出品公司: | -- |
---|---|
载体名称: | pWPXL |
质粒类型: | 哺乳动物细胞慢病毒表达载体 |
高拷贝/低拷贝: | 高拷贝 |
启动子: | EF-1 alpha |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 10510 bp |
5' 测序引物及序列: | EF-1a Forward: TCAAGCCTCAGACAGTGGTTC |
3' 测序引物及序列: | EGFP-N: CGTCGCCGTCCAGCTCGACCAG |
载体标签: | C-EGFP |
载体抗性: | 氨苄 |
备注: | pWPXL载体是用来进行稳定型转基因表达。pWPXL载体含有EF-1alpha启动子和内含子,这使得基因能够高效表达。载体3‘ LTR区的LoxP被克隆至目的细胞的5’ LTR反转录区。这 使得之前在Cre酶作用下插入的基因得以移除出去。载体使用Stbl3感受态细胞扩增质粒,可以减少载体基因重组。 |
产品目录号: | -- |
稳定性: | / |
组成型: | -- |
病毒/非病毒: | 慢病毒 |
买家导航
pWPXL载体质粒基本信息
pWPXL质粒图谱载体图谱和pWPXL载体序列质粒序列多克隆位点信息
pWPXL质粒载体简介
pWPXL质粒序列载体序列
LOCUS pWPXL 10510 bp DNA circular SYN DEFINITION pWPXL ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. COMMENT This file is created by Vector NTI http://www.biofeng.com/ COMMENT VNTAUTHORNAME|biofeng.com| FEATURES Location/Qualifiers source 1..10510 /organism="pWPXL" /mol_type="other DNA" misc_feature 455..635 /label="truncHIV-1_3_LTR" misc_feature 455..635 /label="HIV-1_5_LTR" misc_feature 746..790 /label="HIV-1_psi_pack" CDS 1178..2035 /label="ORF frame 2" misc_feature 1300..1533 /label="RRE" CDS complement(1997..3037) /label="ORF frame 1" promoter 2127..3297 /label="EF1a_promoter" misc_feature 3245..3265 /label="EF1a_fwd_primer" misc_feature 3349..3364 /label="cPPT" CDS 3544..4281 /label="ORF frame 1" gene 3547..4260 /label="EGFP" /gene="EGFP" misc_feature complement(3589..3610) /label="EGFP_N_primer" misc_feature 3727..3756 /label="Y66 (EGFP)" misc_feature 4197..4218 /label="EGFP_C_primer" misc_feature 4342..4929 /label="WPRE" CDS complement(4427..4930) /label="ORF frame 1" CDS 4430..4990 /label="ORF frame 2" misc_feature 5000..5015 /label="cPPT" misc_feature 5000..5021 /label="U3PPT" misc_feature 5072..5105 /label="loxP" misc_feature 5125..5305 /label="truncHIV-1_3_LTR" misc_feature 5125..5305 /label="HIV-1_5_LTR" misc_feature complement(5331..5348) /label="Sp6_primer" gene 5586..5849 /label="tet (300 - 563)" /gene="tet (300 - 563)" misc_feature complement(5638..5657) /label="pBRrevBam_primer" misc_feature 5990..6012 /label="pGEX_3_primer" promoter 6171..6199 /label="AmpR_promoter" gene 6241..7101 /label="Ampicillin" /gene="Ampicillin" CDS 6241..7101 /label="ORF frame 1" rep_origin 7256..7875 /label="pBR322_origin" misc_feature complement(8101..8121) /label="pBABE_3_primer" misc_feature complement(8107..8560) /label="SV40_enhancer" promoter 8119..8387 /label="SV40_promoter" rep_origin 8286..8363 /label="SV40_origin" misc_feature 8348..8367 /label="SV40pro_F_primer" misc_feature 9568..9583 /label="SV40_int" misc_feature 9589..9636 /label="SV40_3_splice" CDS 9713..10186 /label="ORF frame 2" misc_feature 9764..9784 /label="SV40 NLS" misc_feature 9770..9784 /label="NLS" terminator 10212..10343 /label="SV40_PA_terminator" misc_feature 10300..10319 /label="EBV_rev_primer" ORIGIN 1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac 61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca 121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc 181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg 241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga 301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc 361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga 421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg 481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc 541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct 601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa 661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg 721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag 781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg 841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg 901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc 961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga 1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac 1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag 1141 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga 1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa 1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt 1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag 1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca 1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc 1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact 1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat 1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat 1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga 1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat 1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact 1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc 1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag 1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgatgtcgac gataagcttt 2041 gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt 2101 cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac 2161 agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg 2221 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg 2281 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc 2341 cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg 2401 cccttgcgtg ccttgaatta cttccactgg ctgcagtacg tgattcttga tcccgagctt 2461 cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt 2521 gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt 2581 cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct 2641 gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt 2701 atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg 2761 gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc 2821 cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg 2881 ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca 2941 gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa 3001 aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg 3061 ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg 3121 gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca 3181 gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc 3241 attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgagga 3301 atttcgacat ttaaatttaa ttaatctcga cggtatcggt taacttttaa aagaaaaggg 3361 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa 3421 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttccgatcac gagactagcc 3481 tcgaggttta aactacggga tccaggccta agcttacgcg tcctagcgct accggtcgcc 3541 accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg 3601 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc 3661 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc 3721 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg 3781 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc 3841 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc 3901 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg 3961 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag 4021 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc 4081 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac 4141 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg 4201 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag 4261 tccggactca gatctcgata gctagtagct agctagctag tcgagctcaa cttcgaattc 4321 ccgggactag tcatatgata atcaacctct ggattacaaa atttgtgaaa gattgactgg 4381 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta 4441 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct 4501 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt 4561 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac 4621 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg 4681 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac 4741 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg 4801 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct 4861 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc 4921 ctccccgcat cggtacgtat ggccaggtac ctttaagacc aatgacttac aaggcagctg 4981 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac 5041 gaagacaaga tgggatcaat tcaccatggg aataacttcg tatagcatac attatacgaa 5101 gttatgctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag 5161 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt 5221 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt 5281 tagtcagtgt ggaaaatctc tagcagcatc tagaattaat tccgtgtatt ctatagtgtc 5341 acctaaatcg tatgtgtatg atacataagg ttatgtatta attgtagccg cgttctaacg 5401 acaatatgta caagcctaat tgtgtagcat ctggcttact gaagcagacc ctatcatctc 5461 tctcgtaaac tgccgtcaga gtcggtttgg ttggacgaac cttctgagtt tctggtaacg 5521 ccgtcccgca cccggaaatg gtcagcgaac caatcagcag ggtcatcgct agccagatcc 5581 tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct 5641 atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt 5701 gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct 5761 tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct 5821 tcctaatgca ggagtcgcat aagggagagc gtcgaatggt gcactctcag tacaatctgc 5881 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga 5941 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc 6001 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata 6061 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact 6121 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg 6181 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt 6241 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct 6301 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca 6361 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc 6421 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc 6481 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg 6541 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta 6601 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc 6661 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt 6721 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg 6781 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct 6841 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc 6901 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct 6961 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac 7021 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc 7081 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat 7141 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg 7201 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc 7261 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa 7321 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag 7381 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta 7441 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta 7501 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag 7561 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg 7621 gagcgaacga cctacaccga actgagatac ctacagcgtg agcattgaga aagcgccacg 7681 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag 7741 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc 7801 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa 7861 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg 7921 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct 7981 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa 8041 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgt 8101 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc 8161 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag 8221 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc 8281 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa 8341 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt 8401 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct tggacacaag acaggcttgc 8461 gagatatgtt tgagaatacc actttatccc gcgtcaggga gaggcagtgc gtaaaaagac 8521 gcggactcat gtgaaatact ggtttttagt gcgccagatc tctataatct cgcgcaacct 8581 attttcccct cgaacacttt ttaagccgta gataaacagg ctgggacact tcacatgagc 8641 gaaaaataca tcgtcacctg ggacatgttg cagatccatg cacgtaaact cgcaagccga 8701 ctgatgcctt ctgaacaatg gaaaggcatt attgccgtaa gccgtggcgg tctgtaccgg 8761 gtgcgttact ggcgcgtgaa ctgggtattc gtcatgtcga taccgtttgt atttccagct 8821 acgatcacga caaccagcgc gagcttaaag tgctgaaacg cgcagaaggc gatggcgaag 8881 gcttcatcgt tattgatgac ctggtggata ccggtggtac tgcggttgcg attcgtgaaa 8941 tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc ggctggtcgt ccgctggttg 9001 atgactatgt tgttgatatc ccgcaagata cctggattga acagccgtgg gatatgggcg 9061 tcgtattcgt cccgccaatc tccggtcgct aatcttttca acgcctggca ctgccgggcg 9121 ttgttctttt taacttcagg cgggttacaa tagtttccag taagtattct ggaggctgca 9181 tccatgacac aggcaaacct gagcgaaacc ctgttcaaac cccgctttaa acatcctgaa 9241 acctcgacgc tagtccgccg ctttaatcac ggcgcacaac cgcctgtgca gtcggccctt 9301 gatggtaaaa ccatccctca ctggtatcgc atgattaacc gtctgatgtg gatctggcgc 9361 ggcattgacc cacgcgaaat cctcgacgtc caggcacgta ttgtgatgag cgatgccgaa 9421 cgtaccgacg atgatttata cgatacggtg attggctacc gtggcggcaa ctggatttat 9481 gagtgggccc cggatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa 9541 actacctaca gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt 9601 taaactactg attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg 9661 ggagcagtgg tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc 9721 tagtgatgat gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa 9781 ggtagaagac cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt 9841 tagtaataga actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct 9901 atacaagaaa attatggaaa aatattctgt aacctttata agtaggcata acagttataa 9961 tcataacata ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta 10021 tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt 10081 gatgtatagt gccttgacta gagatcataa tcagccatac cacatttgta gaggttttac 10141 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg 10201 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa 10261 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca 10321 atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac 10381 ttcccacccc ataccctatt accactgcca attacctagt ggtttcattt actctaaacc 10441 tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca 10501 aacttagtag //
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