| 质粒名称: | EGFP-Rab11a-7 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 56444 |
| 存储实验室: | Michael Davidson |
|
载体骨架基本信息 |
|
| 载体名称: | EGFP |
| 载体出品公司: | Clontech |
| 空载体大小: | 4750bp |
| 完整质粒大小: | 5343bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 载体抗性: | Kanamycin |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Rab11a、NM_004663.3 |
| 物种来源: | H. sapiens (human) |
| 启动子: | CMV |
| 标签/融合蛋白: | EGFP (N terminal on backbone) |
| 克隆方法: | 未知 |
| 5’测序引物 | CMV-F |
| 3’测序引物 | SV40pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | EGFP-Rab11a-7 was a gift from Michael Davidson (Addgene plasmid # 56444 ; http://n2t.net/addgene:56444 ; RRID:Addgene_56444) |
| 参考文献部分: | The membrane-associated protein, supervillin, accelerates F-actin-dependent rapid integrin recycling and cell motility. Fang Z, Takizawa N, Wilson KA, Smith TC, Delprato A, Davidson MW, Lambright DG, Luna EJ. Traffic. 2010 Jun;11(6):782-99. doi: 10.1111/j.1600-0854.2010.01062.x. Epub 2010 Mar 17. 10.1111/j.1600-0854.2010.01062.x PubMed 20331534 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS EGFP-Rab11a-7 5343 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Localization: Ras GTPase, Excitation: 488, Emission: 507.
ACCESSION .
VERSION .
KEYWORDS EGFP-Rab11a-7
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5343)
AUTHORS Fang Z, Takizawa N, Wilson KA, Smith TC, Delprato A, Davidson MW,
Lambright DG, Luna EJ
TITLE The membrane-associated protein, supervillin, accelerates
F-actin-dependent rapid integrin recycling and cell motility.
JOURNAL Traffic. 2010 Jun;11(6):782-99. doi:
10.1111/j.1600-0854.2010.01062.x. Epub 2010 Mar 17.
PUBMED 20331534
REFERENCE 2 (bases 1 to 5343)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5343
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 1..304
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 305..508
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 459..479
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
regulatory 547..556
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 553..1269
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS 553..1269
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(598..619)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(859..878)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 1206..1227
/label=EGFP-C
/note="EGFP, forward primer"
polyA_signal 2071..2192
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2108..2127)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 2162..2181
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(2199..2654)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2336..2357)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 2548..2567
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 2681..2785
/gene="bla"
/label=AmpR promoter
promoter 2787..3144
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(2810..2830)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 2995..3130
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3057..3076
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 3179..3973
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(3233..3252)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 3843..3862
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(4161..4180)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 4205..4252
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 4581..5169
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5070..5089
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
541 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
601 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
661 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
721 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
781 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
841 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
901 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
961 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
1021 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
1081 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
1141 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
1201 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
1261 ctgtacaagt ccggactcag atctcgagcc atgggcaccc gcgacgacga gtacgactac
1321 ctctttaaag ttgtccttat tggagattct ggtgttggaa agagtaatct cctgtctcga
1381 tttactcgaa atgagtttaa tctggaaagc aagagcacca ttggagtaga gtttgcaaca
1441 agaagcatcc aggttgatgg aaaaacaata aaggcacaga tatgggacac agcagggcaa
1501 gagcgatatc gagctataac atcagcatat tatcgtggag ctgtaggtgc cttattggtt
1561 tatgacattg ctaaacatct cacatatgaa aatgtagagc gatggctgaa agaactgaga
1621 gatcatgctg atagtaacat tgttatcatg cttgtgggca ataagagtga tctacgtcat
1681 ctcagggcag ttcctacaga tgaagcaaga gcttttgcag aaaagaatgg tttgtcattc
1741 attgaaactt cggccctaga ctctacaaat gtagaagctg cttttcagac aattttaaca
1801 gagatttacc gcattgtttc tcagaagcaa atgtcagaca gacgcgaaaa tgacatgtct
1861 ccaagcaaca atgtggttcc tattcatgtt ccaccaacca ctgaaaacaa gccaaaggtg
1921 cagtgctgtc agaacatcta aggatccacc ggatctagat aactgatcat aatcagccat
1981 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
2041 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
2101 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
2161 tgtggtttgt ccaaactcat caatgtatct taacgcgtaa attgtaagcg ttaatatttt
2221 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
2281 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
2341 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
2401 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag
2461 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
2521 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
2581 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
2641 gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
2701 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
2761 tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt
2821 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
2881 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
2941 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
3001 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
3061 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
3121 tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat
3181 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
3241 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
3301 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
3361 agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
3421 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
3481 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
3541 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
3601 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
3661 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg
3721 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
3781 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
3841 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
3901 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
3961 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg
4021 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
4081 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
4141 caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct
4201 atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc
4261 ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa
4321 tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg
4381 ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt
4441 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
4501 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
4561 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
4621 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
4681 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
4741 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
4801 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
4861 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
4921 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
4981 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
5041 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
5101 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
5161 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
5221 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg
5281 cattagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
5341 ccg
//
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