| 质粒名称: | pCS2-UTX-F |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 17438 |
| 存储实验室: | pCS2+ NLSMT |
|
载体骨架基本信息 |
|
| 载体名称: | pN1 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 4400 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 未知 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | UTX,ubiquitously transcribed tetratricopeptide repeat, X chromosome |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 4200 bp |
| 基因突变: | -- |
| GenBank编号 | NM_021140 |
| 启动子 | -- |
| 标签/融合蛋白: |
xMyc（骨干网上的N个终端）
标志（插入时的C端子）
|
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | XhoI (未破坏) |
| 3' 克隆位点: | XbaI (未破坏) |
| 5' 测序引物 | SP6 |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pCS2-UTX-F was a gift from Kai Ge (Addgene plasmid # 17438 ; http://n2t.net/addgene:17438 ; RRID:Addgene_17438) |
| 参考文献部分: | Identification of JmjC domain-containing UTX and JMJD3 as histone H3 lysine 27 demethylases. Hong S, Cho YW, Yu LR, Yu H, Veenstra TD, Ge K. Proc Natl Acad Sci U S A. 2007 Nov 20. 104(47):18439-44. 10.1073/pnas.0707292104 PubMed 18003914 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCS2-UTX-F 8611 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Expression of UTX in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS pCS2-UTX-F
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8611)
AUTHORS Hong S, Cho YW, Yu LR, Yu H, Veenstra TD, Ge K
TITLE Identification of JmjC domain-containing UTX and JMJD3 as histone H3
lysine 27 demethylases.
JOURNAL Proc Natl Acad Sci U S A. 2007 Nov 20. 104(47):18439-44.
PUBMED 18003914
REFERENCE 2 (bases 1 to 8611)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8611
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind 55..76
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 91..121
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 129..145
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 134..156
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 153..169
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 153..169
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 188..208
/label=T3
/note="T3 promoter, forward primer"
promoter 190..208
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
polyA_signal 321..455
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(358..377)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 412..431
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
promoter 460..477
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS complement(489..512)
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
CDS complement(4734..4763)
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS complement(4797..4826)
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS complement(4836..4865)
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS complement(4875..4904)
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS complement(4914..4943)
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS complement(4953..4982)
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS complement(5001..5021)
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
regulatory 5024..5033
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
promoter complement(5065..5083)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(5066..5083)
/label=SP6
/note="SP6 promoter, forward primer"
promoter complement(5118..6095)
/label=CMV IE94 promoter
/note="enhancer/promoter region of simian cytomegalovirus
major immediate early transcription unit IE94"
rep_origin 6173..6628
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6260..6279)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 6470..6491
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 6654..6758
/gene="bla"
/label=AmpR promoter
CDS 6759..7619
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(6977..6996)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 7790..8378
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 8279..8298
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 8532..8549
/label=L4440
/note="L4440 vector, forward primer"
ORIGIN
1 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
61 gagttagctc actcattagg caccccaggc tttacacttt atgctcccgg ctcgtatgtt
121 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
181 caagcgcgca attaaccctc actaaaggga acaaaagctg ggtaccgggc ccaatgcatt
241 ggcgccgcgg ccgcgaatta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
301 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
361 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
421 ccaaactcat caatgtatct tatcatgtct ggatctacgt aatacgactc actatagttc
481 tagaatcact tgtcatcgtc gtccttgtag tcagatgagg cggatggtaa tggaggagct
541 aatgtaaatt ggtcatagac ttgcatcagg tcctccattt tgtactgttc tagcaccaca
601 aagttttcca agtttccgct tgtttttcgt gcacaatctt ggcaatgtac tatgtaggtc
661 tttcgtgaat tactctcatt agtgacaaaa agcagatcaa aaacctccac ttcacaaatg
721 ctacagtaat gagctggttc tttttttgtc cgcccatgcc atataatctc ttttcctgca
781 gcaatgagag cttccctcaa tgtctgacat tgcttcagag ttcttagaag acaatactta
841 atcatttcaa aaagctttgg atctgagacc ttgatatttc gtgccatatt ccaggaaaga
901 tgaaccatgg gtactattga cttcacactt tgcaatttgt tccattcgta ccgttccact
961 gccaatttat actggcaggc tgtaagtgga ccaacattcc aagcaatgtt gttgcaccag
1021 ccaatagcct gaacccagtg aacagtgcct gcatttatcc agaccaaatc tccaggtcgc
1081 tgaataaacc tatacactgg aacatttgct tcataaagat cttcaagatt gggccaccaa
1141 gaacccatta ggaaattcaa attatttttt tcacagaagt cattcagaac accccagtaa
1201 ccttcaggaa caacaaacca ttcacagtca cctgggccaa tatttatgtt aactgaacag
1261 aagttgttat tttcctgatg acctggtgtt ctgctccctg gaactttcat gtatagttga
1321 actgtgttca tgcccaatat ggtatgacca acatggctta gaagatttcc tgctgatacg
1381 acacgcacaa aagcaggaag tttagtcagc tcatgtagct gcaacttcca ctttttgtca
1441 tcagataggt caatattggt cccaaacttt atggttttaa agggtccttt cctcctcctc
1501 ccagaattat ctgacgatgt agattcacta tctgagtggt ctttatgatg acttcttttt
1561 tcattttctt ctctcaatga ttcctggaat gaggaggcct ggtactgtgc atatttagca
1621 attgtagtat gagatctatt actttcacaa tgccagattt tctttgttcc agtggggtcc
1681 cagttttcat ctgctggctg caacaactgt gtcctcactt ctaccatatg ttcattgtta
1741 gcttccacca aagttttagt agagaaaagt cccaggtcta acttaagagc tccagcaagg
1801 ccacgtatta ctgtaacagg gttgttcgga tttgtacaaa attgatgtaa tggaggaaag
1861 aaagcatcac gtttattttc caagtaaata ctaggtgtag gtggattcaa cttgtccttt
1921 ggcaagggag ggtatggtga agatggtggt cttggaggtg gacatttatc caacaaaatg
1981 ctactgttag ataagccatt tttacctaga ttcctgcatg ccttcagaac ttctgctgag
2041 ctggggtata tggacacaga cattgatggt atgatctgtg gactaggttt gtggttaacc
2101 agaagcagat ctgttttcat ggggctctga gattcttcca tcccttctcc attaattgtg
2161 tgtagcccac tgtgagggct gttaaggctg gtaacactgt ttgtggttgt ctgctcagtg
2221 gattttggag aaggtgttgc tgttgaaatg gctgaagatg gtgaagaggc aacagagtta
2281 tcagtctttg tatgaacagc tgggtggatg ttattgactt tgtcacaggt tccagtaccc
2341 acattgttat tggcttttcc catcaacaag gcagagagct gaggattgtc tgaacttagt
2401 aaacctggtg acttagaatc tccatggcta ggactgcaaa cagcatctgc cgtcatctga
2461 tggacatgat taggaagtcc ctcgacactg gcagtgctgt taggtgtctc tccagtgtgc
2521 ctgcttgttt caggcacctt caatatgttt cctgaaggct tgctctcttt ggttaaggta
2581 atgccttgtt gtccacctga ggtagcagtg tgagaggaga ggtgattgag agcagccccc
2641 tgtgttactg aattgctagg cagggtggga tgtccgttct gattctgaat accagagcca
2701 gctgcctgga gatgctggga aggcccagtg gaagagagag gtcgttcacc attaggacct
2761 gccgaatgtg aactctgacc tttgtgaagc ccctgagtgg agttagatag ttggtttttc
2821 cacggctcct ctgcgctgct ggtcaggttt gtgcggttat gaggtagagt gagtgcgttt
2881 cgctgcaggt aaggcgcgtc tccattactt ccacttcctg ttatatgatt attgcctatc
2941 aaaatagtgt ctgtacttcc cagcgttctt gatgtgctac agggaacatg gcctgcagaa
3001 aagggtccat tggccaaagg ctgcccaggg caggcaggac ggactccagg ctgagagacg
3061 ctaggcactc tggtcagagc aagctgtggc tgctggccag agactgagtt tgtaggcagt
3121 gatgagtcag ctgttggccc attaggaatt ccagtagatc gtacctgtgc aactcctgtt
3181 ggtctcattt ggtgttgttg cattaaggca aactgacttt ccagctgttc cagcatcagt
3241 ttctgtgctg gatttaaatt atttctattt gcgcggagct gttccaaatg ctgtaatttc
3301 tgtggtgtca aacaccatga atgagcttgt tgctgtactg gaggatgtga cacagcatgt
3361 ccaccactcc aattgtcaga agtattctgc tgtgctgtgt tcatggcacc ctgcctggag
3421 gtaagctctg cgggaattgg taggctccac gcctcctcaa tactaggaag taatttagtt
3481 ttattctgta gactaccttg tggaaggtta cacaactgag cctgtaaata cttaattcgt
3541 gctgcaagtg cagaggtatt actacaactt ttgcttctag ttgcatttaa gtagcattta
3601 atggcatcct gaggctggtt gcaggattca tagagagtgc ctaggtccat ccaggctgca
3661 gcatggccat ggtccaattg tacagcacaa atataggcct gtaaagcatc catgggctga
3721 ttttgctgct gatatagcac acctattgaa caccatgtat ctgcacttgc ttctgattta
3781 tcaatagact gcctgtaaga tataaaggca tcctgaactt tcccaatact tgaatagcac
3841 cttccgagga aataccagga ctggccagaa ttaggatctg cttccaagga cttttggaga
3901 tactgaatag catagctttc cttggtggct ttatctccca ggaggtctac agtgtgatgc
3961 atccaaccta actgttgtaa gacagttgct tttacttgtg cagaaagatt ctctgtctgc
4021 aaaagttgtt cataagcttc ttttgcagaa tgatattccc tctgggtttc atataagtgg
4081 gcaatgtgaa attgaatttc agcattggac aaagtgcagg gattacagtc aaccaaagct
4141 aactgaaaat gctttaaact agactcatag tctgtgttca ctttgaacat aagcccaagt
4201 cgtaaatgaa tttccttggc tcgacaaaag ctgggatcaa cataaagcac ctcctgaaat
4261 gctttaattg cccactgaaa tgcattataa tggaagtaga ccaaaccaag accatataaa
4321 aaggcagcat tcttccagta gtcagactgt aaactgtagt acctctggta tgcagataat
4381 gcttttggat aatcttccaa taagaggttg aagtgaccta attgacaaaa gaaatcagac
4441 tccacttttc cttcagcttt taagattaga gattcatagc agcgaacagc cttgcccagt
4501 agggccttcg tcctggcgcc atcttcatga aatctcacga acccgaagag gcggctgtcc
4561 agtccgccga gcgcctccct ctcctcggct gtcaggctgg gggacgcctc ctcgctctcg
4621 ccgctcgctt ttcccgccgc cattttcttt tcctcatcac cgaaagcggc ggcggcagcg
4681 gcggcggtag cgagcgacac tccgcaggat ttcatctcga gaggccttga attcaagtcc
4741 tcttcagaaa tgagcttttg ctccatggtg aggtcgccca agctctccat ttcattcaag
4801 tcctcttcag aaatgagctt ttgctccatt tcattcaagt cctcttcaga aatgagcttt
4861 tgctccattt cattcaagtc ctcttcagaa atgagctttt gctccatttc attcaagtcc
4921 tcttcagaaa tgagcttttg ctccatttca ttcaagtcct cttcagaaat gagcttttgc
4981 tccatagctt tcgatcgatt taccttacgc ttcttctttg gagccatggc ggcggatcct
5041 gcaaaaagaa caagtagctt gtattctata gtgtcaccta aatcaagctt gctccgacgt
5101 ccccaggcag aatggcggtt ccctaaacga gcattgctta tatagacctc ccattaggca
5161 cgcctaccgc ccatttacgt caatggaacg cccatttgcg tcattgcccc tccccattga
5221 cgtcaatggg gatgtacttg gcagccatcg cgggccattt accgccattg acgtcaatgg
5281 gagtactgcc aatgtaccct ggcgtacttc caatagtaat gtacttgcca agttactatt
5341 aatagatatt gatgtactgc caagtgggcc atttaccgtc attgacgtca atagggggcg
5401 tgagaacgga tatgaatggg caatgagcca tcccattgac gtcaatggtg ggtggtccta
5461 ttgacgtcaa tgggcattga gccaggcggg ccatttaccg taattgacgt caatggggga
5521 ggcgccatat acgtcaatag gaccgcccat atgacgtcaa taggaaagac catatataga
5581 gaccattgac gtcaatgggg gagtggctat gggcggtatt aggaagcccc atatatggta
5641 tatgggaccg cccattggga ggggctatct acgtcaatag gaaaacccat atatggaata
5701 ctatatggca tagggccaat acatagtatt gaacctggcc aatagccata ttggcatagg
5761 gccatattgg atattgccta tatattgatc ctggcatata gccaatatgg ccgccattat
5821 tggcaccatg ccaattcaat atggcggact tggcacaggg ccaagacccc tcctcaaact
5881 tggcaccgtg ccaagtagac ccctccccag ttggcacagt gccaggtccg ccatattgaa
5941 ttggcatggt gccaagtccg ccatattgaa ttggctgggg tccagatcca ccatattgaa
6001 ttggcacgag tccatatacg ccatattgaa ttggcacagg tccagatcca ccatattgaa
6061 ttggcatgag tccatatacg ccatattgaa ttggcggtcg actggcgtaa tagcgaagag
6121 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
6181 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
6241 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
6301 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
6361 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
6421 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
6481 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
6541 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
6601 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
6661 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
6721 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
6781 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
6841 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
6901 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
6961 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
7021 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
7081 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
7141 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
7201 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
7261 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
7321 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
7381 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
7441 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
7501 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
7561 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
7621 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
7681 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
7741 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
7801 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
7861 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
7921 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
7981 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
8041 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
8101 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
8161 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
8221 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
8281 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
8341 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
8401 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
8461 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
8521 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
8581 ctctccccgc gcgttggccg attcattaat g
//
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