pTrcHis2 A pTrcHis2A质粒图谱、载体图谱、序列、价格、抗性、测序引物、大小等信息

出品公司:	Invitrogen
别名:	pTrcHis2A, pTrcHis2 A
质粒类型:	大肠杆菌表达载体
表达水平:	高拷贝
启动子:	Trc
克隆方法:	多克隆位点，限制性内切酶
载体大小:	4406 bp
5' 测序引物及序列:	pTrcHis-F: gaggtatatattaatgtatcg
3' 测序引物及序列:	pTrcHis-R:GATTTAATCTGTATCAGG
载体标签:	C-His, C-Myc
载体抗性:	Ampicillin
备注:	Hosts: E.coli NM522, E.coli INValphaF'. Related vectors: pSE420. 适合高效表达真核基因。
产品目录号:	V365-20
稳定性:	瞬时表达 Transient
组成型:	组成型 Constitutive
病毒/非病毒:	非病毒

pTrcHis2A载体图谱

The pTrcHis2 plasmids are pBR322-derived expression vectors designed for efficient recombinant protein expression and purification in E. coli. High levels of expression are possible using the trc (trp-lac) promoter (Egon et al., 1983) and the rrnB anti-termination region (Li et al., 1984). The trc promoter contains the –35 region of the trp promoter together with the –10 region of the lac promoter (Brosius et al., 1985; Egon et al., 1983; Mulligan et al., 1985). To regulate expression, the gene encoding Lac repressor (lacIq) is provided in the pTrcHis2 vectors, allowing regulation of the trc promoter regardless of whether the host strain contains a gene encoding the Lac repressor.

Isopropyl-Beta-D-thiogalactopyranoside (IPTG) is used to induce expression of your gene. Translation is enhanced by the bacteriophage T7 gene 10 translation enhancer and a minicistron that provides highly efficient translational restart into the open reading frame of the multiple cloning site. DNA inserts are positioned downstream and in frame with the initiation ATG and a C-terminal fusion peptide. The C-terminal peptide encodes the myc epitope and six histidine residues that function as a metal binding site in the expressed protein.

LOCUS       pTrcHis2 A   4406 bp    DNA circular    SYN
DEFINITION pTrcHis2 A
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     source          1..4406
                     /organism="pTrcHis2 A"
                     /mol_type="other DNA"
     misc_feature    235..257
                     /label="M13_pUC_rev_primer"
     misc_feature    264..333
                     /label="rrnB_ATS"
     misc_feature    370..390
                     /label="pTrcHis_fwd_primer"
     misc_feature    383..409
                     /label="minicis"
     misc_feature    complement(589..606)
                     /label="pBAD_rev_primer"
     misc_feature    complement(589..606)
                     /label="pTrcHis_rev_primer"
     terminator      639..796
                     /label="rrnB_terminator"
     terminator      762..805
                     /label="rrnB_T1_terminator"
     terminator      937..964
                     /label="rrnB_T2_terminator"
     promoter        1006..1034
                     /label="AmpR_promoter"
     gene            1076..1936
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             1076..1936
                     /label="ORF frame 2"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      2091..2710
                     /label="pBR322_origin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    3107..3129
                     /label="pGEX_3_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    3276..4367
                     /label="lacI"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     CDS             3408..4367
                     /label="ORF frame 3"
                     /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
                     RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
                     PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
                     HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
                     SGLRVGADISVVGYDDTEDSSCYIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
                     LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
ORIGIN
    1 GTTTGACAGC TTATCATCGA CTGCACGGTG CACCAATGCT TCTGGCGTCA GGCAGCCATC
   61 GGAAGCTGTG GTATGGCTGT GCAGGTCGTA AATCACTGCA TAATTCGTGT CGCTCAAGGC
121 GCACTCCCGT TCTGGATAAT GTTTTTTGCG CCGACATCAT AACGGTTCTG GCAAATATTC
181 TGAAATGAGC TGTTGACAAT TAATCATCCG GCTCGTATAA TGTGTGGAAT TGTGAGCGGA
241 TAACAATTTC ACACAGGAAA CAGCGCCGCT GAGAAAAAGC GAAGCGGCAC TGCTCTTTAA
301 CAATTTATCA GACAATCTGT GTGGGCACTC GACCGGAATT ATCGATTAAC TTTATTATTA
361 AAAATTAAAG AGGTATATAT TAATGTATCG ATTAAATAAG GAGGAATAAA CCATGGATCC
421 GAGCTCGAGA TCTGCAGCTG GTACCATATG GGAATTCGAA GCTTGGGCCC GAACAAAAAC
481 TCATCTCAGA AGAGGATCTG AATAGCGCCG TCGACCATCA TCATCATCAT CATTGAGTTT
541 AAACGGTCTC CAGCTTGGCT GTTTTGGCGG ATGAGAGAAG ATTTTCAGCC TGATACAGAT
601 TAAATCAGAA CGCAGAAGCG GTCTGATAAA ACAGAATTTG CCTGGCGGCA GTAGCGCGGT
661 GGTCCCACCT GACCCCATGC CGAACTCAGA AGTGAAACGC CGTAGCGCCG ATGGTAGTGT
721 GGGGTCTCCC CATGCGAGAG TAGGGAACTG CCAGGCATCA AATAAAACGA AAGGCTCAGT
781 CGAAAGACTG GGCCTTTCGT TTTATCTGTT GTTTGTCGGT GAACGCTCTC CTGAGTAGGA
841 CAAATCCGCC GGGAGCGGAT TTGAACGTTG CGAAGCAACG GCCCGGAGGG TGGCGGGCAG
901 GACGCCCGCC ATAAACTGCC AGGCATCAAA TTAAGCAGAA GGCCATCCTG ACGGATGGCC
961 TTTTTGCGTT TCTACAAACT CTTTTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC
1021 GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG
1081 TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT
1141 TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT
1201 GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA
1261 ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT
1321 TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA
1381 GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG
1441 TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG
1501 ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG
1561 TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT
1621 AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG
1681 GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC
1741 CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG
1801 TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC
1861 GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT
1921 GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA
1981 ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA
2041 AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG
2101 ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC
2161 GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC
2221 TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA
2281 CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT
2341 GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC
2401 GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG
2461 AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC
2521 CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC
2581 GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT
2641 CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC
2701 CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT
2761 TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC
2821 CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG
2881 CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATGGTGCAC
2941 TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA
3001 CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG
3061 GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG
3121 TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCAGATCAA TTCGCGCGCG
3181 AAGGCGAAGC GGCATGCATT TACGTTGACA CCATCGAATG GTGCAAAACC TTTCGCGGTA
3241 TGGCATGATA GCGCCCGGAA GAGAGTCAAT TCAGGGTGGT GAATGTGAAA CCAGTAACGT
3301 TATACGATGT CGCAGAGTAT GCCGGTGTCT CTTATCAGAC CGTTTCCCGC GTGGTGAACC
3361 AGGCCAGCCA CGTTTCTGCG AAAACGCGGG AAAAAGTGGA AGCGGCGATG GCGGAGCTGA
3421 ATTACATTCC CAACCGCGTG GCACAACAAC TGGCGGGCAA ACAGTCGTTG CTGATTGGCG
3481 TTGCCACCTC CAGTCTGGCC CTGCACGCGC CGTCGCAAAT TGTCGCGGCG ATTAAATCTC
3541 GCGCCGATCA ACTGGGTGCC AGCGTGGTGG TGTCGATGGT AGAACGAAGC GGCGTCGAAG
3601 CCTGTAAAGC GGCGGTGCAC AATCTTCTCG CGCAACGCGT CAGTGGGCTG ATCATTAACT
3661 ATCCGCTGGA TGACCAGGAT GCCATTGCTG TGGAAGCTGC CTGCACTAAT GTTCCGGCGT
3721 TATTTCTTGA TGTCTCTGAC CAGACACCCA TCAACAGTAT TATTTTCTCC CATGAAGACG
3781 GTACGCGACT GGGCGTGGAG CATCTGGTCG CATTGGGTCA CCAGCAAATC GCGCTGTTAG
3841 CGGGCCCATT AAGTTCTGTC TCGGCGCGTC TGCGTCTGGC TGGCTGGCAT AAATATCTCA
3901 CTCGCAATCA AATTCAGCCG ATAGCGGAAC GGGAAGGCGA CTGGAGTGCC ATGTCCGGTT
3961 TTCAACAAAC CATGCAAATG CTGAATGAGG GCATCGTTCC CACTGCGATG CTGGTTGCCA
4021 ACGATCAGAT GGCGCTGGGC GCAATGCGCG CCATTACCGA GTCCGGGCTG CGCGTTGGTG
4081 CGGATATCTC GGTAGTGGGA TACGACGATA CCGAAGACAG CTCATGTTAT ATCCCGCCGT
4141 CAACCACCAT CAAACAGGAT TTTCGCCTGC TGGGGCAAAC CAGCGTGGAC CGCTTGCTGC
4201 AACTCTCTCA GGGCCAGGCG GTGAAGGGCA ATCAGCTGTT GCCCGTCTCA CTGGTGAAAA
4261 GAAAAACCAC CCTGGCGCCC AATACGCAAA CCGCCTCTCC CCGCGCGTTG GCCGATTCAT
4321 TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG CAACGCAATT
4381 AATGTGAGTT AGCGCGAATT GATCTG
//

pFLAG-CMV-3	pRB373
pET-28a-BFP-C	pATPase_TA
pBT-4	pProLabel-C
pLexm	pHSG422
pVPack-GP	pSC101-EcoRImeth+
pENTR11-Dual	pSH65
pBAD18	pIJ702-del35-55
pPIC ZαGB	pNEO-ars
pAGO	pET-21a(+)
pET-32 EK/LIC	pSUMO
pRSET-EGFP	pCRE-MetLuc2-Reporter
pDEST26	c-Flag pcDNA3
pOM2	pcDNA4/HisMax B
pcDNA3.1(+)/CAT	pGL4.41[luc2P/HSE/Hygro]
pCYM1	pML21
pGL4.38[luc2P/p53 RE/Hygro]	pTet-Splice
pWF1	pMal-c2G
pAN7-1	pNEO
pDC515	pREP4
pET-41a(+)	pAD-SV40T
pLLP-OmpA	pBD-NF-kB
pAcGFP1-N2	pdKeima-Red-S1
pHV23	pL-brevisGH3-MBP
pOrf681_TA	pDsRed-Monomer
Pgex-HRV3C	pEF1/V5-HisA
pcDNA3.1-GP130v1	pCGN978
pcDNA5/FRT	pUCTag
pSC101	pET-12c(+)
pUC4-KISS	pGL4.45[luc2P/ISRE/Hygro]
pIκB-EGFP	pACT-MyoD
pSET152	pCRE-DD-AmCyan1
pHY15	pMDLg/pRRE
EWD299	pLNCX2
pMTD1.3xA	pTi
pFLAG-CMV2	pcDNA6.2-DsRed2-MCS1 miR
pHS85	pCH2
pJSC73	pFA1
pSP72	pPIC9
pLVX-DD-ZsGreen1 Control	pPIC9k-His
pMT-Bip-V5-HisA	pMal-c4X
pBad43	pUG6
pBBR1MCS-2	pcDNA5/FRT/V5-His-TOPO
pHB-1	pBluescript II KS(-)
pTP-4	pCDH-CMV-MCS-EF1a-RFP+BSD
pET-32a(+)	pCas9D10A-GFP
pCL-Ampho	ISceI-GR-RFP
pCREj-2	pKLAC1
pGL4.72[hRlucCP]	pSUPER.neo+gfp
PX543	pALEX a,b,c
pIC20H	pEF6/myc-His B

pC003 - LshC2C2 locus into pACYC184 for	pAraGFPCDF
LB1366 (Bcl-2 promoter with MHS1234)	pEGFP-LC3
PB_tre_Cas9	pLentiV2-dCas9-VP64
Cbh_v5 AAV-CBE N-terminal	pCXLE-hOCT3/4
pEGFP-puro	EC2_10_dCas9_VPR_HC_sgRNA
pCDH-puro-Bcl-XL	pLJC5-3XHA-EGFP-PEX26
Lenti-luciferase-P2A-Neo	GST-HA-GFP11-N1
MSCV-(pBabe mcs)- human p210BCR-ABL-IRES	pJSC131 - Bacterial expression plasmid f
pMSCV-IRES-GFP II (pMIG II)	pGGA008
pAAV-EFS-CjCas9-eGFP-HIF1a	BPK3082
pM1s3AsR_TS-C7	pSBtet-GP
pNK9	pRAD1
1306 pSG5L HA FKHR wt	pM-Cas9
pCAG-GBP1-10gly-lexADBD	pRC-CMV-Rev1b
pSELECT-HA-mFOXO1	CKS2 gRNA (BRDN0001149143)
pFRT/TO/GFP-SCAF4 (CRISPR_resistant)	Tet-O-FUW-Neurod1
phage UbiC NLS HA stdMCP stdHalo	pLVX-EF1alpha-SARS-CoV-2-nsp13-2xStrep-I
pCS2TAL3-RR	pXAT2
pGL3 2 kb prom. CD274	pMXs-hOCT4 (Plath)
pNEU-hMbPylRS-4xU6M15	pET28a-At-TRL
pAc-Neo-OVA	EC1000
pGH335_MS2-AID*Δ-Hygro	pTRKH2
pMSCV-hygro-STING	AbVec1.1-IGKC
pHAGE-PTCH1	pCMV-hA3G-BE3
401 pSG5L	pAC1404-pCR8-mRuby2_NLSPUFa
FUmGW	pAAV-EF1a-FLEX-GTB
pT3-Neo-EF1a-GFP	pMonAID_nCas9-PmCDA_Hyg_ALS
VVT1	pBHt2
pSR47	c-myc-PT3EF1a
pTEF:ATP	pDIRECT_23D
pcDNA3.4_7C11-1 heavy chain	pICH86966::AtU6p::sgRNA_PDS
p201G Cas9	pCW-Cas9
pMal-T-Avi-His/BirA	pMJ329: pCMV-MmeFz2
3XMEF2-luc	pFC334
pCMV-MMLVgag-3xNES-ABE8e	pCAS9-mCherry empty
pAAV_hsyn_NES-his-CaMPARI2-F391W-WPRE-SV	pLenti-EML4-ALK variant 3a
EC2_3_dCas9_Mxi1_sgRNA	pLenti-DsRed_IRES_MAPT:EGFP
pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-	pNeae2
pAc-sgRNA-Cas9	800 pSG5L HA PTEN wt
TAL3393	PVALB mAb_BCN14.1.1A2_HC_Mouse IgG2a
p58sheLL	pCS2TAL3-DD
pNN9	ilux pGEX(-)
LeGO-G	pJKR-L-tetR
pAAV2/8	BECLIN-HA WT
pPPEM	pAAVS1-PC-GCaMP6f
pT4-CMV-GFP	pTRE Tight ArchT-GFP
pYES-mtGFP	pUCC001
pLVX-M-puro	pLenti X2 Puro DEST (w16-1)
pHR_Gal4UAS_humanTRAIL _IRES_mCherry_PGK	pQUAST>stop>mCD8-GFP
pAAVS1-PDi-CRISPRn	NM9-SpCas9-NLS3

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pTrcHis2 A

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