| 质粒名称: | FHRE-Luc |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 1789 |
| 存储实验室: | Michael Greenberg |
|
载体骨架基本信息 |
|
| 载体名称: | pGL3 basic |
| 载体出品公司: | Promega |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 5000 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，荧光素酶 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | FHRE,FKHRL1,FOXO3a,forkhead responsive element |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 96 bp |
| 基因突变: | 3 copies of FRE |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | NheI(未破坏) |
| 3' 克隆位点: | BglII(未破坏) |
| 5' 测序引物 | RVprimer3 |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | FHRE-Luc was a gift from Michael Greenberg (Addgene plasmid # 1789 ; http://n2t.net/addgene:1789 ; RRID:Addgene_1789) |
| 参考文献部分: | Akt promotes cell survival by phosphorylating and inhibiting a Forkhead transcription factor. Brunet A, Bonni A, Zigmond MJ, Lin MZ, Juo P, Hu LS, Anderson MJ, Arden KC, Blenis J, Greenberg ME. Cell 1999 Mar 19;96(6):857-68. 10.1016/S0092-8674(00)80595-4 PubMed 10102273 |
买家导航
FHRELuc载体基本信息
FHRELuc载体图谱质粒图谱和多克隆位点信息
FHRELuc载体简介
FHRELuc载体序列
LOCUS FHRE-Luc 5094 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Reporter plasmid for FOXO3a. Forkhead responsive element with
luciferase readout. .
ACCESSION .
VERSION .
KEYWORDS FHRE-Luc
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5094)
AUTHORS Brunet A, Bonni A, Zigmond MJ, Lin MZ, Juo P, Hu LS, Anderson MJ,
Arden KC, Blenis J, Greenberg ME
TITLE Akt promotes cell survival by phosphorylating and inhibiting a
Forkhead transcription factor.
JOURNAL Cell 1999 Mar 19;96(6):857-68.
PUBMED 10102273
REFERENCE 2 (bases 1 to 5094)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5094
/organism="synthetic DNA construct"
/mol_type="other DNA"
polyA_signal 75..123
/note="synthetic polyadenylation signal"
misc_feature 137..228
/label=pause site
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
primer_bind 177..196
/label=RVprimer3
/note="pGL3 vector, forward primer"
promoter 367..563
/label=SV40 promoter
/note="SV40 early promoter"
rep_origin 414..549
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 476..495
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
regulatory 593..602
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 599..2251
/codon_start=1
/gene="luc+"
/product="firefly luciferase"
/label=luciferase
/note="enhanced luc+ version of the luciferase gene"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
primer_bind complement(665..683)
/label=LucNrev
/note="Firefly luciferase, reverse primer"
polyA_signal 2292..2413
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2303..2322)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 2357..2376
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind complement(2661..2678)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(2832..3420)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(2912..2931)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(3591..4451)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 4214..4233
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(4452..4556)
/gene="bla"
/label=AmpR promoter
rep_origin 4583..5038
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4670..4689)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 4880..4901
/label=F1ori-F
/note="F1 origin, forward primer"
ORIGIN
1 tcgctattac gccagcccaa gctaccatga taagtaagta atattaaggt acgggaggta
61 cttggagcgg ccgcaataaa atatctttat tttcattaca tctgtgtgtt ggttttttgt
121 gtgaatcgat agtactaaca tacgctctcc atcaaaacaa aacgaaacaa aacaaactag
181 caaaataggc tgtccccagt gcaagtgcag gtgccagaac atttctctat cgataggtac
241 cgagctctta cgcgtgctag cgtgacagag tgagactctg tctctattta aataaataag
301 taaataaata aactgggcaa acaatgaaaa tgaaaacatt gtgaaataca aagcagatct
361 gcgatctgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
421 ccctaactcc gcccagttcc gcccattctc cgccccatcg ctgactaatt ttttttattt
481 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
541 ttggaggcct aggcttttgc aaaaagcttg gcattccggt actgttggta aagccaccat
601 ggaagacgcc aaaaacataa agaaaggccc ggcgccattc tatccgctgg aagatggaac
661 cgctggagag caactgcata aggctatgaa gagatacgcc ctggttcctg gaacaattgc
721 ttttacagat gcacatatcg aggtggacat cacttacgct gagtacttcg aaatgtccgt
781 tcggttggca gaagctatga aacgatatgg gctgaataca aatcacagaa tcgtcgtatg
841 cagtgaaaac tctcttcaat tctttatgcc ggtgttgggc gcgttattta tcggagttgc
901 agttgcgccc gcgaacgaca tttataatga acgtgaattg ctcaacagta tgggcatttc
961 gcagcctacc gtggtgttcg tttccaaaaa ggggttgcaa aaaattttga acgtgcaaaa
1021 aaagctccca atcatccaaa aaattattat catggattct aaaacggatt accagggatt
1081 tcagtcgatg tacacgttcg tcacatctca tctacctccc ggttttaatg aatacgattt
1141 tgtgccagag tccttcgata gggacaagac aattgcactg atcatgaact cctctggatc
1201 tactggtctg cctaaaggtg tcgctctgcc tcatagaact gcctgcgtga gattctcgca
1261 tgccagagat cctatttttg gcaatcaaat cattccggat actgcgattt taagtgttgt
1321 tccattccat cacggttttg gaatgtttac tacactcgga tatttgatat gtggatttcg
1381 agtcgtctta atgtatagat ttgaagaaga gctgtttctg aggagccttc aggattacaa
1441 gattcaaagt gcgctgctgg tgccaaccct attctccttc ttcgccaaaa gcactctgat
1501 tgacaaatac gatttatcta atttacacga aattgcttct ggtggcgctc ccctctctaa
1561 ggaagtcggg gaagcggttg ccaagaggtt ccatctgcca ggtatcaggc aaggatatgg
1621 gctcactgag actacatcag ctattctgat tacacccgag ggggatgata aaccgggcgc
1681 ggtcggtaaa gttgttccat tttttgaagc gaaggttgtg gatctggata ccgggaaaac
1741 gctgggcgtt aatcaaagag gcgaactgtg tgtgagaggt cctatgatta tgtccggtta
1801 tgtaaacaat ccggaagcga ccaacgcctt gattgacaag gatggatggc tacattctgg
1861 agacatagct tactgggacg aagacgaaca cttcttcatc gttgaccgcc tgaagtctct
1921 gattaagtac aaaggctatc aggtggctcc cgctgaattg gaatccatct tgctccaaca
1981 ccccaacatc ttcgacgcag gtgtcgcagg tcttcccgac gatgacgccg gtgaacttcc
2041 cgccgccgtt gttgttttgg agcacggaaa gacgatgacg gaaaaagaga tcgtggatta
2101 cgtcgccagt caagtaacaa ccgcgaaaaa gttgcgcgga ggagttgtgt ttgtggacga
2161 agtaccgaaa ggtcttaccg gaaaactcga cgcaagaaaa atcagagaga tcctcataaa
2221 ggccaagaag ggcggaaaga tcgccgtgta attctagagt cggggcggcc ggccgcttcg
2281 agcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
2341 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
2401 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
2461 gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcg ataaggatcc
2521 gtcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca
2581 tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc
2641 cggcagcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
2701 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
2761 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
2821 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
2881 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
2941 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
3001 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
3061 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
3121 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
3181 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
3241 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
3301 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
3361 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
3421 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
3481 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
3541 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
3601 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
3661 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
3721 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
3781 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
3841 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
3901 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
3961 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
4021 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
4081 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
4141 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
4201 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
4261 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
4321 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
4381 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
4441 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
4501 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
4561 tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
4621 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
4681 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
4741 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
4801 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
4861 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
4921 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
4981 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttgc
5041 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctct
//
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