| 质粒名称: | pHAGE2-TetOminiCMV-mCherry |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 136615 |
| 存储实验室: | Hans Schöler |
|
载体骨架基本信息 |
|
| 载体名称: | pHAGE2 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 6000 bp |
| 完整质粒大小: | 6966 bp |
| 载体修饰: | -- |
| 载体类型: | 细菌表达，慢病毒 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | mCherry |
| 物种来源: | -- |
| 插入大小: | 710 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pHAGE2-TetOminiCMV-mCherry was a gift from Hans Schöler (Addgene plasmid # 136615 ; http://n2t.net/addgene:136615 ; RRID:Addgene_136615) |
| 参考文献部分: | Excluding Oct4 from Yamanaka Cocktail Unleashes the Developmental Potential of iPSCs. Velychko S, Adachi K, Kim KP, Hou Y, MacCarthy CM, Wu G, Scholer HR. Cell Stem Cell. 2019 Oct 30. pii: S1934-5909(19)30423-0. doi: 10.1016/j.stem.2019.10.002. 10.1016/j.stem.2019.10.002 PubMed 31708402 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pHAGE2-TetOminiCMV-mCherry 6966 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Dox-inducible lentivral vector expressing mCherry red fluorescent
protein.
ACCESSION .
VERSION .
KEYWORDS pHAGE2-TetOminiCMV-mCherry
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6966)
AUTHORS Velychko S, Adachi K, Kim KP, Hou Y, MacCarthy CM, Wu G, Scholer HR
TITLE Excluding Oct4 from Yamanaka Cocktail Unleashes the Developmental
Potential of iPSCs.
JOURNAL Cell Stem Cell. 2019 Oct 30. pii: S1934-5909(19)30423-0. doi:
10.1016/j.stem.2019.10.002.
PUBMED 31708402
REFERENCE 2 (bases 1 to 6966)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6966
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind 2..272
/label=tetracycline response element
/bound_moiety="tetracycline repressor TetR"
/note="contains seven copies of the tetracycline operator
tetO"
primer_bind 340..364
/label=LNCX
/note="Human CMV promoter, forward primer"
CDS 435..1145
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
primer_bind complement(580..598)
/label=mCherry-R
/note="mCherry, reverse primer"
primer_bind 848..867
/label=mCherry-F
/note="mCherry, forward primer"
misc_feature 1159..1747
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(1212..1232)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(1630..1641)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 1895..2075
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
primer_bind complement(2122..2140)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 2208..2312
/gene="bla"
/label=AmpR promoter
CDS 2313..3173
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(2531..2550)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 3344..3932
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 3833..3852
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 4086..4103
/label=L4440
/note="L4440 vector, forward primer"
primer_bind 4195..4214
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
protein_bind 4343..4364
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 4379..4409
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4417..4433
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 4422..4444
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 4441..4457
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 4441..4457
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 4507..4526
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
LTR 4718..5351
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 5398..5523
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 6020..6253
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 6438..6482
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 6775..6892
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
ORIGIN
1 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
61 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt
121 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt
181 gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag
241 tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagctc ggtacccggg
301 tcgaggtagg catgtacggt gggaggccta tataagcaga gctcgtttag tgaaccgtca
361 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc
421 cagcctgcgg ccgcatggtg agcaagggcg aggaggataa catggccatc atcaaggagt
481 tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg
541 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
601 gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg
661 cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct
721 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact
781 cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct
841 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt
901 accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg
961 gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg
1021 gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg
1081 tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca
1141 agtaaatcga tagatcctaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
1201 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
1261 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
1321 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
1381 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
1441 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
1501 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
1561 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
1621 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
1681 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
1741 tccccgcctg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
1801 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatcacct
1861 gcaggacagg cgcgccctgc tttttgcttg tactgggtct ctctggttag accagatctg
1921 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
1981 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
2041 cagacccttt tagtcagtgt ggaaaatctc tagcacccgg gcgattaagg aaagggctag
2101 atcattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
2161 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
2221 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
2281 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
2341 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
2401 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
2461 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
2521 ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg
2581 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
2641 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
2701 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
2761 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
2821 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
2881 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
2941 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
3001 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
3061 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
3121 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
3181 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
3241 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
3301 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
3361 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
3421 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
3481 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
3541 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
3601 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
3661 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
3721 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
3781 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
3841 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
3901 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
3961 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
4021 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
4081 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
4141 ccgcgcgttg gccgattcat taatgcagca agctcatggc tgactaattt tttttattta
4201 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
4261 tggaggccta ggcttttgca aaaagctccc cgtggcacga caggtttccc gactggaaag
4321 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
4381 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
4441 caggaaacag ctatgacatg attacgaatt tcacaaataa agcatttttt tcactgcatt
4501 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa
4561 ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt
4621 acctgtggtt tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa
4681 attgtatttg ttaaatatgt actacaaact tagtagttgg aagggctaat tcactcccaa
4741 agaagacaag atatccttga tctgtggatc taccacacac aaggctactt ccctgattag
4801 cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag
4861 ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg
4921 ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg
4981 tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag
5041 aactgctgat atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt
5101 ggcctgggcg ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt
5161 tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
5221 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
5281 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
5341 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
5401 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
5461 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
5521 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
5581 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
5641 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
5701 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
5761 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
5821 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccggccg ctgatcttca
5881 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
5941 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
6001 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
6061 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
6121 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
6181 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
6241 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
6301 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
6361 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
6421 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
6481 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
6541 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
6601 ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca
6661 ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag
6721 tgaacggatc tcgacggtat cgccgaattc acaaatggca gtattcatcc acaattttaa
6781 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
6841 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta
6901 ttacagggac agcagagatc cagtttggac tagtccacac cctaactgac acactcgagt
6961 ttacca
//
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