| 质粒名称: | pBAV1k-T5-LUX |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 55800 |
| 存储实验室: | Ichiro Matsumura |
|
载体骨架基本信息 |
|
| 载体名称: | pBAV1k |
| 空载体大小: | 2709bp |
| 完整质粒大小: | 8599 bp |
| 载体类型: | Synthetic Biology细胞表达 |
| 载体抗性: | Kanamycin |
| 生长温度: | 30 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 低拷贝 |
|
插入基因信息 |
|
| 插入基因名称1: | luxA |
| 物种来源: | Photorhabdus luminescens |
| 插入大小: | 1082 bp |
| 启动子: | T5 |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | EcoRI(未破坏) |
| 3’克隆位点: | SpeI(未破坏) |
| 5’测序引物: | 未知 |
| 3’测序引物: | 未知 |
| 插入基因名称2: | luxB |
| 物种来源: | Photorhabdus luminescens |
| 插入大小: | 983bp |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | XbaI (destroyed during cloning) |
| 3’克隆位点: | SpeI (destroyed during cloning) |
| 5’测序引物: | 未知 |
| 3’测序引物: | 未知 |
| 插入基因名称3: | luxC |
| 物种来源: | Photorhabdus luminescens |
| 插入大小: | 1442bp |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | XbaI (destroyed during cloning) |
| 3’克隆位点: | SpeI (destroyed during cloning) |
| 5’测序引物: | 未知 |
| 3’测序引物: | 未知 |
| 插入基因名称4: | luxD |
| 物种来源: | Photorhabdus luminescens |
| 插入大小: | 923bp |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: |
XbaI (destroyed during cloning)
|
| 3’克隆位点: | PstI (not destroyed) |
| 5’测序引物: | 未知 |
| 3’测序引物: | 未知 |
|
文献引用方法 |
|
| 材料和方法部分: | pBAV1k-T5-LUX was a gift from Ichiro Matsumura (Addgene plasmid # 55800) |
| 参考文献部分: |
pBAV1k-T5-LUX was a gift from Ichiro Matsumura (Addgene plasmid # 55800)
For your References section:
Rational design of a plasmid origin that replicates efficiently in both gram-positive and gram-negative bacteria. Bryksin AV, Matsumura I. PLoS One. 2010 . 5(10):e13244. 10.1371/journal.pone.0013244 PubMed 20949038
|
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pBAV1k-T5-LUX 8599 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Expresses the Photorhabdus luminescens lux operon in bacterial
cells.
ACCESSION .
VERSION .
KEYWORDS pBAV1k-T5-LUX
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8599)
AUTHORS Bryksin AV, Matsumura I
TITLE Rational design of a plasmid origin that replicates efficiently in
both gram-positive and gram-negative bacteria.
JOURNAL PLoS One. 2010 . 5(10):e13244.
PUBMED 20949038
REFERENCE 2 (bases 1 to 8599)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..8599
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind 14..30
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 40..51
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
CDS 58..1140
/codon_start=1
/gene="luxA"
/product="LuxA luciferase subunit"
/label=LuxA
/note="from the Photorhabdus luminescens bioluminescence
operon"
/translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
KLFQSDVMPFLKEKQRSLLY"
RBS 1152..1163
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
CDS 1170..2153
/codon_start=1
/gene="luxB"
/product="LuxB luciferase subunit"
/label=LuxB
/note="from the Photorhabdus luminescens bioluminescence
operon"
/translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILG
FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
RBS 2165..2176
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
CDS 2183..3625
/codon_start=1
/gene="luxC"
/product="LuxC fatty acid reductase"
/label=LuxC
/note="from the Photorhabdus luminescens bioluminescence
operon"
/translation="MTKKISFIINGQVEIFPESDDLVQSINFGDNSVYLPILNDSHVKN
IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
MRHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
DVAVEIEQTRFLEEDKFLVFVP"
RBS 3637..3648
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
CDS 3655..4578
/codon_start=1
/gene="luxD"
/product="LuxD acyltransferase"
/label=LuxD
/note="from the Photorhabdus luminescens bioluminescence
operon"
/translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
PDNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
FEHLTIATVNERRMRIEIENQAISLS"
RBS 4590..4601
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
CDS 4608..5720
/codon_start=1
/gene="luxE"
/product="LuxE"
/label=LuxE
/note="from the Photorhabdus luminescens bioluminescence
operon"
/translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
misc_feature 5724..5744
/label=BioBrick suffix
/note="universal suffix for all parts"
terminator 5795..5881
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
CDS 7212..7223
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
terminator 7425..7519
/label=lambda t0 terminator
/note="transcription terminator from phage lambda"
CDS complement(7535..8329)
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin"
/translation="MAKMRISPELKKLIEEYRCVKDTEGMSPAKVYKLVGENEILYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEGYED
EQSPEKTIELYAECIRLFHSIDISDCPYTNSLDGRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
misc_feature 8535..8556
/label=BioBrick prefix
/note="BioBrick prefix for parts that do not start with
""ATG"""
promoter 8561..6
/label=T5 promoter
/note="bacteriophage T5 promoter for E. coli RNA
polymerase, with embedded lac operator"
protein_bind 8581..8597
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
1 ataatagatt caattgtgag cggataacaa ttactagaga aagaggagaa atactagatg
61 aaatttggaa actttttgct tacataccaa cctccccaat tttctcaaac agaggtaatg
121 aaacgtttgg ttaaattagg tcgcatctct gaggagtgtg gttttgatac cgtatggtta
181 ctggagcatc atttcacgga gtttggtttg cttggtaacc cttatgtcgc tgctgcatat
241 ttacttggcg cgactaaaaa attgaatgta ggaactgccg ctattgttct tcccacagcc
301 catccagtac gccaacttga agatgtgaat ttattggatc aaatgtcaaa aggacgattt
361 cggtttggta tttgccgagg gctttacaac aaggactttc gcgtattcgg cacagatatg
421 aataacagtc gcgccttagc ggaatgctgg tacgggctga taaagaatgg catgacagag
481 ggatatatgg aagctgataa tgaacatatc aagttccata aggtaaaagt aaaccccgcg
541 gcgtatagca gaggtggcgc accggtttat gtggtggctg aatcagcttc gacgactgag
601 tgggctgctc aatttggcct accgatgata ttaagttgga ttataaatac taacgaaaag
661 aaagcacaac ttgagcttta taatgaagtg gctcaagaat atgggcacga tattcataat
721 atcgaccatt gcttatcata tataacatct gtagatcatg actcaattaa agcgaaagag
781 atttgccgga aatttctggg gcattggtat gattcttatg tgaatgctac gactattttt
841 gatgattcag accaaacaag aggttatgat ttcaataaag ggcagtggcg tgactttgta
901 ttaaaaggac ataaagatac taatcgccgt attgattaca gttacgaaat caatcccgtg
961 ggaacgccgc aggaatgtat tgacataatt caaaaagaca ttgatgctac aggaatatca
1021 aatatttgtt gtggatttga agctaatgga acagtagacg aaattattgc ttccatgaag
1081 ctcttccagt ctgatgtcat gccatttctt aaagaaaaac aacgttcgct attatattaa
1141 taatactaga gaaagaggag aaatactaga tgaaatttgg attgttcttc cttaacttca
1201 tcaattcaac aactgttcaa gaacaaagta tagttcgcat gcaggaaata acggagtatg
1261 ttgataagtt gaattttgaa cagattttag tgtatgaaaa tcatttttca gataatggtg
1321 ttgtcggcgc tcctctgact gtttctggtt ttctgctcgg tttaacagag aaaattaaaa
1381 ttggttcatt aaatcacatc attacaactc atcatcctgt ccgcatagcg gaggaagctt
1441 gcttattgga tcagttaagt gaagggagat ttattttagg gtttagtgat tgcgaaaaaa
1501 aagatgaaat gcattttttt aatcgcccgg ttgaatatca acagcaacta tttgaagagt
1561 gttatgaaat cattaacgat gctttaacaa caggctattg taatccagat aacgattttt
1621 atagcttccc taaaatatct gtaaatcccc atgcttatac gccaggcgga cctcggaaat
1681 atgtaacagc aaccagtcat catattgttg agtgggcggc caaaaaaggt attcctctca
1741 tctttaagtg ggatgattct aatgatgtta gatatgaata tgctgaaaga tataaagccg
1801 ttgcggataa atatgacgtt gacctatcag agatagacca tcagttaatg atattagtta
1861 actataacga agatagtaat aaagctaaac aagagacgcg tgcatttatt agtgattatg
1921 ttcttgaaat gcaccctaat gaaaatttcg aaaataaact tgaagaaata attgcagaaa
1981 acgctgtcgg aaattatacg gagtgtataa ctgcggctaa gttggcaatt gaaaagtgtg
2041 gtgcgaaaag tgtattgctg tcctttgaac caatgaatga tttgatgagc caaaaaaatg
2101 taatcaatat tgttgatgat aatattaaga agtaccacat ggaatatacc taataatact
2161 agagaaagag gagaaatact agatgactaa aaaaatttca ttcattatta acggccaggt
2221 tgaaatcttt cccgaaagtg atgatttagt gcaatccatt aattttggtg ataatagtgt
2281 ttacctgcca atattgaatg actctcatgt aaaaaacatt attgattgta atggaaataa
2341 cgaattacgg ttgcataaca ttgtcaattt tctctatacg gtagggcaaa gatggaaaaa
2401 tgaagaatac tcaagacgca ggacatacat tcgtgactta aaaaaatata tgggatattc
2461 agaagaaatg gctaagctag aggccaattg gatatctatg attttatgtt ctaaaggcgg
2521 cctttatgat gttgtagaaa atgaacttgg ttctcgccat atcatggatg aatggctacc
2581 tcaggatgaa agttatgttc gggcttttcc gaaaggtaaa tctgtacatc tgttggcagg
2641 taatgttcca ttatctggga tcatgtctat attacgcgca attttaacta agaatcagtg
2701 tattataaaa acatcgtcaa ccgatccttt taccgctaat gcattagcgt taagttttat
2761 tgatgtagac cctaatcatc cgataacgcg ctctttatct gttatatatt ggccccacca
2821 aggtgataca tcactcgcaa aagaaattat gcgacatgcg gatgttattg tcgcttgggg
2881 agggccagat gcgattaatt gggcggtaga gcatgcgcca tcttatgctg atgtgattaa
2941 atttggttct aaaaagagtc tttgcattat cgataatcct gttgatttga cgtccgcagc
3001 gacaggtgcg gctcatgatg tttgttttta cgatcagcga gcttgttttt ctgcccaaaa
3061 catatattac atgggaaatc attatgagga atttaagtta gcgttgatag aaaaacttaa
3121 tctatatgcg catatattac cgaatgccaa aaaagatttt gatgaaaagg cggcctattc
3181 tttagttcaa aaagaaagct tgtttgctgg attaaaagta gaggtggata ttcatcaacg
3241 ttggatgatt attgagtcaa atgcaggtgt ggaatttaat caaccacttg gcagatgtgt
3301 gtaccttcat cacgtcgata atattgagca aatattgcct tatgttcaaa aaaataagac
3361 gcaaaccata tctatttttc cttgggagtc atcatttaaa tatcgagatg cgttagcatt
3421 aaaaggtgcg gaaaggattg tagaagcagg aatgaataac atatttcgag ttggtggatc
3481 tcatgacgga atgagaccgt tgcaacgatt agtgacatat atttctcatg aaaggccatc
3541 taactatacg gctaaggatg ttgcggttga aatagaacag actcgattcc tggaagaaga
3601 taagttcctt gtatttgtcc cataataata ctagagaaag aggagaaata ctagatggaa
3661 aatgaatcaa aatataaaac catcgaccac gttatttgtg ttgaaggaaa taaaaaaatt
3721 catgtttggg aaacgctgcc agaagaaaac agcccaaaga gaaagaatgc cattattatt
3781 gcgtctggtt ttgcccgcag gatggatcat tttgctggtc tggcggaata tttatcgcgg
3841 aatggatttc atgtgatccg ctatgattcg cttcaccacg ttggattgag ttcagggaca
3901 attgatgaat ttacaatgtc tataggaaag cagagcttgt tagcagtggt tgattggtta
3961 actacacgaa aaataaataa cttcggtatg ttggcttcaa gcttatctgc gcggatagct
4021 tatgcaagcc tatctgaaat caatgcttcg tttttaatca ccgcagtcgg tgttgttaac
4081 ttaagatatt ctcttgaaag agctttaggg tttgattatc tcagtctacc cattaatgaa
4141 ttgccggata atctggattt tgaaggccat aaattgggtg ctgaagtctt tgcgagagat
4201 tgtcttgatt ttggttggga agatttagct tctacaatta ataacatgat gtatcttgat
4261 ataccgttta ttgcttttac tgcaaataac gataattggg tcaagcaaga tgaagttatc
4321 acattgttat caaatattcg tagtaatcga tgcaagatat attctttgtt aggaagttcg
4381 catgacttga gtgaaaattt agtggtcctg cgcaattttt atcaatcggt tacgaaagcc
4441 gctatcgcga tggataatga tcatctggat attgatgttg atattactga accgtcattt
4501 gaacatttaa ctattgcgac agtcaatgaa cgccgaatga gaattgagat tgaaaatcaa
4561 gcaatttctc tgtcttaata atactagaga aagaggagaa atactagatg acttcatatg
4621 ttgataaaca agaaattaca gcaagctcag aaattgatga tttgattttt tcgagcgatc
4681 cattagtgtg gtcttacgac gagcaggaaa aaatcagaaa gaaacttgtg cttgatgcat
4741 ttcgtaatca ttataaacat tgtcgagaat atcgtcacta ctgtcaggca cacaaagtag
4801 atgacaatat tacggaaatt gatgacatac ctgtattccc aacatcggtt tttaagttta
4861 ctcgcttatt aacttctcag gaaaacgaga ttgaaagttg gtttaccagt agcggcacga
4921 atggtttaaa aagtcaggtg gcgcgtgaca gattaagtat tgagagactc ttaggctctg
4981 tgagttatgg catgaaatat gttggtagtt ggtttgatca tcaaatagaa ttagtcaatt
5041 tgggaccaga tagatttaat gctcataata tttggtttaa atatgttatg agtttggtgg
5101 aattgttata tcctacgaca tttaccgtaa cagaagaacg aatagatttt gttaaaacat
5161 tgaatagtct tgaacgaata aaaaatcaag ggaaagatct ttgtcttatt ggttcgccat
5221 actttattta tttactctgc cattatatga aagataaaaa aatctcattt tctggagata
5281 aaagccttta tatcataacc ggaggcggct ggaaaagtta cgaaaaagaa tctctgaaac
5341 gtgatgattt caatcatctt ttatttgata ctttcaatct cagtgatatt agtcagatcc
5401 gagatatatt taatcaagtt gaactcaaca cttgtttctt tgaggatgaa atgcagcgta
5461 aacatgttcc gccgtgggta tatgcgcgag cgcttgatcc tgaaacgttg aaacctgtac
5521 ctgatggaac gccggggttg atgagttata tggatgcgtc agcaaccagt tatccagcat
5581 ttattgttac cgatgatgtc gggataatta gcagagaata tggtaagtat cccggcgtgc
5641 tcgttgaaat tttacgtcgc gtcaatacga ggacgcagaa agggtgtgct ttaagcttaa
5701 ccgaagcgtt tgatagttaa taatactagt agcggccgct gcaggcctca gggcccgatc
5761 gatgccgccg cttaattaat taatccagag gcatcaaata aaacgaaagg ctcagtcgaa
5821 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa
5881 tccgccgccc tagacctagt gtcattttat ttcccccgtt tcagcatcaa gaacctttgc
5941 ataacttgct ctatatccac actgataatt gccctcaaac cataatctaa aggcgctaga
6001 gtttgttgaa acaatatctt ttacatcatt cgtatttaaa attccaaact ccgctcccct
6061 aaggcgaata aaagccatta aatcttttgt atttaccaaa ttatagtcat ccactatatc
6121 taagagtaaa ttcttcaatt ctcttttttg gctttcatca agtgttatat agcggtcaat
6181 atcaaaatca ttaatgttca aaatatcttt tttgtcgtat atatgtttat tcttagcaat
6241 agcgtccttt gattcatgag tcaaatattc atatgaacct ttgatataat caagtatctc
6301 aacatgagca actgaactat tccccaattt tcgcttaatc ttgttcctaa cgctttctat
6361 tgttacagga tttcgtgcaa tatatataac gtgatagtgt ggttttttat agtgctttcc
6421 atttcgtata acatcactac tattccatgt atctttatct tttttttcgt ccatatcgtg
6481 taaaggactg acagccatag atacgcccaa actctctaat ttttccttcc aatcattagg
6541 aattgagtca ggatataata aaaatccaaa atttctagct ttagtatttt taatagccat
6601 gatataatta ccttatcaaa aacaagtagc gaaaactcgt atccttctaa aaacgcgagc
6661 tttcgcttat tttttttgtt ctgattcctt tcttgcatat tcttctatag ctaacgccgc
6721 aaccgcagat tttgaaaaac ctttttgttt cgccatatct gttaattttt tatcttgctc
6781 ttttgtcaga gaaatcataa ctcttttttt cgattctgaa atcaccattt aaaaaactcc
6841 aatcaaataa ttttataaag ttagtgtatc actttgtaat cataaaaaca acaataaagc
6901 tacttaaata tagatttata aaaaacgttg gcgaaaacgt tggcgattcg ttggcgattg
6961 aaaaacccct taaacccttg agccagttgg gatagagcgt ttttggcaca aaaattggca
7021 ctcggcactt aatggggggt cgtagtacgg aagcaaaatt cgcttccttt ccccccattt
7081 ttttccaaat tccagatttt tttcaaaaat tttccagcgc taccgctcgg caaaattgca
7141 agcaattttt aaaatcaaac ccatgaggga atttcattcc ctcatactcc cttgagcctc
7201 ctccaaccga aatagaaggg cgctgcgctt attatttcat tcagtcatcg gctttcataa
7261 tctaacagac aacatcttcg ctgcaaagcc acgctacgct caagggcttt tacgctacga
7321 taacgcctgt tttaacgatt atgccgataa ctaaacgaaa taaacgctaa aacgtctcag
7381 aaacgatttt gagacgtttt aataaaaaat cgcctagtgc ttggattctc accaataaaa
7441 aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact
7501 ggatctatca acgggagtcc aagcgagctc ggtactaaaa caattcatcc agtaaaatat
7561 aatattttat tttctcccaa tcaggcttga tccccagtaa gtcaaaaaat agctcgacat
7621 actgttcttc cccgatatcc tccctgatcg accggacgca gaaggcaatg tcataccact
7681 tgtccgccct gccgcttctc ccaagatcaa taaagccact tactttgcca tctttcacaa
7741 agatgttgct gtctcccagg tcgccgtggg aaaagacaag ttcctcttcg ggcttttccg
7801 tctttaaaaa atcatacagc tcgcgcggat ctttaaatgg agtgtcttct tcccagtttt
7861 cgcaatccac atcggccaga tcgttattca gtaagtaatc caattcggct aagcggccgt
7921 ctaagctatt cgtataggga caatccgata tgtcgatgga gtgaaagagc ctgatgcact
7981 ccgcatacag ctcgatagtc ttttcagggc tttgttcatc ttcataccct tccgagcaaa
8041 ggacgccatc ggcctcactc atgagcagat tgctccagcc atcatgccgt tcaaagtgca
8101 ggacctttgg aacaggcagc tttccttcca gccatagcat catgtccttt tcccgttcca
8161 catcataggt ggtcccttta taccggctgt ccgtcatttt taaatatagg atttcatttt
8221 ctcccaccag cttatatacc ttagcaggag acattccttc cgtatctttt acgcagcggt
8281 attcttcgat cagttttttc aattccggtg atattctcat tttagccatt tattatttcc
8341 ttcctctttt ctacagtatt taaagatacc ccaagaagct aattataaca agacgaactc
8401 caattcactg ttccttgcat tctaaaacct taaatacaga aaacagcctt ttcaaagttg
8461 ttttcaaagt tggcgtataa catagtatcg acggagccga ttttgaaacc acaattatga
8521 tagaatttga cgtcgaattc gcggccgctt ctagaggaaa tcataaaaaa tttatttgct
8581 ttgtgagcgg ataacaatt
//
