| 质粒名称: | pCL-MFG-BRCA1 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 12341 |
| 存储实验室: | Inder Verma |
|
载体骨架基本信息 |
|
| 载体名称: | pCLMFG |
| 载体出品公司: | Verma Lab |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，逆转录病毒 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | BRCA1 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | NcoI（部分摘要）（未销毁） |
| 3' 克隆位点: | XbaI / BamHI（在克隆过程中销毁） |
| 5' 测序引物 | LXSN primer |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pCL-MFG-BRCA1 was a gift from Inder Verma (Addgene plasmid # 12341 ; http://n2t.net/addgene:12341 ; RRID:Addgene_12341) |
| 参考文献部分: | BRCA1 is a cell cycle-regulated nuclear phosphoprotein. Ruffner H, Verma IM. Proc Natl Acad Sci U S A. 1997 Jul 8. 94(14):7138-43. 10.1073/pnas.94.14.7138 PubMed 9207057 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCL-MFG-BRCA1 12409 bp ds-DNA circular SYN 09-APR-2019
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pCL-MFG-BRCA1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12409)
AUTHORS Ruffner H, Verma IM
TITLE BRCA1 is a cell cycle-regulated nuclear phosphoprotein.
JOURNAL Proc Natl Acad Sci U S A. 1997 Jul 8. 94(14):7138-43.
PUBMED 9207057
REFERENCE 2 (bases 1 to 12409)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 9, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..12409
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 1..380
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 381..584
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 535..555
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
misc_feature 823..1180
/label=MMLV Psi
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
CDS 1245..1661
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 1591..1613
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 1629..1645
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
misc_feature 1671..2045
/label=pol region
/note="Moloney murine leukemia virus (MMLV) pol region
containing the splice acceptor site"
primer_bind 1983..1999
/label=pBMN 5'
/note="MMLV sequence, for inserts in pBMN retroviral
vector"
LTR 7889..8482
/note="long terminal repeat from Moloney murine leukemia
virus"
intron 8827..8892
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 9022..9042
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
polyA_signal 9314..9448
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(9351..9370)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 9405..9424
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 9906..10040
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 9968..9987
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(10105..10122)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(10276..10864)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(10356..10375)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(11035..11895)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 11658..11677
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(11896..12000)
/gene="bla"
/label=AmpR promoter
primer_bind 12068..12086
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind 12246..12265
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcaataa aagagcccac
601 aacccctcac tcggcgcgcc agtcctccga ttgactgagt cgcccgggta cccgtgtatc
661 caataaaccc tcttgcagtt gcatccgact tgtggtctcg ctgttccttg ggagggtctc
721 ctctgagtga ttgactaccc gtcagcgggg gtctttcatt tgggggctcg tccgggatcg
781 ggagacccct gcccagggac caccgaccca ccaccgggag gtaagctggc cagcaactta
841 tctgtgtctg tccgattgtc tagtgtctat gactgatttt atgcgcctgc gtcggtacta
901 gttagctaac tagctctgta tctggcggac ccgtggtgga actgacgagt tcggaacacc
961 cggccgcaac cctgggagac gtcccaggga cttcgggggc cgtttttgtg gcccgacctg
1021 agtccaaaaa tcccgatcgt tttggactct ttggtgcacc ccccttagag gagggatatg
1081 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
1141 cggtttggga ccgaagccgc gccgcgcgtc ttgtctgctg cagcatcgtt ctgtgttgtc
1201 tctgtctgac tgtgtttctg tatttgtctg aaaatatggg cccgggccag actgttacca
1261 ctcccttaag tttgacctta ggtcactgga aagatgtcga gcggatcgct cacaaccagt
1321 cggtagatgt caagaagaga cgttgggtta ccttctgctc tgcagaatgg ccaaccttta
1381 acgtcggatg gccgcgagac ggcaccttta accgagacct catcacccag gttaagatca
1441 aggtcttttc acctggcccg catggacacc cagaccaggt cccctacatc gtgacctggg
1501 aagccttggc ttttgacccc cctccctggg tcaagccctt tgtacaccct aagcctccgc
1561 ctcctcttcc tccatccgcc ccgtctctcc cccttgaacc tcctcgttcg accccgcctc
1621 gatcctccct ttatccagcc ctcactcctt ctctaggcgc ccccatatgg ccatatgaga
1681 tcttatatgg ggcacccccg ccccttgtaa acttccctga ccctgacatg acaagagtta
1741 ctaacagccc ctctctccaa gctcacttac aggctctcta cttagtccag cacgaagtct
1801 ggagacctct ggcggcagcc taccaagaac aactggaccg accggtggta cctcaccctt
1861 accgagtcgg cgacacagtg tgggtccgcc gacaccagac taagaaccta gaacctcgct
1921 ggaaaggacc ttacacagtc ctgctgacca cccccaccgc cctcaaagta gacggcatcg
1981 cagcttggat acacgccgcc cacgtgaagg ctgccgaccc cgggggtgga ccatcctcta
2041 gactgccatg gatttatctg ctcttcgcgt tgaagaagta caaaatgtca ttaatgctat
2101 gcagaaaatc ttagagtgtc ccatctgtct ggagttgatc aaggaacctg tctccacaaa
2161 gtgtgaccac atattttgca aattttgcat gctgaaactt ctcaaccaga agaaagggcc
2221 ttcacagtgt cctttatgta agaatgatat aaccaaaagg agcctacaag aaagtacgag
2281 atttagtcaa cttgttgaag agctattgaa aatcatttgt gcttttcagc ttgacacagg
2341 tttggagtat gcaaacagct ataattttgc aaaaaaggaa aataactctc ctgaacatct
2401 aaaagatgaa gtttctatca tccaaagtat gggctacaga aaccgtgcca aaagacttct
2461 acagagtgaa cccgaaaatc cttccttgca ggaaaccagt ctcagtgtcc aactctctaa
2521 ccttggaact gtgagaactc tgaggacaaa gcagcggata caacctcaaa agacgtctgt
2581 ctacattgaa ttgggatctg attcttctga agataccgtt aataaggcaa cttattgcag
2641 tgtgggagat caagaattgt tacaaatcac ccctcaagga accagggatg aaatcagttt
2701 ggattctgca aaaaaggctg cttgtgaatt ttctgagacg gatgtaacaa atactgaaca
2761 tcatcaaccc agtaataatg atttgaacac cactgagaag cgtgcagctg agaggcatcc
2821 agaaaagtat cagggtagtt ctgtttcaaa cttgcatgtg gagccatgtg gcacaaatac
2881 tcatgccagc tcattacagc atgagaacag cagtttatta ctcactaaag acagaatgaa
2941 tgtagaaaag gctgaattct gtaataaaag caaacagcct ggcttagcaa ggagccaaca
3001 taacagatgg gctggaagta aggaaacatg taatgatagg cggactccca gcacagaaaa
3061 aaaggtagat ctgaatgctg atcccctgtg tgagagaaaa gaatggaata agcagaaact
3121 gccatgctca gagaatccta gagatactga agatgttcct tggataacac taaatagcag
3181 cattcagaaa gttaatgagt ggttttccag aagtgatgaa ctgttaggtt ctgatgactc
3241 acatgatggg gagtctgaat caaatgccaa agtagctgat gtattggacg ttctaaatga
3301 ggtagatgaa tattctggtt cttcagagaa aatagactta ctggccagtg atcctcatga
3361 ggctttaata tgtaaaagtg aaagagttca ctccaaatca gtagagagta atattgaaga
3421 caaaatattt gggaaaacct atcggaagaa ggcaagcctc cccaacttaa gccatgtaac
3481 tgaaaatcta attataggag catttgttac tgagccacag ataatacaag agcgtcccct
3541 cacaaataaa ttaaagcgta aaaggagacc tacatcaggc cttcatcctg aggattttat
3601 caagaaagca gatttggcag ttcaaaagac tcctgaaatg ataaatcagg gaactaacca
3661 aacggagcag aatggtcaag tgatgaatat tactaatagt ggtcatgaga ataaaacaaa
3721 aggtgattct attcagaatg agaaaaatcc taacccaata gaatcactcg aaaaagaatc
3781 tgctttcaaa acgaaagctg aacctataag cagcagtata agcaatatgg aactcgaatt
3841 aaatatccac aattcaaaag cacctaaaaa gaataggctg aggaggaagt cttctaccag
3901 gcatattcat gcgcttgaac tagtagtcag tagaaatcta agcccaccta attgtactga
3961 attgcaaatt gatagttgtt ctagcagtga agagataaag aaaaaaaagt acaaccaaat
4021 gccagtcagg cacagcagaa acctacaact catggaaggt aaagaacctg caactggagc
4081 caagaagagt aacaagccaa atgaacagac aagtaaaaga catgacagcg atactttccc
4141 agagctgaag ttaacaaatg cacctggttc ttttactaag tgttcaaata ccagtgaact
4201 taaagaattt gtcaatccta gccttccaag agaagaaaaa gaagagaaac tagaaacagt
4261 taaagtgtct aataatgctg aagaccccaa agatctcatg ttaagtggag aaagggtttt
4321 gcaaactgaa agatctgtag agagtagcag tatttcattg gtacctggta ctgattatgg
4381 cactcaggaa agtatctcgt tactggaagt tagcactcta gggaaggcaa aaacagaacc
4441 aaataaatgt gtgagtcagt gtgcagcatt tgaaaacccc aagggactaa ttcatggttg
4501 ttccaaagat aatagaaatg acacagaagg ctttaagtat ccattgggac atgaagttaa
4561 ccacagtcgg gaaacaagca tagaaatgga agaaagtgaa cttgatgctc agtatttgca
4621 gaatacattc aaggtttcaa agcgccagtc atttgctctg ttttcaaatc caggaaatgc
4681 agaagaggaa tgtgcaacat tctctgccca ctctgggtcc ttaaagaaac aaagtccaaa
4741 agtcactttt gaatgtgaac aaaaggaaga aaatcaagga aagaatgagt ctaatatcaa
4801 gcctgtacag acagttaata tcactgcagg ctttcctgtg gttggtcaga aagataagcc
4861 agttgataat gccaaatgta gtatcaaagg aggctctagg ttttgtctat catctcagtt
4921 cagaggcaac gaaactggac tcattactcc aaataaacat ggacttttac aaaacccata
4981 tcgtatacca ccactttttc ccatcaagtc atttgttaaa actaaatgta agaaaaatct
5041 gctagaggaa aactttgagg aacattcaat gtcacctgaa agagaaatgg gaaatgagaa
5101 cattccaagt acagtgagca caattagccg taataacatt agagaaaatg tttttaaaga
5161 agccagctca agcaatatta atgaagtagg ttccagtact aatgaagtgg gctccagtat
5221 taatgaaata ggttccagtg atgaaaacat tcaagcagaa ctaggtagaa acagagggcc
5281 aaaattgaat gctatgctta gattaggggt tttgcaacct gaggtctata aacaaagtct
5341 tcctggaagt aattgtaagc atcctgaaat aaaaaagcaa gaatatgaag aagtagttca
5401 gactgttaat acagatttct ctccatatct gatttcagat aacttagaac agcctatggg
5461 aagtagtcat gcatctcagg tttgttctga gacacctgat gacctgttag atgatggtga
5521 aataaaggaa gatactagtt ttgctgaaaa tgacattaag gaaagttctg ctgtttttag
5581 caaaagcgtc cagaaaggag agcttagcag gagtcctagc cctttcaccc atacacattt
5641 ggctcagggt taccgaagag gggccaagaa attagagtcc tcagaagaga acttatctag
5701 tgaggatgaa gagcttccct gcttccaaca cttgttattt ggtaaagtaa acaatatacc
5761 ttctcagtct actaggcata gcaccgttgc taccgagtgt ctgtctaaga acacagagga
5821 gaatttatta tcattgaaga atagcttaaa tgactgcagt aaccaggtaa tattggcaaa
5881 ggcatctcag gaacatcacc ttagtgagga aacaaaatgt tctgctagct tgttttcttc
5941 acagtgcagt gaattggaag acttgactgc aaatacaaac acccaggatc cttttttgat
6001 tggttcttcc aaacaaatga ggcatcagtc tgaaagccag ggagttggtc tgagtgacaa
6061 ggaattggtt tcagatgatg aagaaagagg aacgggcttg gaagaaaata atcaagaaga
6121 gcaaagcatg gattcaaact taggtgaagc agcatctggg tgtgagagtg aaacaagcgt
6181 ctctgaagac tgctcagggc tatcctctca gagtgacatt ttaaccactc agcagaggga
6241 taccatgcaa cataacctga taaagctcca gcaggaaatg gctgaactag aagctgtgtt
6301 agaacagcat gggagccagc cttctaacag ctacccttcc atcataagtg actcttctgc
6361 ccttgaggac ctgcgaaatc cagaacaaag cacatcagaa aaagcagtat taacttcaca
6421 gaaaagtagt gaatacccta taagccagaa tccagaaggc ctttctgctg acaagtttga
6481 ggtgtctgca gatagttcta ccagtaaaaa taaagaacca ggagtggaaa ggtcatcccc
6541 ttctaaatgc ccatcattag atgataggtg gtacatgcac agttgctctg ggagtcttca
6601 gaatagaaac tacccatctc aagaggagct cattaaggtt gttgatgtgg aggagcaaca
6661 gctggaagag tctgggccac acgatttgac ggaaacatct tacttgccaa ggcaagatct
6721 agagggaacc ccttacctgg aatctggaat cagcctcttc tctgatgacc ctgaatctga
6781 tccttctgaa gacagagccc cagagtcagc tcgtgttggc aacataccat cttcaacctc
6841 tgcattgaaa gttccccaat tgaaagttgc agaatctgcc cagagtccag ctgctgctca
6901 tactactgat actgctgggt ataatgcaat ggaagaaagt gtgagcaggg agaagccaga
6961 attgacagct tcaacagaaa gggtcaacaa aagaatgtcc atggtggtgt ctggcctgac
7021 cccagaagaa tttatgctcg tgtacaagtt tgccagaaaa caccacatca ctttaactaa
7081 tctaattact gaagagacta ctcatgttgt tatgaaaaca gatgctgagt ttgtgtgtga
7141 acggacactg aaatattttc taggaattgc gggaggaaaa tgggtagtta gctatttctg
7201 ggtgacccag tctattaaag aaagaaaaat gctgaatgag catgattttg aagtcagagg
7261 agatgtggtc aatggaagaa accaccaagg tccaaagcga gcaagagaat cccaggacag
7321 aaagatcttc agggggctag aaatctgttg ctatgggccc ttcaccaaca tgcccacaga
7381 tcaactggaa tggatggtac agctgtgtgg tgcttctgtg gtgaaggagc tttcatcatt
7441 cacccttggc acaggtgtcc acccaattgt ggttgtgcag ccagatgcct ggacagagga
7501 caatggcttc catgcaattg ggcagatgtg tgaggcacct gtggtgaccc gagagtgggt
7561 gttggacagt gtagcactct accagtgcca ggagctggac acctacctga taccccagat
7621 cccccacagc cactactgac tgcagccagc cacaggtaca gagccacagg accccaagaa
7681 tgagcttaca aagtggcctt tccaggccct gggagaattc gagctcggta cccggggatc
7741 ctctagccgg attagtccaa tttgttaaag acaggatatc agtggtccag gctctagttt
7801 tgactcaaca atatcaccag ctgaagccta tagagtacga gccatagata aaataaaaga
7861 ttttatttag tctccagaaa aaggggggaa tgaaagaccc cacctgtagg tttggcaagc
7921 tagcttaagt aacgccattt tgcaaggcat ggaaaaatac ataactgaga atagagaagt
7981 tcagatcaag gtcaggaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg
8041 taagcagttc ctgccccggc tcagggccaa gaacagatgg aacagctgaa tatgggccaa
8101 acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggtcccc
8161 agatgcggtc cagccctcag cagtttctag agaaccatca gatgtttcca gggtgcccca
8221 aggacctgaa atgaccctgt gccttatttg aactaaccaa tcagttcgct tctcgcttct
8281 gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc acaacccctc actcggggcg
8341 ccagtcctcc gattgactga gtcgcccggg tacccgtgta tccaataaac cctcttgcag
8401 ttgcatccga cttgtggtct cgctgttcct tgggagggtc tcctctgagt gattgactac
8461 ccgtcagcgg gggtctttca tttgggggct cgtccgggat cgggagaccc ctgcccaggg
8521 accaccgacc caccaccggg aggtaagctg gctgcctcgc gcgtttcggt gatgacggtg
8581 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
8641 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
8701 tgacccagtc acgtagcgat agcggagtgt atctagctag gtagctagag gatctttgtg
8761 aaggaacctt acttctgtgg tgtgacataa ttggacaaac tacctacaga gatttaaagc
8821 tctaaggtaa atataaaatt tttaagtgta taatgtgtta aactactgat tctaattgtt
8881 tgtgtatttt agattccaac ctatggaact gatgaatggg agcagtggtg gaatgccttt
8941 aatgaggaaa acctgttttg ctcagaagaa atgccatcta gtgatgatga ggctactgct
9001 gactctcaac attctactcc tccaaaaaag aagagaaagg tagaagaccc caaggacttt
9061 ccttcagaat tgctaagttt tttgagtcat gctgtgttta gtaatagaac tcttgcttgc
9121 tttgctattt acaccacaaa ggaaaaagct gcactgctat acaagaaaat tatggaaaaa
9181 tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat ttgtagaggt
9241 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
9301 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
9361 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
9421 catcaatgta tcttatcatg tctggatcag cttcagaaga tggcggaggg cctccaacac
9481 agtaattttc ctcccgactc ttaaaataga aaatgtcaag tcagttaagc aggaagtgac
9541 taactgaccg cagctggccg tgcgacatcc tcttttaatt agttgctagg caactgccct
9601 ccagagggca gtgtggtttt gcaagaggaa gcaaaaagcc tctccaccca ggcctagaat
9661 gtttccaccc aatcattact atgacaacag ctgttttttt tagtattaag cagaggccgg
9721 gggcccctgg cctccgctta ctctggagaa aaagaagaga ggcattgtag aggcttccag
9781 aggcaacttg tcaaaacagg actgcttcta tttctgtcac actgtctggc cctgtcacaa
9841 ggtccagcac ctccataccc cctttaataa gcagtttggg aacgggtgcg ggtcttactc
9901 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
9961 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
10021 gaggaggctt ttttggaggc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
10081 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
10141 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
10201 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
10261 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
10321 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
10381 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
10441 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
10501 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
10561 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
10621 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
10681 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
10741 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
10801 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
10861 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
10921 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
10981 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
11041 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
11101 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
11161 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
11221 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
11281 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
11341 tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
11401 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
11461 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
11521 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
11581 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
11641 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
11701 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
11761 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
11821 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
11881 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
11941 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
12001 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
12061 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
12121 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
12181 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
12241 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
12301 cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg
12361 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagcgcgtt
//
