| 出品公司: | Invitrogen |
|---|---|
| 载体名称: | pIZ/V5-His |
| 质粒类型: | 昆虫表达载体 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 启动子: | OplE2 |
| 载体大小: | 2876 bp |
| 5' 测序引物及序列: | OpIE2 Forward：CGCAACGATCTGGTAAACAC |
| 3' 测序引物及序列: | 5´-GACAATACAAACTAAGATTTAGTCAG-3´ |
| 载体标签: | V5 Epitope Tag , His Tag (C-末端) |
| 载体抗性: | Zeocin |
| 真核筛选标记: | Zeocin |
| 克隆菌株: | TOP10；DH5 |
| 宿主细胞（系）: | Sf9, Sf21，High Five |
| 备注: | -- |
| 产品目录号: | V8000-01 |
| 瞬表达/稳表达: | 瞬表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pIZ/V5-His载体质粒基本信息
pIZ/V5-His质粒图谱载体图谱和pIZ/V5-His载体序列质粒序列多克隆位点信息
pIZ/V5-His质粒载体简介
pIZ/V5-His质粒序列载体序列
LOCUS pIZ/V5-His 2876 bp DNA SYN
DEFINITION pIZ/V5-His
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..2876
/organism="pIZ/V5-His"
/mol_type="other DNA"
misc_feature 515..534
/label="OpIE2_fwd_primer"
rep_origin complement(991..1610)
/label="pBR322_origin"
misc_feature 2176..2195
/label="OpIE2_fwd_primer"
promoter 2281..2299
/label="T7_promoter"
gene 2309..2680
/label="bleo"
/gene="bleo"
misc_feature 2309..2683
/label="sh_ble"
terminator 2750..5
/label="SV40_PA_terminator"
misc_feature 2838..2857
/label="EBV_rev_primer"
ORIGIN
1 GGATCATGAT GATAAACAAT GTATGGTGCT AATGTTGCTT CAACAACAAT TCTGTTGAAC
61 TGTGTTTTCA TGTTTGCCAA CAAGCACCTT TATACTCGGT GGCCTCCCCA CCACCAACTT
121 TTTTGCACTG CAAAAAAACA CGCTTTTGCA CGCGGGCCCA TACATAGTAC AAACTCTACG
181 TTTCGTAGAC TATTTTACAT AAATAGTCTA CACCGTTGTA TACGCTCCAA ATACACTACC
241 ACACATTGAA CCTTTTTGCA GTGCAAAAAA GTACGTGTCG GCAGTCACGT AGGCCGGCCT
301 TATCGGGTCG CGTCCTGTCA CGTACGAATC ACATTATCGG ACCGGACGAG TGTTGTCTTA
361 TCGTGACAGG ACGCCAGCTT CCTGTGTTGC TAACCGCAGC CGGACGCAAC TCCTTATCGG
421 AACAGGACGC GCCTCCATAT CAGCCGCGCG TTATCTCATG CGCGTGACCG GACACGAGGC
481 GCCCGTCCCG CTTATCGCGC CTATAAATAC AGCCCGCAAC GATCTGGTAA ACACAGTTGA
541 ACAGCATCTG TTCGAATTTA AAGCTTGGTA CCGAGCTCGG ATCCACTAGT CCAGTGTGGT
601 GGAATTCTGC AGATATCCAG CACAGTGGCG GCCGCTCGAG TCTAGAGGGC CCGCGGTTCG
661 AAGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGCGTACC GGTCATCATC
721 ACCATCACCA TTGAGTTTAT CTGACTAAAT CTTAGTTTGT ATTGTCATGT TTTAATACAA
781 TATGTTATGT TTAAATATGT TTTTAATAAA TTTTATAAAA TAATTTCAAC TTTTATTGTA
841 ACAACATTGT CCATTTACAC ACTCCTTTCA AGCGCGTGGG ATCGATGCTC ACTCAAAGGC
901 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG
961 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG
1021 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG
1081 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC
1141 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA
1201 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT
1261 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC
1321 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG
1381 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC
1441 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT
1501 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA
1561 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG
1621 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA TGATAAACAA
1681 TGTATGGTGC TAATGTTGCT TCAACAACAA TTCTGTTGAA CTGTGTTTTC ATGTTTGCCA
1741 ACAAGCACCT TTATACTCGG TGGCCTCCCC ACCACCAACT TTTTTGCACT GCAAAAAAAC
1801 ACGCTTTTGC ACGCGGGCCC ATACATAGTA CAAACTCTAC GTTTCGTAGA CTATTTTACA
1861 TAAATAGTCT ACACCGTTGT ATACGCTCCA AATACACTAC CACACATTGA ACCTTTTTGC
1921 AGTGCAAAAA AGTACGTGTC GGCAGTCACG TAGGCCGGCC TTATCGGGTC GCGTCCTGTC
1981 ACGTACGAAT CACATTATCG GACCGGACGA GTGTTGTCTT ATCGTGACAG GACGCCAGCT
2041 TCCTGTGTTG CTAACCGCAG CCGGACGCAA CTCCTTATCG GAACAGGACG CGCCTCCATA
2101 TCAGCCGCGC GTTATCTCAT GCGCGTGACC GGACACGAGG CGCCCGTCCC GCTTATCGCG
2161 CCTATAAATA CAGCCCGCAA CGATCTGGTA AACACAGTTG AACAGCATCT GTTCGAATTA
2221 ATTCGGATCT CTGCAGCACG TGTTGACAAT TAATCATCGG CATAGTATAT CGGCATAGTA
2281 TAATACGACT CACTATAGGA GGGCCACCAT GGCCAAGTTG ACCAGTGCCG TTCCGGTGCT
2341 CACCGCGCGC GACGTCGCCG GAGCGGTCGA GTTCTGGACC GACCGGCTCG GGTTCTCCCG
2401 GGACTTCGTG GAGGACGACT TCGCCGGTGT GGTCCGGGAC GACGTGACCC TGTTCATCAG
2461 CGCGGTCCAG GACCAGGTGG TGCCGGACAA CACCCTGGCC TGGGTGTGGG TGCGCGGCCT
2521 GGACGAGCTG TACGCCGAGT GGTCGGAGGT CGTGTCCACG AACTTCCGGG ACGCCTCCGG
2581 GCCGGCCATG ACCGAGATCG GCGAGCAGCC GTGGGGGCGG GAGTTCGCCC TGCGCGACCC
2641 GGCCGGCAAC TGCGTGCACT TCGTGGCCGA GGAGCAGGAC TGACCGACGC CGACCAACAC
2701 CGCCGGTCCG ACGGCGGCCC ACGGGTCCCA GGGGGGTCGA CCTCGAAACT TGTTTATTGC
2761 AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT
2821 TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCT
//
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pIZ/V5-His质粒载体应用举例
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