质粒名称: | ABE8.20-m |
---|---|
出品公司: | Addgene |
目录编号: | 136300 |
存储实验室: | Nicole Gaudelli |
载体骨架基本信息 |
|
载体名称: | pBR322, beta-lactamase maintenance, CMV promoter |
载体出品公司: | -- |
空载体大小: | 3400 bp |
完整质粒大小: | 8166 bp |
载体修饰: | -- |
载体类型: | 哺乳细胞表达 |
筛选标记: | -- |
载体抗性: | 氨苄霉素 |
生长温度: | 37 ℃ |
宿主菌: | NEB Stable |
拷贝数: | 高拷贝 |
插入基因信息 |
|
插入基因名称: | ABE8.20-m |
物种来源: | -- |
插入大小: | 4800 bp |
基因突变: | D10A in S. pyogenes Cas9, TadA mutations described in manuscript |
GenBank编号 | -- |
启动子: | -- |
标签/融合蛋白: | C-terminal BPNLS(插入件上的C端子) |
克隆方法: | Ligation Independent克隆 |
5’克隆位点: | -- |
3’克隆位点: | -- |
5’测序引物 | T7 |
3’测序引物 | M13R |
文献引用方法 |
|
材料和方法部分: | ABE8.20-m was a gift from Nicole Gaudelli (Addgene plasmid # 136300 ; http://n2t.net/addgene:136300 ; RRID:Addgene_136300) |
参考文献部分: | Directed evolution of adenine base editors with increased activity and therapeutic application. Gaudelli NM, Lam DK, Rees HA, Sola-Esteves NM, Barrera LA, Born DA, Edwards A, Gehrke JM, Lee SJ, Liquori AJ, Murray R, Packer MS, Rinaldi C, Slaymaker IM, Yen J, Young LE, Ciaramella G. Nat Biotechnol. 2020 Apr 13. pii: 10.1038/s41587-020-0491-6. doi: 10.1038/s41587-020-0491-6. 10.1038/s41587-020-0491-6 PubMed 32284586 |
买家导航
ABE8.20m载体基本信息
ABE8.20m载体图谱质粒图谱和多克隆位点信息
ABE8.20m载体简介
ABE8.20m载体序列
LOCUS ABE8.20-m 8166 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION expresses ABE8.20-m in mammalian cells. ACCESSION . VERSION . KEYWORDS ABE8.20-m SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8166) AUTHORS Gaudelli NM, Lam DK, Rees HA, Sola-Esteves NM, Barrera LA, Born DA, Edwards A, Gehrke JM, Lee SJ, Liquori AJ, Murray R, Packer MS, Rinaldi C, Slaymaker IM, Yen J, Young LE, Ciaramella G TITLE Directed evolution of adenine base editors with increased activity and therapeutic application. JOURNAL Nat Biotechnol. 2020 Apr 13. pii: 10.1038/s41587-020-0491-6. doi: 10.1038/s41587-020-0491-6. PUBMED 32284586 REFERENCE 2 (bases 1 to 8166) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8166 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 103..482 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 483..686 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 637..657 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 728..747 /label=T7 /note="T7 promoter, forward primer" promoter 728..746 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 763..1260 /codon_start=1 /product="DNA adenosine deaminase evolved from the E. coli tRNA adenosine deaminase TadA (Gaudelli et al., 2017)" /label=TadA*(7.10) /translation="SEVEFSHEYWMRHALTLAKRARDEREVPVGAVLVLNNRVIGEGWN RAIGLHDPTAHAEIMALRQGGLVMQNYRLYDATLYSTFEPCVMCAGAMIHSRIGRVVFG VRNAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLCRFFRMPRRVFNAQKKAQS STD" CDS 1357..5457 /codon_start=1 /product="nickase mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=Cas9(D10A) /note="generates RNA-guided single strand nicks in DNA due to the D10A mutation in the RuvC catalytic domain" /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD ATLIHQSITGLYETRIDLSQLGGD" CDS 5503..5523 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 5532..5549 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" primer_bind complement(5572..5589) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 5578..5802 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" primer_bind complement(5873..5889) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(5873..5889) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(5886..5908) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 5897..5913 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(5921..5951) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 5966..5987 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(6104..6121) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(6275..6863) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(6355..6374) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(7034..7894) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 7657..7676 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(7895..7999) /gene="bla" /label=AmpR promoter primer_bind complement(8078..8097) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" ORIGIN 1 aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc 61 gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta 121 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg 181 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga 241 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat 301 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa 361 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca 421 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca 481 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat 541 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg 601 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac 661 ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc tagagatccg 721 cggccgctaa tacgactcac tatagggaga gccgccacca tgtccgaagt cgagttttcc 781 catgagtact ggatgagaca cgcattgact ctcgcaaaga gggctcgaga tgaacgcgag 841 gtgcccgtgg gggcagtact cgtgctcaac aatcgcgtaa tcggcgaagg ttggaatagg 901 gcaatcggac tccacgaccc cactgcacat gcggaaatca tggcccttcg acagggaggg 961 cttgtgatgc agaattatcg actttatgat gcgacgctgt actcgacgtt tgaaccttgc 1021 gtaatgtgcg cgggagctat gattcactcc cgcattggac gagttgtatt cggtgttcgc 1081 aacgccaaga cgggtgccgc aggttcactg atggacgtgc tgcatcatcc aggcatgaac 1141 caccgggtag aaatcacaga aggcatattg gcggacgaat gtgcggcgct gttgtgtcgt 1201 ttttttcgca tgcccaggcg ggtctttaac gcccagaaaa aagcacaatc ctctactgac 1261 tctggtggtt cttctggtgg ttctagcggc agcgagactc ccgggacctc agagtccgcc 1321 acacccgaaa gttctggtgg ttcttctggt ggttctgaca agaagtacag catcggcctg 1381 gccatcggca ccaactctgt gggctgggcc gtgatcaccg acgagtacaa ggtgcccagc 1441 aagaaattca aggtgctggg caacaccgac cggcacagca tcaagaagaa cctgatcgga 1501 gccctgctgt tcgacagcgg cgaaacagcc gaggccaccc ggctgaagag aaccgccaga 1561 agaagataca ccagacggaa gaaccggatc tgctatctgc aagagatctt cagcaacgag 1621 atggccaagg tggacgacag cttcttccac agactggaag agtccttcct ggtggaagag 1681 gataagaagc acgagcggca ccccatcttc ggcaacatcg tggacgaggt ggcctaccac 1741 gagaagtacc ccaccatcta ccacctgaga aagaaactgg tggacagcac cgacaaggcc 1801 gacctgcggc tgatctatct ggccctggcc cacatgatca agttccgggg ccacttcctg 1861 atcgagggcg acctgaaccc cgacaacagc gacgtggaca agctgttcat ccagctggtg 1921 cagacctaca accagctgtt cgaggaaaac cccatcaacg ccagcggcgt ggacgccaag 1981 gccatcctgt ctgccagact gagcaagagc agacggctgg aaaatctgat cgcccagctg 2041 cccggcgaga agaagaatgg cctgttcgga aacctgattg ccctgagcct gggcctgacc 2101 cccaacttca agagcaactt cgacctggcc gaggatgcca aactgcagct gagcaaggac 2161 acctacgacg acgacctgga caacctgctg gcccagatcg gcgaccagta cgccgacctg 2221 tttctggccg ccaagaacct gtccgacgcc atcctgctga gcgacatcct gagagtgaac 2281 accgagatca ccaaggcccc cctgagcgcc tctatgatca agagatacga cgagcaccac 2341 caggacctga ccctgctgaa agctctcgtg cggcagcagc tgcctgagaa gtacaaagag 2401 attttcttcg accagagcaa gaacggctac gccggctaca ttgacggcgg agccagccag 2461 gaagagttct acaagttcat caagcccatc ctggaaaaga tggacggcac cgaggaactg 2521 ctcgtgaagc tgaacagaga ggacctgctg cggaagcagc ggaccttcga caacggcagc 2581 atcccccacc agatccacct gggagagctg cacgccattc tgcggcggca ggaagatttt 2641 tacccattcc tgaaggacaa ccgggaaaag atcgagaaga tcctgacctt ccgcatcccc 2701 tactacgtgg gccctctggc caggggaaac agcagattcg cctggatgac cagaaagagc 2761 gaggaaacca tcaccccctg gaacttcgag gaagtggtgg acaagggcgc ttccgcccag 2821 agcttcatcg agcggatgac caacttcgat aagaacctgc ccaacgagaa ggtgctgccc 2881 aagcacagcc tgctgtacga gtacttcacc gtgtataacg agctgaccaa agtgaaatac 2941 gtgaccgagg gaatgagaaa gcccgccttc ctgagcggcg agcagaaaaa ggccatcgtg 3001 gacctgctgt tcaagaccaa ccggaaagtg accgtgaagc agctgaaaga ggactacttc 3061 aagaaaatcg agtgcttcga ctccgtggaa atctccggcg tggaagatcg gttcaacgcc 3121 tccctgggca cataccacga tctgctgaaa attatcaagg acaaggactt cctggacaat 3181 gaggaaaacg aggacattct ggaagatatc gtgctgaccc tgacactgtt tgaggacaga 3241 gagatgatcg aggaacggct gaaaacctat gcccacctgt tcgacgacaa agtgatgaag 3301 cagctgaagc ggcggagata caccggctgg ggcaggctga gccggaagct gatcaacggc 3361 atccgggaca agcagtccgg caagacaatc ctggatttcc tgaagtccga cggcttcgcc 3421 aacagaaact tcatgcagct gatccacgac gacagcctga cctttaaaga ggacatccag 3481 aaagcccagg tgtccggcca gggcgatagc ctgcacgagc acattgccaa tctggccggc 3541 agccccgcca ttaagaaggg catcctgcag acagtgaagg tggtggacga gctcgtgaaa 3601 gtgatgggcc ggcacaagcc cgagaacatc gtgatcgaaa tggccagaga gaaccagacc 3661 acccagaagg gacagaagaa cagccgcgag agaatgaagc ggatcgaaga gggcatcaaa 3721 gagctgggca gccagatcct gaaagaacac cccgtggaaa acacccagct gcagaacgag 3781 aagctgtacc tgtactacct gcagaatggg cgggatatgt acgtggacca ggaactggac 3841 atcaaccggc tgtccgacta cgatgtggac catatcgtgc ctcagagctt tctgaaggac 3901 gactccatcg acaacaaggt gctgaccaga agcgacaaga accggggcaa gagcgacaac 3961 gtgccctccg aagaggtcgt gaagaagatg aagaactact ggcggcagct gctgaacgcc 4021 aagctgatta cccagagaaa gttcgacaat ctgaccaagg ccgagagagg cggcctgagc 4081 gaactggata aggccggctt catcaagaga cagctggtgg aaacccggca gatcacaaag 4141 cacgtggcac agatcctgga ctcccggatg aacactaagt acgacgagaa tgacaagctg 4201 atccgggaag tgaaagtgat caccctgaag tccaagctgg tgtccgattt ccggaaggat 4261 ttccagtttt acaaagtgcg cgagatcaac aactaccacc acgcccacga cgcctacctg 4321 aacgccgtcg tgggaaccgc cctgatcaaa aagtacccta agctggaaag cgagttcgtg 4381 tacggcgact acaaggtgta cgacgtgcgg aagatgatcg ccaagagcga gcaggaaatc 4441 ggcaaggcta ccgccaagta cttcttctac agcaacatca tgaacttttt caagaccgag 4501 attaccctgg ccaacggcga gatccggaag cggcctctga tcgagacaaa cggcgaaacc 4561 ggggagatcg tgtgggataa gggccgggat tttgccaccg tgcggaaagt gctgagcatg 4621 ccccaagtga atatcgtgaa aaagaccgag gtgcagacag gcggcttcag caaagagtct 4681 atcctgccca agaggaacag cgataagctg atcgccagaa agaaggactg ggaccctaag 4741 aagtacggcg gcttcgacag ccccaccgtg gcctattctg tgctggtggt ggccaaagtg 4801 gaaaagggca agtccaagaa actgaagagt gtgaaagagc tgctggggat caccatcatg 4861 gaaagaagca gcttcgagaa gaatcccatc gactttctgg aagccaaggg ctacaaagaa 4921 gtgaaaaagg acctgatcat caagctgcct aagtactccc tgttcgagct ggaaaacggc 4981 cggaagagaa tgctggcctc tgccggcgaa ctgcagaagg gaaacgaact ggccctgccc 5041 tccaaatatg tgaacttcct gtacctggcc agccactatg agaagctgaa gggctccccc 5101 gaggataatg agcagaaaca gctgtttgtg gaacagcaca agcactacct ggacgagatc 5161 atcgagcaga tcagcgagtt ctccaagaga gtgatcctgg ccgacgctaa tctggacaaa 5221 gtgctgtccg cctacaacaa gcaccgggat aagcccatca gagagcaggc cgagaatatc 5281 atccacctgt ttaccctgac caatctggga gcccctgccg ccttcaagta ctttgacacc 5341 accatcgacc ggaagaggta caccagcacc aaagaggtgc tggacgccac cctgatccac 5401 cagagcatca ccggcctgta cgagacacgg atcgacctgt ctcagctggg aggtgacgag 5461 ggagctgata agcgcaccgc cgatggttcc gagttcgaaa gccccaagaa gaagaggaaa 5521 gtctaaccgg tcatcatcac catcaccatt gagtttaaac ccgctgatca gcctcgactg 5581 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg 5641 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga 5701 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg 5761 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa 5821 ccagctgggg ctcgataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc 5881 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca 5941 taaagtgtaa agcctagggt gcctaatgag tgagctaact cacattaatt gcgttgcgct 6001 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac 6061 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc 6121 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 6181 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 6241 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 6301 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 6361 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 6421 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 6481 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 6541 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 6601 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 6661 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag 6721 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 6781 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 6841 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 6901 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca 6961 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa 7021 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat 7081 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct 7141 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt 7201 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat 7261 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta 7321 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg 7381 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt 7441 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg 7501 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg 7561 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc 7621 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa 7681 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac 7741 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt 7801 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg 7861 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa 7921 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata 7981 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg 8041 gagatcgatc tcccgatccc ctagggtcga ctctcagtac aatctgctct gatgccgcat 8101 agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca 8161 aaattt //