出品公司: | Clontech |
---|---|
载体名称: | pLVX-PAmCherry-C1 |
质粒类型: | 慢病毒载体;哺乳动物表达载体;荧光报告载体 |
高拷贝/低拷贝: | 高拷贝 |
启动子: | CMV |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 8770 bp |
5' 测序引物及序列: | CMV-F: CGCAAATGGGCGGTAGGCGTG (Invitrogen) |
3' 测序引物及序列: | -- |
载体标签: | PAmCherry(N-端) |
载体抗性: | 氨苄青霉素 |
筛选标记: | 嘌呤霉素(Puromycin) |
克隆菌株: | Stbl3 |
备注: |
pLVX-PAmCherry-C1载体表达的目的蛋白N端融PAmCherry荧光蛋白; CMV启动子驱动目的蛋白的过表达; 可用流式细胞仪筛选。 |
产品目录号: | 632587 |
稳定性: | 稳表达 |
组成型: | 组成型 |
病毒/非病毒: | 慢病毒 |
买家导航
pLVX-PAmCherry-C1载体质粒基本信息
pLVX-PAmCherry-C1质粒图谱载体图谱和pLVX-PAmCherry-C1载体序列质粒序列多克隆位点信息
pLVX-PAmCherry-C1质粒载体简介
pLVX-PAmCherry-C1质粒序列载体序列
LOCUS pLVX-PAmCherry-C1 8770 bp DNA circular SYN 8-AUG-2011 COMMENT Created by Clontech Laboratories Inc. COMMENT Cat. No.632587 COMMENT This file is created by Vector NTI http://www.biofeng.com/ COMMENT VNTAUTHORNAME|biofeng.com| FEATURES Location/Qualifiers source 1..8770 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..635 /label=5'\LTR /note="color: #000080" misc_feature 685..822 /label=Packaging\signal /note="color: #66cc99" misc_binding 1303..1536 /label=RRE /note="color: #009966" misc_feature 2028..2151 /label=CTS/cPPT\(DNA\flap) /note="color: #66cc99" promoter 2185..2788 /label=CMV /note="color: #66cc99" CDS 2806..3513 /label=PAmCherry /note="color: #ff9966" misc_feature 3530..3594 /label=MCS /note="color: #66cc99" promoter 3596..4104 /label=PGK\promoter /note="color: #66cc99" CDS 4125..4724 /label=Puro(R) /note="color: #66cc99" misc_feature 4738..5329 /label=WPRE /note="color: #66cc99" LTR 5532..6168 /label=3'\LTR /note="color: #000080" rep_origin complement(6638..7311) /label=pUC\ori /note="color: #ff9966" CDS complement(7456..8452) /label=Amp(R) /note="color: #ff9966" BASE COUNT 2178 a 2265 c 2330 g 1997 t ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tagctagagg atcgctagcg ccaccatggt gagcaagggc 2821 gaggaggata acatggccat cattaaggag ttcatgcgct tcaaggtgca catggagggg 2881 tccgtgaacg gccacgtgtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc 2941 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcac ctgggacatc 3001 ctgtcccctc aattcatgta cggctccaat gcctacgtga agcaccccgc cgacatcccc 3061 gactacttta agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaaattcgag 3121 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggtga gttcatctac 3181 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc 3241 atgggctggg aggccctctc cgagcggatg taccccgagg acggcgccct gaagggcgag 3301 gtcaagccga gagtgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc 3361 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaaccg caagttggac 3421 atcacctcac acaacgagga ctacaccatc gtggaacagt acgaacgtgc cgagggccgc 3481 cactccaccg gcggcatgga cgagctgtac aaggctaccg gactcagatc tcgagctcaa 3541 gcttcgaatt ctgcagtcga cggtaccgcg ggcccgggat cccgcgactc tagataattc 3601 taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg 3661 ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt 3721 aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta 3781 gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta 3841 gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc 3901 ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga 3961 aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg 4021 tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc 4081 tcttcctcat ctccgggcct ttcgacctgc agcccaagct taccatgacc gagtacaagc 4141 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg 4201 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg 4261 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg 4321 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg 4381 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc 4441 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg 4501 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc 4561 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc 4621 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg 4681 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaccgcgt ctggaacaat 4741 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct 4801 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg 4861 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg 4921 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt 4981 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt 5041 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg 5101 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc 5161 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat 5221 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc 5281 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattaattct 5341 gcagtcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg 5401 ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc 5461 aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag 5521 aaaagagggg actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt 5581 ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg 5641 tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg 5701 tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga 5761 tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc 5821 acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca 5881 agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc 5941 gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta 6001 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa 6061 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac 6121 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt 6181 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggcct 6241 tgacattgct agcgttttac cgtcgacctc tagctagagc ttggcgtaat catggtcata 6301 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag 6361 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg 6421 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca 6481 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc 6541 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 6601 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 6661 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 6721 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 6781 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 6841 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 6901 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 6961 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 7021 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 7081 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac 7141 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 7201 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 7261 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 7321 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 7381 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 7441 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 7501 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 7561 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 7621 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 7681 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 7741 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 7801 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 7861 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 7921 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 7981 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 8041 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 8101 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 8161 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 8221 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 8281 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 8341 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 8401 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc 8461 gggagatcaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa 8521 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca 8581 atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac 8641 ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct 8701 gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa 8761 acttagtagt //
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