| 质粒名称: | pHIV-Zsgreen |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 18121 |
| 存储实验室: | Bryan Welm, Zena Werb |
|
载体骨架基本信息 |
|
| 载体名称: | pSICO |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 6900 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，慢病毒 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Zsgreen,Zoanthus sp. green fluorescent protein |
| 物种来源: | Anthozoa |
| 插入大小: | 700 bp |
| 基因突变: | -- |
| GenBank编号 | AF168422 |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | MscI(未破坏) |
| 3' 克隆位点: | ClaI (未破坏) |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | |
| 参考文献部分: | Lentiviral transduction of mammary stem cells for analysis of gene function during development and cancer. Welm BE, Dijkgraaf GJ, Bledau AS, Welm AL, Werb Z. Cell Stem Cell. 2008 Jan 10. 2(1):90-102. 10.1016/j.stem.2007.10.002 PubMed 18371425 |
| 相关载体: |
pHIV-EGFP pHIV-ZsGreen pHIV-dTomato pHIV-H2BmRFP pHIV-Luciferase pHIV-Luc-ZsGreen pHIV-iRFP720-E2A-Luc |
买家导航
pHIVZsgreen载体基本信息
pHIVZsgreen载体图谱质粒图谱和多克隆位点信息
pHIVZsgreen载体简介
pHIVZsgreen载体序列
LOCUS pHIV-Zsgreen 7679 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pHIV-Zsgreen
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7679)
AUTHORS Welm BE, Dijkgraaf GJ, Bledau AS, Welm AL, Werb Z
TITLE Lentiviral transduction of mammary stem cells for analysis of gene
function during development and cancer.
JOURNAL Cell Stem Cell. 2008 Jan 10. 2(1):90-102.
PUBMED 18371425
REFERENCE 2 (bases 1 to 7679)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..7679
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(47..66)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 238..617
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 619..817
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 772..792
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
LTR 835..1015
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 1062..1187
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1684..1917
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 2444..2561
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2624..3802
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 2855..3793
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 3750..3770
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
misc_feature 3863..4449
/label=IRES2
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(4030..4047)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 4257..4276
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 4450..5145
/codon_start=1
/product="Zoanthus green fluorescent protein"
/label=ZsGreen1
/note="mammalian codon-optimized"
/translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV
CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG
DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
AIASGSALP"
primer_bind complement(5159..5175)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5160..5176)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 5685..5865
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
rep_origin complement(5927..6515)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6007..6026)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(6686..7546)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7309..7328
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7547..7651)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1501 aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga
1561 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
1621 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
1681 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
1741 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
1801 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
1861 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
1921 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
1981 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
2041 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
2101 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
2161 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
2221 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
2281 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
2341 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact
2401 gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg
2461 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
2521 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
2581 gcagagatcc agtttggtta gtaccgggcc cgctctagcg tgaggctccg gtgcccgtca
2641 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
2701 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
2761 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
2821 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg
2881 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca
2941 gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg
3001 cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg
3061 ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc
3121 catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa
3181 tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc
3241 ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat
3301 cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg
3361 tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag
3421 atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga
3481 gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc
3541 atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg
3601 gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga
3661 gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc
3721 cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt
3781 tcttccattt caggtgtcgt gagcggccgc tgaactgaat tcatcgacgt taactattct
3841 agagtacccg ggctaggatc cgcccctctc cctccccccc ccctaacgtt actggccgaa
3901 gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt
3961 cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg
4021 gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc
4081 ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc
4141 ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa
4201 aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc
4261 tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg
4321 gatctgatct ggggcctcgg tacacatgct ttacatgtgt ttagtcgagg ttaaaaaaac
4381 gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataatatg
4441 gccacaacca tggcccagtc caagcacggc ctgaccaagg agatgaccat gaagtaccgc
4501 atggagggct gcgtggacgg ccacaagttc gtgatcaccg gcgagggcat cggctacccc
4561 ttcaagggca agcaggccat caacctgtgc gtggtggagg gcggcccctt gcccttcgcc
4621 gaggacatct tgtccgccgc cttcatgtac ggcaaccgcg tgttcaccga gtacccccag
4681 gacatcgtcg actacttcaa gaactcctgc cccgccggct acacctggga ccgctccttc
4741 ctgttcgagg acggcgccgt gtgcatctgc aacgccgaca tcaccgtgag cgtggaggag
4801 aactgcatgt accacgagtc caagttctac ggcgtgaact tccccgccga cggccccgtg
4861 atgaagaaga tgaccgacaa ctgggagccc tcctgcgaga agatcatccc cgtgcccaag
4921 cagggcatct tgaagggcga cgtgagcatg tacctgctgc tgaaggacgg tggccgcttg
4981 cgctgccagt tcgacaccgt gtacaaggcc aagtccgtgc cccgcaagat gcccgactgg
5041 cacttcatcc agcacaagct gacccgcgag gaccgcagcg acgccaagaa ccagaagtgg
5101 cacctgaccg agcacgccat cgcctccggc tccgccttgc cctaaggatc ggccgcatcg
5161 ataccgtcga cctcgatcga gacctagaaa aacatggagc aatcacaagt agcaatacag
5221 cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc
5281 cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
5341 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata
5401 tccttgatct gtggatctac cacacacaag gctacttccc tgattggcag aactacacac
5461 cagggccagg gatcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
5521 agcaagagaa ggtagaagaa gccaatgaag gagagaacac ccgcttgtta caccctgtga
5581 gcctgcatgg gatggatgac ccggagagag aagtattaga gtggaggttt gacagccgcc
5641 tagcatttca tcacatggcc cgagagctgc atccggactg tactgggtct ctctggttag
5701 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
5761 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
5821 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagcatg tgagcaaaag
5881 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
5941 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
6001 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
6061 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
6121 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
6181 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
6241 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
6301 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
6361 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
6421 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
6481 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
6541 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
6601 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
6661 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
6721 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
6781 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
6841 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
6901 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
6961 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
7021 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
7081 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
7141 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
7201 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
7261 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
7321 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
7381 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
7441 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
7501 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
7561 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
7621 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgac
//
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pHIVZsgreen载体质粒应用举例
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