| 质粒名称: | BTV |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 84771 |
| 存储实验室: | David Erle |
|
载体骨架基本信息 |
|
| 载体名称: | BdLV |
| 载体出品公司: | Luigi Naldini , PMID 15619618 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | BdLV中的组成性双向启动子被一个双向四环素反应启动子取代。在EGFP开放阅读框和polyA信号之间插入一个具有MluI、SbfI和PacI位点的连接子，用于3’UTR序列的亚克隆 |
| 载体类型: | 哺乳细胞表达,慢病毒 |
| 筛选标记: | -- |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | -- |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | EGFP-C |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | BTV was a gift from David Erle (Addgene plasmid # 84771 ; http://n2t.net/addgene:84771 ; RRID:Addgene_84771) |
| 参考文献部分: | Massively parallel functional annotation of 3' untranslated regions. Zhao W, Pollack JL, Blagev DP, Zaitlen N, McManus MT, Erle DJ. Nat Biotechnol. 2014 Apr;32(4):387-91. doi: 10.1038/nbt.2851. Epub 2014 Mar 16. 10.1038/nbt.2851 PubMed 24633241 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS BTV 9363 bp ds-DNA circular SYN 13-MAY-2021
DEFINITION bidirectional tetracycline-regulated viral reporter that measures
the effects of 3' UTR sequences on mRNA and protein production.
ACCESSION .
VERSION .
KEYWORDS BTV
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9363)
AUTHORS Zhao W, Pollack JL, Blagev DP, Zaitlen N, McManus MT, Erle DJ
TITLE Massively parallel functional annotation of 3' untranslated regions.
JOURNAL Nat Biotechnol. 2014 Apr;32(4):387-91. doi: 10.1038/nbt.2851. Epub
2014 Mar 16.
PUBMED 24633241
REFERENCE 2 (bases 1 to 9363)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9363
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(11..28)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(182..770)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(262..281)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(941..1801)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 1564..1583
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(1802..1906)
/gene="bla"
/label=AmpR promoter
rep_origin complement(1932..2387)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2069..2090)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 2281..2300
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 2514..2536
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 2528..2545
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 2529..2545
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 2555..2574
/label=T7
/note="T7 promoter, forward primer"
promoter 2555..2573
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
rep_origin 2594..2729
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind complement(2648..2667)
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
polyA_signal 2769..2890
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2780..2799)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
LTR 2962..3195
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
misc_feature 3282..3870
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
CDS 3388..3399
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind 3797..3817
/label=WPRE-R
/note="WPRE, reverse primer"
promoter complement(4980..5018)
/label=minimal CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
protein_bind 5051..5069
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 5093..5111
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 5135..5153
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 5177..5195
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 5219..5237
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 5261..5279
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
regulatory 5435..5444
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 5441..6157
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(5486..5507)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(5747..5766)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 6094..6115
/label=EGFP-C
/note="EGFP, forward primer"
polyA_signal 6443..6577
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
misc_feature 6714..6831
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
CDS complement(7098..7142)
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 7327..7560
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 8053..8178
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
LTR 8225..8405
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
promoter complement(8420..8623)
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind complement(8449..8469)
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
enhancer 8624..9003
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
primer_bind complement(9104..9124)
/label=T3
/note="T3 promoter, forward primer"
promoter complement(9104..9122)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(9143..9159)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(9143..9159)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(9156..9178)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 9167..9183
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(9191..9221)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 9236..9257
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
ORIGIN
1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
1921 aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
1981 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
2041 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
2101 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
2161 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
2221 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
2281 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
2341 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt
2401 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
2461 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
2521 acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac gactcactat agggcgaatt
2581 gggtacgtcc ctaggcctcc aaaaaagcct cctcactact tctggaatag ctcagaggcc
2641 gaggcggcct cggcctctgc ataaataaaa aaaattagtc agccatgggg cggagaatgg
2701 gcggaactgg gcggagttag gggcgggatg ggcggagtta ggggcgggat agctagagcc
2761 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
2821 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
2881 taaacaagtt cctctcactc tctgatattc atttctttgc aagttataaa tactgaataa
2941 taagatgaca tgaactacta ctgctagaga ttttccacac tgactaaaag ggtctgaggg
3001 atctctagtt accagagtca cacaacagac gggcacacac tacttgaagc actcaaggca
3061 agctttattg aggcttaagc agtgggttcc ctagttagcc agagagctcc caggctcaga
3121 tctggtctaa ccagagagac ccagtacaag caaaaagcag atcttgtctt cgttgggagt
3181 gaattagccc ttccagtccc cccttttctt ttaaaaagtg gctaagatct acagctgcct
3241 tgtaagtcat tggtcttaaa ggtaccgagc tcgaattcca ggcggggagg cggcccaaag
3301 ggagatccga ctcgtctgag ggcgaaggcg aagacgcgga agaggccgca gagccggcag
3361 caggccgcgg gaaggaaggt ccgctggatt gagggccgaa gggacgtagc agaaggacgt
3421 cccgcgcaga atccaggtgg caacacaggc gagcagccat ggaaaggacg tcagcttccc
3481 cgacaacacc acggaattgt cagtgcccaa cagccgagcc cctgtccagc agcgggcaag
3541 gcaggcggcg atgagttccg ccgtggcaat agggaggggg aaagcgaaag tcccggaaag
3601 gagctgacag gtggtggcaa tgccccaacc agtgggggtt gcgtcagcaa acacagtgca
3661 caccacgcca cgttgcctga caacgggcca caactcctca taaagagaca gcaaccagga
3721 tttatacaag gaggagaaaa tgaaagccat acgggaagca atagcatgat acaaaggcat
3781 taaagcagcg tatccacata gcgtaaaagg agcaacatag ttaagaatac cagtcaatct
3841 ttcacaaatt ttgtaatcca gaggttgatt gtcgactcta gactcgactc tagaggatcc
3901 ccctgttcca cctcttgaag gctatgtagg ccacaaggcc cacaaccaca gcagccagga
3961 tggagcaata gacagggatg aggttgtcgg tggtgcctcg ggtcaccacg ggctgggagc
4021 tgcccatcac tgtggtcacc acacctgcca ccgtgctggc tatgaggtct tgttctggag
4081 gtgcctcagg ctcctgggtg ctgggggctg tgctgtccga gccctctggg ggtgtggacc
4141 gtgtaatcca acggccaggg atctcctcgc actcggcgtc ggcccagcgt gtgcactcgc
4201 ggagctggcg ctcggtgtcc tcgcacacgg tgcagggcag gcacgggtcc acgtggttgg
4261 cctcgtcgga atacgtgccg tcggggcact cctcgcacac ggtgttctgc ttgtcctggc
4321 aggagaacac gaggcccgag cccgcctcgc acacgcggca cgcctcgcag cgcccagtcg
4381 tctcatcctg gtagtagccg taggcgcagc ggcacacggc gtcgtcggcc tccacgcacg
4441 gcgccgacat gctctggagc cccacgcact cggtgcacgg cttgcacggc tcggtcgcgc
4501 tcaccacgtc ggagaacgtc acgctgtcca ggcagggctc acacacggtc tggttggctc
4561 cacaaggctg ggccacaccc tcgcccaggt tgcaggcttt gcagcactca ccgctgtgtg
4621 tgtacaggcc tgtggggcat gcctccttgg cacctccaag ggacaccccc agaagcagca
4681 acagcagcag gcgcggcccg tccatggcgc ggccggtggc acctgccccc atcgcccgcc
4741 tcccgcggca gcgctcgact tccagctcgg tccgctttgc ggactgatgg ggctgcgctg
4801 cgctgcgctc cagcgccccc cctgcccgcc ggagctggcc gcggcccgaa ttccctagag
4861 gatccccggg taccgagctc gaattcgggg ccgcggaggc tggatcggtc ccggtgtctt
4921 ctatggaggt caaaacagcg tggatggcgt ctccaggcga tctgacggtt cactaaacga
4981 gctctgctta tataggcctc ccaccgtaca cgcctacctc gacccgggta ccgagctcga
5041 ctttcacttt tctctatcac tgatagggag tggtaaactc gactttcact tttctctatc
5101 actgataggg agtggtaaac tcgactttca cttttctcta tcactgatag ggagtggtaa
5161 actcgacttt cacttttctc tatcactgat agggagtggt aaactcgact ttcacttttc
5221 tctatcactg atagggagtg gtaaactcga ctttcacttt tctctatcac tgatagggag
5281 tggtaaactc gacctatata agcagagctc gtttagtgaa ccgtcagatc gcctggagac
5341 gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc ctccgcggcc
5401 ccgaattcct gcagcggccc tagcgctacc ggtcgccacc atggtgagca agggcgagga
5461 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
5521 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
5581 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
5641 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
5701 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
5761 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
5821 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
5881 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
5941 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
6001 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
6061 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
6121 cgccgggatc actctcggca tggacgagct gtacaagtag cggccggccg cgtcgaccta
6181 gttctagcta gcaactacgc gtagtcgata tccttacctg cagggccttt aattaaactg
6241 gctagcttag tcatgcaccg gtggatccag accacctccc ctgcgagcta agctggacag
6301 ccaatgacgg gtaagagagt gacatttttc actaacctaa gacaggaggg ccgtcagagc
6361 tactgcctaa tccaaagacg ggtaaaagtg ataaaaatgt atcactccaa cctaagacag
6421 gcgcagcttc cgagggattt gagatccaga catgataaga tacattgatg agtttggaca
6481 aaccaaaact agaatgcagt gaaaaaaatg ccttatttgt gaaatttgtg atgctattgc
6541 cttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
6601 tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa
6661 atgtggtatg gctgattatg atcagatctc tcgaggctag tctcgtgatc gataaaattt
6721 tgaatttttg taatttgttt ttgtaattct ttagtttgta tgtctgttgc tattatgtct
6781 actattcttt cccctgcact gtacccccca atcccccctt ttcttttaaa agttaaccga
6841 taccgtcgag atccgttcac taatcgaatg gatctgtctc tgtctctctc tccaccttct
6901 tcttctattc cttcgggcct gtcgggtccc ctcggggttg ggaggtgggt ctgaaacgat
6961 aatggtgaat atccctgcct aactctattc actatagaaa gtacagcaaa aactattctt
7021 aaacctacca agcctcctac tatcattatg aataatttta tataccacag ccaatttgtt
7081 atgttaaacc aattccacaa acttgcccat ttatctaatt ccaataattc ttgttcattc
7141 ttttcttgct ggttttgcga ttcttcaatt aaggagtgta ttaagcttgt gtaattgtta
7201 atttctctgt cccactccat ccaggtcgtg tgattccaaa tctgttccag agatttatta
7261 ctccaactag cattccaagg cacagcagtg gtgcaaatga gttttccaga gcaaccccaa
7321 atccccagga gctgttgatc ctttaggtat ctttccacag ccaggattct tgcctggagc
7381 tgcttgatgc cccagactgt gagttgcaac agatgctgtt gcgcctcaat agccctcagc
7441 aaattgttct gctgctgcac tataccagac aataattgtc tggcctgtac cgtcagcgtc
7501 attgacgctg cgcccatagt gcttcctgct gctcccaaga acccaaggaa caaagctcct
7561 attcccactg ctcttttttc tctctgcacc actcttctct ttgccttggt gggtgctact
7621 cctaatggtt caatttttac tactttatat ttatataatt cacttctcca attgtccctc
7681 atatctcctc ctccaggtct gaagatcagc ggccgcttgc tgtgcggtgg tcttactttt
7741 gttttgctct tcctctatct tgtctaaagc ttccttggtg tcttttatct ctatcctttg
7801 atgcacacaa tagagggttg ctactgtatt atataatgat ctaagttctt ctgatcctgt
7861 ctgaagggat ggttgtagct gtcccagtat ttgtctacag ccttctgatg tttctaacag
7921 gccaggatta actgcgaatc gttctagctc cctgcttgcc catactatat gttttaattt
7981 atattttttc tttccccctg gccttaaccg aattttttcc catcgcgatc taattctccc
8041 ccgcttaata ctgacgctct cgcacccatc tctctccttc tagcctccgc tagtcaaaat
8101 ttttggcgta ctcaccagtc gccgcccctc gcctcttgcc gtgcgcgctt cagcaagccg
8161 agtcctgcgt cgagagagct cctctggttt ccctttcgct ttcaagtccc tgttcgggcg
8221 ccactgctag agattttcca cactgactaa aagggtctga gggatctcta gttaccagag
8281 tcacacaaca gacgggcaca cactacttga agcactcaag gcaagcttta ttgaggctta
8341 agcagtgggt tccctagtta gccagagagc tcccaggctc agatctggtc taaccagaga
8401 gaccccggtt cactaaacga gctctgctta tatagacctc ccaccgtaca cgcctaccgc
8461 ccatttgcgt caatggggcg gagttgttac gacattttgg aaagtcccgt tgattttggt
8521 gccaaaacaa actcccattg acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa
8581 accgctatcc acgcccattg atgtactgcc aaaaccgcat caccatggta atagcgatga
8641 ctaatacgta gatgtactgc caagtaggaa agtcccataa ggtcatgtac tgggcataat
8701 gccaggcggg ccatttaccg tcattgacgt caataggggg cgtacttggc atatgataca
8761 cttgatgtac tgccaagtgg gcagtttacc gtaaatactc cacccattga cgtcaatgga
8821 aagtccctat tggcgttact atgggaacat acgtcattat tgacgtcaat gggcgggggt
8881 cgttgggcgg tcagccaggc gggccattta ccgtaagtta tgtaacgcgg aactccatat
8941 atgggctatg aactaatgac cccgtaattg attactatta ataactagtc aataatcaat
9001 gtcaacatgg cggtaatgtt ggacatgagc caatataaat gtacatatta tgatatggat
9061 acaacgtatg caatggccaa gcttgcagct ccagcttttg ttccctttag tgagggttaa
9121 ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
9181 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
9241 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
9301 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
9361 gct
//
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