| 质粒名称: | pHR_PGK_antiCD19_synNotch_Gal4VP64 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 79125 |
| 存储实验室: | Wendell Lim |
|
载体骨架基本信息 |
|
| 载体名称: | pHR PGK |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 慢病毒载体 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | antiCD19_synNotch_Gal4VP64 |
| 物种来源: | 合成 |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pHR_PGK_antiCD19_synNotch_Gal4VP64 was a gift from Wendell Lim (Addgene plasmid # 79125 ; http://n2t.net/addgene:79125 ; RRID:Addgene_79125) |
| 参考文献部分: | Engineering Customized Cell Sensing and Response Behaviors Using Synthetic Notch Receptors. Morsut L, Roybal KT, Xiong X, Gordley RM, Coyle SM, Thomson M, Lim WA. Cell. 2016 Feb 11;164(4):780-91. doi: 10.1016/j.cell.2016.01.012. Epub 2016 Jan 28. 10.1016/j.cell.2016.01.012 PubMed 26830878 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pHR_PGK_antiCD19_synNotch_Gal4VP64 11449 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Lentiviral vector for constitutive expression of the anti-CD19 scFv
synNotch Gal4DBDVP64 Receptor (PGK promoter).
ACCESSION .
VERSION .
KEYWORDS pHR_PGK_antiCD19_synNotch_Gal4VP64
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11449)
AUTHORS Morsut L, Roybal KT, Xiong X, Gordley RM, Coyle SM, Thomson M, Lim
WA
TITLE Engineering Customized Cell Sensing and Response Behaviors Using
Synthetic Notch Receptors.
JOURNAL Cell. 2016 Feb 11;164(4):780-91. doi: 10.1016/j.cell.2016.01.012.
Epub 2016 Jan 28.
PUBMED 26830878
REFERENCE 2 (bases 1 to 11449)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..11449
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 276..295
/label=mPGK-F
/note="Mouse PGK promoter, forward primer"
CDS 451..480
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 2662..2811
/codon_start=1
/product="tetrameric repeat of the minimal activation
domain of herpes simplex virus VP16 (Beerli et al., 1998)"
/label=VP64
/translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
DLDML"
misc_feature 2930..3518
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(2983..3003)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(3401..3412)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(3521..3537)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(3522..3538)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 3628..3861
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
promoter complement(3886..3904)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(3887..3904)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(4194..4213)
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
primer_bind complement(4427..4446)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 4546..4568
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(4606..4624)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 4692..4796
/gene="bla"
/label=AmpR promoter
CDS 4797..5657
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(5015..5034)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 5828..6416
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 6317..6336
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 6570..6587
/label=L4440
/note="L4440 vector, forward primer"
primer_bind complement(6657..6677)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 6662..6991
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 6842..6977
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 6904..6923
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
intron 8125..8190
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 8320..8340
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
polyA_signal 8765..8899
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(8802..8821)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 8856..8875
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
LTR 9067..9700
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 9747..9872
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 10369..10602
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 11116..11233
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 11293..343
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
primer_bind complement(11328..11351)
/label=MSCV-rev
/note="Murine stem cell virus, reverse primer"
ORIGIN
1 accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag
61 gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga
121 gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt
181 ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg
241 cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac
301 gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgaattctc acgcgtcaag
361 tggagcaagg caggtggaca gtggatcatg gcgctccctg tcaccgcact gcttcttccg
421 ctggcactgc tgctgcacgc tgcacggcct gagcaaaaac ttatctctga agaggacctc
481 gatatacaga tgacgcagac aacgtcaagt ctttccgcca gcttgggaga ccgagtgact
541 atatcttgta gagcaagcca ggatatttct aagtatctta actggtacca acaaaagccc
601 gatggaacgg ttaagctgct tatataccat accagtagac tccactccgg cgtaccatca
661 cggttttctg gcagtggctc cgggaccgac tattctttga cgatctctaa tctcgaacaa
721 gaggatattg caacatactt ttgtcagcaa ggcaatacct tgccatatac gtttgggggc
781 gggacaaaac ttgagataac cggcggcggt ggttcaggcg gtggcggttc cggtggtggg
841 ggatcagagg ttaagcttca ggaatccgga ccaggtttgg ttgcccccag ccaatctctc
901 agcgttacat gcacggtttc aggcgtcagt ctccccgatt acggtgtaag ttggattcgg
961 caacctccgc gaaagggtct ggaatggctg ggggttattt gggggagtga gacaacttat
1021 tacaactctg cacttaagag tcggcttacc atcatcaagg ataattcaaa atcacaagta
1081 ttcctgaaga tgaactcatt gcaaacagat gatacagcta tatactattg tgccaagcat
1141 tactattatg gtggttctta tgcaatggat tactgggggc aaggcacgtc agtgacagtg
1201 agttcaatcc tggactacag cttcacaggt ggcgctgggc gcgacattcc cccaccgcag
1261 attgaggagg cctgtgagct gcctgagtgc caggtggatg caggcaataa ggtctgcaac
1321 ctgcagtgta ataatcacgc atgtggctgg gatggtggcg actgctccct caacttcaat
1381 gacccctgga agaactgcac gcagtctcta cagtgctgga agtattttag cgacggccac
1441 tgtgacagcc agtgcaactc ggccggctgc ctctttgatg gcttcgactg ccagctcacc
1501 gagggacagt gcaaccccct gtatgaccag tactgcaagg accacttcag tgatggccac
1561 tgcgaccagg gctgtaacag tgccgaatgt gagtgggatg gcctagactg tgctgagcat
1621 gtacccgagc ggctggcagc cggcaccctg gtgctggtgg tgctgcttcc acccgaccag
1681 ctacggaaca actccttcca ctttctgcgg gagctcagcc acgtgctgca caccaacgtg
1741 gtcttcaagc gtgatgcgca aggccagcag atgatcttcc cgtactatgg ccacgaggaa
1801 gagctgcgca agcacccaat caagcgctct acagtgggtt gggccacctc ttcactgctt
1861 cctggtacca gtggtgggcg ccagcgcagg gagctggacc ccatggacat ccgtggctcc
1921 attgtctacc tggagatcga caaccggcaa tgtgtgcagt catcctcgca gtgcttccag
1981 agtgccaccg atgtggctgc cttcctaggt gctcttgcgt cacttggcag cctcaatatt
2041 ccttacaaga ttgaggccgt gaagagtgag ccggtggagc ctccgctgcc ctcgcagctg
2101 cacctcatgt acgtggcagc ggccgccttc gtgctcctgt tctttgtggg ctgtggggtg
2161 ctgctgtccc gcaagcgccg gcggatgaag ctgctgagca gcatcgagca ggcctgtgac
2221 atctgccggc tgaagaaact gaagtgcagc aaagaaaagc ccaagtgcgc caagtgcctg
2281 aagaacaact gggagtgccg gtacagcccc aagaccaaga gaagccccct gaccagagcc
2341 cacctgaccg aggtggaaag ccggctggaa agactggaac agctgtttct gctgatcttc
2401 ccacgcgagg acctggacat gatcctgaag atggacagcc tgcaggacat caaggccctg
2461 ctgaccggcc tgttcgtgca ggacaacgtg aacaaggacg ccgtgaccga cagactggcc
2521 agcgtggaaa ccgacatgcc cctgaccctg cggcagcaca gaatcagcgc caccagcagc
2581 agcgaggaaa gcagcaacaa gggccagcgg cagctgacag tgtctgctgc tgcaggcgga
2641 agcggaggct ctggcggatc tgatgccctg gacgacttcg acctggatat gctgggcagc
2701 gacgccctgg atgattttga tctggacatg ctgggatctg acgctctgga cgatttcgat
2761 ctcgacatgt tgggatcaga tgcactggat gactttgacc tggacatgct cggatcatga
2821 gatccttgac ttgcggccgc aactcccacc tgcaacatgc gtgactgact gaggccgcga
2881 ctctagagtc gacctgcagg catgcaagct tgatatcaag cttatcgata atcaacctct
2941 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
3001 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
3061 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
3121 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
3181 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
3241 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
3301 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc
3361 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
3421 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
3481 tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc gacctcgagg
3541 gaattaattc gagctcggta cctttaagac caatgactta caaggcagct gtagatctta
3601 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
3661 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
3721 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
3781 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
3841 cagtgtggaa aatctctagc agcatctaga attaattccg tgtattctat agtgtcacct
3901 aaatcgtatg tgtatgatac ataaggttat gtattaattg tagccgcgtt ctaacgacaa
3961 tatgtacaag cctaattgtg tagcatctgg cttactgaag cagaccctat catctctctc
4021 gtaaactgcc gtcagagtcg gtttggttgg acgaaccttc tgagtttctg gtaacgccgt
4081 cccgcacccg gaaatggtca gcgaaccaat cagcagggtc atcgctagcc agatcctcta
4141 cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat
4201 cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt
4261 cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca
4321 tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct
4381 aatgcaggag tcgcataagg gagagcgtcg aatggtgcac tctcagtaca atctgctctg
4441 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
4501 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
4561 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
4621 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
4681 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
4741 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
4801 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
4861 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
4921 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
4981 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
5041 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
5101 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
5161 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
5221 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
5281 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
5341 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
5401 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
5461 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
5521 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
5581 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
5641 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
5701 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
5761 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
5821 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
5881 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
5941 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
6001 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
6061 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
6121 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
6181 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
6241 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
6301 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
6361 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
6421 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
6481 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
6541 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
6601 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctgtggaa
6661 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
6721 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
6781 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
6841 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
6901 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
6961 aggctttttt ggaggcctag gcttttgcaa aaagcttgga cacaagacag gcttgcgaga
7021 tatgtttgag aataccactt tatcccgcgt cagggagagg cagtgcgtaa aaagacgcgg
7081 actcatgtga aatactggtt tttagtgcgc cagatctcta taatctcgcg caacctattt
7141 tcccctcgaa cactttttaa gccgtagata aacaggctgg gacacttcac atgagcgaaa
7201 aatacatcgt cacctgggac atgttgcaga tccatgcacg taaactcgca agccgactga
7261 tgccttctga acaatggaaa ggcattattg ccgtaagccg tggcggtctg taccgggtgc
7321 gttactggcg cgtgaactgg gtattcgtca tgtcgatacc gtttgtattt ccagctacga
7381 tcacgacaac cagcgcgagc ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt
7441 catcgttatt gatgacctgg tggataccgg tggtactgcg gttgcgattc gtgaaatgta
7501 tccaaaagcg cactttgtca ccatcttcgc aaaaccggct ggtcgtccgc tggttgatga
7561 ctatgttgtt gatatcccgc aagatacctg gattgaacag ccgtgggata tgggcgtcgt
7621 attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc ctggcactgc cgggcgttgt
7681 tctttttaac ttcaggcggg ttacaatagt ttccagtaag tattctggag gctgcatcca
7741 tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct
7801 cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg gcccttgatg
7861 gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct gatgtggatc tggcgcggca
7921 ttgacccacg cgaaatcctc gacgtccagg cacgtattgt gatgagcgat gccgaacgta
7981 ccgacgatga tttatacgat acggtgattg gctaccgtgg cggcaactgg atttatgagt
8041 gggccccgga tctttgtgaa ggaaccttac ttctgtggtg tgacataatt ggacaaacta
8101 cctacagaga tttaaagctc taaggtaaat ataaaatttt taagtgtata atgtgttaaa
8161 ctactgattc taattgtttg tgtattttag attccaacct atggaactga tgaatgggag
8221 cagtggtgga atgcctttaa tgaggaaaac ctgttttgct cagaagaaat gccatctagt
8281 gatgatgagg ctactgctga ctctcaacat tctactcctc caaaaaagaa gagaaaggta
8341 gaagacccca aggactttcc ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt
8401 aatagaactc ttgcttgctt tgctatttac accacaaagg aaaaagctgc actgctatac
8461 aagaaaatta tggaaaaata ttctgtaacc tttataagta ggcataacag ttataatcat
8521 aacatactgt tttttcttac tccacacagg catagagtgt ctgctattaa taactatgct
8581 caaaaattgt gtacctttag ctttttaatt tgtaaagggg ttaataagga atatttgatg
8641 tatagtgcct tgactagaga tcataatcag ccataccaca tttgtagagg ttttacttgc
8701 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
8761 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
8821 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
8881 atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc
8941 caccccatac cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga
9001 ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt
9061 agtagttgga agggctaatt cactcccaaa gaagacaaga tatccttgat ctgtggatct
9121 accacacaca aggctacttc cctgattagc agaactacac accagggcca ggggtcagat
9181 atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag
9241 aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg
9301 acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg
9361 cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac
9421 tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc
9481 tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag
9541 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
9601 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
9661 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
9721 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
9781 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
9841 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
9901 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
9961 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
10021 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
10081 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
10141 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
10201 cacagcaagc ggccggccgc tgatcttcag acctggagga ggagatatga gggacaattg
10261 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
10321 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
10381 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt
10441 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
10501 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
10561 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
10621 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
10681 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
10741 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
10801 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
10861 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
10921 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
10981 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
11041 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc gccaaatggc
11101 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
11161 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
11221 aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttgga tcgataagct
11281 tgatatcgaa ttgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag
11341 cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat
11401 ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcc
//
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