| 质粒名称: | pAnc80L65 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 68837 |
| 存储实验室: | Luk Vandenberghe |
|
载体骨架基本信息 |
|
| 载体名称: | synthetic |
| 载体出品公司: | -- |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | 5238 bp |
| 完整质粒大小: | 7446 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，AAV，合成生物学 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Ancestral AAV Capsid Anc80L65,ANC80L65 |
| 物种来源: | 合成的 |
| 插入大小: | 2208 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | Rep2 |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | M13rev |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pAnc80L65 was a gift from Luk Vandenberghe (Addgene plasmid # 68837 ; http://n2t.net/addgene:68837 ; RRID:Addgene_68837) |
| 参考文献部分: | In Silico Reconstruction of the Viral Evolutionary Lineage Yields a Potent Gene Therapy Vector. Zinn E, Pacouret S, Khaychuk V, Turunen HT, Carvalho LS, Andres-Mateos E, Shah S, Shelke R, Maurer AC, Plovie E, Xiao R, Vandenberghe LH. Cell Rep. 2015 Jul 28. pii: S2211-1247(15)00759-7. doi: 10.1016/j.celrep.2015.07.019. 10.1016/j.celrep.2015.07.019 PubMed 26235624 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pAnc80L65 7446 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Adeno-associated Viral Vector (AAV) capsid Anc80L65 in AAV2Rep
expression construct.
ACCESSION .
VERSION .
KEYWORDS pAnc80L65
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7446)
AUTHORS Zinn E, Pacouret S, Khaychuk V, Turunen HT, Carvalho LS,
Andres-Mateos E, Shah S, Shelke R, Maurer AC, Plovie E, Xiao R,
Vandenberghe LH
TITLE In Silico Reconstruction of the Viral Evolutionary Lineage Yields a
Potent Gene Therapy Vector.
JOURNAL Cell Rep. 2015 Jul 28. pii: S2211-1247(15)00759-7. doi:
10.1016/j.celrep.2015.07.019.
PUBMED 26235624
REFERENCE 2 (bases 1 to 7446)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..7446
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(2862..2878)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(2862..2878)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(3023..3040)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(3194..3782)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(3274..3293)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
terminator 4013..4040
/label=T7Te terminator
/note="phage T7 early transcription terminator"
CDS complement(4059..4874)
/codon_start=1
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
primer_bind 4782..4801
/label=Kan-R
/note="Kanamycin resistance gene, reverse primer"
primer_bind 4866..4885
/label=pENTR-R
/note="pENTR vectors, reverse primer"
terminator 5091..5118
/label=T7Te terminator
/note="phage T7 early transcription terminator"
rep_origin complement(5154..5609)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5291..5312)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 5503..5522
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 5736..5758
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 5750..5767
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 5751..5767
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 5801..5817
/label=pBluescriptKS
/note="For pBluescript vector"
primer_bind 5802..5818
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 agcttcgatc aactacgcag acaggtacca aaacaaatgt tctcgtcacg tgggcatgaa
61 tctgatgctg tttccctgca gacaatgcga gagaatgaat cagaattcaa atatctgctt
121 cactcacgga cagaaagact gtttagagtg ctttcccgtg tcagaatctc aacccgtttc
181 tgtcgtcaaa aaggcgtatc agaaactgtg ctacattcat catatcatgg gaaaggtgcc
241 agacgcttgc actgcctgcg atctggtcaa tgtggatttg gatgactgca tctttgaaca
301 ataaatgatt taaatcaggt atggctgccg atggttatct tccagattgg ctcgaggaca
361 acctctctga gggcattcgc gagtggtggg acttgaaacc tggagccccg aaacccaaag
421 ccaaccagca aaagcaggac gacggccggg gtctggtgct tcctggctac aagtacctcg
481 gacccttcaa cggactcgac aagggggagc ccgtcaacgc ggcggacgca gcggccctcg
541 agcacgacaa ggcctacgac cagcagctca aagcgggtga caatccgtac ctgcggtata
601 accacgccga cgccgagttt caggagcgtc tgcaagaaga tacgtctttt gggggcaacc
661 tcgggcgagc agtcttccag gccaagaagc gggttctcga acctctcggt ctggttgagg
721 aaggcgctaa gacggctcct ggaaagaaga ggccggtaga gcaatcaccc caggaaccag
781 actcctcttc gggcatcggc aagaaaggcc agcagcccgc gagaaagaga ctcaactttg
841 ggcagacagg cgactcagag tcagtgcccg accctcaacc actcggagaa ccccccgcag
901 ccccctctgg tgtgggatct aatacaatgg ctgcaggcgg tggcgctcca atggcagaca
961 ataacgaagg cgccgacgga gtgggtaacg cctcaggaaa ttggcattgc gattccacat
1021 ggctgggcga cagagtcatc accaccagca cccgaacctg ggccctcccc acctacaaca
1081 accacctcta caagcaaatc tccagccaat cgggaggcag caccaacgac aacacctact
1141 tcggctacag caccccctgg gggtattttg actttaacag attccactgc cacttctcac
1201 cacgtgactg gcagcgactc atcaacaaca actggggatt ccggcccaag aagctcaact
1261 tcaagctctt caacatccag gtcaaggagg tcacgacgaa tgatggcacc acgaccatcg
1321 ccaataacct taccagcacg gttcaggtct ttacggactc ggaataccag ctcccgtacg
1381 tcctcggctc tgcgcaccag ggctgcctgc ctccgttccc ggcggacgtc ttcatgattc
1441 ctcagtacgg gtacctgact ctgaacaatg gcagtcaggc cgtgggccgt tcctccttct
1501 actgcctgga gtactttcct tctcaaatgc tgagaacggg caacaacttt cagttcagct
1561 acacgtttga ggacgtgcct tttcacagca gctacgcgca cagccaaagc ctggaccggc
1621 tgatgaaccc cctcatcgac cagtacctgt actacctgtc tcggactcag accacgagtg
1681 gtaccgcagg aaatcggacg ttgcaatttt ctcaggccgg gcctagtagc atggcgaatc
1741 aggccaaaaa ctggctaccc gggccctgct accggcagca acgcgtctcc aagacaacca
1801 atcaaaataa caacagcaac tttgcctgga ccggtgccac caagtatcat ctgaatggca
1861 gagactctct ggtaaatccc ggtcccgcta tggcaaccca caaggacgac gaagacaaat
1921 tttttccgat gagcggagtc ttaatatttg ggaaacaggg agctggaaat agcaacgtgg
1981 accttgacaa cgttatgata accaacgagg aagaaattaa aaccaccaac ccagtggcca
2041 cagaagagta cggcacggtg gccactaacc tgcaatcggc caacaccgct cctgctacag
2101 ggaccgtcaa cagtcaagga gccttacctg gcatggtctg gcaggaccgg gacgtgtacc
2161 tgcagggtcc tatctgggcc aagattcctc acacggacgg acactttcat ccctcgccgc
2221 tgatgggagg ctttggactg aaacacccgc ctcctcagat cctgattaag aatacacctg
2281 ttcccgcgaa tcctccaact accttcagtc cagctaagtt tgcgtcgttc atcacgcagt
2341 acagcaccgg acaggtcagc gtggaaattg aatgggagct gcagaaagaa aacagcaaac
2401 gctggaaccc agagattcaa tacacttcca actacaacaa atctacaaat gtggactttg
2461 ctgttgacac aaatggcgtt tattctgagc ctcgccccat cggcacccgt tacctcaccc
2521 gtaatctgta aactagtttg cttgttaatc aataaaccgt ttaattcgtt tcagttgaac
2581 tttggtctct gcgtatttct ttcttatcta gtttccatgc tctagaggtc ctgtattaga
2641 ggtcacgtga gtgttttgcg acattttgcg acaccatgtg gtcacgctgg gtatttaagc
2701 ccgagtgagc acgcagggtc tccattttga agcgggaggt ttgaacgcgc agccgccaag
2761 ccgaattctg cagatatcca tcacactggc ggccgctcga ctagagcggc cgccaccgcg
2821 gtggagctcc agcttttgtt cgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg
2881 tgaaattcca cagcctgggg tgcctaattg cgttgcgctc actgcccgct ttccagtcgg
2941 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
3001 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
3061 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
3121 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
3181 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
3241 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
3301 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
3361 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
3421 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
3481 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
3541 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
3601 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
3661 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
3721 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
3781 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
3841 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
3901 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agaaataata
3961 aaaaagccgg attaataatc tggcttttta tattctctct ctagtatata aacgcagaaa
4021 ggcccacccg aaggtgagcc agtgtgactc tagtattatt agaaaaactc atcgagcatc
4081 aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt
4141 ttctgtaatg aaggagaaaa ctcaccgagg cagttccaaa gaatggcaag gtcctggtaa
4201 cggtctgcga ttccgacccg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa
4261 ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaag
4321 agcttgtgca tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa
4381 tcactcgcat caaccaaacc gttattcatg cgtgattgcg cctgagcaag acgaaataca
4441 cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gtaaccggcg caggaacacg
4501 gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaaggct
4561 gttttcccag gaatcgcggt ggtgagtaac cacgcatcat caggagtacg gataaaatgc
4621 ttgatggtcg ggagaggcat aaactccgtc agccagttga gacggaccat ctcatctgta
4681 acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc
4741 ccatacaagc gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac
4801 ccatataaat cagcgtccat gttggagttt aagcgcggac gggagcaaga cgtttcccgt
4861 tgaatatggc tcataacacc ccttgtatta ctgtttatgt aagcagacag ttttattgtt
4921 catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga cacaacgtgg
4981 ctttgttgaa taaatcgaac ttttgctgag ttgaaggatc agctctagta aaataataaa
5041 aaagccggat taataatctg gctttttata ttctctctct agtatataaa cgcagaaagg
5101 cccacccgaa ggtgagccag tgtgacggca catttccccg aaaagtgcca cctaaattgt
5161 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
5221 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
5281 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
5341 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
5401 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
5461 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
5521 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
5581 cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg
5641 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
5701 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
5761 ggccagtgag cgcgcggcga attgggtacc gggccccccc tcgaggtcga cggtatcgta
5821 tttaagcccg agtgagcacg cagggtctcc attttgaagc gggaggtttg aacgcgcagc
5881 cgccatgccg gggttttacg agattgtgat taaggtcccc agcgaccttg acgagcatct
5941 gcccggcatt tctgacagct ttgtgaactg ggtggccgag aaggaatggg agttgccgcc
6001 agattctgac atggatctga atctgattga gcaggcaccc ctgaccgtgg ccgagaagct
6061 gcagcgcgac tttctgacgg aatggcgccg tgtgagtaag gccccggagg ctcttttctt
6121 tgtgcaattt gagaagggag agagctactt ccacatgcac gtgctcgtgg aaaccaccgg
6181 ggtgaaatcc atggttttgg gacgtttcct gagtcagatt cgcgaaaaac tgattcagag
6241 aatttaccgc gggatcgagc cgactttgcc aaactggttc gcggtcacaa agaccagaaa
6301 tggcgccgga ggcgggaaca aggtggtgga tgagtgctac atccccaatt acttgctccc
6361 caaaacccag cctgagctcc agtgggcgtg gactaatatg gaacagtatt taagcgcctg
6421 tttgaatctc acggagcgta aacggttggt ggcgcagcat ctgacgcacg tgtcgcagac
6481 gcaggagcag aacaaagaga atcagaatcc caattctgat gcgccggtga tcagatcaaa
6541 aacttcagcc aggtacatgg agctggtcgg gtggctcgtg gacaagggga ttacctcgga
6601 gaagcagtgg atccaggagg accaggcctc atacatctcc ttcaatgcgg cctccaactc
6661 gcggtcccaa atcaaggctg ccttggacaa tgcgggaaag attatgagcc tgactaaaac
6721 cgcccccgac tacctggtgg gccagcagcc cgtggaggac atttccagca atcggattta
6781 taaaattttg gaactaaacg ggtacgatcc ccaatatgcg gcttccgtct ttctgggatg
6841 ggccacgaaa aagttcggca agaggaacac catctggctg tttgggcctg caactaccgg
6901 gaagaccaac atcgcggagg ccatagccca cactgtgccc ttctacgggt gcgtaaactg
6961 gaccaatgag aactttccct tcaacgactg tgtcgacaag atggtgatct ggtgggagga
7021 ggggaagatg accgccaagg tcgtggagtc ggccaaagcc attctcggag gaagcaaggt
7081 gcgcgtggac cagaaatgca agtcctcggc ccagatagac ccgactcccg tgatcgtcac
7141 ctccaacacc aacatgtgcg ccgtgattga cgggaactca acgaccttcg aacaccagca
7201 gccgttgcaa gaccggatgt tcaaatttga actcacccgc cgtctggatc atgactttgg
7261 gaaggtcacc aagcaggaag tcaaagactt tttccggtgg gcaaaggatc acgtggttga
7321 ggtggagcat gaattctacg tcaaaaaggg tggagccaag aaaagacccg cccccagtga
7381 cgcagatata agtgagccca aacgggtgcg cgagtcagtt gcgcagccat cgacgtcaga
7441 cgcgga
//
