| 质粒名称: | pZMU-Dest |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 105089 |
| 存储实验室: | Christopher Grefen |
|
载体骨架基本信息 |
|
| 载体名称: | pZMU-Dest |
| 载体出品公司: | -- |
| 载体抗性: | 氯霉素和大观霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 酵母表达 |
| 筛选标记: | URA3 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | myc（主干上的C终端） |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pZMU-Dest was a gift from Christopher Grefen (Addgene plasmid # 105089 ; http://n2t.net/addgene:105089 ; RRID:Addgene_105089) |
| 参考文献部分: | The split-ubiquitin system for the analysis of three-component interactions. Grefen C. Methods Mol Biol. 2014;1062:659-78. doi: 10.1007/978-1-62703-580-4_34. 10.1007/978-1-62703-580-4_34 PubMed 24057391 |
买家导航
pZMUDest载体基本信息
pZMUDest载体图谱质粒图谱和多克隆位点信息
pZMUDest载体简介
pZMUDest载体序列
LOCUS pZMU-Dest 8679 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Yeast expression vector for Gateway cloning and SUS bridge assays.
ACCESSION .
VERSION .
KEYWORDS pZMU-Dest
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8679)
AUTHORS Grefen C
TITLE The split-ubiquitin system for the analysis of three-component
interactions.
JOURNAL Methods Mol Biol. 2014;1062:659-78. doi:
10.1007/978-1-62703-580-4_34.
PUBMED 24057391
REFERENCE 2 (bases 1 to 8679)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8679
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 1..397
/gene="S. cerevisiae ADH1"
/label=ADH1 promoter
/note="promoter for alcohol dehydrogenase 1"
protein_bind 424..548
/gene="mutant version of attR"
/label=attR1
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
promoter 573..603
/label=lac UV5 promoter
/note="E. coli lac promoter with an ""up"" mutation"
CDS 657..1316
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
primer_bind complement(723..742)
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
CDS 1658..1963
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/label=ccdB
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
primer_bind 1842..1861
/label=ccdB-fwd
/note="ccdB gene, forward primer"
protein_bind complement(2004..2128)
/gene="mutant version of attR"
/label=attR2
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
CDS 2151..2180
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
terminator 2323..2510
/gene="S. cerevisiae ADH1"
/label=ADH1 terminator
/note="transcription terminator for the S. cerevisiae
alcohol dehydrogenase 1 (ADH1) gene"
primer_bind complement(2378..2402)
/label=pHybLex-R
/note="ADH terminator, reverse primer"
rep_origin 2521..3867
/label=2u ori
/note="yeast 2u plasmid origin of replication"
CDS complement(4547..5350)
/codon_start=1
/gene="S. cerevisiae URA3"
/product="orotidine-5'-phosphate decarboxylase, required
for uracil biosynthesis"
/label=URA3
/note="yeast auxotrophic marker, counterselectable with
5-fluoroorotic acid (5-FOA)"
/translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
promoter complement(5351..5562)
/gene="S. cerevisiae URA3"
/label=URA3 promoter
rep_origin complement(6156..6669)
/direction=LEFT
/label=M13 ori
/note="M13 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6351..6372)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 6563..6582
/label=F1ori-R
/note="F1 origin, reverse primer"
CDS 7009..7800
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase (Murphy,
1985)"
/label=SmR
/note="confers resistance to spectinomycin and
streptomycin"
/translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
rep_origin 8046..8634
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 8535..8554
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 caacttcttt tctttttttt tcttttctct ctcccccgtt gttgtctcac catatccgca
61 atgacaaaaa aatgatggaa gacactaaag gaaaaaatta acgacaaaga cagcaccaac
121 agatgtcgtt gttccagagc tgatgagggg tatctcgaag cacacgaaac tttttccttc
181 cttcattcac gcacactact ctctaatgag caacggtata cggccttcct tccagttact
241 tgaatttgaa ataaaaaaaa gtttgctgtc ttgctatcaa gtataaatag acctgcaatt
301 attaatcttt tgtttcctcg tcattgttct cgttcccttt cttccttgtt tctttttctg
361 cacaatattt caagctatac caagcataca atcaactcca agctggatcc tctagacgag
421 ctcacaagtt tgtacaaaaa agctgaacga gaaacgtaaa atgatataaa tatcaatata
481 ttaaattaga ttttgcataa aaaacagact acataatact gtaaaacaca acatatccag
541 tcactatggc ggccgcatta ggcaccccag gctttacact ttatgcttcc ggctcgtata
601 atgtgtggat tttgagttag gatccgtcga gattttcagg agctaaggaa gctaaaatgg
661 agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt
721 ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta
781 cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca
841 ttcttgcccg cctgatgaat gctcatccgg aattccgtat ggcaatgaaa gacggtgagc
901 tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt
961 tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc
1021 aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata
1081 tgtttttcgt ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca
1141 atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca
1201 aggtgctgat gccgctggcg attcaggttc atcatgccgt ttgtgatggc ttccatgtcg
1261 gcagaatgct taatgaatta caacagtact gcgatgagtg gcagggcggg gcgtaaacgc
1321 gtggatccgg cttactaaaa gccagataac agtatgcgta tttgcgcgct gatttttgcg
1381 gtataagaat atatactgat atgtataccc gaagtatgtc aaaaagaggt atgctatgaa
1441 gcagcgtatt acagtgacag ttgacagcga cagctatcag ttgctcaagg catatatgat
1501 gtcaatatct ccggtctggt aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc
1561 gaacgctgga aagcggaaaa tcaggaaggg atggctgagg tcgcccggtt tattgaaatg
1621 aacggctctt ttgctgacga gaacaggggc tggtgaaatg cagtttaagg tttacaccta
1681 taaaagagag agccgttatc gtctgtttgt ggatgtacag agtgatatta ttgacacgcc
1741 cgggcgacgg atggtgatcc ccctggccag tgcacgtctg ctgtcagata aagtctcccg
1801 tgaactttac ccggtggtgc atatcgggga tgaaagctgg cgcatgatga ccaccgatat
1861 ggccagtgtg ccggtctccg ttatcgggga agaagtggct gatctcagcc accgcgaaaa
1921 tgacatcaaa aacgccatta acctgatgtt ctggggaata taaatgtcag gctcccttat
1981 acacagccag tctgcaggtc gaccatagtg actggatatg ttgtgtttta cagtattatg
2041 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt
2101 ctcgttcagc tttcttgtac aaagtggtga ctcgagccgc catcatggag gagcagaagc
2161 tgatctcaga ggaggacctg cactgaagct ttggacttct tcgccagagg tttggtcaag
2221 tctccaatca aggttgtcgg cttgtctacc ttgccagaaa tttacgaaaa gatggaaaag
2281 ggtcaaatcg ttggtagata cgttgttgac acttctaaat aagcgaattt cttatgattt
2341 atgattttta ttattaaata agttataaaa aaaataagtg tatacaaatt ttaaagtgac
2401 tcttaggttt taaaacgaaa attcttattc ttgagtaact ctttcctgta ggtcaggttg
2461 ctttctcagg tatagcatga ggtcgctctt attgaccaca cctctaccgg catgcgatat
2521 gatccaatat caaaggaaat gatagcattg aaggatgaga ctaatccaat tgaggagtgg
2581 cagcatatag aacagctaaa gggtagtgct gaaggaagca tacgataccc cgcatggaat
2641 gggataatat cacaggaggt actagactac ctttcatcct acataaatag acgcatataa
2701 gtacgcattt aagcataaac acgcactatg ccgttcttct catgtatata tatatacagg
2761 caacacgcag atataggtgc gacgtgaaca gtgagctgta tgtgcgcagc tcgcgttgca
2821 ttttcggaag cgctcgtttt cggaaacgct ttgaagttcc tattccgaag ttcctattct
2881 ctagctagaa agtataggaa cttcagagcg cttttgaaaa ccaaaagcgc tctgaagacg
2941 cactttcaaa aaaccaaaaa cgcaccggac tgtaacgagc tactaaaata ttgcgaatac
3001 cgcttccaca aacattgctc aaaagtatct ctttgctata tatctctgtg ctatatccct
3061 atataaccta cccatccacc tttcgctcct tgaacttgca tctaaactcg acctctacat
3121 tttttatgtt tatctctagt attactcttt agacaaaaaa attgtagtaa gaactattca
3181 tagagtgaat cgaaaacaat acgaaaatgt aaacatttcc tatacgtagt atatagagac
3241 aaaatagaag aaaccgttca taattttctg accaatgaag aatcatcaac gctatcactt
3301 tctgttcaca aagtatgcgc aatccacatc ggtatagaat ataatcgggg atgcctttat
3361 cttgaaaaaa tgcacccgca gcttcgctag taatcagtaa acgcgggaag tggagtcagg
3421 ctttttttat ggaagagaaa atagacacca aagtagcctt cttctaacct taacggacct
3481 acagtgcaaa aagttatcaa gagactgcat tatagagcgc acaaaggaga aaaaaagtaa
3541 tctaagatgc tttgttagaa aaatagcgct ctcgggatgc atttttgtag aacaaaaaag
3601 aagtatagat tctttgttgg taaaatagcg ctctcgcgtt gcatttctgt tctgtaaaaa
3661 tgcagctcag attctttgtt tgaaaaatta gcgctctcgc gttgcatttt tgttttacaa
3721 aaatgaagca cagattcttc gttggtaaaa tagcgctttc gcgttgcatt tctgttctgt
3781 aaaaatgcag ctcagattct ttgtttgaaa aattagcgct ctcgcgttgc atttttgttc
3841 tacaaaatga agcacagatg cttcgttaac aaagatatgc tattgaagtg caagatggaa
3901 acgcagaaaa tgaaccgggg atgcgacgtg caagattacc tatgcaatag atgcaatagt
3961 ttctccagga accgaaatac atacattgtc ttccgtaaag cgctagacta tatattatta
4021 tacaggttca aatatactat ctgtttcagg gaaaactccc aggttcggat gttcaaaatt
4081 caatgatggg taacaagtac gatcgtaaat ctgtaaaaca gtttgtcgga tattaggctg
4141 tatctcctca aagcgtattc gaatatcatt gagaagctgc attttttttt tttttttttt
4201 tttttttttt tatatatatt tcaaggatat accattgtaa tgtctgcccc taagaagatc
4261 gtcgttttgc caggtgacca cgttggtcaa gaaatcacag ccgaagccat taaggttctt
4321 aaagctattt ctgatgttcg ttccaatgtc aagttcgatt tcgaaaatca tttaattggt
4381 ggtgctgcta tcgcagatcc agatctggct ttttctttcc aatttttttt ttttcgtcat
4441 tataaaaatc attacgaccg agattcccgg gtaataactg atataattaa attgaagctc
4501 taatttgtga gtttagtata catgcattta cttataatac agttttttag ttttgctggc
4561 cgcatcttct caaatatgct tcccagcctg cttttctgta acgttcaccc tctaccttag
4621 catcccttcc ctttgcaaat agtcctcttc caacaataat aatgtcagat cctgtagaga
4681 ccacatcatc cacggttcta tactgttgac ccaatgcgtc tcccttgtca tctaaaccca
4741 caccgggtgt cataatcaac caatcgtaac cttcatctct tccacccatg tctctttgag
4801 caataaagcc gataacaaaa tctttgtcgc tcttcgcaat gtcaacagta cccttagtat
4861 attctccagt agatagggag cccttgcatg acaattctgc taacatcaaa aggcctctag
4921 gttcctttgt tacttcttct gccgcctgct tcaaaccgct aacaatacct gggcccacca
4981 caccgtgtgc attcgtaatg tctgcccatt ctgctattct gtatacaccc gcagagtact
5041 gcaatttgac tgtattacca atgtcagcaa attttctgtc ttcgaagagt aaaaaattgt
5101 acttggcgga taatgccttt agcggcttaa ctgtgccctc catggaaaaa tcagtcaaga
5161 tatccacatg tgtttttagt aaacaaattt tgggacctaa tgcttcaact aactccagta
5221 attccttggt ggtacgaaca tccaatgaag cacacaagtt tgtttgcttt tcgtgcatga
5281 tattaaatag cttggcagca acaggactag gatgagtagc agcacgttcc ttatatgtag
5341 ctttcgacat gatttatctt cgtttccggt ttttgttctg tgcagttggg ttaagaatac
5401 tgggcaattt catgtttctt caacactaca tatgcgtata tataccaatc taagtctgtg
5461 ctccttcctt cgttcttcct tctgttcgga gattaccgaa tcaaaaaaat ttcaaggaaa
5521 ccgaaatcaa aaaaaagaat aaaaaaaaaa tgatgaattg aaaagccaga tctgatcatc
5581 tccgatgaag cctccgttat cccaggctcc ttgggtttgt tgccatctgc gtccttggcc
5641 tctttgccag acaagaacac cgcatttggt ttgtacgaac catgccatgg ttccgctcca
5701 gatttgccaa agaataaggt caaccctatc gccactatct tgtctgctgc aatgatgttg
5761 aaattgtcat tgaacttgcc tgaagaaggt aaagccattg aagatgcagt taaaaaggtt
5821 ttggatgcag gtatcagaac tggtgattta ggtggttcca acagtaccac cgaagtcggt
5881 gatgctgtcg ccgaagaagt taagaaaatc cttgcttaaa aagattctct ttttttatga
5941 tatttgtama aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaat gcagcgtcac
6001 atcggataat aatgatggca gccattgtag aagtgccttt tgcatttcta gtctctttct
6061 cggtctagct agttttacta catcgcgaag atagaatctt agatcacact gcctttgctg
6121 agctggatca atagagtaac aaaagagtgg taaggacccc ggttgataat cagaaaagcc
6181 ccaaaaacag gaagattgta taagcaaata tttaaattgt aagcgttaat attttgttaa
6241 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
6301 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
6361 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
6421 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
6481 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
6541 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
6601 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
6661 agggcgcgtc ctcgttaaag gacaaggacc tgagcggaag tgtatcgtac agtagacgga
6721 gtatactagt aggcacgaac ccagtggaca taagcctgtt cggttcgtaa gctgtaatgc
6781 aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac
6841 ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttggggtaca gtctatgcct
6901 cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc
6961 aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacatcat gggggaagcg
7021 gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc
7081 gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca
7141 cacagtgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga
7201 gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc
7261 gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag
7321 cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca
7381 gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc
7441 ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag
7501 gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga
7561 aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg
7621 aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata
7681 cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat
7741 cagttggaag aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa
7801 tgtctagcta gaaattcgtt caagccgacg ccgcttcgcg gcgcggctta actcaagcgt
7861 tagatgcact aagcacataa ttgctcacag ccaaactatc aggtcaagtc tgcttttatt
7921 atttttaagc gtgcataata agccctacac aaattgggag atatatcatg catgaccaaa
7981 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
8041 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
8101 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
8161 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
8221 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
8281 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
8341 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
8401 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
8461 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
8521 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
8581 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
8641 agcaacgcgg cctttttacg gttcctggcc ttttgcatg
//
