc
3541 tggtgccccc aggcctctgg ggctccaggc acaacgaacg ccgcttcctc aggaacacca
3601 agaagttcat ctccctgggg aagcatgcca agctctcgct gcaggagctg acgtggaaga
3661 tgagcgtgcg gggctgcgct tggctgcgca ggagcccagg ggttggctgt gttccggccg
3721 cagagcaccg tctgcgtgag gagatcctgg ccaagttcct gcactggctg atgagtgtgt
3781 acgtcgtcga gctgctcagg tctttctttt atgtcacgga gaccacgttt caaaagaaca
3841 ggctcttttt ctaccggaag agtgtctgga gcaagttgca aagcattgga atcagacagc
3901 acttgaagag ggtgcagctg cgggagctgt cggaagcaga ggtcaggcag catcgggaag
3961 ccaggcccgc cctgctgacg tccagactcc gcttcatccc caagcctgac gggctgcggc
4021 cgattgtgaa catggactac gtcgtgggag ccagaacgtt ccgcagagaa aagagggccg
4081 agcgtctcac ctcgagggtg aaggcactgt tcagcgtgct caactacgag cgggcgcggc
4141 gccccggcct cctgggcgcc tctgtgctgg gcctggacga tatccacagg gcctggcgca
4201 ccttcgtgct gcgtgtgcgg gcccaggacc cgccgcctga gctgtacttt gtcaaggtgg
4261 atgtgacggg cgcgtacgac accatccccc aggacaggct cacggaggtc atcgccagca
4321 tcatcaaacc ccagaacacg tactgcgtgc gtcggtatgc cgtggtccag aaggccgccc
4381 atgggcacgt ccgcaaggcc ttcaagagcc acgtctctac cttgacagac ctccagccgt
4441 acatgcgaca gttcgtggct cacctgcagg agaccagccc gctgagggat gccgtcgtca
4501 tcgagcagag ctcctccctg aatgaggcca gcagtggcct cttcgacgtc ttcctacgct
4561 tcatgtgcca ccacgccgtg cgcatcaggg gcaagtccta cgtccagtgc caggggatcc
4621 cgcagggctc catcctctcc acgctgctct gcagcctgtg ctacggcgac atggagaaca
4681 agctgtttgc ggggattcgg cgggacgggc tgctcctgcg tttggtggat gatttcttgt
4741 tggtgacacc tcacctcacc cacgcgaaaa ccttcctcag gaccctggtc cgaggtgtcc
4801 ctgagtatgg ctgcgtggtg aacttgcgga agacagtggt gaacttccct gtagaagacg
4861 aggccctggg tggcacggct tttgttcaga tgccggccca cggcctattc ccctggtgcg
4921 gcctgctgct ggatacccgg accctggagg tgcagagcga ctactccagc tatgcccgga
4981 cctccatcag agccagtctc accttcaacc gcggcttcaa ggctgggagg aacatgcgtc
5041 gcaaactctt tggggtcttg cggctgaagt gtcacagcct gtttctggat ttgcaggtga
5101 acagcctcca gacggtgtgc accaacatct acaagatcct cctgctgcag gcgtacaggt
5161 ttcacgcatg tgtgctgcag ctcccatttc atcagcaagt ttggaagaac cccacatttt
5221 tcctgcgcgt catctctgac acggcctccc tctgctactc catcctgaaa gccaagaacg
5281 cagggatgtc gctgggggcc aagggcgccg ccggccctct gccctccgag gccgtgcagt
5341 ggctgtgcca ccaagcattc ctgctcaagc tgactcgaca ccgtgtcacc tacgtgccac
5401 tcctggggtc actcaggaca gcccagacgc agctgagtcg gaagctcccg gggacgacgc
5461 tgactgccct ggaggccgca gccaacccgg cactgccctc agacttcaag accatcctgg
5521 actgagtcga ccctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
5581 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
5641 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
5701 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
5761 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
5821 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttgcc
5881 agctggggcg ccctctggta aggttgggaa gccctgcaga tctgatcccg gggggcaatg
5941 agatatgaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt
6001 cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt
6061 cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa
6121 agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga
6181 cattggggaa tttagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac
6241 gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg cggaggccat
6301 ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca
6361 aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt
6421 gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga
6481 tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt
6541 cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga
6601 ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt
6661 ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc
6721 gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt
6781 tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc
6841 cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga
6901 tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc
6961 aaaggaatag agtagatgcc gaccgggatc tatcgatgcg gcgggtgtgg tggttacgcg
7021 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
7081 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
7141 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
7201 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
7261 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
7321 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
7381 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
7441 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
7501 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
7561 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc atcgataaaa taaaagattt
7621 tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt ggcaagctag
7681 cttaagtaac gccattttgc aaggcatgga aaaatacata actgagaata gagaagttca
7741 gatcaaggtc aggaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa
7801 gcagttcctg ccccggctca gggccaagaa cagatggaac agctgaatat gggccaaaca
7861 ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggtccccaga
7921 tgcggtccag ccctcagcag tttctagaga accatcagat gtttccaggg tgccccaagg
7981 acctgaaatg accctgtgcc ttatttgaac taaccaatca gttcgcttct cgcttctgtt
8041 cgcgcgcttc tgctccccga gctcaataaa agagcccaca acccctcact cggggcgcca
8101 gtcctccgat tgactgagtc gcccgggtac ccgtgtatcc aataaaccct cttgcagttg
8161 catccgactt gtggtctcgc tgttccttgg gagggtctcc tctgagtgat tgactacccg
8221 tcagcggggg tctttcacat gcagcatgta tcaaaattaa tttggttttt tttcttaagt
8281 atttacatta aatggccata gtgcggccgc aaatcaatct aaagtatata tgagtaaact
8341 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
8401 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
8461 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
8521 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
8581 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
8641 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
8701 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
8761 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
8821 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
8881 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
8941 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
9001 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
9061 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
9121 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
9181 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
9241 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
9301 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgcagc
//
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