质粒名称: | pCMV delta R8.2 |
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出品公司: | 爱迪基因 |
目录编号: | 12263 |
存储实验室: | Didier Trono |
载体骨架基本信息 |
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载体名称: | pCMVR8.2 |
载体抗性: | 氨苄霉素 |
空载体大小: | 8128 bp |
载体类型: | 哺乳细胞表达,慢病毒,包装 |
高拷贝/低拷贝: | 高拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | HIV-1 GAG/POL, Tat and Rev |
克隆方法: | 限制性内切酶 |
5' 测序引物 | CMV-F |
文献引用方法 |
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材料和方法部分: | pCMV delta R8.2 was a gift from Didier Trono (Addgene plasmid # 12263) |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCMV delta R8.2 13463 bp DNA SYN DEFINITION pCMV delta R8.2 ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..13463 /organism="pCMV delta R8.2" /mol_type="other DNA" promoter 27..603 /label="CMV_immearly_promoter" misc_feature 106..393 /label="CAG_enhancer" misc_feature 560..580 /label="CMV_fwd_primer" promoter 561..630 /label="CMV_promoter" promoter 573..692 /label="CMV2_promoter" misc_feature 604..623 /label="pCEP_fwd_primer" misc_feature 606..630 /label="LNCX_primer" gene 880..2388 /label="Gag_HIV (variant)" /gene="Gag_HIV (variant)" CDS 880..2388 /label="ORF frame 1" CDS 2454..5192 /label="ORF frame 3" misc_feature 4881..4896 /label="cPPT" CDS 5137..5715 /label="ORF frame 1" CDS complement(6233..6736) /label="ORF frame 2" CDS 6441..7589 /label="ORF frame 3" misc_feature 6563..6796 /label="RRE" misc_feature 7319..7348 /label="HIV_Rev_NES" CDS 7591..8211 /label="ORF frame 1" misc_feature 7863..7878 /label="cPPT" misc_feature 7863..7884 /label="U3PPT" misc_feature complement(8583..8600) /label="Sp6_primer" gene 8839..9102 /label="tet (300 - 563)" /gene="tet (300 - 563)" misc_feature complement(8891..8910) /label="pBRrevBam_primer" misc_feature 9244..9266 /label="pGEX_3_primer" promoter 9425..9453 /label="AmpR_promoter" gene 9495..10355 /label="Ampicillin" /gene="Ampicillin" CDS 9495..10355 /label="ORF frame 3" rep_origin 10510..11129 /label="pBR322_origin" misc_feature complement(11355..11375) /label="pBABE_3_primer" misc_feature complement(11361..11814) /label="SV40_enhancer" promoter 11373..11641 /label="SV40_promoter" rep_origin 11540..11617 /label="SV40_origin" misc_feature 11602..11621 /label="SV40pro_F_primer" CDS 11889..12347 /label="ORF frame 3" misc_feature 12823..12838 /label="SV40_int" misc_feature 12844..12891 /label="SV40_3_splice" CDS 12968..13441 /label="ORF frame 2" misc_feature 13019..13039 /label="SV40 NLS" misc_feature 13025..13039 /label="NLS" ORIGIN 1 gggctgcagg aattcgagct cgcccgacat tgattattga ctagttatta atagtaatca 61 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta 121 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat 181 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg 241 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac 301 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt 361 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg 421 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc 481 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt 541 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata 601 agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac 661 ctccatagaa gacaccggga ccgatccagc ctccgcggcc gggaacggtg cattggaacg 721 cggattcccc gtgccaagag tgacgtaagt accgcctata gagtctatag gcccaccccc 781 ttggcttctt atgcgacgga tcgatcccgt aataagcttc gaggtccgcg gccgcgttga 841 cgcgcacggc aagaggcgag gggcggcgac tggtgagaga tgggtgcgag agcgtcagta 901 ttaagcgggg gagaattaga tcgatgggaa aaaattcggt taaggccagg gggaaagaaa 961 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat 1021 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc 1081 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt 1141 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag 1201 caaaacaaaa gtaagaaaaa agcacagcaa gcagcagctg acacaggaca cagcaatcag 1261 gtcagccaaa attaccctat agtgcagaac atccaggggc aaatggtaca tcaggccata 1321 tcacctagaa ctttaaatgc atgggtaaaa gtagtagaag agaaggcttt cagcccagaa 1381 gtgataccca tgttttcagc attatcagaa ggagccaccc cacaagattt aaacaccatg 1441 ctaaacacag tggggggaca tcaagcagcc atgcaaatgt taaaagagac catcaatgag 1501 gaagctgcag aatgggatag agtgcatcca gtgcatgcag ggcctattgc accaggccag 1561 atgagagaac caaggggaag tgacatagca ggaactacta gtactagtac ccttcaggaa 1621 caaataggat ggatgacaca taatccacct atcccagtag gagaaatcta taaaagatgg 1681 ataatcctgg gattaaataa aatagtaaga atgtatagcc ctaccagcat tctggacata 1741 agacaaggac caaaggaacc ctttagagac tatgtagacc gattctataa aactctaaga 1801 gccgagcaag cttcacaaga ggtaaaaaat tggatgacag aaaccttgtt ggtccaaaat 1861 gcgaacccag attgtaagac tattttaaaa gcattgggac caggagcgac actagaagaa 1921 atgatgacag catgtcaggg agtgggggga cccggccata aagcaagagt tttggctgaa 1981 gcaatgagcc aagtaacaaa tccagctacc ataatgatac agaaaggcaa ttttaggaac 2041 caaagaaaga ctgttaagtg tttcaattgt ggcaaagaag ggcacatagc caaaaattgc 2101 agggccccta ggaaaaaggg ctgttggaaa tgtggaaagg aaggacacca aatgaaagat 2161 tgtactgaga gacaggctaa ttttttaggg aagatctggc cttcccacaa gggaaggcca 2221 gggaattttc ttcagagcag accagagcca acagccccac cagaagagag cttcaggttt 2281 ggggaagaga caacaactcc ctctcagaag caggagccga tagacaagga actgtatcct 2341 ttagcttccc tcagatcact ctttggcagc gacccctcgt cacaataaag ataggggggc 2401 aattaaagga agctctatta gatacaggag cagatgatac agtattagaa gaaatgaatt 2461 tgccaggaag atggaaacca aaaatgatag ggggaattgg aggttttatc aaagtaggac 2521 agtatgatca gatactcata gaaatctgcg gacataaagc tataggtaca gtattagtag 2581 gacctacacc tgtcaacata attggaagaa atctgttgac tcagattggc tgcactttaa 2641 attttcccat tagtcctatt gagactgtac cagtaaaatt aaagccagga atggatggcc 2701 caaaagttaa acaatggcca ttgacagaag aaaaaataaa agcattagta gaaatttgta 2761 cagaaatgga aaaggaagga aaaatttcaa aaattgggcc tgaaaatcca tacaatactc 2821 cagtatttgc cataaagaaa aaagacagta ctaaatggag aaaattagta gatttcagag 2881 aacttaataa gagaactcaa gatttctggg aagttcaatt aggaatacca catcctgcag 2941 ggttaaaaca gaaaaaatca gtaacagtac tggatgtggg cgatgcatat ttttcagttc 3001 ccttagataa agacttcagg aagtatactg catttaccat acctagtata aacaatgaga 3061 caccagggat tagatatcag tacaatgtgc ttccacaggg atggaaagga tcaccagcaa 3121 tattccagtg tagcatgaca aaaatcttag agccttttag aaaacaaaat ccagacatag 3181 tcatctatca atacatggat gatttgtatg taggatctga cttagaaata gggcagcata 3241 gaacaaaaat agaggaactg agacaacatc tgttgaggtg gggatttacc acaccagaca 3301 aaaaacatca gaaagaacct ccattccttt ggatgggtta tgaactccat cctgataaat 3361 ggacagtaca gcctatagtg ctgccagaaa aggacagctg gactgtcaat gacatacaga 3421 aattagtggg aaaattgaat tgggcaagtc agatttatgc agggattaaa gtaaggcaat 3481 tatgtaaact tcttagggga accaaagcac taacagaagt agtaccacta acagaagaag 3541 cagagctaga actggcagaa aacagggaga ttctaaaaga accggtacat ggagtgtatt 3601 atgacccatc aaaagactta atagcagaaa tacagaagca ggggcaaggc caatggacat 3661 atcaaattta tcaagagcca tttaaaaatc tgaaaacagg aaaatatgca agaatgaagg 3721 gtgcccacac taatgatgtg aaacaattaa cagaggcagt acaaaaaata gccacagaaa 3781 gcatagtaat atggggaaag actcctaaat ttaaattacc catacaaaag gaaacatggg 3841 aagcatggtg gacagagtat tggcaagcca cctggattcc tgagtgggag tttgtcaata 3901 cccctccctt agtgaagtta tggtaccagt tagagaaaga acccataata ggagcagaaa 3961 ctttctatgt agatggggca gccaataggg aaactaaatt aggaaaagca ggatatgtaa 4021 ctgacagagg aagacaaaaa gttgtccccc taacggacac aacaaatcag aagactgagt 4081 tacaagcaat tcatctagct ttgcaggatt cgggattaga agtaaacata gtgacagact 4141 cacaatatgc attgggaatc attcaagcac aaccagataa gagtgaatca gagttagtca 4201 gtcaaataat agagcagtta ataaaaaagg aaaaagtcta cctggcatgg gtaccagcac 4261 acaaaggaat tggaggaaat gaacaagtag atgggttggt cagtgctgga atcaggaaag 4321 tactattttt agatggaata gataaggccc aagaagaaca tgagaaatat cacagtaatt 4381 ggagagcaat ggctagtgat tttaacctac cacctgtagt agcaaaagaa atagtagcca 4441 gctgtgataa atgtcagcta aaaggggaag ccatgcatgg acaagtagac tgtagcccag 4501 gaatatggca gctagattgt acacatttag aaggaaaagt tatcttggta gcagttcatg 4561 tagccagtgg atatatagaa gcagaagtaa ttccagcaga gacagggcaa gaaacagcat 4621 acttcctctt aaaattagca ggaagatggc cagtaaaaac agtacataca gacaatggca 4681 gcaatttcac cagtactaca gttaaggccg cctgttggtg ggcggggatc aagcaggaat 4741 ttggcattcc ctacaatccc caaagtcaag gagtaataga atctatgaat aaagaattaa 4801 agaaaattat aggacaggta agagatcagg ctgaacatct taagacagca gtacaaatgg 4861 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa 4921 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa 4981 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg aaaggaccag 5041 caaagctcct ctggaaaggt gaaggggcag tagtaataca agataatagt gacataaaag 5101 tagtgccaag aagaaaagca aagatcatca gggattatgg aaaacagatg gcaggtgatg 5161 attgtgtggc aagtagacag gatgaggatt aacacatgga aaagattagt aaaacaccat 5221 atgtatattt caaggaaagc taaggactgg ttttatagac atcactatga aagtactaat 5281 ccaaaaataa gttcagaagt acacatccca ctaggggatg ctaaattagt aataacaaca 5341 tattggggtc tgcatacagg agaaagagac tggcatttgg gtcagggagt ctccatagaa 5401 tggaggaaaa agagatatag cacacaagta gaccctgacc tagcagacca actaattcat 5461 ctgcactatt ttgattgttt ttcagaatct gctataagaa ataccatatt aggacgtata 5521 gttagtccta ggtgtgaata tcaagcagga cataacaagg taggatctct acagtacttg 5581 gcactagcag cattaataaa accaaaacag ataaagccac ctttgcctag tgttaggaaa 5641 ctgacagagg acagatggaa caagccccag aagaccaagg gccacagagg gagccataca 5701 atgaatggac actagagctt ttagaggaac ttaagagtga agctgttaga cattttccta 5761 ggatatggct ccataactta ggacaacata tctatgaaac ttacggggat acttgggcag 5821 gagtggaagc cataataaga attctgcaac aactgctgtt tatccatttc agaattgggt 5881 gtcgacatag cagaataggc gttactcgac agaggagagc aagaaatgga gccagtagat 5941 cctagactag agccctggaa gcatccagga agtcagccta aaactgcttg taccaattgc 6001 tattgtaaaa agtgttgctt tcattgccaa gtttgtttca tgacaaaagc cttaggcatc 6061 tcctatggca ggaagaagcg gagacagcga cgaagagctc atcagaacag tcagactcat 6121 caagcttctc tatcaaagca gtaagtagta catgtaatgc aacctataat agtagcaata 6181 gtagcattag tagtagcaat aataatagca atagttgtgt ggtccatagt aatcatagaa 6241 tataggaaaa tattaagaca aagaaaaata gacaggttaa ttgatagact aatagaaaga 6301 gcagaagaca gtggcaatga gagtgaagga gaagtatcag cacttgtgga gatgggggtg 6361 gaaatggggc accatgctcc ttgggatatt gatgatctgt agttgagcgg ccgctgatct 6421 tcagacttgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag 6481 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga 6541 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa 6601 gcactatggg cgcagcctca atgacgctga cggtacaggc cagacaatta ttgtctggta 6661 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac 6721 tcacagtctg gggcatcaag cagctccaag caagaatcct agctgtggaa agatacctaa 6781 aggatcaaca gctcctaggg atttggggtt gctctggaaa actcatttgc accactgctg 6841 tgccttggaa tgctagttgg agtaataaat ctctggaaca gatctggaat cacacgacct 6901 ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag 6961 aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa 7021 gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga 7081 tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag 7141 ttaggcaggg atattcacca ttatcgtttc agacccacct cccaatcccg aggggacccg 7201 acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat 7261 tagtgaacgg atccttggca cttatctggg acgatctgcg gagcctgtgc ctcttcagct 7321 accaccgctt gagagactta ctcttgattg taacgaggat tgtggaactt ctgggacgca 7381 gggggtggga agccctcaaa tattggtgga atctcctaca atattggagt caggagctaa 7441 agaatagtgc tgttagcttg ctcaatgcca cagccatagc agtagctgag gggacagata 7501 gggttataga agtagtacaa ggagcttgta gagctattcg ccacatacct agaagaataa 7561 gacagggctt ggaaaggatt ttgctataag atgggtggca agtggtcaaa aagtagtgtg 7621 attggatggc ctactgtaag ggaaagaatg agacgagctg agccagcagc agatggggtg 7681 ggagcagtat ctcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct 7741 accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc 7801 acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt 7861 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatatcctt 7921 gatctgtgga tctaccacac acaaggctac ttccctgatt ggcagaacta cacaccaggg 7981 ccagggatca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcaa 8041 gagaaggtag aagaagccaa tgaaggagag aacacccgct tgttacaccc tgtgagcctg 8101 catgggatgg atgacccgga gagagaagta ttagagtgga ggtttgacag ccgcctagca 8161 tttcatcaca tggcccgaga gctgcatccg gagtacttca agaactgctg agcggccgcc 8221 ccggtgacct tcagaccttg gcactggagg tggcccggca gaagcgcggc atcgtggatc 8281 agtgctgcac cagcatctgc tctctctacc aactggagaa ctactgcaac taggcccacc 8341 actaccctgt ccacccctct gcaatgaata aaacctttga aagagcacta caagttgtgt 8401 gtacatgcgt gcatgtgcat atgtggtgcg gggggaacat gagtggggct ggctggagtg 8461 gcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct 8521 atttttatag gttaatgtca tgataataat ggtttcttag tctagaatta attccgtgta 8581 ttctatagtg tcacctaaat cgtatgtgta tgatacataa ggttatgtat taattgtagc 8641 cgcgttctaa cgacaatatg tacaagccta attgtgtagc atctggctta ctgaagcaga 8701 ccctatcatc tctctcgtaa actgccgtca gagtcggttt ggttggacga accttctgag 8761 tttctggtaa cgccgttccg caccccggaa atggtcagcg aaccaatcag cagggtcatc 8821 gctagccaga tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg 8881 gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg 8941 ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg 9001 ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta 9061 ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgata tggtgcactc 9121 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg 9181 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg 9241 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa 9301 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga 9361 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa 9421 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt 9481 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg 9541 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag 9601 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg 9661 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg 9721 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt 9781 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga 9841 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac 9901 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc 9961 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc 10021 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac 10081 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag 10141 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg 10201 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta 10261 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg 10321 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata 10381 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt 10441 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc 10501 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct 10561 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa 10621 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag 10681 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc 10741 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg 10801 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca 10861 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat 10921 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg 10981 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc 11041 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc 11101 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc 11161 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg 11221 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga 11281 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc 11341 attaatgcag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc 11401 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc 11461 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt 11521 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc 11581 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct 11641 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttggaca 11701 caagacaggc ttgcgagata tgtttgagaa taccacttta tcccgcgtca gggagaggca 11761 gtgcgtaaaa agacgcggac tcatgtgaaa tactggtttt tagtgcgcca gatctctata 11821 atctcgcgca acctattttc ccctcgaaca ctttttaagc cgtagataaa caggctggga 11881 cacttcacat gagcgaaaaa tacatcgtca cctgggacat gttgcagatc catgcacgta 11941 aactcgcaag ccgactgatg ccttctgaac aatggaaagg cattattgcc gtaagccgtg 12001 gcggtctggt accgggtgcg ttactggcgc gtgaactggg tattcgtcat gtcgataccg 12061 tttgtatttc cagctacgat cacgacaacc agcgcgagct taaagtgctg aaacgcgcag 12121 aaggcgatgg cgaaggcttc atcgttattg atgacctggt ggataccggt ggtactgcgg 12181 ttgcgattcg tgaaatgtat ccaaaagcgc actttgtcac catcttcgca aaaccggctg 12241 gtcgtccgct ggttgatgac tatgttgttg atatcccgca agatacctgg attgaacagc 12301 cgtgggatat gggcgtcgta ttcgtcccgc caatctccgg tcgctaatct tttcaacgcc 12361 tggcactgcc gggcgttgtt ctttttaact tcaggcgggt tacaatagtt tccagtaagt 12421 attctggagg ctgcatccat gacacaggca aacctgagcg aaaccctgtt caaaccccgc 12481 tttaaacatc ctgaaacctc gacgctagtc cgccgcttta atcacggcgc acaaccgcct 12541 gtgcagtcgg cccttgatgg taaaaccatc cctcactggt atcgcatgat taaccgtctg 12601 atgtggatct ggcgcggcat tgacccacgc gaaatcctcg acgtccaggc acgtattgtg 12661 atgagcgatg ccgaacgtac cgacgatgat ttatacgata cggtgattgg ctaccgtggc 12721 ggcaactgga tttatgagtg ggccccggat ctttgtgaag gaaccttact tctgtggtgt 12781 gacataattg gacaaactac ctacagagat ttaaagctct aaggtaaata taaaattttt 12841 aagtgtataa tgtgttaaac tactgattct aattgtttgt gtattttaga ttccaaccta 12901 tggaactgat gaatgggagc agtggtggaa tgcctttaat gaggaaaacc tgttttgctc 12961 agaagaaatg ccatctagtg atgatgaggc tactgctgac tctcaacatt ctactcctcc 13021 aaaaaagaag agaaaggtag aagaccccaa ggactttcct tcagaattgc taagtttttt 13081 gagtcatgct gtgtttagta atagaactct tgcttgcttt gctatttaca ccacaaagga 13141 aaaagctgca ctgctataca agaaaattat ggaaaaatat tctgtaacct ttataagtag 13201 gcataacagt tataatcata acatactgtt ttttcttact ccacacaggc atagagtgtc 13261 tgctattaat aactatgctc aaaaattgtg tacctttagc tttttaattt gtaaaggggt 13321 taataaggaa tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat 13381 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata 13441 aaatgaatgc aattgttgtt gtt //
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