| 出品公司: | Stratagene（Agilent） |
|---|---|
| 载体名称: | pRS414 |
| 质粒类型: | 酿酒酵母载体；酵母着丝粒质粒；穿梭载体 |
| 克隆方法: | -- |
| 高拷贝/低拷贝: | 多拷贝 |
| 启动子: | -- |
| 载体大小: | 4788 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | Ampicillin |
| 筛选标记: | TRP1 |
| 克隆宿主: | -- |
| 备注: | 着丝粒: CEN6；含a beta-gal报告基因；宿主菌株YPH499，YPH500，YPH501 |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pRS414载体质粒基本信息
pRS414质粒图谱载体图谱和pRS414载体序列质粒序列多克隆位点信息
pRS414质粒载体简介
pRS414质粒序列载体序列
LOCUS pRS414 4788 bp DNA SYN
DEFINITION pRS414
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..4788
/organism="pRS414"
/mol_type="other DNA"
misc_feature complement(29..51)
/label="pGEX_3_primer"
gene 468..1142
/label="TRP1"
/gene="TRP1"
CDS 468..1142
/label="ORF frame 3"
/translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNR
KRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGD
ESWQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSI
SDWVGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANF
VKNAKK*"
CDS complement(1479..2057)
/label="ORF frame 2"
/translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS
IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE
ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA
TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
misc_feature complement(1712..1855)
/label="lacZ_a"
/translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS
IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE
ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA
TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
misc_feature 1826..1848
/label="M13_pUC_fwd_primer"
/translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS
IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE
ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA
TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
promoter 1841..1857
/label="M13_forward20_primer"
/translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS
IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE
ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA
TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
promoter 1867..1885
/label="T7_promoter"
/translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS
IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE
ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA
TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
misc_feature 1910..1926
/label="pBluescriptKS_primer"
/translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS
IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE
ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA
TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
misc_feature complement(1961..1977)
/label="pBluescriptSK_primer"
/translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS
IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE
ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA
TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
promoter complement(2013..2032)
/label="T3_promoter"
/translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS
IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE
ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA
TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
promoter complement(2049..2067)
/label="M13_reverse_primer"
/translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS
IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE
ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA
TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
misc_feature complement(2066..2088)
/label="M13_pUC_rev_primer"
/translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS
IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE
ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA
TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
gene complement(3214..4074)
/label="Ampicillin"
/gene="Ampicillin"
/translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS
IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE
ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA
TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
CDS complement(3214..4074)
/label="ORF frame 1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
promoter complement(4116..4144)
/label="AmpR_promoter"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
misc_feature 4206..4724
/label="CEN6_ARS4"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accataaacg acattactat atatataata taggaagcat ttaatagaca gcatcgtaat
241 atatgtgtac tttgcagtta tgacgccaga tggcagtagt ggaagatatt ctttattgaa
301 aaatagcttg tcaccttacg tacaatcttg atccggagct tttctttttt tgccgattaa
361 gaattaattc ggtcgaaaaa agaaaaggag agggccaaga gggagggcat tggtgactat
421 tgagcacgtg agtatacgtg attaagcaca caaaggcagc ttggagtatg tctgttatta
481 atttcacagg tagttctggt ccattggtga aagtttgcgg cttgcagagc acagaggccg
541 cagaatgtgc tctagattcc gatgctgact tgctgggtat tatatgtgtg cccaatagaa
601 agagaacaat tgacccggtt attgcaagga aaatttcaag tcttgtaaaa gcatataaaa
661 atagttcagg cactccgaaa tacttggttg gcgtgtttcg taatcaacct aaggaggatg
721 ttttggctct ggtcaatgat tacggcattg atatcgtcca actgcatgga gatgagtcgt
781 ggcaagaata ccaagagttc ctcggtttgc cagttattaa aagactcgta tttccaaaag
841 actgcaacat actactcagt gcagcttcac agaaacctca ttcgtttatt cccttgtttg
901 attcagaagc aggtgggaca ggtgaacttt tggattggaa ctcgatttct gactgggttg
961 gaaggcaaga gagccccgaa agcttacatt ttatgttagc tggtggactg acgccagaaa
1021 atgttggtga tgcgcttaga ttaaatggcg ttattggtgt tgatgtaagc ggaggtgtgg
1081 agacaaatgg tgtaaaagac tctaacaaaa tagcaaattt cgtcaaaaat gctaagaaat
1141 aggttattac tgagtagtat ttatttaagt attgtttgtg cacttgccta tgcggtgtga
1201 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt
1261 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
1321 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
1381 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
1441 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
1501 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
1561 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
1621 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
1681 ccgctacagg gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
1741 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
1801 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag
1861 cgcgcgtaat acgactcact atagggcgaa ttgggtaccg ggccccccct cgaggtcgac
1921 ggtatcgata agcttgatat cgaattcctg cagcccgggg gatccactag ttctagagcg
1981 gccgccaccg cggtggagct ccagcttttg ttccctttag tgagggttaa ttgcgcgctt
2041 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
2101 caacatagga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgaggtaact
2161 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
2221 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
2281 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
2341 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
2401 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
2461 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
2521 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
2581 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
2641 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
2701 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
2761 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
2821 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
2881 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
2941 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
3001 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
3061 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
3121 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
3181 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
3241 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
3301 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
3361 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
3421 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
3481 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
3541 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
3601 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
3661 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
3721 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
3781 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
3841 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
3901 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
3961 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
4021 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
4081 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
4141 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
4201 acctgggtcc ttttcatcac gtgctataaa aataattata atttaaattt tttaatataa
4261 atatataaat taaaaataga aagtaaaaaa agaaattaaa gaaaaaatag tttttgtttt
4321 ccgaagatgt aaaagactct agggggatcg ccaacaaata ctacctttta tcttgctctt
4381 cctgctctca ggtattaatg ccgaattgtt tcatcttgtc tgtgtagaag accacacacg
4441 aaaatcctgt gattttacat tttacttatc gttaatcgaa tgtatatcta tttaatctgc
4501 ttttcttgtc taataaatat atatgtaaag tacgcttttt gttgaaattt tttaaacctt
4561 tgtttatttt tttttcttca ttccgtaact cttctacctt ctttatttac tttctaaaat
4621 ccaaatacaa aacataaaaa taaataaaca cagagtaaat tcccaaatta ttccatcatt
4681 aaaagatacg aggcgcgtgt aagttacagg caagcgatcc gtcctaagaa accattatta
4741 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtc
//
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