| 质粒名称: | FU-tet-o-hKLF4 |
|---|---|
| 出品公司： | addgene |
| 目录编号: | 19775 |
| 存储实验室: | Konrad Hochedlinger |
|
载体骨架基本信息 |
|
| 载体名称: | FUdeltaGW |
| 空载体大小: | 8400bp |
| 完整质粒大小: | 9895 bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | c-myc |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 1320bp |
| 启动子: | T58A |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | EcoRI(未破坏) |
| 3’克隆位点: | EcoRI (未破坏) |
| 5’测序引物 | LNCX |
| 3’测序引物 | WPRE-R |
|
文献引用方法 |
|
| 材料和方法部分: | FU-tet-o-hc-myc was a gift from Konrad Hochedlinger (Addgene plasmid # 19775 ; http://n2t.net/addgene:19775 ; RRID:Addgene_19775) |
| 参考文献部分: | A high-efficiency system for the generation and study of human induced pluripotent stem cells. Maherali N, Ahfeldt T, Rigamonti A, Utikal J, Cowan C, Hochedlinger K. Cell Stem Cell. 2008 Sep 11. 3(3):340-5. 10.1016/j.stem.2008.08.003 PubMed 18786420 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS FU-tet-o-hc-myc 9895 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS FU-tet-o-hc-myc
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9895)
AUTHORS Maherali N, Ahfeldt T, Rigamonti A, Utikal J, Cowan C, Hochedlinger
K
TITLE A high-efficiency system for the generation and study of human
induced pluripotent stem cells.
JOURNAL Cell Stem Cell. 2008 Sep 11. 3(3):340-5.
PUBMED 18786420
REFERENCE 2 (bases 1 to 9895)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..9895
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(47..66)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 238..617
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 619..817
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 772..792
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
LTR 835..1015
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 1062..1187
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1680..1913
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 2098..2142
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 2440..2557
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
protein_bind 2610..2880
/label=tetracycline response element
/bound_moiety="tetracycline repressor TetR"
/note="contains seven copies of the tetracycline operator
tetO"
promoter 2913..2950
/label=minimal CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 2947..2971
/label=LNCX
/note="Human CMV promoter, forward primer"
regulatory 3074..3083
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 3089..3115
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 3134..3160
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 3179..3205
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 3227..4543
/codon_start=1
/product="human c-Myc proto-oncogene"
/label=c-Myc
/translation="PLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAPS
EDIWKKFELLPAPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTEL
LGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPNP
ARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDSL
LSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGSP
SAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNRK
CTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKAT
AYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA"
misc_feature 4587..5175
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(4640..4660)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(5058..5069)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(5178..5194)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5179..5195)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 5700..5880
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
primer_bind complement(5906..5923)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 5912..6136
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 6182..6610
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6269..6288)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 6479..6500
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(6619..6639)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 6624..6953
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 6804..6939
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 6866..6885
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
promoter 7001..7048
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 7067..7441
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
polyA_signal 7571..7692
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(7608..7627)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 7662..7681
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(7741..7757)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7741..7757)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(7754..7776)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 7765..7781
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7789..7819)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7834..7855
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(7972..7989)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(8143..8731)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8223..8242)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(8902..9762)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9525..9544
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9763..9867)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
2581 agatccagtt tggttatcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
2641 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
2701 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
2761 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
2821 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
2881 aaagtgaaag tcgagctcgg tacccgggtc gagtaggcgt gtacggtggg aggcctatat
2941 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
3001 cctccataga agacaccggg accgatccag cctccgcggc cccgaattga tcttcggcca
3061 gcgactagta gaagccacca tggatagcta tccttatgac gtgcctgact atgccagcct
3121 gatgctgatg agctatcctt atgacgtgcc tgactatgcc agcctgatgc tgatgagcta
3181 tccttatgac gtgcctgact atgccagcct gatgctgatg tctagacccc tcaacgttag
3241 cttcaccaac aggaactatg acctcgacta cgactcggtg cagccgtatt tctactgcga
3301 cgaggaggag aacttctacc agcagcagca gcagagcgag ctgcagcccc cggcgcccag
3361 cgaggatatc tggaagaaat tcgagctgct gcccgcgccg cccctgtccc ctagccgccg
3421 ctccgggctc tgctcgccct cctacgttgc ggtcacaccc ttctcccttc ggggagacaa
3481 cgacggcggt ggcgggagct tctccacggc cgaccagctg gagatggtga ccgagctgct
3541 gggaggagac atggtgaacc agagtttcat ctgcgacccg gacgacgaga ccttcatcaa
3601 aaacatcatc atccaggact gtatgtggag cggcttctcg gccgccgcca agctcgtctc
3661 agagaagctg gcctcctacc aggctgcgcg caaagacagc ggcagcccga accccgcccg
3721 cggccacagc gtctgctcca cctccagctt gtacctgcag gatctgagcg ccgccgcctc
3781 agagtgcatc gacccctcgg tggtcttccc ctaccctctc aacgacagca gctcgcccaa
3841 gtcctgcgcc tcgcaagact ccagcgcctt ctctccgtcc tcggattctc tgctctcctc
3901 gacggagtcc tccccgcagg gcagccccga gcccctggtg ctccatgagg agacaccgcc
3961 caccaccagc agcgactctg aggaggaaca agaagatgag gaagaaatcg atgttgtttc
4021 tgtggaaaag aggcaggctc ctggcaaaag gtcagagtct ggatcacctt ctgctggagg
4081 ccacagcaaa cctcctcaca gcccactggt cctcaagagg tgccacgtct ccacacatca
4141 gcacaactac gcagcgcctc cctccactcg gaaggactat cctgctgcca agagggtcaa
4201 gttggacagt gtcagagtcc tgagacagat cagcaacaac cgaaaatgca ccagccccag
4261 gtcctcggac accgaggaga atgtcaagag gcgaacacac aacgtcttgg agcgccagag
4321 gaggaacgag ctaaaacgga gcttttttgc cctgcgtgac cagatcccgg agttggaaaa
4381 caatgaaaag gcccccaagg tagttatcct taaaaaagcc acagcataca tcctgtccgt
4441 ccaagcagag gagcaaaagc tcatttctga agaggacttg ttgcggaaac gacgagaaca
4501 gttgaaacac aaacttgaac agctacggaa ctcttgtgcg taagtcgacg gatcctgaga
4561 attaattcga tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat
4621 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
4681 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
4741 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
4801 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
4861 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
4921 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
4981 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
5041 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
5101 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
5161 gggccgcctc cccgcatcga taccgtcgac ctcgagacct agaaaaacat ggagcaatca
5221 caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg
5281 aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag
5341 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
5401 aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
5461 ggcagaacta cacaccaggg ccagggatca gatatccact gacctttgga tggtgctaca
5521 agctagtacc agttgagcaa gagaaggtag aagaagccaa tgaaggagag aacacccgct
5581 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagta ttagagtgga
5641 ggtttgacag ccgcctagca tttcatcaca tggcccgaga gctgcatccg gactgtactg
5701 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
5761 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
5821 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
5881 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
5941 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
6001 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
6061 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
6121 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
6181 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
6241 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
6301 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
6361 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
6421 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
6481 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
6541 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
6601 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
6661 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
6721 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
6781 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
6841 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg
6901 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
6961 cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc
7021 atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagttgac
7081 cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga
7141 ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga
7201 cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg
7261 ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa
7321 cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga
7381 gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg
7441 acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
7501 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
7561 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
7621 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
7681 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
7741 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
7801 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
7861 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
7921 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
7981 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
8041 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
8101 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
8161 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
8221 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
8281 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
8341 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
8401 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
8461 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
8521 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
8581 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
8641 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
8701 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
8761 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
8821 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
8881 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
8941 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
9001 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
9061 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
9121 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
9181 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
9241 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
9301 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
9361 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
9421 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
9481 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
9541 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
9601 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
9661 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
9721 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
9781 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
9841 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgac
//
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