FU-tet-o-hKLF4

LOCUS       FU-tet-o-hKLF4                9801 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    FU-tet-o-hKLF4
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9801)
  AUTHORS   Maherali N, Ahfeldt T, Rigamonti A, Utikal J, Cowan C, Hochedlinger 
            K
  TITLE     A high-efficiency system for the generation and study of human 
            induced pluripotent stem cells.
  JOURNAL   Cell Stem Cell. 2008 Sep 11. 3(3):340-5.
  PUBMED    18786420
REFERENCE   2  (bases 1 to 9801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..9801
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2610..2880
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2913..2950
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2947..2971
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      3050..3059
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     misc_feature    4493..5081
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4546..4566)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4964..4975)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5084..5100)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5085..5101)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5606..5786
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5812..5829)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5818..6042
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6088..6516
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6175..6194)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6385..6406
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6525..6545)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6530..6859
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6710..6845
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6772..6791
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6907..6954
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6973..7347
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7477..7598
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7514..7533)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7568..7587
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7647..7663)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7647..7663)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7660..7682)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7671..7687
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7695..7725)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7740..7761
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7878..7895)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8049..8637)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8129..8148)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8808..9668)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9431..9450
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9669..9773)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttatcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
     2641 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
     2701 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
     2761 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     2821 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     2881 aaagtgaaag tcgagctcgg tacccgggtc gagtaggcgt gtacggtggg aggcctatat
     2941 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     3001 cctccataga agacaccggg accgatccag cctccgcggc cccgaattcg ccaccatggc
     3061 tgtcagcgac gcgctgctcc catctttctc cacgttcgcg tctggcccgg cgggaaggga
     3121 gaagacactg cgtcaagcag gtgccccgaa taaccgctgg cgggaggagc tctcccacat
     3181 gaagcgactt cccccagtgc ttcccggccg cccctatgac ctggcggcgg cgaccgtggc
     3241 cacagacctg gagagcggcg gagccggtgc ggcttgcggc ggtagcaacc tggcgcccct
     3301 acctcggaga gagaccgagg agttcaacga tctcctggac ctggacttta ttctctccaa
     3361 ttcgctgacc catcctccgg agtcagtggc cgccaccgtg tcctcgtcag cgtcagcctc
     3421 ctcttcgtcg tcgccgtcga gcagcggccc tgccagcgcg ccctccacct gcagcttcac
     3481 ctatccgatc cgggccggga acgacccggg cgtggcgccg ggcggcacgg gcggaggcct
     3541 cctctatggc agggagtccg ctccccctcc gacggctccc ttcaacctgg cggacatcaa
     3601 cgacgtgagc ccctcgggcg gcttcgtggc cgagctcctg cggccagaat tggacccggt
     3661 gtacattccg ccgcagcagc cgcagccgcc aggtggcggg ctgatgggca agttcgtgct
     3721 gaaggcgtcg ctgagcgccc ctggcagcga gtacggcagc ccgtcggtca tcagcgtcag
     3781 caaaggcagc cctgacggca gccacccggt ggtggtggcg ccctacaacg gcgggccgcc
     3841 gcgcacgtgc cccaagatca agcaggaggc ggtctcttcg tgcacccact tgggcgctgg
     3901 accccctctc agcaatggcc accggccggc tgcacacgac ttccccctgg ggcggcagct
     3961 ccccagcagg actaccccga ccctgggtct tgaggaagtg ctgagcagca gggactgtca
     4021 ccctgccctg ccgcttcctc ccggcttcca tccccacccg gggcccaatt acccatcctt
     4081 cctgcccgat cagatgcagc cgcaagtccc gccgctccat taccaagagc tcatgccacc
     4141 cggttcctgc atgccagagg agcccaagcc aaagagggga agacgatcgt ggccccggaa
     4201 aaggaccgcc acccacactt gtgattacgc gggctgcggc aaaacctaca caaagagttc
     4261 ccatctcaag gcacacctgc gaacccacac aggtgagaaa ccttaccact gtgactggga
     4321 cggctgtgga tggaaattcg cccgctcaga tgaactgacc aggcactacc gtaaacacac
     4381 ggggcaccgc ccgttccagt gccaaaaatg cgaccgagca ttttccaggt cggaccacct
     4441 cgccttacac atgaagaggc atttttaaga attcgatatc aagcttatcg ataatcaacc
     4501 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     4561 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     4621 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     4681 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     4741 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     4801 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     4861 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
     4921 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     4981 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     5041 ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacctcg
     5101 agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg
     5161 tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc
     5221 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     5281 gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta
     5341 ccacacacaa ggctacttcc ctgattggca gaactacaca ccagggccag ggatcagata
     5401 tccactgacc tttggatggt gctacaagct agtaccagtt gagcaagaga aggtagaaga
     5461 agccaatgaa ggagagaaca cccgcttgtt acaccctgtg agcctgcatg ggatggatga
     5521 cccggagaga gaagtattag agtggaggtt tgacagccgc ctagcatttc atcacatggc
     5581 ccgagagctg catccggact gtactgggtc tctctggtta gaccagatct gagcctggga
     5641 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     5701 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     5761 ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac ccgctgatca gcctcgactg
     5821 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     5881 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     5941 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     6001 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
     6061 ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg
     6121 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     6181 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     6241 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     6301 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     6361 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     6421 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     6481 aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt
     6541 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     6601 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     6661 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     6721 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     6781 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
     6841 aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca
     6901 gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg
     6961 aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc
     7021 gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt cgtggaggac
     7081 gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt ccaggaccag
     7141 gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc
     7201 gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc catgaccgag
     7261 atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg caactgcgtg
     7321 cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc caccgccgcc
     7381 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     7441 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
     7501 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     7561 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
     7621 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     7681 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     7741 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     7801 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     7861 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     7921 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     7981 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     8041 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     8101 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     8161 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     8221 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     8281 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     8341 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     8401 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     8461 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     8521 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     8581 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     8641 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     8701 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     8761 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     8821 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     8881 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     8941 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     9001 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     9061 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     9121 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     9181 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     9241 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     9301 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     9361 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     9421 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     9481 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     9541 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     9601 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     9661 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     9721 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     9781 ccccgaaaag tgccacctga c
//