| 质粒名称: | pTE-10M0X |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 20318 |
| 存储实验室: | Dirk Schnappinger |
|
载体骨架基本信息 |
|
| 载体名称: | pTE-mcs |
| 载体出品公司: | -- |
| 载体抗性: | 潮霉素 |
| 空载体大小: | 5133 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 细菌表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 低拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Pimyc-tetR#10 |
| 物种来源: | Tn10 tetR合成基因 |
| 插入大小: | 970 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | PacI(未破坏) |
| 3' 克隆位点: | NotI (未破坏) |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pTE-10M0X was a gift from Dirk Schnappinger (Addgene plasmid # 20318 ; http://n2t.net/addgene:20318 ; RRID:Addgene_20318) |
| 参考文献部分: | Improved tetracycline repressors for gene silencing in mycobacteria. Klotzsche M, Ehrt S, Schnappinger D. Nucleic Acids Res. 2009 Jan 27. ():. 10.1093/nar/gkp015 PubMed 19174563 |
买家导航
pTE10M0X载体基本信息
pTE10M0X载体图谱质粒图谱和多克隆位点信息
pTE10M0X载体简介
pTE10M0X载体序列
LOCUS pTE-10M0X 6103 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pTE-10M0X
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6103)
AUTHORS Klotzsche M, Ehrt S, Schnappinger D
TITLE Improved tetracycline repressors for gene silencing in mycobacteria.
JOURNAL Nucleic Acids Res. 2009 Jan 27. ():.
PUBMED 19174563
REFERENCE 2 (bases 1 to 6103)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6103
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 38..54
/label=pBluescriptKS
/note="For pBluescript vector"
primer_bind 39..55
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
terminator 1056..1083
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
primer_bind complement(1173..1190)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(1344..1932)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(1424..1443)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 aacgttctcg gctcgatgat ccccggcggg ccccccctcg aggtcgacgg tatcgattta
61 attaatatgc atcaattgat ttaaatacta gtgttaacta tttaattggg gaccctagag
121 gtcccctttt ttattttaaa aattttttcn aaggggacct ctagggtccc cagctggcta
181 gcgagtcatg aggtnaccat ggtcattagg agccgctctc gcacttcagc tgcttctcca
241 agccgcagat gatcagctcc aggccgaaca agaaggccgg ctcggcgccc tggtggtcga
301 acagctcgat ggcctggcgc agcaacggcg gcatgctgtc ggtggtcggg gtctcgcgct
361 cctccttggc gacctggtgc tcctggtcct ccaacacgca gcccagggtg aagtggccca
421 cggccgacag ggcgtacaac gcgttctcca gcgagaagcc ctgctggcac aggaaggcca
481 actggttctc cagggtctcg tactgcttct cggtcggccg ggtgcccagg tgcaccttgg
541 cgccgtcgcg gtggctcagc aaggcgcagc ggaagctctt ggcgttgttg cgcaggaagt
601 cctgccagct ctcgccctcc agcgggcaga agtgggtgtg gtggcggtcc aacatctcga
661 tggccagggc gtcgagcagg gcccgcttgt tcttgacgtg ccagtacagg gtcggctgct
721 cgacgcccag cttctgggcc agcttgcggg tggtcaggcc ctcgatgccg acctcgttca
781 gcagctccag ggcggagttg atgaccttgg acttgtccag gcgggacatt gcgaagtgat
841 tcctcctgat ggtaccggat ccgtggcagg agggtcgcct gcgtcggcaa ctcgcccagg
901 ctacacgaga gccctcgact gcgaaaatgc cgaccaggca ccaccgcact ccaggcttaa
961 cccggtgcga cgatccaata ttacgagatc tgatatcctc gaggcatgcc acgtgcctgc
1021 aggtttaaac gcggccgcaa acagtattaa acgcaaaaag gccatccgtc aggatggcct
1081 tctgcttagc taatcaacta gttctagctg cattaatgaa tcggccaacg cgcggggaga
1141 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
1201 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
1261 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
1321 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
1381 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
1441 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
1501 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
1561 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
1621 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
1681 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
1741 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
1801 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
1861 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
1921 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
1981 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
2041 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
2101 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
2161 atagttgcct gactccccgg acgtggccga ccagcccgtc atcgtcaacg cctgaccgcg
2221 gtgcggacag gccgtgtcgc gaccggccgt gcggaattaa gccggcccgt accctgtgaa
2281 tagaggtccg ctgtgacaca agaatccctg ttacttctcg accgtattga ttcggatgat
2341 tcctacgcga gcctgcggaa cgaccaggaa ttctgggagc cgctggcccg ccgagccctg
2401 gaggagctcg ggctgccggt gccgccggtg ctgcgggtgc ccggcgagag caccaacccc
2461 gtactggtcg gcgagcccga cccggtgatc aagctgttcg gcgagcactg gtgcggtccg
2521 gagagcctcg cgtcggagtc ggaggcgtac gcggtcctgg cggacgcccc ggtgccggtg
2581 ccccgcctcc tcggccgcgg cgagctgcgg cccggcaccg gagcctggcc gtggccctac
2641 ctggtgatga gccggatgac cggcaccacc tggcggtccg cgatggacgg cacgaccgac
2701 cggaacgcgc tgctcgccct ggcccgcgaa ctcggccggg tgctcggccg gctgcacagg
2761 gtgccgctga ccgggaacac cgtgctcacc ccccattccg aggtcttccc ggaactgctg
2821 cgggaacgcc gcgcggcgac cgtcgaggac caccgcgggt ggggctacct ctcgccccgg
2881 ctgctggacc gcctggagga ctggctgccg gacgtggaca cgctgctggc cggccgcgaa
2941 ccccggttcg tccacggcga cctgcacggg accaacatct tcgtggacct ggccgcgacc
3001 gaggtcaccg ggatcgtcga cttcaccgac gtctatgcgg gagactcccg ctacagcctg
3061 gtgcaactgc atctcaacgc cttccggggc gaccgcgaga tcctggccgc gctgctcgac
3121 ggggcgcagt ggaagcggac cgaggacttc gcccgcgaac tgctcgcctt caccttcctg
3181 cacgacttcg aggtgttcga ggagaccccg ctggatctct ccggcttcac cgatccggag
3241 gaactggcgc agttcctctg ggggccgccg gacaccgccc ccggcgcctg acgccccggg
3301 ccgcccggcg ccgcccccgg cccccggcgg ccggccgccc ggagccccgc ccgcgctcgg
3361 gagccccggg cccgcgccga agcccgctgc tgcgagcccg gagcgggccg gccgacggcg
3421 gtgcgggccc ggcggcggac gctcagcagc ggcgggcgtg aaaggccctg gcatcctcga
3481 tcatctcctc cagggtggtc ggccgagctt ccatcccagc tcggcaagga tcaacgagga
3541 cagtcgcacg acgaagttct tctggatcgc gcccgtgctg gaagcactca acctcgaagc
3601 gtgtggttgc ggagccatct agcaaccaca cgaaacatgc gcaacgaacc gcgcaacgaa
3661 caacgcctag aactggccct agatgagctg actcgtatcg ttggtaaacc tagtttgacc
3721 agcatgtttt aactacgttc ggtgagctgt caacggggcc tgtaacggca caacgaaccg
3781 tgcaacgaga gtggccacgg atgccaccac aggcactaca acggagttcg ccacgtacat
3841 caccacaacc accgattctg gcggtgagct ccccgatatt cagcggaaat ggcttggtat
3901 cgaccaagat tcgtagaacc ccgtctcgtc tggctggtat tcaaaacggg cgcaacgaaa
3961 cacgcaacga gacaggcatg gcccaaacca gaaaactagc gtctaccagg acttttacct
4021 gtccgacccg ttgcaacgga accccccacg gaacccccgc gacacccgct ccccaattgc
4081 gttagaacag cggtggattg tcggcttcgt tgtgggcctt ttgagccgct tcctgttctg
4141 ccgcacgctc tttcctcgcc cgatagccga gtcgcttaac ggtgtccaga tgcagcccga
4201 aatgtttggc cgtttgcggc caagagtggc cctcgtcgtc gtgataggcg cggatgcgtt
4261 cgcggcgtgc agcctgctcg gcgagccact cgctgcgttc ctgcgccacg agccggacga
4321 cgtggcgttc ggatagtccg gtgattcgag cgccttcggc ggcggtcacg cgccgctttt
4381 tgcggacagt cggctgccgg ttgtagccgt cgctgtagcc gtcgctgtag ccgtcgctca
4441 tagcaatgcc tccatggctg acgcggactt tgcgcgccgc gcaactgtgc tcgccgccgt
4501 gcgcgctgct gcgcccttcc gcgagatggc cgactggcgc gcactgagtg tggcctcgta
4561 gaccacgatc ccgtccgccc aaatgcgcga cttggttgtg atccaacgcc aaatgctgtt
4621 ggcgatggcg cggacctcgc tgtccggtag cggtccggga cacacgtcgt tgcacgggaa
4681 ttcggcgttt cgcgcgtggc actcggcata gatcgcgcgg ccgagtccgt ccacgttccg
4741 ggtcggcagg tagatccgca tgagggcggg acgataggcc cacaacctga cggaatcgaa
4801 cagtgcgcaa ttccgcccta gcggcgtcgg agccgctttg tacgtggtct gctgacgcca
4861 gcgcggcggt ggcatgttcg cgccgagctc ggcctcgatg tggctgagtg tgtagagatc
4921 tgagtggagc cattccgttt cccaggcgat gtggccgggg tttttggtca tgaggcctga
4981 gtaactgcgg tcgccgtcga cggcgcgccg aaggccttcg gcgcacgccg ccatgtatgc
5041 gagcggctta cgccgcgcgt attcggtgcg tggaacaggg gcgttgagtg cccacactgc
5101 gtgtgcgtgg ccgttggcgc gattgcccac gatcgcgttg ggcagcggat gggacccccg
5161 ggcgctgagc gctcggagcg ctgcgtctgg atggtctacg tccacgacca gcaggtttgc
5221 cagcgctgtt gggttcgcct cgatgtaccg gcggcctagg gccgacgcgc ggctttggcg
5281 gtagatcccc tcgagcagat cgtcgcttgc cagcggccag tacggcagcc agagctgctc
5341 aaattcgtcg gcgacgtggc tcacgcttgg tagtagacca cgattaatca ccggtgtatg
5401 gtccgacacg agctccaagt cagatatttc gctgaggggc caccccacaa ctgcacactc
5461 ccccgctctc ccgtcgagcc ctggtggtgg aacaccagcg acagccgagc acccccaacc
5521 acctgtacca accaggagga acacatgcgt cgtttcgagg acgtttccgg gccgctgaga
5581 gccgctgtgg cggccgtaca cgccgcctta gacccgttag accccctgcc gcctgaatgc
5641 gcgggtacga gccacacagc gcccgaactt acggagctgg tgggctcacc tggctttatg
5701 gcgtacgaat cggctgtgtg cgacctgttg ggcgaggtga ggtacgcgct actcacgctg
5761 gcaagggcga cacagccgcc ccaccgagcc cgcacggccg cgcgcggtgt caacaaccgg
5821 gtgagtcgtg cacaccagca ggtgttcgag gcttggctcg aagtgcagga catcgtggcg
5881 aacgccgccc gatgagccgc gccttacgct ggctgccagc cgttcgcggg ctggttggtg
5941 cagcgcgtcg agcggttaga ggccctgcgg tgttccacca ccgcaggcct cgcccttttt
6001 aaggctgaat ttgcttgtct ccgaatccaa ctggcttgtc caagggtgta tctacgctta
6061 atccaaagtt caaacgaggg gattacacat gaccaacttc gat
//
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