质粒名称: | pHIV-dTomato |
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出品公司: | 爱迪基因 |
目录编号: | 21374 |
存储实验室: | Bryan Welm |
载体骨架基本信息 |
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载体名称: | pSICO |
载体抗性: | 氨苄青霉素 |
空载体大小: | 6900 bp |
完整质粒大小: | 7680bp |
载体类型: | 哺乳动物表达,慢病毒 |
高拷贝/低拷贝: | 高拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
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克隆方法: | 限制性内切酶 |
5' 测序引物 | 5'-TGGAATTTGCCCTTTTTGAG-3' |
3' 测序引物 | 5'-AGGAACTGCTTCCTTCACGA-3' |
文献引用方法 |
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材料和方法部分: | pHIV-dTomato was a gift from Bryan Welm (Addgene plasmid # 21374 ; http://n2t.net/addgene:21374 ; RRID:Addgene_21374) |
相关载体: |
pHIV-EGFP pHIV-ZsGreen pHIV-dTomato pHIV-H2BmRFP pHIV-Luciferase pHIV-Luc-ZsGreen pHIV-iRFP720-E2A-Luc |
买家导航
pHIVdTomato载体基本信息
pHIVdTomato载体图谱质粒图谱和多克隆位点信息
pHIVdTomato载体简介
pHIVdTomato载体序列
LOCUS pHIV-dTomato 7680 bp ds-DNA circular SYN 23-APR-2020 DEFINITION self-inactivating lentiviral plasmid for co-expression of your gene of interest and dTomato. ACCESSION . VERSION . KEYWORDS pHIV-dTomato SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7680) TITLE pHIV-dTomato REFERENCE 2 (bases 1 to 7680) AUTHORS . TITLE Direct Submission JOURNAL Exported Apr 23, 2020 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7680 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(47..66) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 238..617 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 619..817 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 772..792 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" LTR 835..1015 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 1062..1187 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1684..1917 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." misc_feature 2444..2561 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2624..3802 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2855..3793 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3750..3770 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" misc_feature 3863..4449 /label=IRES2 /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(4030..4047) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 4257..4276 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 4450..5154 /codon_start=1 /product="dimeric variant of DsRed fluorescent protein (Shaner et al., 2004)" /label=dTomato /note="mammalian codon-optimized" /translation="MSVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQT AKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDG GLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEI HQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGR HHLFLYGMDELYK" primer_bind complement(4586..4604) /label=mCherry-R /note="mCherry, reverse primer" primer_bind 4854..4873 /label=mCherry-F /note="mCherry, forward primer" primer_bind 5048..5071 /label=DsRed1-C /note="DsRed1, forward primer" primer_bind complement(5160..5176) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(5161..5177) /label=pBluescriptKS /note="For pBluescript vector" LTR 5686..5866 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" rep_origin complement(5928..6516) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(6008..6027) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(6687..7547) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 7310..7329 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(7548..7652) /gene="bla" /label=AmpR promoter ORIGIN 1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1501 aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga 1561 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt 1621 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg 1681 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc 1741 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa 1801 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa 1861 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg 1921 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg 1981 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga 2041 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga 2101 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa 2161 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg 2221 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc 2281 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga 2341 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact 2401 gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg 2461 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa 2521 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca 2581 gcagagatcc agtttggtta gtaccgggcc cgctctagcg tgaggctccg gtgcccgtca 2641 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg 2701 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct 2761 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt 2821 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg 2881 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca 2941 gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg 3001 cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg 3061 ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc 3121 catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa 3181 tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc 3241 ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat 3301 cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg 3361 tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag 3421 atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga 3481 gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc 3541 atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg 3601 gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga 3661 gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc 3721 cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt 3781 tcttccattt caggtgtcgt gagcggccgc tgaactgaat tcatcgacgt taactattct 3841 agagtacccg ggctaggatc cgcccctctc cctccccccc ccctaacgtt actggccgaa 3901 gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt 3961 cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg 4021 gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc 4081 ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc 4141 ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa 4201 aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc 4261 tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg 4321 gatctgatct ggggcctcgg tacacatgct ttacatgtgt ttagtcgagg ttaaaaaaac 4381 gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataatatg 4441 gccacaacca tgtcagtgag caagggcgag gaggtcatca aagagttcat gcgcttcaag 4501 gtgcgcatgg agggctccat gaacggccac gagttcgaga tcgagggcga gggcgagggc 4561 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc 4621 ttcgcctggg acatcctgtc cccccagttc atgtacggct ccaaggcgta cgtgaagcac 4681 cccgccgaca tccccgatta caagaagctg tccttccccg agggcttcaa gtgggagcgc 4741 gtgatgaact tcgaggacgg cggtctggtg accgtgaccc aggactcctc cctgcaggac 4801 ggcacgctga tctacaaggt gaagatgcgc ggcaccaact tcccccccga cggccccgta 4861 atgcagaaga agaccatggg ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc 4921 gtgctgaagg gcgagatcca ccaggccctg aagctgaagg acggcggcca ctacctggtg 4981 gagttcaaga ccatctacat ggccaagaag cccgtgcaac tgcccggcta ctactacgtg 5041 gacaccaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag 5101 cgctccgagg gccgccacca cctgttcctg tacggcatgg acgagctgta caagtaaatc 5161 gataccgtcg acctcgatcg agacctagaa aaacatggag caatcacaag tagcaataca 5221 gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt 5281 ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc 5341 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat 5401 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca 5461 ccagggccag ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt 5521 gagcaagaga aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg 5581 agcctgcatg ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc 5641 ctagcatttc atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta 5701 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa 5761 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac 5821 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagcat gtgagcaaaa 5881 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 5941 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 6001 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 6061 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 6121 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 6181 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 6241 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 6301 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 6361 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 6421 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc 6481 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 6541 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 6601 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 6661 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 6721 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 6781 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca 6841 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 6901 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 6961 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca 7021 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca 7081 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga 7141 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact 7201 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga 7261 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg 7321 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc 7381 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga 7441 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat 7501 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt 7561 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt 7621 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac //
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pHIVdTomato载体质粒应用举例
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