| 质粒名称: | pLVX-EF1alpha-2xStrep-SARS-CoV-2-orf7b-IRES-Puro |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 141389 |
| 存储实验室: | Nevan Krogan |
|
载体骨架基本信息 |
|
| 载体名称: | pLVX-EF1alpha-IRES-Puro |
| 载体出品公司: | Takara Bio |
| 空载体大小: | 8825 bp |
| 完整质粒大小: | 8825bp |
| 载体类型: | 哺乳细胞表达, Lentiviral |
| 筛选标记: | Puromycin |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 30 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | SARS-CoV-2-orf7b |
| 物种来源: | SARS-CoV-2 |
| 基因突变: | human codon optimized |
| 启动子: | EF1alpha |
| 标签/融合蛋白: | 2xStrep (N terminal on insert) |
| 克隆方法: | 未知 |
|
文献引用方法 |
|
| 材料和方法部分: | pLXV-EF1alpha-2xStrep-SARS-CoV-2-orf7b-IRES-Puro was a gift from Nevan Krogan (Addgene plasmid # 141389 ; http://n2t.net/addgene:141389 ; RRID:Addgene_141389) |
| 参考文献部分: | A SARS-CoV-2 protein interaction map reveals targets for drug repurposing. Gordon DE, Jang GM, Bouhaddou M, Xu J, Obernier K, White KM, O'Meara MJ, Rezelj VV, Guo JZ, Swaney DL, Tummino TA, Huttenhain R, Kaake RM, Richards AL, Tutuncuoglu B, Foussard H, Batra J, Haas K, Modak M, Kim M, Haas P, Polacco BJ, Braberg H, Fabius JM, Eckhardt M, Soucheray M, Bennett MJ, Cakir M, McGregor MJ, Li Q, Meyer B, Roesch F, Vallet T, Mac Kain A, Miorin L, Moreno E, Naing ZZC, Zhou Y, Peng S, Shi Y, Zhang Z, Shen W, Kirby IT, Melnyk JE, Chorba JS, Lou K, Dai SA, Barrio-Hernandez I, Memon D, Hernandez-Armenta C, Lyu J, Mathy CJP, Perica T, Pilla KB, Ganesan SJ, Saltzberg DJ, Rakesh R, Liu X, Rosenthal SB, Calviello L, Venkataramanan S, Liboy-Lugo J, Lin Y, Huang XP, Liu Y, Wankowicz SA, Bohn M, Safari M, Ugur FS, Koh C, Savar NS, Tran QD, Shengjuler D, Fletcher SJ, O'Neal MC, Cai Y, Chang JCJ, Broadhurst DJ, Klippsten S, Sharp PP, Wenzell NA, Kuzuoglu-Ozturk D, Wang HY, Trenker R, Young JM, Cavero DA, Hiatt J, Roth TL, Rathore U, Subramanian A, Noack J, Hubert M, Stroud RM, Frankel AD, Rosenberg OS, Verba KA, Agard DA, Ott M, Emerman M, Jura N, von Zastrow M, Verdin E, Ashworth A, Schwartz O, d'Enfert C, Mukherjee S, Jacobson M, Malik HS, Fujimori DG, Ideker T, Craik CS, Floor SN, Fraser JS, Gross JD, Sali A, Roth BL, Ruggero D, Taunton J, Kortemme T, Beltrao P, Vignuzzi M, Garcia-Sastre A, Shokat KM, Shoichet BK, Krogan NJ. Nature. 2020 Jul;583(7816):459-468. doi: 10.1038/s41586-020-2286-9. Epub 2020 Apr 30. 10.1038/s41586-020-2286-9 PubMed 32353859 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLXV-EF1alpha-2xStrep-SARS-CoV-2-orf7b-IRES-Puro 9036 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Lentiviral expression of SARS-CoV-2 protein; transient expression
and generate lentivirus.
ACCESSION .
VERSION .
KEYWORDS pLXV-EF1alpha-2xStrep-SARS-CoV-2-orf7b-IRES-Puro
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9036)
AUTHORS Gordon DE, et al.
TITLE A SARS-CoV-2-Human Protein-Protein Interaction Map Reveals Drug
Targets and Potential Drug Repurposing
JOURNAL BioRxiv March 2020
REFERENCE 2 (bases 1 to 9036)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..9036
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 1..634
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 681..806
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1303..1536
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1721..1765
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 2028..2143
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1 (lacking the first T)"
promoter 2338..3519
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 2568..3510
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 3467..3487
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
CDS 3553..3576
/codon_start=1
/product="peptide that binds Strep-Tactin(R), an engineered
form of streptavidin"
/label=Strep-Tag II
/translation="WSHPQFEK"
CDS 3613..3636
/codon_start=1
/product="peptide that binds Strep-Tactin(R), an engineered
form of streptavidin"
/label=Strep-Tag II
/translation="WSHPQFEK"
misc_feature 3786..4359
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(3953..3970)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 4180..4199
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 4392..4991
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(4392..4411)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 4888..4908
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
misc_feature 5005..5593
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(5058..5078)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(5476..5487)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 5800..6433
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
primer_bind complement(6561..6577)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6561..6577)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(6574..6596)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 6585..6601
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6609..6639)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6654..6675
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(6792..6809)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6963..7551)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(7043..7062)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7722..8582)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8345..8364
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8583..8687)
/gene="bla"
/label=AmpR promoter
polyA_signal 8735..8869
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(8772..8791)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 8826..8845
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg
2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc
2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct
2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gatctatttc cggtgaattc
3541 gccgccacca tgtggtccca tccgcagttt gagaagggtg gtggttcagg cggaggctcc
3601 gggggcggga gctggtctca cccgcaattt gaaaaaggcg ctgcggctat tgagctgtct
3661 ctcatcgatt tctatctctg tttcctcgcc ttccttcttt tcctggtctt gattatgctg
3721 ataattttct ggttcagtct cgaattgcaa gaccataacg agacatgcca cgcctaagga
3781 tcccgcccct ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc
3841 cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg
3901 gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc
3961 aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga
4021 agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg
4081 tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag
4141 tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc
4201 aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct
4261 cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac
4321 cacggggacg tggttttcct ttgaaaaaca cgatgataag cttgccacaa cccacaagga
4381 gacgaccttc catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
4441 cccgggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
4501 tcgacccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
4561 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
4621 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
4681 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
4741 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
4801 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
4861 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
4921 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
4981 ccggtgccta gacgcgtctg gaacaatcaa cctctggatt acaaaatttg tgaaagattg
5041 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
5101 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
5161 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
5221 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
5281 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
5341 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag
5401 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
5461 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
5521 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
5581 gccgcctccc cgcctggaat taattctgca gtcgagacct agaaaaacat ggagcaatca
5641 caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg
5701 aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag
5761 ctgtagatct tagccacttt ttaaaagaaa agaggggact ggaagggcta attcactccc
5821 aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
5881 agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca
5941 agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct
6001 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga
6061 ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca
6121 agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc
6181 gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt
6241 tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
6301 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
6361 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
6421 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
6481 aaatgaatat cagagagtga gaggccttga cattgctagc gtttaccgtc gacctctagc
6541 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
6601 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
6661 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
6721 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
6781 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
6841 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
6901 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
6961 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
7021 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
7081 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
7141 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
7201 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
7261 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
7321 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
7381 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
7441 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
7501 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
7561 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
7621 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
7681 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
7741 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
7801 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
7861 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
7921 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
7981 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
8041 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
8101 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
8161 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
8221 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
8281 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
8341 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
8401 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
8461 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
8521 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
8581 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
8641 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
8701 aaagtgccac ctgacgtcga cggatcggga gatcaacttg tttattgcag cttataatgg
8761 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
8821 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac
8881 tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta
8941 cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa
9001 ttgtatttgt taaatatgta ctacaaactt agtagt
//
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