出品公司: | Clontech |
---|---|
载体名称: | pLVX-PAmCherry-N1 |
质粒类型: | 慢病毒载体;哺乳动物细胞表达载体;荧光报告载体 |
高拷贝/低拷贝: | 高拷贝 |
启动子: | CMV |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 8777 bp |
5' 测序引物及序列: | CMV-F: CGCAAATGGGCGGTAGGCGTG (Invitrogen) |
3' 测序引物及序列: | -- |
载体标签: | PAmCherry(C-端) |
载体抗性: | 氨苄青霉素 |
筛选标记: | 嘌呤霉素(Puromycin) |
克隆菌株: | Stbl3 |
备注: |
pLVX-PAmCherry-N1载体表达的目的蛋白C端融PAmCherry荧光蛋白; CMV启动子驱动目的蛋白的过表达; 可用流式细胞仪筛选。 |
产品目录号: | 632586 |
稳定性: | 稳表达 |
组成型: | 组成型 |
病毒/非病毒: | 慢病毒 |
买家导航
pLVX-PAmCherry-N1载体质粒基本信息
pLVX-PAmCherry-N1质粒图谱载体图谱和pLVX-PAmCherry-N1载体序列质粒序列多克隆位点信息
pLVX-PAmCherry-N1质粒载体简介
pLVX-PAmCherry-N1质粒序列载体序列
LOCUS pLVX-PAmCherry-N1 8777 bp DNA circular SYN 8-AUG-2011 COMMENT Created by Clontech Laboratories Inc. COMMENT Cat. No.632586 COMMENT This file is created by Vector NTI http://www.biofeng.com/ COMMENT VNTAUTHORNAME|biofeng.com| FEATURES Location/Qualifiers source 1..8777 /organism="recombinant plasmid" /mol_type="other DNA" LTR 1..635 /label=5'\LTR /note="color: #000080" misc_feature 685..822 /label=Packaging\signal /note="color: #66cc99" misc_binding 1303..1536 /label=RRE /note="color: #009966" misc_feature 2796..2862 /label=MCS /note="color: #3366cc" CDS 2874..3581 /label=PAmCherry /note="color: #ff9966" promoter 3603..4111 /label=PGK\promoter /note="color: #66cc99" CDS 4132..4731 /label=Puro(R) /note="color: #ff9966" misc_feature 4745..5336 /label=WPRE /note="color: #66cc99" LTR 5539..6175 /label=3'\LTR /note="color: #000080" CDS complement(7463..8459) /label=Amp(R) /note="color: #ff9966" misc_feature 2028..2151 /label=CTS/cPPT\(DNA\flap) promoter 2185..2788 /label=CMV rep_origin complement(6645..7318) /label=pUC\ori BASE COUNT 2180 a 2267 c 2332 g 1998 t ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattctgca gtcgacggta ccgcgggccc gggatccgcc accatggtga 2881 gcaagggcga ggaggataac atggccatca ttaaggagtt catgcgcttc aaggtgcaca 2941 tggaggggtc cgtgaacggc cacgtgttcg agatcgaggg cgagggcgag ggccgcccct 3001 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcacct 3061 gggacatcct gtcccctcaa ttcatgtacg gctccaatgc ctacgtgaag caccccgccg 3121 acatccccga ctactttaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga 3181 aattcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggtgagt 3241 tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga 3301 agaagaccat gggctgggag gccctctccg agcggatgta ccccgaggac ggcgccctga 3361 agggcgaggt caagccgaga gtgaagctga aggacggcgg ccactacgac gctgaggtca 3421 agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaaccgca 3481 agttggacat cacctcacac aacgaggact acaccatcgt ggaacagtac gaacgtgccg 3541 agggccgcca ctccaccggc ggcatggacg agctgtacaa gtaagcggcc gcgactctag 3601 ataattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc 3661 agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc 3721 caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc 3781 tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat 3841 ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg 3901 ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag 3961 gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc 4021 ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct 4081 gttctcctct tcctcatctc cgggcctttc gacctgcagc ccaagcttac catgaccgag 4141 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc 4201 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc 4261 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag 4321 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa 4381 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg 4441 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg 4501 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc 4561 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc 4621 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag 4681 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg accgcgtctg 4741 gaacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt 4801 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc 4861 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga 4921 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc 4981 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct 5041 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg 5101 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct 5161 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc 5221 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg 5281 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat 5341 taattctgca gtcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct 5401 accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc 5461 acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt 5521 ttaaaagaaa agaggggact ggaagggcta attcactccc aacgaagaca agatatcctt 5581 gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg 5641 ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca 5701 gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg 5761 catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca 5821 tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct 5881 tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg 5941 gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct 6001 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 6061 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 6121 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag 6181 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga 6241 gaggccttga cattgctagc gttttaccgt cgacctctag ctagagcttg gcgtaatcat 6301 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag 6361 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg 6421 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa 6481 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca 6541 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 6601 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 6661 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 6721 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 6781 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 6841 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 6901 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 6961 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 7021 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 7081 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 7141 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 7201 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 7261 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 7321 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 7381 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 7441 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 7501 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 7561 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 7621 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 7681 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 7741 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct 7801 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 7861 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 7921 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 7981 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 8041 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac 8101 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 8161 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 8221 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 8281 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 8341 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 8401 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg 8461 acggatcggg agatcaactt gtttattgca gcttataatg gttacaaata aagcaatagc 8521 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 8581 ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat 8641 cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc 8701 taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt 8761 actacaaact tagtagt //
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