| 质粒名称: | pLIX_403 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 41395 |
| 存储实验室: | David Root |
|
载体骨架基本信息 |
|
| 载体名称: | pCW57.1 |
| 载体出品公司: | Addgene plasmid 41393 |
| 载体抗性: | 氯霉素和氨苄青霉素 |
| 空载体大小: | 9396 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，慢病毒；Gateway Destination载体，强力霉素诱导 |
| 筛选标记: | 嘌呤霉素 |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | TRE promoter, Tet ON |
| 标签/融合蛋白: | V5（主干上的C终端） |
| 克隆方法: | Gateway克隆 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | LNCX |
| 3' 测序引物 | O.PGK1b-R (GAACGGACGTGAAGAATGTG) |
|
文献引用方法 |
|
| 材料和方法部分: | pLIX_403 was a gift from David Root (Addgene plasmid # 41395 ; http://n2t.net/addgene:41395 ; RRID:Addgene_41395)<img alt="pLIX_403质粒图谱" src="/uploads/载体图谱/pLIX_403质粒图谱.png" /><br /> |
买家导航
pLIX403载体基本信息
pLIX403载体图谱质粒图谱和多克隆位点信息
pLIX403载体简介
pLIX403载体序列
LOCUS pLIX_403 9396 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Inducible lentiviral expression, TRE-gateway-V5; PGK-puro-2A-rtTA.
ACCESSION .
VERSION .
KEYWORDS pLIX_403
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9396)
TITLE Root Lab pLEX and pLIX Gateway backbones
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 9396)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9396
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 41..158
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 212..526
/label=tight TRE promoter
/note="Tet-responsive promoter PTight, consisting of seven
tet operator sequences followed by the minimal CMV
promoter"
protein_bind 220..238
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 256..274
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 291..309
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 327..345
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 363..381
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 398..416
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 434..452
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
primer_bind 499..523
/label=LNCX
/note="Human CMV promoter, forward primer"
protein_bind 547..671
/gene="mutant version of attR"
/label=attR1
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
promoter 696..726
/label=lac UV5 promoter
/note="E. coli lac promoter with an ""up"" mutation"
CDS 780..1439
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
primer_bind complement(846..865)
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
CDS 1781..2086
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/label=ccdB
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
primer_bind 1965..1984
/label=ccdB-fwd
/note="ccdB gene, forward primer"
protein_bind complement(2127..2251)
/gene="mutant version of attR"
/label=attR2
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
CDS 2256..2297
/codon_start=1
/product="epitope tag from simian virus 5"
/label=V5 tag
/translation="GKPIPNPLLGLDST"
CDS 2311..2328
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
promoter 2338..2848
/label=hPGK promoter
/note="human phosphoglycerate kinase 1 promoter"
primer_bind 2767..2785
/label=hPGK-F
/note="Human PGK promoter, forward primer"
CDS 2858..3454
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(2858..2877)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 3354..3374
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
CDS 3455..3508
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label=T2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
CDS 3509..4255
/codon_start=1
/product="improved tetracycline-controlled transactivator"
/label=rtTA-Advanced
/note="In the Tet-On(R) system, rtTA-Advanced binds to the
Tet-responsive element and stimulates transcription only in
the presence of tetracycline or doxycycline."
/translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
DALDDFDLDMLPADALDDFDLDMLPG"
primer_bind complement(4295..4315)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(4713..4724)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 4929..5162
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 5234..5355
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5271..5290)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 5325..5344
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 5395..5530
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 5457..5476
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
promoter 5530..5601
/gene="bla"
/label=AmpR promoter
CDS 5602..6462
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(5820..5839)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 6633..7221
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 7122..7141
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 7375..7392
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 7509..7530
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 7545..7575
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7583..7599
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 7588..7610
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 7607..7623
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 7607..7623
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 7642..7662
/label=T3
/note="T3 promoter, forward primer"
promoter 7644..7662
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
promoter 7690..7916
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 7917..8097
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 8144..8269
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 8762..8995
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
ORIGIN
1 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
61 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
181 gagatccact ttggccgcga atcgatatgt cgagtttact ccctatcagt gatagagaac
241 gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag
301 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
361 actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt
421 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg
481 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gaattggcta
541 gcatcaacaa gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat
601 atattaaatt agattttgca taaaaaacag actacataat actgtaaaac acaacatatc
661 cagtcactat ggcggccgca ttaggcaccc caggctttac actttatgct tccggctcgt
721 ataatgtgtg gattttgagt taggatccgt cgagattttc aggagctaag gaagctaaaa
781 tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac
841 attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata
901 ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc
961 acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg
1021 agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa
1081 cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt
1141 cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga
1201 atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg
1261 ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg
1321 acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg
1381 tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaaa
1441 gatctggatc cggcttacta aaagccagat aacagtatgc gtatttgcgc gctgattttt
1501 gcggtataag aatatatact gatatgtata cccgaagtat gtcaaaaaga ggtatgctat
1561 gaagcagcgt attacagtga cagttgacag cgacagctat cagttgctca aggcatatat
1621 gatgtcaata tctccggtct ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt
1681 gccgaacgct ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg gtttattgaa
1741 atgaacggct cttttgctga cgagaacagg ggctggtgaa atgcagttta aggtttacac
1801 ctataaaaga gagagccgtt atcgtctgtt tgtggatgta cagagtgata ttattgacac
1861 gcccgggcga cggatggtga tccccctggc cagtgcacgt ctgctgtcag ataaagtctc
1921 ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga
1981 tatggccagt gtgccggtct ccgttatcgg ggaagaagtg gctgatctca gccaccgcga
2041 aaatgacatc aaaaacgcca ttaacctgat gttctgggga atataaatgt caggctccct
2101 tatacacagc cagtctgcag gtcgaccata gtgactggat atgttgtgtt ttacagtatt
2161 atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg
2221 tttctcgttc agctttcttg tacaaagtgg ttgcaggaaa gccaatccca aacccactcc
2281 taggtctcga cagtacagca tagaccggtc caccaccacc accaccacta aggatccggg
2341 gttggggttg cgccttttcc aaggcagccc tgggtttgcg cagggacgcg gctgctctgg
2401 gcgtggttcc gggaaacgca gcggcgccga ccctgggtct cgcacattct tcacgtccgt
2461 tcgcagcgtc acccggatct tcgccgctac ccttgtgggc cccccggcga cgcttcctgc
2521 tccgccccta agtcgggaag gttccttgcg gttcgcggcg tgccggacgt gacaaacgga
2581 agccgcacgt ctcactagta ccctcgcaga cggacagcgc cagggagcaa tggcagcgcg
2641 ccgaccgcga tgggctgtgg ccaatagcgg ctgctcagca gggcgcgccg agagcagcgg
2701 ccgggaaggg gcggtgcggg aggcggggtg tggggcggta gtgtgggccc tgttcctgcc
2761 cgcgcggtgt tccgcattct gcaagcctcc ggagcgcacg tcggcagtcg gctccctcgt
2821 tgaccgaatc accgacctct ctccccagca attcaccatg accgagtaca agcccacggt
2881 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
2941 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
3001 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
3061 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
3121 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
3181 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
3241 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
3301 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
3361 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
3421 gcgcacctgg tgcatgaccc gcaagcccgg tgccgaaggt agaggttctc tcctcacttg
3481 tggtgatgtt gaagaaaacc ctggtccaat gtctagactg gacaagagca aagtcataaa
3541 cggagctctg gaattactca atggtgtcgg tatcgaaggc ctgacgacaa ggaaactcgc
3601 tcaaaagctg ggagttgagc agcctaccct gtactggcac gtgaagaaca agcgggccct
3661 gctcgatgcc ctgccaatcg agatgctgga caggcatcat acccacttct gccccctgga
3721 aggcgagtca tggcaagact ttctgcggaa caacgccaag tcataccgct gtgctctcct
3781 ctcacatcgc gacggggcta aagtgcatct cggcacccgc ccaacagaga aacagtacga
3841 aaccctggaa aatcagctcg cgttcctgtg tcagcaaggc ttctccctgg agaacgcact
3901 gtacgctctg tccgccgtgg gccactttac actgggctgc gtattggagg aacaggagca
3961 tcaagtagca aaagaggaaa gagagacacc taccaccgat tctatgcccc cacttctgag
4021 acaagcaatt gagctgttcg accggcaggg agccgaacct gccttccttt tcggcctgga
4081 actaatcata tgtggcctgg agaaacagct aaagtgcgaa agcggcgggc cgaccgacgc
4141 ccttgacgat tttgacttag acatgctccc agccgatgcc cttgacgact ttgaccttga
4201 tatgctgcct gctgacgctc ttgacgattt tgaccttgac atgctccccg ggtaactaag
4261 taaggatcga tccaagatat cgtattctta actatgttgc tccttttacg ctatgtggat
4321 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
4381 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
4441 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
4501 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
4561 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
4621 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga
4681 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
4741 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
4801 gtcggatctc cctttgggcc gcctccccgc ctgtttcgcc tcggcgtccg gactagaggt
4861 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
4921 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac
4981 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
5041 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
5101 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
5161 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
5221 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
5281 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
5341 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg
5401 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
5461 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
5521 tttggaggcc tttcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
5581 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
5641 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
5701 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
5761 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
5821 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
5881 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
5941 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
6001 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
6061 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
6121 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
6181 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
6241 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
6301 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
6361 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
6421 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
6481 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
6541 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
6601 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
6661 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
6721 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
6781 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
6841 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
6901 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
6961 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
7021 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
7081 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
7141 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
7201 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
7261 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
7321 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
7381 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
7441 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
7501 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
7561 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
7621 gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg
7681 caagcttaat gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt
7741 agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt
7801 ggtacgatcg tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca
7861 ctgaattgcc gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt
7921 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
7981 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
8041 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg
8101 gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac
8161 tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa
8221 aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg
8281 ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata
8341 taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg
8401 cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac catcccttca
8461 gacaggatca gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca
8521 tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa
8581 caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata
8641 tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag
8701 gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa
8761 taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa
8821 tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt
8881 tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc
8941 agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga
9001 tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga
9061 gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa
9121 ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa
9181 agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca
9241 taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt
9301 taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat
9361 tatcgtttca gacccacctc ccaaccccga ggggac
//
