| 出品人: | Irene Castano Navarro（2015） |
|---|---|
| 载体名称: | pYC50 |
| 质粒类型: | 酿酒酵母表达载体；Recipient integrative Vector；FLP/FRT系统载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | -- |
| 载体大小: | 6481 bp |
| 5' 测序引物及序列: | M13/pUC fwd primer 5’ CCAGTCACGACGTTGTAAAACG 3’ |
| 3' 测序引物及序列: | -- |
| 载体标签: | 13xMyc tag |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | URA3，Nourseothricin |
| 克隆菌株: | DH10B感受态细胞 ,30°C |
| 宿主细胞（系）: | 出芽酵母（酿酒酵母）、白色念珠菌 |
| 备注: | -- |
| 产品目录号: | # 63906 |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pYC50载体质粒基本信息
pYC50质粒图谱载体图谱和pYC50载体序列质粒序列多克隆位点信息
pYC50质粒载体简介
pYC50质粒序列载体序列
LOCUS pYC50 6481 bp DNA SYN
DEFINITION pYC50
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..6481
/organism="pYC50"
/mol_type="other DNA"
misc_feature complement(29..51)
/label="pGEX_3_primer"
promoter 196..409
/label="URA3_promoter"
gene 411..1211
/label="URA3"
/gene="URA3"
CDS 411..1214
/label="ORF frame 3"
/translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL
LELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVK
LQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLS
TGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYR
TVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN*"
CDS complement(446..922)
/label="ORF frame 1"
/translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
misc_feature complement(1814..1962)
/label="lacZ_a"
/translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
misc_feature 1928..1950
/label="M13_pUC_fwd_primer"
/translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
promoter 1943..1959
/label="M13_forward20_primer"
/translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
promoter 1966..1984
/label="T7_promoter"
/translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
misc_feature 2017..2033
/label="pBluescriptSK_primer"
/translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
CDS complement(3287..3790)
/label="ORF frame 1"
/translation="MNSWSINIGNFQPQMTSTSFSGEFSSQTHQSSTNTMTSMFWSNF
NIFNSQSSVPTRIRNNNETSQITIITISNESSRIWITIFTSIGFIIGFIIWENLSQWW
INWNFSQSETITSSSNSKNSIGSERTIQSFNSFSITWNRSSVSISSIIQSSRHNCRID
PVVYVRM*"
CDS 3326..3898
/label="ORF frame 2"
/translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM
GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
promoter complement(4220..4239)
/label="T3_promoter"
/translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM
GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
promoter complement(4256..4274)
/label="M13_reverse_primer"
/translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM
GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
misc_feature complement(4273..4295)
/label="M13_pUC_rev_primer"
/translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM
GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
rep_origin complement(4647..5266)
/label="pBR322_origin"
/translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM
GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
gene complement(5421..6281)
/label="Ampicillin"
/gene="Ampicillin"
/translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM
GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
CDS complement(5421..6281)
/label="ORF frame 3"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
promoter complement(6323..6351)
/label="AmpR_promoter"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 Tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accacgcttt tcaattcaat tcatcatttt ttttttattc ttttttttga tttcggtttc
241 tttgaaattt ttttgattcg gtaatctccg aacagaagga agaacgaagg aaggagcaca
301 gacttagatt ggtatatata cgcatatgta gtgttgaaga aacatgaaat tgcccagtat
361 tcttaaccca actgcacaga acaaaaacct gcaggaaacg aagataaatc ATGTCGAAAG
421 CTACATATAA GGAACGTGCT GCTACTCATC CTAGTCCTGT TGCTGCCAAG CTATTTAATA
481 TCATGCACGA AAAGCAAACA AACTTGTGTG CTTCATTGGA TGTTCGTACC ACCAAGGAAT
541 TACTGGAGTT AGTTGAAGCA TTAGGTCCCA AAATTTGTTT ACTAAAAACA CATGTGGATA
601 TCTTGACTGA TTTTTCCATG GAGGGCACAG TTAAGCCGCT AAAGGCATTA TCCGCCAAGT
661 ACAATTTTTT ACTCTTCGAA GACAGAAAAT TTGCTGACAT TGGTAATACA GTCAAATTGC
721 AGTACTCTGC GGGTGTATAC AGAATAGCAG AATGGGCAGA CATTACGAAT GCACACGGTG
781 TGGTGGGCCC AGGTATTGTT AGCGGTTTGA AGCAGGCGGC AGAAGAAGTA ACAAAGGAAC
841 CTAGAGGCCT TTTGATGTTA GCAGAATTGT CATGCAAGGG CTCCCTATCT ACTGGAGAAT
901 ATACTAAGGG TACTGTTGAC ATTGCGAAGA GCGACAAAGA TTTTGTTATC GGCTTTATTG
961 CTCAAAGAGA CATGGGTGGA AGAGATGAAG GTTACGATTG GTTGATTATG ACACCCGGTG
1021 TGGGTTTAGA TGACAAGGGA GACGCATTGG GTCAACAGTA TAGAACCGTG GATGATGTGG
1081 TCTCTACAGG ATCTGACATT ATTATTGTTG GAAGAGGACT ATTTGCAAAG GGAAGGGATG
1141 CTAAGGTAGA GGGTGAACGT TACAGAAAAG CAGGCTGGGA AGCATATTTG AGAAGATGCG
1201 GCCAGCAAAA CTAAaaaact gtattataag taaatgcatg tatactaaac tcacaaatta
1261 gagcttcaat ttaattatat cagttattac cctgcggtgt gaaataccgc acagatgcgt
1321 aaggagaaaa taccgcatca ggaaattgta aacgttaata ttttgttaaa attcgcgtta
1381 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
1441 aaatcaaaag aatagaccga gatagggttg agtgTTGTTC CAGTTTGGAA CAAGAGTCCA
1501 CTATTAAAGA ACGTGGACTC CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC
1561 CCACTACGTG AACCATCACC CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA
1621 AATCGGAACC CTAAAGGGAG CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG
1681 GCGAGAAAGG AAGGGAAGAA AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG
1741 GTCACGCTGC GCGTAACCAC CACACCCGCC GCGCTTAATG Cgccgctaca gggcgcgtcg
1801 cgCCATTCGC CATTCAGGCT GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG
1861 CTATTACGCC AGCTGGCGAA GGGGGGATGT GCTGCAAGGC GATTAAGTTG GGTAACGCCA
1921 GGGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCCAGTG AAttgtaata cgactcacta
1981 tagggcgaat tggagctcca ccgcggtggc ggccgctcta gaactagtgg atcctagggT
2041 TAATTAACGG TGAACAAAAG CTAATCTCCG AGGAAGACTT GAACGGTGAA CAAAAATTAA
2101 TCTCAGAAGA AGACTTGAAC GGACTCGACG GTGAACAAAA GTTGATTTCT GAAGAAGATT
2161 TGAACGGTGA ACAAAAGCTA ATCTCCGAGG AAGACTTGAA CGGTGAACAA AAATTAATCT
2221 CAGAAGAAGA CTTGAACGGA CTCGACGGTG AACAAAAGTT GATTTCTGAA GAAGATTTGA
2281 ACGGTGAACA AAAGCTAATC TCCGAGGAAG ACTTGAACGG TGAACAAAAA TTAATCTCAG
2341 AAGAAGACTT GAACGGACTC GACGGTGAAC AAAAGTTGAT TTCTGAAGAA GATTTGAACG
2401 GTGAACAAAA GCTAATCTCC GAGGAAGACT TGAACGGTGA ACAAAAATTA ATCTCAGAAG
2461 AAGACTTGAA CGGACTCGAC GGTGAACAAA AGTTGATTTC TGAAGAAGAT TTGAACGGTG
2521 AACAAAAGCT AATCTCCGAG GAAGACTTGA ACGGTGAACA AAAATTAATC AATCACtagc
2581 tagtgaattc gcgccagatc cGAAGTTCCT ATACTTTCTA GAGAATAGGA ACTTCgtgcg
2641 cttttgaacc acgtaaagtg ctgtaaaaca gctctattca atgaggtaaa tgataaagtt
2701 tataagagac caaaaaattc aaaaaaaaaa ttgaaaaatt atattggtat ttatatgaaa
2761 caatagttca gttcaaagcg ctagcttttc tcatctcaac taaccggtat taggtgggta
2821 tttatttaat atgggaacaa gaaatgtgac actaatgttc atgtttttat tttcattatt
2881 tattctatat ggtttgggac ttgatgttat gtcttaattc atagaGATCC gcttgcctcg
2941 tccccgccgg gtcacccggc cagcgacatg gaggcccaga ataccctcct tgacagtctt
3001 gacgtgcgca gctcaggggc atgatgtgac tgtcgcccgt acatttagcc catacatccc
3061 catgtataat catttgcatc catacatttt gatggccgca cggcgcgaag caaaaattac
3121 ggctcctcgc tgcagacctg cgagcaggga aacgctcccc tcacagacgc gttgaattgt
3181 ccccacgccg cgcccctgta gagaaatata aaaggttagg atttgccact gaggttcttc
3241 tttcatatac ttccttttaa aatcttgcta ggatacagtt ctcacatcac atccgaacat
3301 aaacaaccgG ATCgatccta caattATGTC TACTACTTTG GATGATACTG CTTATAGATA
3361 CAGAACTTCT GTTCCAGGTG ATGCTGAAGC TATTGAAGCT TTGGATGGTT CTTTCACTAC
3421 CGATACTGTT TTTAGAGTTA CTGCTACTGG TGATGGTTTC ACTTTGAGAG AAGTTCCAGT
3481 TGATCCACCA TTGACTAAGG TTTTCCCAGA TGATGAATCC GATGATGAAT CCGATGCTGG
3541 TGAAGATGGT GATCCAGATT CTAGAACTTT CGTTGCTTAT GGTGATGATG GTGATTTGGC
3601 TGGTTTCGTT GTTGTTTCTT ATTCTGGTTG GAACAGAAGA TTGACTGTTG AAGATATTGA
3661 AGTTGCTCCA GAACATAGAG GTCATGGTGT TGGTAGAGCT TTGATGGGTT TGGCTACTGA
3721 ATTCGCCAGA GAAAGAGGTG CTGGTCATTT GTGGTTGGAA GTTACCAATG TTAATGCTCC
3781 AGCTATTCAT GCTTATAGAA GAATGGGTTT CACTTTGTGT GGTTTGGATA CTGCTTTATA
3841 CGATGGTACT GCTTCCGATG GTGAACAAGC TTTGTATATG TCTATGCCAT GTCCATAAta
3901 acTCGACcag tactgacaat aaaaagattc ttgttttcaa gaacttgtca tttgtatagt
3961 ttttttatat tgtagttgtt ctattttaat caaatgttag cgtgatttat attttttttc
4021 gcctcgacat catctgccca gatgcgaagt taagtgcgca gaaagtaata tcatgcgtca
4081 atcgtatgtg aatgctggtc gctatactgc tgtcgattcg atactaacgc cgccatccag
4141 tgTCGAtcga cGAAGTTCCT ATACTTTCTA GAGAATAGGA ACTTCGTCct cgaggggggg
4201 cccggtacca gcttttgttc cctttagtga gggttaattc cgagcttggc gtaatcatgg
4261 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa cataggagcc
4321 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga ggtaactcac attaattgcg
4381 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
4441 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
4501 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
4561 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
4621 caaaaggcca ggaaccgtaa aaaggcCGCG TTGCTGGCGT TTTTCCATAG GCTCGGCCCC
4681 CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA
4741 TAAAGATACC AGGCGTTCCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG
4801 CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCAATGC
4861 TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC
4921 GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC
4981 CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG
5041 AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA
5101 AGGACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT
5161 AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG
5221 CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTtttc tacggggtct
5281 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
5341 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
5401 gagtaaactt ggtctgacag TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC
5461 TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTGCCCGTCG TGTAGATAAC TACGATACGG
5521 GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT
5581 CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA
5641 ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG
5701 CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG
5761 TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC
5821 CCCATGTTGT GAAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG
5881 TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG
5941 CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG
6001 TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT
6061 AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG
6121 ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA
6181 GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA
6241 AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA Tactcttcct ttttcaatat
6301 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
6361 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
6421 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
6481 c
//
