质粒名称: | pLM-CMV-R-Cre |
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出品公司: | Addgene |
目录编号: | 27546 |
存储实验室: | Michel Sadelain |
载体骨架基本信息 |
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载体名称: | pLM (Sadelain lab) |
载体出品公司: | -- |
空载体大小: | 6711 bp |
完整质粒大小: | -- |
载体修饰: | -- |
载体类型: | 慢病毒载体,Cre/Lox |
筛选标记: | -- |
载体抗性: | 氨苄霉素100 μg/mL |
生长温度: | 37 ℃ |
宿主菌: | Stbl3 |
拷贝数: | 高拷贝 |
插入基因信息 |
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插入基因名称: | mCherry_P2A_Cre recombinase |
物种来源: | -- |
插入大小: | 1806 bp |
基因突变: | -- |
GenBank编号 | -- |
启动子: | -- |
标签/融合蛋白: | -- |
克隆方法: | 限制性内切酶 |
5’克隆位点: | AgeI(在克隆过程中销毁) |
3’克隆位点: | SalI (未破坏) |
5’测序引物 | -- |
3’测序引物 | -- |
文献引用方法 |
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材料和方法部分: | pLM-CMV-R-Cre was a gift from Michel Sadelain (Addgene plasmid # 27546 ; http://n2t.net/addgene:27546 ; RRID:Addgene_27546) |
参考文献部分: | Genomic safe harbors permit high I. Papapetrou EP, Lee G, Malani N, Setty M, Riviere I, Tirunagari LM, Kadota K, Roth SL, Giardina P, Viale A, Leslie C, Bushman FD, Studer L, Sadelain M. Nat Biotechnol. 2010 Dec 12. ():. 10.1038/nbt.1717 PubMed 21151124 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLM-CMV-R-Cre 8522 bp ds-DNA circular SYN 10-JUN-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pLM-CMV-R-Cre SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8522) AUTHORS Papapetrou EP, Lee G, Malani N, Setty M, Riviere I, Tirunagari LM, Kadota K, Roth SL, Giardina P, Viale A, Leslie C, Bushman FD, Studer L, Sadelain M TITLE Genomic safe harbors permit high I JOURNAL Nat Biotechnol. 2010 Dec 12. ():. PUBMED 21151124 REFERENCE 2 (bases 1 to 8522) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 10, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8522 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 40..57 /label=L4440 /note="L4440 vector, forward primer" protein_bind 174..195 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 210..240 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 248..264 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 253..275 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 272..288 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 272..288 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 307..327 /label=T3 /note="T3 promoter, forward primer" promoter 309..327 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 355..581 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 582..762 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 809..934 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1427..1660 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1845..1889 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2156..2273 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" enhancer 2350..2653 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 2654..2857 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 2808..2828 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" CDS 2895..3602 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /label=mCherry /note="mammalian codon-optimized" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" primer_bind complement(3040..3058) /label=mCherry-R /note="mCherry, reverse primer" primer_bind 3308..3327 /label=mCherry-F /note="mCherry, forward primer" CDS 3612..3668 /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /label=P2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="ATNFSLLKQAGDVEENPGP" CDS 3669..4700 /codon_start=1 /product="site-specific recombinase" /label=Cre /note="Cre is a site-specific recombinase from bacteriophage P1. Recombination occurs at loxP sequences." /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE DGD" misc_feature 4707..5295 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(4760..4780) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(5178..5189) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 5448..5628 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" polyA_signal 5700..5821 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(5737..5756) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 5791..5810 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 5861..5996 /label=SV40 ori /note="SV40 origin of replication" primer_bind 5923..5942 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(6016..6035) /label=T7 /note="T7 promoter, forward primer" promoter complement(6017..6035) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(6045..6062) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(6045..6061) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6054..6076) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 6203..6658 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(6290..6309) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 6500..6521 /label=F1ori-F /note="F1 origin, forward primer" promoter 6684..6788 /gene="bla" /label=AmpR promoter CDS 6789..7649 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(7007..7026) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 7820..8408 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 8309..8328 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa 61 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc 121 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg 181 agttagctca ctcattaggc accccaggtt ttacacttta tgcttccggc tcgtatgttg 241 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc 301 aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt 361 cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac 421 aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct 481 tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt 541 gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc 601 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa 661 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga 721 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga 781 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc 841 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg 901 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc 961 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat 1021 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc 1081 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga 1141 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat 1201 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac 1261 cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga 1321 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca 1381 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc 1441 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac 1501 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg 1561 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa 1621 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg 1681 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg 1741 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa 1801 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat 1861 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt 1921 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg 1981 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc 2041 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga 2101 gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgg ttaactttta 2161 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa 2221 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttatcgatc 2281 acgagactag cctcgataat agtaatcaat tacggggtca ttagttcata gcccatatat 2341 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc 2401 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca 2461 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta 2521 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta 2581 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat 2641 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga 2701 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca 2761 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg 2821 taggcgtgta cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg 2881 ctagcgctac cggtatggtg agcaagggcg aggaggataa catggccatc atcaaggagt 2941 tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg 3001 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg 3061 gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg 3121 cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct 3181 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact 3241 cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct 3301 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt 3361 accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg 3421 gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg 3481 gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg 3541 tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca 3601 agggatctgg agcaacaaac ttctcactac tcaaacaagc aggtgacgtg gaggagaatc 3661 ccggccctat gtccaattta ctgaccgtac accaaaattt gcctgcatta ccggtcgatg 3721 caacgagtga tgaggttcgc aagaacctga tggacatgtt cagggatcgc caggcgtttt 3781 ctgagcatac ctggaaaatg cttctgtccg tttgccggtc gtgggcggca tggtgcaagt 3841 tgaataaccg gaaatggttt cccgcagaac ctgaagatgt tcgcgattat cttctatatc 3901 ttcaggcgcg cggtctggca gtaaaaacta tccagcaaca tttgggccag ctaaacatgc 3961 ttcatcgtcg gtccgggctg ccacgaccaa gtgacagcaa tgctgtttca ctggttatgc 4021 ggcggatccg aaaagaaaac gttgatgccg gtgaacgtgc aaaacaggct ctagcgttcg 4081 aacgcactga tttcgaccag gttcgttcac tcatggaaaa tagcgatcgc tgccaggata 4141 tacgtaatct ggcatttctg gggattgctt ataacaccct gttacgtata gccgaaattg 4201 ccaggatcag ggttaaagat atctcacgta ctgacggtgg gagaatgtta atccatattg 4261 gcagaacgaa aacgctggtt agcaccgcag gtgtagagaa ggcacttagc ctgggggtaa 4321 ctaaactggt cgagcgatgg atttccgtct ctggtgtagc tgatgatccg aataactacc 4381 tgttttgccg ggtcagaaaa aatggtgttg ccgcgccatc tgccaccagc cagctatcaa 4441 ctcgcgccct ggaagggatt tttgaagcaa ctcatcgatt gatttacggc gctaaggatg 4501 actctggtca gagatacctg gcctggtctg gacacagtgc ccgtgtcgga gccgcgcgag 4561 atatggcccg cgctggagtt tcaataccgg agatcatgca agctggtggc tggaccaatg 4621 taaatattgt catgaactat atccgtaacc tggatagtga aacaggggca atggtgcgcc 4681 tgctggaaga tggcgattag gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat 4741 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc 4801 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct 4861 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca 4921 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt 4981 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg 5041 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga 5101 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt 5161 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc 5221 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt 5281 gggccgcctc cccgcctgga attcgagctc ggtaccttta agaccaatga cttacaaggc 5341 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc 5401 ccaacgaaga caagatatcc tgctagctcc ggatccggac ctgtactggg tctctctggt 5461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc 5521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta 5581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat 5641 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga 5701 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa 5761 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt 5821 atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc 5881 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga 5941 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg 6001 gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta 6061 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc 6121 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg 6181 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg 6241 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct 6301 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg 6361 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag 6421 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg 6481 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc 6541 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat 6601 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag 6661 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 6721 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 6781 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt 6841 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt 6901 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt 6961 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg 7021 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga 7081 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa 7141 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga 7201 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa 7261 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca 7321 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta 7381 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac 7441 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc 7501 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag 7561 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga 7621 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt 7681 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 7741 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 7801 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 7861 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 7921 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc 7981 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 8041 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 8101 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 8161 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 8221 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 8281 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 8341 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 8401 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 8461 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg 8521 ag //