pCMV delta R8.2

价格:1200元

联系方式:I47-825O-882O

相关技术服务:质粒构建    基因合成    质粒大提

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载体基本信息

质粒名称: pCMV delta R8.2 
出品公司: 爱迪基因
目录编号: 12263
存储实验室: Didier Trono

载体骨架基本信息
载体名称: pCMVR8.2
载体抗性: 氨苄霉素
空载体大小: 8128 bp
载体类型: 哺乳细胞表达,慢病毒,包装
高拷贝/低拷贝: 高拷贝
生长条件: 37 ℃

插入基因信息
插入基因名称: HIV-1 GAG/POL, Tat and Rev
克隆方法: 限制性内切酶
5' 测序引物 CMV-F

文献引用方法
材料和方法部分: pCMV delta R8.2 was a gift from Didier Trono (Addgene plasmid # 12263)

载体图谱质粒图谱和多克隆位点信息

pCMV delta R8.2载体图谱

载体简介

载体序列

LOCUS       pCMV delta R8.2 13463 bp  DNA SYN
DEFINITION  pCMV delta R8.2
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..13463
                     /organism="pCMV delta R8.2"
                     /mol_type="other DNA"
     promoter        27..603
                     /label="CMV_immearly_promoter"
     misc_feature    106..393
                     /label="CAG_enhancer"
     misc_feature    560..580
                     /label="CMV_fwd_primer"
     promoter        561..630
                     /label="CMV_promoter"
     promoter        573..692
                     /label="CMV2_promoter"
     misc_feature    604..623
                     /label="pCEP_fwd_primer"
     misc_feature    606..630
                     /label="LNCX_primer"
     gene            880..2388
                     /label="Gag_HIV (variant)"
                     /gene="Gag_HIV (variant)"
     CDS             880..2388
                     /label="ORF frame 1"
     CDS             2454..5192
                     /label="ORF frame 3"
     misc_feature    4881..4896
                     /label="cPPT"
     CDS             5137..5715
                     /label="ORF frame 1"
     CDS             complement(6233..6736)
                     /label="ORF frame 2"
     CDS             6441..7589
                     /label="ORF frame 3"
     misc_feature    6563..6796
                     /label="RRE"
     misc_feature    7319..7348
                     /label="HIV_Rev_NES"
     CDS             7591..8211
                     /label="ORF frame 1"
     misc_feature    7863..7878
                     /label="cPPT"
     misc_feature    7863..7884
                     /label="U3PPT"
     misc_feature    complement(8583..8600)
                     /label="Sp6_primer"
     gene            8839..9102
                     /label="tet (300 - 563)"
                     /gene="tet (300 - 563)"
     misc_feature    complement(8891..8910)
                     /label="pBRrevBam_primer"
     misc_feature    9244..9266
                     /label="pGEX_3_primer"
     promoter        9425..9453
                     /label="AmpR_promoter"
     gene            9495..10355
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             9495..10355
                     /label="ORF frame 3"
     rep_origin      10510..11129
                     /label="pBR322_origin"
     misc_feature    complement(11355..11375)
                     /label="pBABE_3_primer"
     misc_feature    complement(11361..11814)
                     /label="SV40_enhancer"
     promoter        11373..11641
                     /label="SV40_promoter"
     rep_origin      11540..11617
                     /label="SV40_origin"
     misc_feature    11602..11621
                     /label="SV40pro_F_primer"
     CDS             11889..12347
                     /label="ORF frame 3"
     misc_feature    12823..12838
                     /label="SV40_int"
     misc_feature    12844..12891
                     /label="SV40_3_splice"
     CDS             12968..13441
                     /label="ORF frame 2"
     misc_feature    13019..13039
                     /label="SV40 NLS"
     misc_feature    13025..13039
                     /label="NLS"
ORIGIN
    1 gggctgcagg aattcgagct cgcccgacat tgattattga ctagttatta atagtaatca
   61 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
  121 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
  181 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
  241 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
  301 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
  361 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
  421 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
  481 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
  541 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
  601 agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac
  661 ctccatagaa gacaccggga ccgatccagc ctccgcggcc gggaacggtg cattggaacg
  721 cggattcccc gtgccaagag tgacgtaagt accgcctata gagtctatag gcccaccccc
  781 ttggcttctt atgcgacgga tcgatcccgt aataagcttc gaggtccgcg gccgcgttga
  841 cgcgcacggc aagaggcgag gggcggcgac tggtgagaga tgggtgcgag agcgtcagta
  901 ttaagcgggg gagaattaga tcgatgggaa aaaattcggt taaggccagg gggaaagaaa
  961 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
 1021 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
 1081 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
 1141 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
 1201 caaaacaaaa gtaagaaaaa agcacagcaa gcagcagctg acacaggaca cagcaatcag
 1261 gtcagccaaa attaccctat agtgcagaac atccaggggc aaatggtaca tcaggccata
 1321 tcacctagaa ctttaaatgc atgggtaaaa gtagtagaag agaaggcttt cagcccagaa
 1381 gtgataccca tgttttcagc attatcagaa ggagccaccc cacaagattt aaacaccatg
 1441 ctaaacacag tggggggaca tcaagcagcc atgcaaatgt taaaagagac catcaatgag
 1501 gaagctgcag aatgggatag agtgcatcca gtgcatgcag ggcctattgc accaggccag
 1561 atgagagaac caaggggaag tgacatagca ggaactacta gtactagtac ccttcaggaa
 1621 caaataggat ggatgacaca taatccacct atcccagtag gagaaatcta taaaagatgg
 1681 ataatcctgg gattaaataa aatagtaaga atgtatagcc ctaccagcat tctggacata
 1741 agacaaggac caaaggaacc ctttagagac tatgtagacc gattctataa aactctaaga
 1801 gccgagcaag cttcacaaga ggtaaaaaat tggatgacag aaaccttgtt ggtccaaaat
 1861 gcgaacccag attgtaagac tattttaaaa gcattgggac caggagcgac actagaagaa
 1921 atgatgacag catgtcaggg agtgggggga cccggccata aagcaagagt tttggctgaa
 1981 gcaatgagcc aagtaacaaa tccagctacc ataatgatac agaaaggcaa ttttaggaac
 2041 caaagaaaga ctgttaagtg tttcaattgt ggcaaagaag ggcacatagc caaaaattgc
 2101 agggccccta ggaaaaaggg ctgttggaaa tgtggaaagg aaggacacca aatgaaagat
 2161 tgtactgaga gacaggctaa ttttttaggg aagatctggc cttcccacaa gggaaggcca
 2221 gggaattttc ttcagagcag accagagcca acagccccac cagaagagag cttcaggttt
 2281 ggggaagaga caacaactcc ctctcagaag caggagccga tagacaagga actgtatcct
 2341 ttagcttccc tcagatcact ctttggcagc gacccctcgt cacaataaag ataggggggc
 2401 aattaaagga agctctatta gatacaggag cagatgatac agtattagaa gaaatgaatt
 2461 tgccaggaag atggaaacca aaaatgatag ggggaattgg aggttttatc aaagtaggac
 2521 agtatgatca gatactcata gaaatctgcg gacataaagc tataggtaca gtattagtag
 2581 gacctacacc tgtcaacata attggaagaa atctgttgac tcagattggc tgcactttaa
 2641 attttcccat tagtcctatt gagactgtac cagtaaaatt aaagccagga atggatggcc
 2701 caaaagttaa acaatggcca ttgacagaag aaaaaataaa agcattagta gaaatttgta
 2761 cagaaatgga aaaggaagga aaaatttcaa aaattgggcc tgaaaatcca tacaatactc
 2821 cagtatttgc cataaagaaa aaagacagta ctaaatggag aaaattagta gatttcagag
 2881 aacttaataa gagaactcaa gatttctggg aagttcaatt aggaatacca catcctgcag
 2941 ggttaaaaca gaaaaaatca gtaacagtac tggatgtggg cgatgcatat ttttcagttc
 3001 ccttagataa agacttcagg aagtatactg catttaccat acctagtata aacaatgaga
 3061 caccagggat tagatatcag tacaatgtgc ttccacaggg atggaaagga tcaccagcaa
 3121 tattccagtg tagcatgaca aaaatcttag agccttttag aaaacaaaat ccagacatag
 3181 tcatctatca atacatggat gatttgtatg taggatctga cttagaaata gggcagcata
 3241 gaacaaaaat agaggaactg agacaacatc tgttgaggtg gggatttacc acaccagaca
 3301 aaaaacatca gaaagaacct ccattccttt ggatgggtta tgaactccat cctgataaat
 3361 ggacagtaca gcctatagtg ctgccagaaa aggacagctg gactgtcaat gacatacaga
 3421 aattagtggg aaaattgaat tgggcaagtc agatttatgc agggattaaa gtaaggcaat
 3481 tatgtaaact tcttagggga accaaagcac taacagaagt agtaccacta acagaagaag
 3541 cagagctaga actggcagaa aacagggaga ttctaaaaga accggtacat ggagtgtatt
 3601 atgacccatc aaaagactta atagcagaaa tacagaagca ggggcaaggc caatggacat
 3661 atcaaattta tcaagagcca tttaaaaatc tgaaaacagg aaaatatgca agaatgaagg
 3721 gtgcccacac taatgatgtg aaacaattaa cagaggcagt acaaaaaata gccacagaaa
 3781 gcatagtaat atggggaaag actcctaaat ttaaattacc catacaaaag gaaacatggg
 3841 aagcatggtg gacagagtat tggcaagcca cctggattcc tgagtgggag tttgtcaata
 3901 cccctccctt agtgaagtta tggtaccagt tagagaaaga acccataata ggagcagaaa
 3961 ctttctatgt agatggggca gccaataggg aaactaaatt aggaaaagca ggatatgtaa
 4021 ctgacagagg aagacaaaaa gttgtccccc taacggacac aacaaatcag aagactgagt
 4081 tacaagcaat tcatctagct ttgcaggatt cgggattaga agtaaacata gtgacagact
 4141 cacaatatgc attgggaatc attcaagcac aaccagataa gagtgaatca gagttagtca
 4201 gtcaaataat agagcagtta ataaaaaagg aaaaagtcta cctggcatgg gtaccagcac
 4261 acaaaggaat tggaggaaat gaacaagtag atgggttggt cagtgctgga atcaggaaag
 4321 tactattttt agatggaata gataaggccc aagaagaaca tgagaaatat cacagtaatt
 4381 ggagagcaat ggctagtgat tttaacctac cacctgtagt agcaaaagaa atagtagcca
 4441 gctgtgataa atgtcagcta aaaggggaag ccatgcatgg acaagtagac tgtagcccag
 4501 gaatatggca gctagattgt acacatttag aaggaaaagt tatcttggta gcagttcatg
 4561 tagccagtgg atatatagaa gcagaagtaa ttccagcaga gacagggcaa gaaacagcat
 4621 acttcctctt aaaattagca ggaagatggc cagtaaaaac agtacataca gacaatggca
 4681 gcaatttcac cagtactaca gttaaggccg cctgttggtg ggcggggatc aagcaggaat
 4741 ttggcattcc ctacaatccc caaagtcaag gagtaataga atctatgaat aaagaattaa
 4801 agaaaattat aggacaggta agagatcagg ctgaacatct taagacagca gtacaaatgg
 4861 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
 4921 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
 4981 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg aaaggaccag
 5041 caaagctcct ctggaaaggt gaaggggcag tagtaataca agataatagt gacataaaag
 5101 tagtgccaag aagaaaagca aagatcatca gggattatgg aaaacagatg gcaggtgatg
 5161 attgtgtggc aagtagacag gatgaggatt aacacatgga aaagattagt aaaacaccat
 5221 atgtatattt caaggaaagc taaggactgg ttttatagac atcactatga aagtactaat
 5281 ccaaaaataa gttcagaagt acacatccca ctaggggatg ctaaattagt aataacaaca
 5341 tattggggtc tgcatacagg agaaagagac tggcatttgg gtcagggagt ctccatagaa
 5401 tggaggaaaa agagatatag cacacaagta gaccctgacc tagcagacca actaattcat
 5461 ctgcactatt ttgattgttt ttcagaatct gctataagaa ataccatatt aggacgtata
 5521 gttagtccta ggtgtgaata tcaagcagga cataacaagg taggatctct acagtacttg
 5581 gcactagcag cattaataaa accaaaacag ataaagccac ctttgcctag tgttaggaaa
 5641 ctgacagagg acagatggaa caagccccag aagaccaagg gccacagagg gagccataca
 5701 atgaatggac actagagctt ttagaggaac ttaagagtga agctgttaga cattttccta
 5761 ggatatggct ccataactta ggacaacata tctatgaaac ttacggggat acttgggcag
 5821 gagtggaagc cataataaga attctgcaac aactgctgtt tatccatttc agaattgggt
 5881 gtcgacatag cagaataggc gttactcgac agaggagagc aagaaatgga gccagtagat
 5941 cctagactag agccctggaa gcatccagga agtcagccta aaactgcttg taccaattgc
 6001 tattgtaaaa agtgttgctt tcattgccaa gtttgtttca tgacaaaagc cttaggcatc
 6061 tcctatggca ggaagaagcg gagacagcga cgaagagctc atcagaacag tcagactcat
 6121 caagcttctc tatcaaagca gtaagtagta catgtaatgc aacctataat agtagcaata
 6181 gtagcattag tagtagcaat aataatagca atagttgtgt ggtccatagt aatcatagaa
 6241 tataggaaaa tattaagaca aagaaaaata gacaggttaa ttgatagact aatagaaaga
 6301 gcagaagaca gtggcaatga gagtgaagga gaagtatcag cacttgtgga gatgggggtg
 6361 gaaatggggc accatgctcc ttgggatatt gatgatctgt agttgagcgg ccgctgatct
 6421 tcagacttgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag
 6481 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga
 6541 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa
 6601 gcactatggg cgcagcctca atgacgctga cggtacaggc cagacaatta ttgtctggta
 6661 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac
 6721 tcacagtctg gggcatcaag cagctccaag caagaatcct agctgtggaa agatacctaa
 6781 aggatcaaca gctcctaggg atttggggtt gctctggaaa actcatttgc accactgctg
 6841 tgccttggaa tgctagttgg agtaataaat ctctggaaca gatctggaat cacacgacct
 6901 ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag
 6961 aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa
 7021 gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga
 7081 tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag
 7141 ttaggcaggg atattcacca ttatcgtttc agacccacct cccaatcccg aggggacccg
 7201 acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat
 7261 tagtgaacgg atccttggca cttatctggg acgatctgcg gagcctgtgc ctcttcagct
 7321 accaccgctt gagagactta ctcttgattg taacgaggat tgtggaactt ctgggacgca
 7381 gggggtggga agccctcaaa tattggtgga atctcctaca atattggagt caggagctaa
 7441 agaatagtgc tgttagcttg ctcaatgcca cagccatagc agtagctgag gggacagata
 7501 gggttataga agtagtacaa ggagcttgta gagctattcg ccacatacct agaagaataa
 7561 gacagggctt ggaaaggatt ttgctataag atgggtggca agtggtcaaa aagtagtgtg
 7621 attggatggc ctactgtaag ggaaagaatg agacgagctg agccagcagc agatggggtg
 7681 ggagcagtat ctcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct
 7741 accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc
 7801 acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
 7861 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatatcctt
 7921 gatctgtgga tctaccacac acaaggctac ttccctgatt ggcagaacta cacaccaggg
 7981 ccagggatca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcaa
 8041 gagaaggtag aagaagccaa tgaaggagag aacacccgct tgttacaccc tgtgagcctg
 8101 catgggatgg atgacccgga gagagaagta ttagagtgga ggtttgacag ccgcctagca
 8161 tttcatcaca tggcccgaga gctgcatccg gagtacttca agaactgctg agcggccgcc
 8221 ccggtgacct tcagaccttg gcactggagg tggcccggca gaagcgcggc atcgtggatc
 8281 agtgctgcac cagcatctgc tctctctacc aactggagaa ctactgcaac taggcccacc
 8341 actaccctgt ccacccctct gcaatgaata aaacctttga aagagcacta caagttgtgt
 8401 gtacatgcgt gcatgtgcat atgtggtgcg gggggaacat gagtggggct ggctggagtg
 8461 gcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct
 8521 atttttatag gttaatgtca tgataataat ggtttcttag tctagaatta attccgtgta
 8581 ttctatagtg tcacctaaat cgtatgtgta tgatacataa ggttatgtat taattgtagc
 8641 cgcgttctaa cgacaatatg tacaagccta attgtgtagc atctggctta ctgaagcaga
 8701 ccctatcatc tctctcgtaa actgccgtca gagtcggttt ggttggacga accttctgag
 8761 tttctggtaa cgccgttccg caccccggaa atggtcagcg aaccaatcag cagggtcatc
 8821 gctagccaga tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg
 8881 gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg
 8941 ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg
 9001 ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta
 9061 ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgata tggtgcactc
 9121 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
 9181 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
 9241 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
 9301 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
 9361 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
 9421 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
 9481 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
 9541 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
 9601 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
 9661 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
 9721 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
 9781 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
 9841 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
 9901 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
 9961 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
10021 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
10081 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
10141 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
10201 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
10261 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
10321 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
10381 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
10441 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
10501 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
10561 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
10621 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
10681 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
10741 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
10801 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
10861 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
10921 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
10981 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
11041 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
11101 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
11161 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
11221 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
11281 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
11341 attaatgcag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
11401 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
11461 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
11521 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
11581 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
11641 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttggaca
11701 caagacaggc ttgcgagata tgtttgagaa taccacttta tcccgcgtca gggagaggca
11761 gtgcgtaaaa agacgcggac tcatgtgaaa tactggtttt tagtgcgcca gatctctata
11821 atctcgcgca acctattttc ccctcgaaca ctttttaagc cgtagataaa caggctggga
11881 cacttcacat gagcgaaaaa tacatcgtca cctgggacat gttgcagatc catgcacgta
11941 aactcgcaag ccgactgatg ccttctgaac aatggaaagg cattattgcc gtaagccgtg
12001 gcggtctggt accgggtgcg ttactggcgc gtgaactggg tattcgtcat gtcgataccg
12061 tttgtatttc cagctacgat cacgacaacc agcgcgagct taaagtgctg aaacgcgcag
12121 aaggcgatgg cgaaggcttc atcgttattg atgacctggt ggataccggt ggtactgcgg
12181 ttgcgattcg tgaaatgtat ccaaaagcgc actttgtcac catcttcgca aaaccggctg
12241 gtcgtccgct ggttgatgac tatgttgttg atatcccgca agatacctgg attgaacagc
12301 cgtgggatat gggcgtcgta ttcgtcccgc caatctccgg tcgctaatct tttcaacgcc
12361 tggcactgcc gggcgttgtt ctttttaact tcaggcgggt tacaatagtt tccagtaagt
12421 attctggagg ctgcatccat gacacaggca aacctgagcg aaaccctgtt caaaccccgc
12481 tttaaacatc ctgaaacctc gacgctagtc cgccgcttta atcacggcgc acaaccgcct
12541 gtgcagtcgg cccttgatgg taaaaccatc cctcactggt atcgcatgat taaccgtctg
12601 atgtggatct ggcgcggcat tgacccacgc gaaatcctcg acgtccaggc acgtattgtg
12661 atgagcgatg ccgaacgtac cgacgatgat ttatacgata cggtgattgg ctaccgtggc
12721 ggcaactgga tttatgagtg ggccccggat ctttgtgaag gaaccttact tctgtggtgt
12781 gacataattg gacaaactac ctacagagat ttaaagctct aaggtaaata taaaattttt
12841 aagtgtataa tgtgttaaac tactgattct aattgtttgt gtattttaga ttccaaccta
12901 tggaactgat gaatgggagc agtggtggaa tgcctttaat gaggaaaacc tgttttgctc
12961 agaagaaatg ccatctagtg atgatgaggc tactgctgac tctcaacatt ctactcctcc
13021 aaaaaagaag agaaaggtag aagaccccaa ggactttcct tcagaattgc taagtttttt
13081 gagtcatgct gtgtttagta atagaactct tgcttgcttt gctatttaca ccacaaagga
13141 aaaagctgca ctgctataca agaaaattat ggaaaaatat tctgtaacct ttataagtag
13201 gcataacagt tataatcata acatactgtt ttttcttact ccacacaggc atagagtgtc
13261 tgctattaat aactatgctc aaaaattgtg tacctttagc tttttaattt gtaaaggggt
13321 taataaggaa tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat
13381 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
13441 aaatgaatgc aattgttgtt gtt
//

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