| 质粒名称: | pGBW-m4092686 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 153895 |
| 存储实验室: | Ginkgo Bioworks, Benjie Chen |
|
载体骨架基本信息 |
|
| 载体名称: | pG9m-2 backbone |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 1968 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 细菌表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 低拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR |
| 物种来源: | Severe acute respiratory syndrome coronavirus 2 |
| 插入大小: | 8319 bp |
| 基因突变: | Nearly native encoding;inactivating mutations in all ORFs and tIIs restriction sites removed with 5 base polyA tail |
| GenBank编号 | -- |
| 启动子 | Promoter | t7 consensus from NEB, G is +1 |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pGBW-m4092686 was a gift from Ginkgo Bioworks & Benjie Chen (Addgene plasmid # 153895 ; http://n2t.net/addgene:153895 ; RRID:Addgene_153895) |
买家导航
pGBWm4092686载体基本信息
pGBWm4092686载体图谱质粒图谱和多克隆位点信息
pGBWm4092686载体简介
pGBWm4092686载体序列
LOCUS pGBW-m4092686 10355 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Bacterial expression plasmid for fragment of SARS-CoV-2 orf1ab 3pUTR
to viral genome 3pUTR.
ACCESSION .
VERSION .
KEYWORDS pGBW-m4092686
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10355)
TITLE SARS-CoV-2 protein E. coli expression constructs, tagged, for
protein expression and/or purification
REFERENCE 2 (bases 1 to 10355)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10355
/organism="synthetic DNA construct"
/mol_type="other DNA"
terminator 7858..7905
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
terminator 7948..7979
/label=tonB terminator
/note="bidirectional E. coli tonB-P14 transcription
terminator"
promoter 7980..8082
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
CDS 8083..8943
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(8301..8320)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
CDS 8944..9135
/codon_start=1
/gene="rop"
/product="Rop protein, which maintains plasmids at low copy
number"
/label=rop
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
terminator 9159..9186
/label=T7Te terminator
/note="phage T7 early transcription terminator"
rep_origin complement(9198..9785)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
terminator 9807..9836
/label=T3Te terminator
/note="phage T3 early transcription terminator"
promoter 9875..9892
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
1 atggaaagtg agttcagagt ttattctagt gcgaataatt gcacttttga atatgtctct
61 cagccttttc ttatggacct tgaaggaaaa cagggtaatt tcaaaaatct tagggaattt
121 gtgtttaaga atattgatgg ttattttaaa atatattcta agcacacgcc tattaattta
181 gtgcgtgatc tccctcaggg tttttcggct ttagaaccat tggtagattt gccaataggt
241 attaacatca ctaggtttca aactttactt gctttacata gaagttattt gactcctggt
301 gattcttctt caggttggac agctggtgct gcagcttatt atgtgggtta tcttcaacct
361 aggacttttc tattaaaata taatgaaaat ggaaccatta cagatgctgt agactgtgca
421 cttgaccctc tctcagaaac aaagtgtacg ttgaaatcct tcactgtaga aaaaggaatc
481 tatcaaactt ctaactttag agtccaacca acagaatcta ttgttagatt tcctaatatt
541 acaaacttgt gcccttttgg tgaagttttt aacgccacca gatttgcatc tgtttatgct
601 tggaacagga agagaatcag caactgtgtt gctgattatt ctgtcctata taattccgca
661 tcattttcca cttttaagtg ttatggagtg tctcctacta aattaaatga tctctgcttt
721 actaatgtct atgcagattc atttgtaatt agaggtgatg aagtcagaca aatcgctcca
781 gggcaaactg gaaagattgc tgattataat tataaattac cagatgattt tacaggctgc
841 gttatagctt ggaattctaa caatcttgat tctaaggttg gtggtaatta taattacctg
901 tatagattgt ttaggaagtc taatctcaaa ccttttgaga gagatatttc aactgaaatc
961 tatcaggccg gtagcacacc ttgtaatggt gttgaaggtt ttaattgtta ctttccttta
1021 caatcatatg gtttccaacc cactaatggt gttggttacc aaccatacag agtagtagta
1081 ctttcttttg aacttctaca tgcaccagca actgtttgtg gacctaaaaa gtctactaat
1141 ttggttaaaa acaaatgtgt caatttcaac ttcaatggtt taacaggcac aggtgttctt
1201 actgagtcta acaaaaagtt tctgcctttc caacaatttg gcagagacat tgctgacact
1261 actgatgctg tccgtgatcc acagacactt gagattcttg acattacacc atgttctttt
1321 ggtggtgtca gtgttataac accaggaaca aatacttcta accaggttgc tgttctttat
1381 caggatgtta actgcacaga agtccctgtt gctattcatg cagatcaact tactcctact
1441 tggcgtgttt attctacagg ttctaatgtt tttcaaacac gtgcaggctg tttaataggg
1501 gctgaacatg tcaacaactc atatgagtgt gacataccca ttggtgcagg tatatgcgct
1561 agttatcaga ctcagactaa ttctcctcgg cgggcacgta gtgtagctag tcaatccatc
1621 attgcctaca ctatgtcact tggtgcagaa aattcagttg cttactctaa taactctatt
1681 gccataccca caaattttac tattagtgtt accacagaaa ttctaccagt gtctatgacc
1741 aagacatcag tagattgtac aatgtacatt tgtggtgatt caactgaatg cagcaatctt
1801 ttgttgcaat atggcagttt ttgtacacaa ttaaaccgtg ctttaactgg aatagctgtt
1861 gaacaagaca aaaacaccca agaagttttt gcacaagtca aacaaattta caaaacacca
1921 ccaattaaag attttggtgg ttttaatttt tcacaaatat taccagatcc atcaaaacca
1981 agcaagaggt catttattga agatctactt ttcaacaaag tgacacttgc agatgctggc
2041 ttcatcaaac aatatggtga ttgccttggt gatattgctg ctagggacct catttgtgca
2101 caaaagttta acggccttac tgttttgcca cctttgctca cagatgaaat gattgctcaa
2161 tacacttctg cactgttagc gggtacaatc acttctggtt ggacctttgg tgcaggagct
2221 gcattacaaa taccatttgc tatgcaaatg gcttataggt ttaatggtat tggagttaca
2281 cagaatgttc tctatgagaa ccaaaaattg attgccaacc aatttaatag tgctattggc
2341 aaaattcaag actcactttc ttccacagca agtgcacttg gaaaacttca agatgtggtc
2401 aaccaaaatg cacaagcttt aaacacgctt gttaaacaac ttagctccaa ttttggtgca
2461 atttcaagtg ttttaaatga tatcctttca cgtcttgaca aagttgaggc tgaagtgcaa
2521 attgataggt tgatcacagg cagacttcaa agtttgcaga catatgtgac tcaacaatta
2581 attagagctg cagaaatcag agcttctgct aatcttgctg ctactaaaat gtcagagtgt
2641 gtacttggac aatcaaaaag agttgatttt tgtggaaagg gctatcatct tatgtccttc
2701 cctcagtcag cacctcatgg tgtagtcttc ttgcatgtga cttatgtccc tgcacaagaa
2761 aagaacttca caactgctcc tgccatttgt catgatggaa aagcacactt tcctcgtgaa
2821 ggtgtctttg tttcaaatgg cacacactgg tttgtaacac aaaggaattt ttatgaacca
2881 caaatcatta ctacagacaa cacatttgtg tctggtaact gtgatgttgt aataggaatt
2941 gtcaacaaca cagtttatga tcctttgcaa cctgaattag actcattcaa ggaggagtta
3001 gataaatatt ttaagaatca tacatcacca gatgttgatt taggtgacat ctctggcatt
3061 aatgcttcag ttgtaaacat tcaaaaagaa attgaccgcc tcaatgaggt tgccaagaat
3121 ttaaatgaat ctctcatcga tctccaagaa cttggaaagt atgagcagta tataaaatgg
3181 ccatggtaca tttggctagg ttttatagct ggcttgattg ccatagtaat ggtgacaatt
3241 atgctttgct gtatgaccag ttgctgtagt tgtctcaagg gctgttgttc ttgtggatcc
3301 tgctgcaaat ttgatgaaga cgactctgag ccagtgctca aaggagtcaa attacattac
3361 acataaacga acttatggat ttgtttatga gaatcttcac aattggaact tagactttga
3421 agcaaggtga aatcaaggat gctactcctt cagattttgt tcgcgctact gcaacgatac
3481 cgatacaagc ctcactccct ttcggatggc ttattgttgg cgttgcactt cttgctgttt
3541 ttcagagcgc ttccaaaatc ataaccctca aaaagagatg gcaactagca ctctccaagg
3601 gtgttcactt tgtttgcaac ttgctgttgt tgtttgtaac agtttactca caccttttgc
3661 tcgttgctgc tggccttgaa gccccttttc tctatcttta tgctttagtc tacttcttgc
3721 agagtataaa ctttgtaaga ataataatga ggctttggct ttgctggaaa tgccgttcca
3781 aaaacccatt actttatgat gccaactatt ttctttgctg gcatactaat tgttacgact
3841 attgtatacc ttacaatagt gtaacttctt caattgtcat tacttcaggt gatggcacaa
3901 caagtcctat ttctgaacat gactaccaga ttggtggtta tactgaaaaa tgggaatctg
3961 gagtaaaaga ctgtgttgta ttacacagtt acttcacttc agactattac cagctgtact
4021 caactcaatt gagtacagac actggtgttg aacatgttac cttcttcatc tacaataaaa
4081 ttgttgatga gcctgaagaa catgtccaaa ttcacacaat cgacggttca tccggagttg
4141 ttaatccagt aatggaacca atttatgatg aaccgacgac gactactagc gtgcctttgt
4201 aagcacaagc tgatgagtac gaacttatgt actcattcgt ttcggaagag acaggtacgt
4261 taatagttta aagcgtactt ctttttcttg ctttcgtggt attcttgcta gttacactag
4321 ccatccttac tgcgcttcga ttgtgtgcgt actgctgcaa tattgttaac gtgagtcttg
4381 taaaaccttc tttttacgtt tactctcgtg ttaaaaatct gaattcttct agagttcctg
4441 atcttctggt ctaaacgaac taaatattat attagttttt ctgtttggaa ctttaatttt
4501 agccatggca gattccaacg gtactattac cgttgaagag cttaaaaagc tccttgaaca
4561 atggaaccta tagataggtt tcctattcct tacatggatt tgtcttctac aatttgccta
4621 tgccaacagg aataggtttt tgtatataat taagttaatt ttcctctggc tgttatggcc
4681 agtaacttta gcttgttttg tgcttgctgc tgtttacaga ataaattgga tcaccggtgg
4741 aattgctatc gcaatggctt gtcttgtagg cttgatgtgg ctcagctact tcattgcttc
4801 tttcagactg tttgcgcgta cgcgttccat gtggtcattc aatccagaaa ctaacattct
4861 tctcaacgtg ccactccatg gcactattct gaccagaccg cttctagaaa gtgaactcgt
4921 aatcggagct gtgatccttc gtggacatct tcgtattgct ggacaccatc taggacgctg
4981 tgacatcaag gacctgccta aagaaatcac tgttgctaca tcacgaacgc tttcttatta
5041 caaattggga gcttcgcagc gtgtagcagg agactcaggt tttgctgcat acagtcgcta
5101 caggattggc aactataaat taaacacaga ccattccagt agcagtgaca atattgcttt
5161 gcttgtacag taagtgacaa cagatgtttc atctcgttga ctttcaggtt actatagcag
5221 agatattact aattatttag aggactttta aagtttccat ttggaatctt gattacatca
5281 taaacctcat aattaaaaat ttatctaagt cactaactga gaataaatat tctcaattag
5341 atgaagagca accaatggag attgattaaa cgaacatgaa aattattctt ttcttggcac
5401 tgataacact cgctacttgt gagctttatc actaccaaga gtgtgttaga ggtacaacat
5461 agcttttaaa agaaccttgc tcttctggaa catacgaggg caattcacca tttcatcctc
5521 tagctgataa caaatttgca ctgacttgct ttagcactca atttgctttt gcttgtcctg
5581 acggcgtaaa acacgtctat cagttacgtg ccagatcagt ttcacctaaa ctgttcatca
5641 gacaagagga agttcaagaa ctttactctc caatttttct tattgttgcg gcaatagtgt
5701 ttataacact ttgcttcaca ctcaaaagaa agacagaatg attgaacttt cattaattga
5761 cttctatttg tgctttttag cctttctgct attccttgtt ttaatttagc ttattatctt
5821 ttggttctca cttgaactgc aagatcataa tgaaacttgt cacgcctaaa cgaacatgaa
5881 atttcttgtt ttcttaggaa tcatcacaac ttaggctgca tttcaccaag aatgtagttt
5941 acagtcatgt actcaacatc aaccatatgt agttgatgac ccgtgtccta ttcacttcta
6001 ttctaaatgg tatattagag taggagctag aaaatcagca cctttaattg aattgtgcgt
6061 ggatgaggct ggttctaaat cacccattca gtacatcgat atcggtaatt atacagtttc
6121 ctgtttacct tttacaatta attgccagga acctaaattg ggtagtcttg tagtgcgttg
6181 ttcgttctat gaagactttt tagagtatca tgacgttcgt gttgttttag atttcatcta
6241 aacgaacaaa ctaaaatgtc tgataatgga ccccaataac agcgaaatgc accccgcatt
6301 acgtttggtg gaccctcaga ttcaactggc agtaaccaga atggagaacg cagtggggcg
6361 cgatcaaaac aacgtcggcc ccaaggttta cccaataata ctgcgtcttg gttcaccgct
6421 ctcactcaac atggcaagga agaccttaaa ttccctcgag gacaaggcgt tccaattaac
6481 accaatagca gtccagatga ccaaattggc tactaccgaa gagctaccag acgaattcgt
6541 ggtggtgacg gtaaaatgaa agatctcagt ccaagatggt atttctacta cctaggaact
6601 gggccagaag ctggacttcc ctatggtgct aacaaagacg gcatcatatg ggttgcaact
6661 gagggagcct tgaatacacc aaaagatcac attggcaccc gcaatcctgc taacaatgct
6721 gcaatcgtgc tacaacttcc tcaaggaaca acattgccaa aaggcttcta cgcagaaggg
6781 agcagaggcg gcagtcaagc ctcttctcgt tcctcatcac gtagtcgcaa cagttcaaga
6841 aattcaactc caggcagcag taggggaact tctcctgcta gaatggctgg caatggcggt
6901 gatgctgctc ttgctttgct gctgcttgac agattgaacc agcttgagag caaaatgtct
6961 ggtaaaggcc aacaacaaca aggccaaact gtcactaaga aatctgctgc tgaggcttct
7021 aagaagcctc ggcaaaaacg tactgccact aaagcataca atgtaacaca agctttcggc
7081 agacgtggtc cagaacaaac ccaaggaaat tttggggacc aggaactaat cagacaagga
7141 actgattaca aacattggcc gcaaattgca caatttgccc ccagcgcttc agcgttcttc
7201 ggaatgtcgc gcattggcat ggaagtcaca ccttcgggaa cgtggttgac ctacacaggt
7261 gccatcaaat tggatgacaa agatccaaat ttcaaagatc aagtcatttt gctgaataag
7321 catattgacg catacaaaac attcccacca acagagccta aaaaggacaa aaagaagaag
7381 gctgatgaaa ctcaagcctt accgcagaga cagaagaaac agcaaactgt gactcttctt
7441 cctgctgcag atttggatga tttctccaaa caattgcaac aatccatgag cagtgctgac
7501 tcaactcagg cctaaactca tgcagaccac acaaggcaga tgggctatta aaacgttttc
7561 gcttttccgt ttacgatata tagtctactc ttgtgcagaa tgaattctcg taactacata
7621 gcacaagtag atgtagttaa ctttaatctc acatagcaat ctttaatcag tgtgtaacat
7681 tagggaggac ttgaaagagc caccacattt tcaccgaggc cacgcggagt acgatcgagt
7741 gtacagtgaa caatgctagg gagagctgcc tatatggaag agccctaatg tgtaaaatta
7801 attttagtag tgctatcccc atgtgatttt aatagcttct taggagaatg acaaaaacta
7861 gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgcatca ctgaccattt
7921 aaatcatacc tgacctccat agcagaaagt caaaagcctc cgaccggagg cttttgactt
7981 gatcggcacg taagaggttc caactttcac cataatgaaa taagatcact accgggcgta
8041 ttttttgagt tatcgagatt ttcaggagct aaggaagcta aaatgagtat tcaacatttc
8101 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
8161 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
8221 ctggatctca acagcggtaa gatccttgag agtttacgcc ccgaagaacg ttttccaatg
8281 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
8341 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
8401 acagaaaagc atctcacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
8461 atgagtgata acactgcggc caacttactt ctggcaacga tcggaggacc gaaggagcta
8521 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
8581 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
8641 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
8701 gactggatgg aggcggataa agttgcagga tcacttctgc gctcggccct cccggctggc
8761 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
8821 ctggggccag atggtaagcc ctcccgcatc gtagttatct acacgacggg gagtcaggca
8881 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
8941 taagtgacca aacaggaaaa aaccgccctt aacatggccc gctttatcag aagccagaca
9001 ttaacgcttc tggagaaact caacgagctg gacgcggatg aacaggcaga catctgtgaa
9061 tcgcttcacg accacgctga tgagctttac cgcagctgcc tcgcgcgttt cggtgatgac
9121 ggtgaaaacc tctgatgagg gcccaaatgt aatcacctgg ctcaccttcg ggtgggcctt
9181 tctgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
9241 tgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttacccct
9301 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
9361 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
9421 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
9481 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
9541 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
9601 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
9661 ctgctgaagc cagttacctc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
9721 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
9781 ctcaagaaga tcctttgatt ttctaccgaa gaaaggccca cccgtgaagg tgagccagtg
9841 agttgattgc agtccagtta cgctggagtc aagctaatac gactcactat agacgaacaa
9901 tgtttgtttt tcttgtttta ttgccactag tctctagtca gtgtgttaat cttacaacca
9961 gaactcaatt accccctgca tacactaatt ctttcacacg tggtgtttat taccctgaca
10021 aagttttcag atcctcagtt ttacattcaa ctcaggactt gttcttacct ttcttttcca
10081 atgttacttg gttccatgct atacatgtct ctgggaccaa tggtactaag aggttttaga
10141 accctgtcct accatttaat gatggtgttt attttgcttc cactgagaag tctaacataa
10201 taagaggctg gatttttggt actactttag attcgaagac ccagtcccta cttattgtta
10261 ataacgctac taatgttgtt attaaagtct gtgaatttca attttgtaat gatccatttt
10321 tgggtgttta ttaccacaaa aacaacaaaa gttgg
//
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