| 质粒名称: | pHIV-iRFP720-E2A-Luc |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 104587 |
| 存储实验室: | Antonius Plagge |
|
载体骨架基本信息 |
|
| 载体名称: | pHIV-Luciferase |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，慢病毒，荧光素酶 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | iRFP720-E2A-Luciferase |
| 物种来源: | firefly |
| 插入大小: | 2661 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | bi-cistronic（插入件上的C端子） |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pHIV-iRFP720-E2A-Luc was a gift from Antonius Plagge (Addgene plasmid # 104587 ; http://n2t.net/addgene:104587 ; RRID:Addgene_104587) |
| 参考文献部分: | Multimodal cell tracking from systemic administration to tumour growth by combining gold nanorods and reporter genes. Comenge J, Sharkey J, Fragueiro O, Wilm B, Brust M, Murray P, Levy R, Plagge A. Elife. 2018 Jun 27;7. pii: 33140. doi: 10.7554/eLife.33140. 10.7554/eLife.33140 PubMed 29949503 |
| 相关载体: |
pHIV-EGFP pHIV-ZsGreen pHIV-dTomato pHIV-H2BmRFP pHIV-Luciferase pHIV-Luc-ZsGreen pHIV-iRFP720-E2A-Luc |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pHIV-iRFP720-E2A-Luc 9050 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Lentivirus vector for bi-cistronic expression of iRFP720 and firefly
luciferase from EF1a promoter.
ACCESSION .
VERSION .
KEYWORDS pHIV-iRFP720-E2A-Luc
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9050)
AUTHORS Comenge J, Sharkey J, Fragueiro O, Wilm B, Brust M, Murray P, Levy
R, Plagge A
TITLE Multimodal cell tracking from systemic administration to tumour
growth by combining gold nanorods and reporter genes.
JOURNAL Elife. 2018 Jun 27;7. pii: 33140. doi: 10.7554/eLife.33140.
PUBMED 29949503
REFERENCE 2 (bases 1 to 9050)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9050
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 414..433
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(652..756)
/gene="bla"
/label=AmpR promoter
primer_bind complement(831..850)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 1022..1401
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 1403..1601
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 1556..1576
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
LTR 1619..1799
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 1846..1971
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 2468..2701
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 3228..3345
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 3408..4586
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 3639..4577
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 4534..4554
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
regulatory 4645..4654
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 4651..5598
/codon_start=1
/product="near-infrared fluorescent protein with an
emission peak at 720 nm, engineered from a bacterial
phytochrome (Shcherbakova and Verkhusha, 2013)"
/label=iRFP720
/note="derived from Rhodopseudomonas palustris RpBphP2"
/translation="MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAGS
DNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAGFI
GSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVRKIT
GFDRVMIYRFASDFSGSVIAEDRCAEVESKLGLHYPASFIPAQARRLYTINPVRIIPDI
NYRPVPVTPDLNPVTGRPIDLSFAILRSVSPNHLEFMRNIGMHGTMSISILRGERLWGL
IVCHHRTPYYVDLDGRQACELVAQVLAWQIGVMEE"
CDS 5599..5658
/codon_start=1
/product="2A peptide from equine rhinitis A virus
polyprotein"
/label=E2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="QCTNYALLKLAGDVESNPGP"
CDS 5659..7311
/codon_start=1
/product="firefly luciferase"
/label=luciferase
/note="synthetic luc2 version of the luciferase gene"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
primer_bind complement(7314..7330)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7315..7331)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 7840..8020
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
rep_origin complement(8082..8670)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8162..8181)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(8841..651)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
ORIGIN
1 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
61 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
121 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
181 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
241 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
301 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
361 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
421 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
481 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
541 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
601 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
661 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
721 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
781 tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc
841 tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag
901 tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag
961 aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt
1021 tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
1081 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
1141 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
1201 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
1261 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
1321 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
1381 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
1441 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
1501 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
1561 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg cctgtactgg
1621 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
1681 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
1741 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
1801 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
1861 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
1921 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
1981 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
2041 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
2101 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
2161 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
2221 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
2281 aacaaaagta agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag
2341 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
2401 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
2461 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
2521 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
2581 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
2641 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
2701 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
2761 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
2821 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
2881 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
2941 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
3001 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
3061 caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa
3121 tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg aacggatcgg
3181 cactgcgtgc gccaattctg cagacaaatg gcagtattca tccacaattt taaaagaaaa
3241 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
3301 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
3361 gacagcagag atccagtttg gttagtaccg ggcccgctct agcgtgaggc tccggtgccc
3421 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
3481 attgaaccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
3541 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
3601 acgttctttt tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc
3661 gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc
3721 tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc
3781 cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg
3841 gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc
3901 tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg
3961 taaatgcggg ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg
4021 gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga
4081 gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc
4141 cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg
4201 aaagatggcc gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg
4261 gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg
4321 cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct
4381 tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca
4441 ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat
4501 ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt
4561 tttttcttcc atttcaggtg tcgtgagcgg ccgctgaact gaattctgca gtcgacggta
4621 ccgcgggccc gggatccacc ggtcgccacc atggcggaag gatccgtcgc caggcagcct
4681 gacctcttga cctgcgacga tgagccgatc catatccccg gtgccatcca accgcatgga
4741 ctgctgctcg ccctcgccgc cgacatgacg atcgttgccg gcagcgacaa ccttcccgaa
4801 ctcaccggac tggcgatcgg cgccctgatc ggccgctctg cggccgatgt cttcgactcg
4861 gagacgcaca accgtctgac gatcgccttg gccgagcccg gggcggccgt cggagcaccg
4921 atcactgtcg gcttcacgat gcgaaaggac gcaggcttca tcggctcctg gcatcgccat
4981 gatcagctca tcttcctcga gctcgagcct ccccagcggg acgtcgccga gccgcaggcg
5041 ttcttccgcc gcaccaacag cgccatccgc cgcctgcagg ccgccgaaac cttggaaagc
5101 gcctgcgccg ccgcggcgca agaggtgcgg aagattaccg gcttcgatcg ggtgatgatc
5161 tatcgcttcg cctccgactt cagcgggtcc gtgatcgcag aggatcggtg cgccgaggtc
5221 gagtcaaaac taggcctgca ctatcctgcc tcattcatcc cggcgcaggc ccgtcggctc
5281 tataccatca acccggtacg gatcattccc gatatcaatt atcggccggt gccggtcacc
5341 ccagacctca atccggtcac cgggcggccg attgatctta gcttcgccat cctgcgcagc
5401 gtctcgccca accatctgga gttcatgcgc aacataggca tgcacggcac gatgtcgatc
5461 tcgattttgc gcggcgagcg actgtgggga ttgatcgttt gccatcaccg aacgccgtac
5521 tacgtcgatc tcgatggccg ccaagcctgc gagctagtcg cccaggttct ggcctggcag
5581 atcggcgtga tggaagagca atgtacaaac tacgctttgt tgaaactcgc tggcgatgtt
5641 gaaagtaacc ccggtcctat ggaagatgcc aaaaacatta agaagggccc agcgccattc
5701 tacccactcg aagacgggac cgccggcgag cagctgcaca aagccatgaa gcgctacgcc
5761 ctggtgcccg gcaccatcgc ctttaccgac gcacatatcg aggtggacat tacctacgcc
5821 gagtacttcg agatgagcgt tcggctggca gaagctatga agcgctatgg gctgaataca
5881 aaccatcgga tcgtggtgtg cagcgagaat agcttgcagt tcttcatgcc cgtgttgggt
5941 gccctgttca tcggtgtggc tgtggcccca gctaacgaca tctacaacga gcgcgagctg
6001 ctgaacagca tgggcatcag ccagcccacc gtcgtattcg tgagcaagaa agggctgcaa
6061 aagatcctca acgtgcaaaa gaagctaccg atcatacaaa agatcatcat catggatagc
6121 aagaccgact accagggctt ccaaagcatg tacaccttcg tgacttccca tttgccaccc
6181 ggcttcaacg agtacgactt cgtgcccgag agcttcgacc gggacaaaac catcgccctg
6241 atcatgaaca gtagtggcag taccggattg cccaagggcg tagccctacc gcaccgcacc
6301 gcttgtgtcc gattcagtca tgcccgcgac cccatcttcg gcaaccagat catccccgac
6361 accgctatcc tcagcgtggt gccatttcac cacggcttcg gcatgttcac cacgctgggc
6421 tacttgatct gcggctttcg ggtcgtgctc atgtaccgct tcgaggagga gctattcttg
6481 cgcagcttgc aagactataa gattcaatct gccctgctgg tgcccacact atttagcttc
6541 ttcgctaaga gcactctcat cgacaagtac gacctaagca acttgcacga gatcgccagc
6601 ggcggggcgc cgctcagcaa ggaggtaggt gaggccgtgg ccaaacgctt ccacctacca
6661 ggcatccgcc agggctacgg cctgacagaa acaaccagcg ccattctgat cacccccgaa
6721 ggggacgaca agcctggcgc agtaggcaag gtggtgccct tcttcgaggc taaggtggtg
6781 gacttggaca ccggtaagac actgggtgtg aaccagcgcg gcgagctgtg cgtccgtggc
6841 cccatgatca tgagcggcta cgttaacaac cccgaggcta caaacgctct catcgacaag
6901 gacggctggc tgcacagcgg cgacatcgcc tactgggacg aggacgagca cttcttcatc
6961 gtggaccggc tgaagagcct gatcaaatac aagggctacc aggtagcccc agccgaactg
7021 gagagcatcc tgctgcaaca ccccaacatc ttcgacgccg gggtcgccgg cctgcccgac
7081 gacgatgccg gcgagctgcc cgccgcagtc gtcgtgctgg aacacggtaa aaccatgacc
7141 gagaaggaga tcgtggacta tgtggccagc caggttacaa ccgccaagaa gctgcgcggt
7201 ggtgttgtgt tcgtggacga ggtgcctaaa ggactgaccg gcaagttgga cgcccgcaag
7261 atccgcgaga ttctcattaa ggccaagaag ggcggcaaga tcgccgtgta aatcgatacc
7321 gtcgacctcg atcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct
7381 accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc
7441 acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
7501 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatatcctt
7561 gatctgtgga tctaccacac acaaggctac ttccctgatt ggcagaacta cacaccaggg
7621 ccagggatca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcaa
7681 gagaaggtag aagaagccaa tgaaggagag aacacccgct tgttacaccc tgtgagcctg
7741 catgggatgg atgacccgga gagagaagta ttagagtgga ggtttgacag ccgcctagca
7801 tttcatcaca tggcccgaga gctgcatccg gactgtactg ggtctctctg gttagaccag
7861 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
7921 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
7981 tccctcagac ccttttagtc agtgtggaaa atctctagca gcatgtgagc aaaaggccag
8041 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
8101 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
8161 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
8221 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
8281 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
8341 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
8401 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
8461 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
8521 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
8581 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
8641 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
8701 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
8761 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
8821 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
8881 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
8941 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
9001 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
//
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