| 质粒名称: | HA-p62 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 28027 |
| 存储实验室: | Qing Zhong |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA4/TO |
| 载体出品公司: | Invitrogen |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 5078 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 博莱霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | p62,SQSTM1,sequestosome |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 1070 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | HA（插入件上的C端子） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | EcoRI (未破坏) |
| 3' 克隆位点: | NotI (未破坏) |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | BGH-rev |
|
文献引用方法 |
|
| 材料和方法部分: | HA-p62 was a gift from Qing Zhong (Addgene plasmid # 28027 ; http://n2t.net/addgene:28027 ; RRID:Addgene_28027) |
| 参考文献部分: | Keap1 facilitates p62-mediated ubiquitin aggregate clearance via autophagy. Fan W, Tang Z, Chen D, Moughon D, Ding X, Chen S, Zhu M, Zhong Q. Autophagy. 2010 Jul 22. 6(5):. 10.4161/auto.6.5.12189 PubMed 20495340 |
买家导航
HAp62载体基本信息
HAp62载体图谱质粒图谱和多克隆位点信息
HAp62载体简介
HAp62载体序列
LOCUS HA-p62 6414 bp ds-DNA circular SYN 17-MAY-2019
DEFINITION Mammalian expression of HA tagged human p62.
ACCESSION .
VERSION .
KEYWORDS HA-p62
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6414)
AUTHORS Fan W, Tang Z, Chen D, Moughon D, Ding X, Chen S, Zhu M, Zhong Q
TITLE Keap1 facilitates p62-mediated ubiquitin aggregate clearance via
autophagy.
JOURNAL Autophagy. 2010 Jul 22. 6(5):.
PUBMED 20495340
REFERENCE 2 (bases 1 to 6414)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 17, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6414
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind 107..128
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 143..173
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 181..197
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 186..208
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 205..221
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 205..221
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
polyA_signal 270..391
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(281..300)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 335..354
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
CDS complement(521..895)
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
promoter complement(914..961)
/label=EM7 promoter
/note="synthetic bacterial promoter "
promoter complement(1009..1338)
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 1023..1158
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind complement(1077..1096)
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind 1323..1343
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin complement(1352..1780)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(1462..1483)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 1674..1693
/label=F1ori-R
/note="F1 origin, reverse primer"
polyA_signal 1826..2050
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind 2039..2056
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
CDS complement(2097..2123)
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
primer_bind complement(3585..3609)
/label=LNCX
/note="Human CMV promoter, forward primer"
protein_bind 3619..3637
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 3640..3658
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
promoter complement(3660..3863)
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind complement(3689..3709)
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
enhancer 3864..4243
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
primer_bind 4415..4434
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
promoter 4509..4613
/gene="bla"
/label=AmpR promoter
CDS 4614..5474
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(4832..4851)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 5645..6233
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 6134..6153
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 6387..6404
/label=L4440
/note="L4440 vector, forward primer"
ORIGIN
1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct
241 agctagaggt cgacggtata cagacatgat aagatacatt gatgagtttg gacaaaccac
301 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
361 tgtaaccatt ataagctgca ataaacaagt tggggtgggc gaagaactcc agcatgagat
421 ccccgcgctg gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt
481 catagaaggc ggcggtggaa tcgaaatctc gtagcacgtg tcagtcctgc tcctcggcca
541 cgaagtgcac gcagttgccg gccgggtcgc gcagggcgaa ctcccgcccc cacggctgct
601 cgccgatctc ggtcatggcc ggcccggagg cgtcccggaa gttcgtggac acgacctccg
661 accactcggc gtacagctcg tccaggccgc gcacccacac ccaggccagg gtgttgtccg
721 gcaccacctg gtcctggacc gcgctgatga acagggtcac gtcgtcccgg accacaccgg
781 cgaagtcgtc ctccacgaag tcccgggaga acccgagccg gtcggtccag aactcgaccg
841 ctccggcgac gtcgcgcgcg gtgagcaccg gaacggcact ggtcaacttg gccatggttt
901 agttcctcac cttgtcgtat tatactatgc cgatatacta tgccgatgat taattgtcaa
961 cacgtgctga tcagatccga aaatggatat acaagctccc gggagctttt tgcaaaagcc
1021 taggcctcca aaaaagcctc ctcactactt ctggaatagc tcagaggcag aggcggcctc
1081 ggcctctgca taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg
1141 cggagttagg ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag
1201 atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc
1261 tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc
1321 acaccctaac tgacacacat tccacagaat taattcgcgt taaatttttg ttaaatcagc
1381 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
1441 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
1501 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
1561 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
1621 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
1681 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
1741 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt ggggataccc cctagagccc
1801 cagctggttc tttccgcctc agaagccata gagcccaccg catccccagc atgcctgcta
1861 ttgtcttccc aatcctcccc cttgctgtcc tgccccaccc caccccccag aatagaatga
1921 cacctactca gacaatgcga tgcaatttcc tcattttatt aggaaaggac agtgggagtg
1981 gcaccttcca gggtcaagga aggcacgggg gaggggcaaa caacagatgg ctggcaacta
2041 gaaggcacag tcgaggctga tcagcgggtt taaacgggcc ctctagactc gagtcaagcg
2101 taatctggaa catcgtatgg gtagcggccg cgcaacggcg ggggatgctt tgaatactgg
2161 atggtgtcca gagccgctcc gatgtcatag ttcttggtct gcaggagcct ggtgagccag
2221 ccgccttcat cagagaagcc catggacagc atctgggaga gggactcaat cagccgcggg
2281 tcagcctctg gcgggagatg tgggtacaag gcagcttcct tcagccctgt gggtccctcc
2341 tgggaggggt ccagagagct tggcccttcg gattctggca tctgtaggga ctggagttca
2401 cctgtagacg ggtccacttc ttttgaagac agatgggtcc agtcatcatc tcctcctgaa
2461 cagttatccg actccatctg ttcctcaggg cgcccctcgg actccaaggc gatctttctc
2521 atctgctccg ccagagactg cgtggcgccc tcaacattcc cacccggctt gctgggatca
2581 gagcagcagc tgcttggctg tgagctgctc ttctcctctg tgctggaact ctctggagag
2641 acgggggtca ggcggcttct tttccctccg tgctccacat cgatatcaac ttcaatgccc
2701 agagggctaa gggcagctgc cacactctcc ccaacgttct tcaggaaatt cacactcgga
2761 tcctccgatg gaccagaagc tgattctgcc gtggggccag ggcgggcctc ccctgcacga
2821 ggaggacgtg ggctccagtt tcctggtgga cccatttccc atcctggcca cccgaagtgt
2881 ccgtgtttca ccttccggag ccagcggctg tgcgagaagc cctcagacag gtgcccgaag
2941 gggctgggga atgcgagctt ggtgtgcccc cggtgcaagc cctttccctc gcagacgcta
3001 cacaagtcgt agtctgggca gacgctgcac ttgtagcggg ttcctaccac aggcccattg
3061 cagccatcgc agatcacatt ggggtgcacc atgttgcggg gcgcctcctg agcacacggt
3121 gggcggtggt cccgccggca ctcttttttc tctttaatgt agattcggaa gatgtcatcc
3181 ttcacgtagg acatggccat tgtcaattcc tcgtcactgg aaaaggcaac caagtccccg
3241 tcctcatcgc ggtagtgcgc ctggaagccg ccaggccgca gcgcggggaa cagggcggcc
3301 acccggctca gcagccgctc gcagggtccc ggacccgccg cagcctcggc ttccgcctca
3361 ggctcggggc tgcagcagaa gctgaagcgg cgaatctcgc gcgccgcgtc ctccttgccc
3421 agaaggtagg ccttcacggt gagcgacgcc atagaattcc accacactgg actagtggat
3481 ccgagctcgg taccaagctt aagtttaaac gctagagtcc ggaggctgga tcggtcccgg
3541 tgtcttctat ggaggtcaaa acagcgtgga tggcgtctcc aggcgatctg acggttcact
3601 aaacgagctc gtcgacgatc tctatcactg atagggagat ctctatcact gatagggaga
3661 gctctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg
3721 gagttgttac gacattttgg aaagtcccgt tgattttggt gccaaaacaa actcccattg
3781 acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc acgcccattg
3841 atgtactgcc aaaaccgcat caccatggta atagcgatga ctaatacgta gatgtactgc
3901 caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg
3961 tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg
4021 gcagtttacc gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact
4081 atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc
4141 gggccattta ccgtaagtta tgtaacgcgg aactccatat atgggctatg aactaatgac
4201 cccgtaattg attactatta ataactagtc aataatcaat gtcaacgcgt atatctggcc
4261 cgtacatcgc gaagcagcgc aaaacgccta accctaagca gattcttcat gcaattgtcg
4321 gtcaagcctt gccttgttgt agcttaaatt ttgctcgcgc actactcagc gacctccaac
4381 acacaagcag ggagcagata ctggcttaac tatgcggcat cagagcagat tgtactgaga
4441 gtgcaccata ggggatcggg agatctcccg atccgtcgac gtcaggtggc acttttcggg
4501 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
4561 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
4621 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
4681 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
4741 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
4801 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
4861 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
4921 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
4981 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
5041 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
5101 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
5161 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
5221 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
5281 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
5341 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
5401 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
5461 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
5521 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
5581 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
5641 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
5701 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
5761 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
5821 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
5881 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
5941 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
6001 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
6061 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
6121 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
6181 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
6241 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
6301 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
6361 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaag
//
