| 出品公司: | Clontech |
|---|---|
| 载体名称: | pLVX-ZsGreen1-C1 |
| 质粒类型: | 慢病毒载体；哺乳动物细胞表达载体；荧光报告载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 启动子: | CMV |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 8763 bp |
| 5' 测序引物及序列: | CMV-F: CGCAAATGGGCGGTAGGCGTG (Invitrogen) |
| 3' 测序引物及序列: | -- |
| 载体标签: | ZsGreen1（N-端） |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 嘌呤霉素(Puromycin) |
| 克隆菌株: | Stbl3 |
| 备注: |
pLVX-ZsGreen1-C1载体表达的目的蛋白N端融ZsGreen1荧光蛋白； CMV启动子驱动目的蛋白的过表达； ZsGreen1是第三代绿色荧光蛋白，亮度极高，可用流式细胞仪筛选。 |
| 产品目录号: | 632566 |
| 稳定性: | 稳表达 |
| 组成型: | 组成型 |
| 病毒/非病毒: | 慢病毒 |
买家导航
pLVX-ZsGreen1-C1载体质粒基本信息
pLVX-ZsGreen1-C1质粒图谱载体图谱和pLVX-ZsGreen1-C1载体序列质粒序列多克隆位点信息
pLVX-ZsGreen1-C1质粒载体简介
pLVX-ZsGreen1-C1质粒序列载体序列
LOCUS pLVX-ZsGreen1-C1 8763 bp DNA circular 7-OCT-2009
COMMENT Created by Clontech Laboratories Inc.
COMMENT Cat. No. 632566
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
LTR 1..635
/label=5'\LTR
misc_feature 685..822
/label=packaging\signal
misc_feature 4731..5322
/label=WPRE
CDS complement(7449..8445)
/label=Amp(R)
misc_binding 1303..1536
/label=RRE
misc_binding 636..653
/label=PBS
LTR 5525..6161
/label=3'\LTR
promoter 3589..4097
/label=PGK\Promoter
CDS 4118..4717
/label=Puro(R)
misc_feature 3574..3576
/label=Stop
misc_feature 3578..3580
/label=Stop
CDS 2807..3499
/label=ZsGreen1
misc_feature 2028..2151
/label=cPPT/CTS\(DNA\flap)
promoter 2185..2787
/label=CMV\IE\Promoter
rep_origin complement(6631..7304)
/label=pUC\ori
misc_feature 3582..3584
/label=Stop
misc_feature 3513..3565
/label=MCS
BASE COUNT 2162 a 2283 c 2311 g 2007 t
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
2761 tccatagaag acaccgactc tactagagga tctaccggtc gccaccatgg cccagtccaa
2821 gcacggcctg accaaggaga tgaccatgaa gtaccgcatg gagggctgcg tggacggcca
2881 caagttcgtg atcaccggcg agggcatcgg ctaccccttc aagggcaagc aggccatcaa
2941 cctgtgcgtg gtggagggcg gccccttgcc cttcgccgag gacatcttgt ccgccgcctt
3001 catgtacggc aaccgcgtgt tcaccgagta cccccaggac atcgtcgact acttcaagaa
3061 ctcctgcccc gccggctaca cctgggaccg ctccttcctg ttcgaggacg gcgccgtgtg
3121 catctgcaac gccgacatca ccgtgagcgt ggaggagaac tgcatgtacc acgagtccaa
3181 gttctacggc gtgaacttcc ccgccgacgg ccccgtgatg aagaagatga ccgacaactg
3241 ggagccctcc tgcgagaaga tcatccccgt gcccaagcag ggcatcttga agggcgacgt
3301 gagcatgtac ctgctgctga aggacggtgg ccgcttgcgc tgccagttcg acaccgtgta
3361 caaggccaag tccgtgcccc gcaagatgcc cgactggcac ttcatccagc acaagctgac
3421 ccgcgaggac cgcagcgacg ccaagaacca gaagtggcac ctgaccgagc acgccatcgc
3481 ctccggctcc gccttgccct ccggactcag atctcgagct caagcttcga attctgcagt
3541 cgacggtacc gcgggcccgg gatccaccgg atctagataa ctgatcataa ttctaccggg
3601 taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca
3661 cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc
3721 aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga
3781 agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc
3841 tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg
3901 gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg
3961 ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg
4021 gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct
4081 catctccggg cctttcgacc tgcagcccaa gcttaccatg accgagtaca agcccacggt
4141 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
4201 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
4261 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
4321 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
4381 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
4441 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
4501 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
4561 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
4621 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
4681 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaccg cgtctggaac aatcaacctc
4741 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
4801 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
4861 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
4921 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
4981 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
5041 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
5101 acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg
5161 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
5221 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
5281 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattaat tctgcagtcg
5341 agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg
5401 tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc
5461 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaagag
5521 gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta
5581 ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata
5641 tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga
5701 ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga
5761 cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc
5821 ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact
5881 ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct
5941 cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga
6001 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
6061 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
6121 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta
6181 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg ccttgacatt
6241 gctagcgttt taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
6301 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
6361 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
6421 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
6481 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
6541 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
6601 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
6661 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
6721 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
6781 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
6841 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
6901 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
6961 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
7021 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
7081 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
7141 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
7201 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
7261 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
7321 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
7381 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
7441 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
7501 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
7561 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
7621 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
7681 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
7741 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
7801 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
7861 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
7921 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
7981 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
8041 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
8101 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
8161 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
8221 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
8281 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
8341 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
8401 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat
8461 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
8521 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
8581 ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac
8641 cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc
8701 ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt
8761 agt
//
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