| 质粒名称: | pSIP403 |
|---|---|
| 出品公司： | addgene |
| 目录编号: | 122028 |
| 存储实验室: | Lars Axelsson, Geir Mathiesen |
|
载体骨架基本信息 |
|
| 载体名称: | spp-based expression vector (pSIP401) |
| 空载体大小: | 5400 bp |
| 完整质粒大小: | 7435bp |
| 载体类型: | 细菌细胞表达 |
| 载体抗性: | Erythromycin |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | gusA |
| 插入大小: | 1809bp |
| 启动子: | sppA |
| 5’克隆位点: | NcoI (未破坏) |
| 3’克隆位点: | Multi (未破坏) |
| 5’测序引物 | GGCTTTTATAATATGAGATAATGCCGAC |
|
文献引用方法 |
|
| 材料和方法部分: | pSIP403 was a gift from Lars Axelsson & Geir Mathiesen (Addgene plasmid # 122028 ; http://n2t.net/addgene:122028 ; RRID:Addgene_122028) |
| 参考文献部分: | High-level, inducible gene expression in Lactobacillus sakei and Lactobacillus plantarum using versatile expression vectors. Sorvig E, Mathiesen G, Naterstad K, Eijsink VG, Axelsson L. Microbiology. 2005 Jul;151(Pt 7):2439-49. doi: 10.1099/mic.0.28084-0. 10.1099/mic.0.28084-0 PubMed 16000734 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pSIP403 7435 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Plasmid for expression of gene of interest in Lactobacillus
plantarum and L. sakei.
ACCESSION .
VERSION .
KEYWORDS pSIP403
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7435)
AUTHORS Sorvig E, Mathiesen G, Naterstad K, Eijsink VG, Axelsson L
TITLE High-level, inducible gene expression in Lactobacillus sakei and
Lactobacillus plantarum using versatile expression vectors.
JOURNAL Microbiology. 2005 Jul;151(Pt 7):2439-49. doi:
10.1099/mic.0.28084-0.
PUBMED 16000734
REFERENCE 2 (bases 1 to 7435)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7435
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 575..2383
/codon_start=1
/gene="uidA (or gusA) from E. coli"
/product="beta-glucuronidase"
/label=GUS
/translation="MVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
PKSAAFLLQKRWTGMNFGEKPQGGKQ"
rep_origin 2731..3319
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 3220..3239
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 tagttaacct atctaatatt gccaattatg acagtaaaac acggagttta aaatttgtag
61 atggcagtga ggcaaaagtc tcgttccgga aatcacggga actagtggcc aaattaaaac
121 aaatgatgta gcgcctgcag cacgccaaat gatcccagta aaaagccacc cgcatggcgg
181 gtggcttttt attagcccta gaagggcttc ccacacgcat ttcagcgcct tagtgcctta
241 gtttgtgaat cataggtggt atagtcccga aatacccgtc taaggaattg tcagataggc
301 ctaatgactg gcttttataa tatgagataa tgccgactgt actttttaca gtcggttttc
361 taatgtcact aacctgcccc gttagttgaa gaaggttttt atattacagc tccagatcta
421 ccggtgggcc catattaacg tttaaccgat aaagttgaac gttaatattt tttttgcgca
481 gaaatggtaa attgaagcat aatagtcttg taaggtattt agctggctgg cgtaaagtat
541 gctttataaa ataatatata ggagtatgat tcccatggta cgtcctgtag aaaccccaac
601 ccgtgaaatc aaaaaactcg acggcctgtg ggcattcagt ctggatcgcg aaaactgtgg
661 aattgatcag cgttggtggg aaagcgcgtt acaagaaagc cgggcaattg ctgtgccagg
721 cagttttaac gatcagttcg ccgatgcaga tattcgtaat tatgcgggca acgtctggta
781 tcagcgcgaa gtctttatac cgaaaggttg ggcaggccag cgtatcgtgc tgcgtttcga
841 tgcggtcact cattacggca aagtgtgggt caataatcag gaagtgatgg agcatcaggg
901 cggctatacg ccatttgaag ccgatgtcac gccgtatgtt attgccggga aaagtgtacg
961 tatcaccgtt tgtgtgaaca acgaactgaa ctggcagact atcccgccgg gaatggtgat
1021 taccgacgaa aacggcaaga aaaagcagtc ttacttccat gatttcttta actatgccgg
1081 aatccatcgc agcgtaatgc tctacaccac gccgaacacc tgggtggacg atatcaccgt
1141 ggtgacgcat gtcgcgcaag actgtaacca cgcgtctgtt gactggcagg tggtggccaa
1201 tggtgatgtc agcgttgaac tgcgtgatgc ggatcaacag gtggttgcaa ctggacaagg
1261 cactagcggg actttgcaag tggtgaatcc gcacctctgg caaccgggtg aaggttatct
1321 ctatgaactg tgcgtcacag ccaaaagcca gacagagtgt gatatctacc cgcttcgcgt
1381 cggcatccgg tcagtggcag tgaagggcga acagttcctg attaaccaca aaccgttcta
1441 ctttactggc tttggtcgtc atgaagatgc ggacttgcgt ggcaaaggat tcgataacgt
1501 gctgatggtg cacgaccacg cattaatgga ctggattggg gccaactcct accgtacctc
1561 gcattaccct tacgctgaag agatgctcga ctgggcagat gaacatggca tcgtggtgat
1621 tgatgaaact gctgctgtcg gctttaacct ctctttaggc attggtttcg aagcgggcaa
1681 caagccgaaa gaactgtaca gcgaagaggc agtcaacggg gaaactcagc aagcgcactt
1741 acaggcgatt aaagagctga tagcgcgtga caaaaaccac ccaagcgtgg tgatgtggag
1801 tattgccaac gaaccggata cccgtccgca aggtgcacgg gaatatttcg cgccactggc
1861 ggaagcaacg cgtaaactcg acccgacgcg tccgatcacc tgcgtcaatg taatgttctg
1921 cgacgctcac accgatacca tcagcgatct ctttgatgtg ctgtgcctga accgttatta
1981 cggatggtat gtccaaagcg gcgatttgga aacggcagag aaggtactgg aaaaagaact
2041 tctggcctgg caggagaaac tgcatcagcc gattatcatc accgaatacg gcgtggatac
2101 gttagccggg ctgcactcaa tgtacaccga catgtggagt gaagagtatc agtgtgcatg
2161 gctggatatg tatcaccgcg tctttgatcg cgtcagcgcc gtcgtcggtg aacaggtatg
2221 gaatttcgcc gattttgcga cctcgcaagg catattgcgc gttggcggta acaagaaagg
2281 gatcttcact cgcgaccgca aaccgaagtc ggcggctttt ctgctgcaaa aacgctggac
2341 tggcatgaac ttcggtgaaa aaccgcaggg aggcaaacaa tgatctagac tcgaggaatt
2401 cggtaccccg ggttcgaagg cgccaagctt caaattacag cacgtgttgc tttgattgat
2461 agccaaaaag cagcagttga taaagcaatt actgatattg ctgaaaaatt gtaatttata
2521 aataaaaatc accttttaga ggtggttttt ttatttataa attattcgtt tgatttcgct
2581 ttcgatagaa caatcaaagc gagaataagg aagataaatc ccataagggc gggagcagaa
2641 tgtccgagac taattcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
2701 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
2761 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
2821 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
2881 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
2941 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
3001 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
3061 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
3121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
3181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
3241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
3301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
3361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
3421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gctgcgagtc
3481 agtgagcgag aaggattatt cggctggttg agacgttaaa atgataaagg ttgtattaat
3541 cttatattac ggttataatg tactcaactt aataaatgaa cgcaaaaaaa agaaccctca
3601 acttagcaga gttaggattc acgacttatc agcacaacct gataagattt tcgatagcaa
3661 gtactaccaa tacaagctat ctaacttggt actattataa catgtaggct aagtttttca
3721 accattgata cttaaagtaa acggttgtta tcgggaatct taacagaaac ctgatagcaa
3781 ccgttttttt gttattcaat ggttagcaac catcaaagca actaaaggct ggaaacctgt
3841 tcttagctag taaaacctcc cgtgagtgtc gttcgtgacc ccgcttgcag ttaacaacat
3901 aggtatgcta aaccttgtcg agatcaacgc gactaaagac gtggctggaa gactaggaaa
3961 tgatacggac aggctaacta ttaacgcaga ttattcgggt tgctgctaaa accaactcta
4021 ataatagtta gtgcaagggc tggttgagct taaattgtct gataaagagt tctctcttta
4081 tactgcaaaa gaagcgcagt tattcacgat taggataact gtttgagaga gcctaagggc
4141 ttgacccttg atggtttaag caccgctatg cgtgcgggat ccccttagaa gcaaacttag
4201 agtgtgttga tagtgcatta tcttaaaatt ttgtataata ggaattgaag ttaaattaga
4261 tgctaaaaat aggaattgaa gttaaattag atgctaaaaa tttgtaatta agaaggaggg
4321 attcgtcatg ttggtattcc aaatgcgtaa tgtagataaa acatctactg ttttgaaaca
4381 gactaaaaac agtgattacg cagataaata aatacgttag attaattcct accagtgact
4441 aatcttatga ctttttaaac agataactaa aattacaaac aaatcgttta acttcaggag
4501 agattacatg aacaaaaata taaaatattc tcaaaacttt ttaacgagtg aaaaagtact
4561 caaccaaata ataaaacaat tgaatttaaa agaaaccgat accgtttacg aaattggaac
4621 aggtaaaggg catttaacga cgaaactggc taaaataagt aaacaggtaa cgtctattga
4681 attagacagt catctattca acttatcgtc agaaaaatta aaactgaata ctcgtgtcac
4741 tttaattcac caagatattc tacagtttca attccctaac aaacagaggt ataaaattgt
4801 tgggaatatt ccttacaatt taagcacaca aattattaaa aaagtggttt ttgaaagccg
4861 tgcgtctgac atctatctga ctgttgaaga aggattctac aagcgtacct tggatattca
4921 ccgaacacta gggttgctct tgcacactca agtctcgatt cagcaattgc ttaagctgcc
4981 agcggaatgc tttcatccta aaccaaaagt aaacagtgtc ttaataaaac ttacccgcca
5041 taccacagat gttccagata aatattggaa gctatataag tactttgttt caaaatgggt
5101 caatcgagaa tatcgtcaac tgtttactaa aaatcagttt cgtcaagcaa tgaaacacgc
5161 caaagtaaac aatttaagta ccattactta tgagcaagta ttgtctattt ttaatagtta
5221 tctattattt aacgggagga aataattcta tgagtcgctt ttttaaattt ggaaagttac
5281 acgttactaa agggaatgga gaccggggtc gacttcaata gagttcttaa cgttaatccg
5341 aaaaaaacta acgttaatat taaaaaataa gatccgcttg tgaattatgt ataatttgat
5401 tagactaaag aataggagaa agtatgatga tatttaaaaa actttctcgt taagataggt
5461 tgttggtgag catgttatat acggatgtat cggtttcctt aatgcaaaat tttgttgcta
5521 tcttattaat ttttctatta tatagatata ttcaaagaaa gataacattt aaacggatca
5581 tattagatat tttaatagcg attatttttt caatattata tctgtttatt tcagatgcgt
5641 cattacttgt aatggtatta atgcgattag ggtggcattt tcatcaacaa aaagaaaata
5701 agataaaaac gactgataca gctaatttaa ttctaattat cgtgatccag ttattgttag
5761 ttgcggttgg gactattatt agtcagttta ccatatcgat tatcaaaagt gatttcagcc
5821 aaaatatatt gaacaatagt gcaacagata taactttatt aggtattttc tttgctgttt
5881 tatttgacgg cttgttcttt atattattga agaataagcg gactgaatta caacatttaa
5941 atcaagaaat cattgaattt tcgttagaaa aacaatattt tatatttata tttattttat
6001 ttatagtaat agaaattatt ttagcagttg ggaatcttca aggagtaaca gccacgatat
6061 tattaaccat tatcattatt ttttgtgtcc ttatcgggat gactttttgg caagtgatgc
6121 tttttttgaa ggcttattcg attcgccaag aagccaatga ccaattggtc cggaatcaac
6181 aacttcaaga ttatctagtc aatatcgaac agcagtacac cgaattacgg cgatttaagc
6241 atgattatca aaacatctta ttatcgttgg agagttttgc cgaaaagggc gatcagcaac
6301 agtttaaggc gtattaccaa gaattattag cacaacggcc aattcaaagt gaaatccaag
6361 gggcagtcat tgcacaactc gactacttga aaaatgatcc tattcgagga ttagtcattc
6421 aaaagttttt ggcagccaaa caggctggtg ttactttaaa attcgaaatg accgaaccaa
6481 tcgaattagc aaccgctaat ctattaacgg ttattcggat tatcggtatt ttattagaca
6541 atgcgattga acaagccgtt caagaaaccg atcaattggt gagttgtgct ttcttacaat
6601 ctgatggttt aatcgaaatt acgattgaaa atacggccag tcaagttaag aatctccaag
6661 cattttcaga gttaggctat tcaacgaaag gcgctggtcg ggggactggt ttagctaatg
6721 tgcaggattt gattgccaaa caaaccaatt tattcttaga aacacagatt gaaaatagaa
6781 agttacgaca gacattgatg attacggagg aaacttaatt tgtatcccgt ttatttatta
6841 gaggatgatt tacagcaaca agcgatttat cagcaaatta tcgcgaatac gattatgatt
6901 aacgaatttg caatgacttt aacatgcgct gccagtgata ctgagacatt gttggcggca
6961 attaaggatc agcaacgagg tttattcttt ttggatatgg aaattgagga taaccgccaa
7021 gccggtttag aagtggcaac taagattcgg cagatgatgc cgtttgcgca aattgtcttc
7081 attacaaccc acgaggaact gacattatta acgttagaac gaaaaatagc gcctttagat
7141 tacattctca aggaccaaac aatggctgaa atcaaaaggc aattgattga tgatctattg
7201 ttagctgaga agcaaaacga ggcggcagcg tatcaccgag aaaatttatt tagttataaa
7261 ataggtcctc gctttttctc attaccatta aaggaagttg tttatttata tactgaaaaa
7321 gaaaatccgg gtcatattaa tttgttagcc gttaccagaa aggttacttt tccaggaaat
7381 ttaaatgcgc tggaagccca atatccaatg ctctttcggt gtgataaaag ttact
//
