质粒名称: | FH-TET3-pEF |
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出品公司: | 爱迪基因 |
目录编号: | 49446 |
存储实验室: | Anjana Rao |
载体骨架基本信息 |
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载体名称: | pEF1a |
载体出品公司: | Invitrogen |
载体抗性: | 氨苄霉素 |
空载体大小: | 6200 bp |
完整质粒大小: | 11669 bp |
载体类型: | 哺乳细胞表达 |
筛选标记: | 新霉素(G418) |
高拷贝/低拷贝: | 未知 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | Tet methylcytosine dioxygenase 3 |
物种来源: | H. sapiens (human) |
插入大小: | 5469 bp |
启动子 | EF-1alpha |
标签/融合蛋白: | Flag(N端) HA(N端) |
克隆方法: | 限制性内切酶 |
5' 克隆位点: | KpnI(未破坏) |
3' 克隆位点: | XbaI(未破坏) |
5' 测序引物 | T7 promoter |
3' 测序引物 | BGH Reverse |
文献引用方法 |
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材料和方法部分: | FH-TET3-pEF was a gift from Anjana Rao (Addgene plasmid # 49446) |
参考文献部分: | Modulation of TET2 expression and 5-methylcytosine oxidation by the CXXC domain protein IDAX. Ko M, An J, Bandukwala HS, Chavez L, Aijo T, Pastor WA, Segal MF, Li H, Koh KP, Lahdesmaki H, Hogan PG, Aravind L, Rao A. Nature. 2013 May 2;497(7447):122-6. doi: 10.1038/nature12052. Epub 2013 Apr 7. 10.1038/nature12052 PubMed 23563267 |
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载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS FH-TET3-pEF Sequencing Result 11575 bp DNA SYN DEFINITION FH-TET3-pEF Sequencing Result ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..11575 /organism="FH-TET3-pEF Sequencing Result" /mol_type="other DNA" promoter 359..1530 /label="EF1a_promoter" CDS complement(650..1270) /label="ORF frame 1" misc_feature 1478..1498 /label="EF1a_fwd_primer" promoter 1547..1565 /label="T7_promoter" CDS 1604..7066 /label="ORF frame 2" misc_feature 1607..1630 /label="FLAG" misc_feature 1643..1669 /label="HA" CDS complement(4388..5602) /label="ORF frame 1" CDS complement(5974..6744) /label="ORF frame 2" CDS complement(6470..7153) /label="ORF frame 1" misc_feature complement(7176..7193) /label="BGH_rev_primer" terminator 7179..7406 /label="bGH_PA_terminator" rep_origin 7469..7775 /label="f1_origin" misc_feature complement(7889..7909) /label="pBABE_3_primer" misc_feature complement(7895..8110) /label="SV40_enhancer" promoter 7907..8175 /label="SV40_promoter" rep_origin 8074..8151 /label="SV40_origin" misc_feature 8136..8155 /label="SV40pro_F_primer" CDS 8290..9084 /label="ORF frame 1" gene 8293..9081 /label="NeoR/KanR" /gene="NeoR/KanR" terminator 9263..9382 /label="SV40_PA_terminator" misc_feature 9351..9370 /label="EBV_rev_primer" promoter complement(9426..9444) /label="M13_reverse_primer" misc_feature complement(9443..9465) /label="M13_pUC_rev_primer" promoter complement(9479..9508) /label="lac_promoter" gene complement(10580..11440) /label="Ampicillin" /gene="Ampicillin" CDS complement(10580..11440) /label="ORF frame 1" promoter complement(11482..11510) /label="AmpR_promoter" ORIGIN 1 cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat ctGCTCTGAT 61 GCCGCATAGT TAAGCCAGTA TCTGCTCCCT GCTTGTGTGT TGGAGGTCGC TGAGTAGTGC 121 GCGAGCAAAA TTTAAGCTAC AACAAGGCAA GGCTTGACCG ACAATTGCAT GAAGAATCTG 181 CTTAGGGTTA GGCGTTTTGC GCTGCTTCGC GATGTACGGG CCAGATATAC GCGTTGACAT 241 TGATTATTGA CTAGGCTTTT GCAAAAAGCT TTGCAAAGAT GGATAAAGTT TTAAACAGAG 301 AGGAATCTTT GCAGCTAATG GACCTTCTAG GTCTTGAAAG GAGTGGGAAT TGGCTCCGGT 361 GCCCGTCAGT GGGCAGAGCG CACATCGCCC ACAGTCCCCG AGAAGTTGGG GGGAGGGGTC 421 GGCAATTGAA CCGGTGCCTA GAGAAGGTGG CGCGGGGTAA ACTGGGAAAG TGATGTCGTG 481 TACTGGCTCC GCCTTTTTCC CGAGGGTGGG GGAGAACCGT ATATAAGTGC AGTAGTCGCC 541 GTGAACGTTC TTTTTCGCAA CGGGTTTGCC GCCAGAACAC AGGTAAGTGC CGTGTGTGGT 601 TCCCGCGGGC CTGGCCTCTT TACGGGTTAT GGCCCTTGCG TGCCTTGAAT TACTTCCACC 661 TGGCTGCAGT ACGTGATTCT TGATCCCGAG CTTCGGGTTG GAAGTGGGTG GGAGAGTTCG 721 AGGCCTTGCG CTTAAGGAGC CCCTTCGCCT CGTGCTTGAG TTGAGGCCTG GCCTGGGCGC 781 TGGGGCCGCC GCGTGCGAAT CTGGTGGCAC CTTCGCGCCT GTCTCGCTGC TTTCGATAAG 841 TCTCTAGCCA TTTAAAATTT TTGATGACCT GCTGCGACGC TTTTTTTCTG GCAAGATAGT 901 CTTGTAAATG CGGGCCAAGA TCTGCACACT GGTATTTCGG TTTTTGGGGC CGCGGGCGGC 961 GACGGGGCCC GTGCGTCCCA GCGCACATGT TCGGCGAGGC GGGGCCTGCG AGCGCGGCCA 1021 CCGAGAATCG GACGGGGGTA GTCTCAAGCT GGCCGGCCTG CTCTGGTGCC TGGCCTCGCG 1081 CCGCCGTGTA TCGCCCCGCC CTGGGCGGCA AGGCTGGCCC GGTCGGCACC AGTTGCGTGA 1141 GCGGAAAGAT GGCCGCTTCC CGGCCCTGCT GCAGGGAGCT CAAAATGGAG GACGCGGCGC 1201 TCGGGAGAGC GGGCGGGTGA GTCACCCACA CAAAGGAAAA GGGCCTTTCC GTCCTCAGCC 1261 GTCGCTTCAT GTGACTCCAC GGAGTACCGG GCGCCGTCCA GGCACCTCGA TTAGTTCTCG 1321 AGCTTTTGGA GTACGTCGTC TTTAGGTTGG GGGGAGGGGT TTTATGCGAT GGAGTTTCCC 1381 CACACTGAGT GGGTGGAGAC TGAAGTTAGG CCAGCTTGGC ACTTGATGTA ATTCTCCTTG 1441 GAATTTGCCC TTTTTGAGTT TGGATCTTGG TTCATTCTCA AGCCTCAGAC AGTGGTTCAA 1501 AGTTTTTTTC TTCCATTTCA GGTGTCGTGA GGAATTAGCT TGGTACTAAT ACGACTCACT 1561 ATAGGGAGAC CCAAGCTGGC TAGGTAAGCT TGGTACCGCC ACCATGGACT ACAAGGACGA 1621 CGATGACAAG CTCGATGGAG GATACCCCTA CGACGTGCCC GACTACGCCG GAGGACTCGA 1681 CAGCCAGTTT CAGGTGCCCC TGGCCGTCCA GCCGGACCTG CCAGGCCTTT ATGACTTCCC 1741 TCAGCGCCAG GTGATGGTAG GGAGCTTCCC GGGGTCTGGG CTCTCCATGG CTGGGAGTGA 1801 GTCCCAACTC CGAGGGGGTG GAGATGGTCG AAAGAAACGG AAACGGTGTG GTACTTGTGA 1861 GCCCTGCCGG CGGCTGGAAA ACTGTGGCGC TTGCACTAGC TGTACCAACC GCCGCACGCA 1921 CCAGATCTGC AAACTGCGAA AATGTGAGGT GCTGAAGAAA AAAGTAGGGC TTCTCAAGGA 1981 GGTGGAAATA AAGGCTGGTG AAGGAGCCGG GCCGTGGGGA CAAGGAGCGG CTGTCAAGAC 2041 AGGCTCAGAG CTCAGCCCAG TTGATGGACC TGTTCCAGGT CAGATGGACT CAGGGCCAGT 2101 GTACCATGGG GACTCACGGC AGCTAAGCGC CTCAGGGGTG CCGGTCAATG GTGCTAGAGA 2161 GCCCGCTGGA CCCAGTCTGC TGGGGACTGG GGGTCCTTGG CGGGTAGACC AAAAGCCCGA 2221 CTGGGAGGCT GCCCCAGGCC CAGCTCATAC TGCTCGCCTG GAAGATGCCC ACGATCTGGT 2281 GGCCTTTTCG GCTGTGGCCG AAGCTGTGTC CTCTTATGGG GCCCTTAGCA CCCGGCTCTA 2341 TGAAACCTTC AACCGTGAGA TGAGTCGTGA GGCTGGGAAC AACAGCAGGG GACCCCGGCC 2401 AGGGCCTGAG GGCTGCTCTG CTGGCAGCGA AGACCTTGAC ACACTGCAGA CGGCCCTGGC 2461 CCTCGCGCGG CATGGTATGA AACCACCCAA CTGCAACTGC GATGGCCCAG AATGCCCTGA 2521 CTACCTCGAG TGGCTGGAGG GGAAGATCAA GTCTGTGGTC ATGGAAGGAG GGGAGGAGCG 2581 GCCCAGGCTC CCAGGGCCTC TGCCTCCTGG TGAGGCCGGC CTCCCAGCAC CAAGCACCAG 2641 GCCACTCCTC AGCTCAGAGG TGCCCCAGAT CTCTCCCCAA GAGGGCCTGC CCCTGTCCCA 2701 GAGTGCCCTG AGCATTGCCA AGGAAAAAAA CATCAGCTTG CAGACCGCCA TTGCCATTGA 2761 GGCCCTCACA CAGCTCTCCT CTGCCCTCCC GCAGCCTTCT CATTCCACCC CCCAGGCTTC 2821 TTGCCCCCTT CCTGAGGCCT TGTCACCTCC TGCCCCTTTC AGATCTCCCC AGTCTTACCT 2881 CCGGGCTCCC TCATGGCCTG TGGTTCCTCC TGAAGAGCAC TCATCTTTTG CTCCTGATAG 2941 CTCTGCCTTC CCTCCAGCAA CTCCTAGAAC TGAGTTCCCT GAAGCCTGGG GCACTGACAC 3001 CCCTCCAGCA ACGCCCCGGA GCTCCTGGCC CATGCCTCGC CCAAGCCCCG ATCCCATGGC 3061 TGAACTGGAG CAGTTGTTGG GCAGCGCCAG TGATTACATC CAGTCAGTAT TCAAGCGGCC 3121 TGAGGCCCTG CCTACCAAGC CCAAGGTCAA GGTGGAGGCA CCCTCTTCCT CCCCGGCCCC 3181 GGCCCCATCC CCTGTACTTC AGAGGGAGGC TCCCACGCCA TCCTCGGAGC CCGACACCCA 3241 CCAGAAGGCC CAGACCGCCC TGCAGCAGCA CCTCCACCAC AAGCGCAGCC TCTTCCTAGA 3301 ACAGGTGCAC GACACCTCCT TCCCTGCTCC TTCAGAGCCT TCTGCTCCTG GCTGGTGGCC 3361 CCCACCAAGT TCACCTGTCC CACGGCTTCC AGACAGACCA CCCAAGGAGA AGAAGAAGAA 3421 GCTCCCAACA CCAGCTGGAG GTCCCGTGGG AACGGAGAAA GCTGCCCCTG GGATCAAGCC 3481 CAGTGTCCGA AAGCCCATTC AGATCAAGAA GTCCAGGCCC CGGGAAGCAC AGCCCCTCTT 3541 CCCACCTGTC CGACAGATTG TCCTGGAAGG GCTTAGGTCC CCAGCCTCCC AGGAAGTGCA 3601 GGCTCATCCA CCGGCCCCTC TGCCTGCCTC ACAGGGCTCT GCTGTGCCCC TGCCCCCAGA 3661 ACCTTCTCTT GCGCTATTTG CACCTAGTCC CTCCAGGGAC AGCCTGCTGC CCCCTACTCA 3721 GGAAATGAGG TCCCCCAGCC CCATGACAGC CTTGCAGCCA GGCTCCACTG GCCCTCTTCC 3781 CCCTGCCGAT GACAAGCTGG AAGAGCTCAT CCGGCAGTTT GAGGCTGAAT TTGGAGATAG 3841 CTTTGGGCTT CCCGGCCCCC CTTCTGTGCC CATTCAGGAC CCCGAGAACC AGCAAACATG 3901 TCTCCCAGCC CCTGAGAGCC CCTTTGCTAC CCGTTCCCCC AAGCAAATCA AGATTGAGTC 3961 TTCGGGGGCT GTGACTGTGC TCTCAACCAC CTGCTTCCAT TCAGAGGAGG GAGGACAGGA 4021 GGCCACACCC ACCAAGGCTG AGAACCCACT CACACCCACC CTCAGTGGCT TCTTGGAGTC 4081 ACCTCTTAAG TACCTGGACA CACCCACCAA GAGTCTGCTG GACACACCTG CCAAGAGAGC 4141 CCAGGCCGAG TTCCCCACCT GCGATTGCGT CGAACAAATA GTGGAGAAAG ATGAAGGTCC 4201 ATATTATACT CACTTGGGAT CTGGCCCCAC GGTCGCCTCT ATCCGGGAAC TCATGGAGGA 4261 GCGGTATGGA GAGAAGGGGA AAGCCATCCG GATCGAGAAG GTCATCTACA CGGGGAAGGA 4321 GGGAAAGAGC TCCCGCGGTT GCCCCATTGC AAAGTGGGTG ATCCGCAGGC ACACGCTGGA 4381 GGAGAAGCTA CTCTGCCTGG TGCGGCACCG GGCAGGCCAC CACTGCCAGA ACGCTGTGAT 4441 CGTCATCCTC ATCCTGGCCT GGGAGGGCAT TCCCCGTAGC CTCGGAGACA CCCTCTACCA 4501 GGAGCTCACC GACACCCTCC GGAAGTATGG GAACCCCACC AGCCGGAGAT GCGGCCTCAA 4561 CGATGACCGG ACCTGCGCTT GCCAAGGCAA AGACCCCAAC ACCTGTGGTG CCTCCTTCTC 4621 CTTTGGTTGT TCCTGGAGCA TGTACTTCAA CGGCTGCAAG TATGCTCGGA GCAAGACACC 4681 TCGCAAGTTC CGCCTCGCAG GGGACAATCC CAAAGAGGAA GAAGTGCTCC GGAAGAGTTT 4741 CCAGGACCTG GCCACCGAAG TCGCTCCCCT GTACAAGCGA CTGGCCCCTC AGGCCTATCA 4801 GAACCAGGTG ACCAACGAGG AAATAGCGAT TGACTGCCGT CTGGGGCTGA AGGAAGGACG 4861 GCCCTTCGCG GGGGTCACGG CCTGCATGGA CTTCTGTGCC CACGCCCACA AGGACCAGCA 4921 TAACCTCTAC AATGGGTGCA CCGTGGTCTG CACCCTGACC AAGGAAGACA ATCGCTGCGT 4981 GGGCAAGATT CCCGAGGATG AGCAGCTGCA TGTTCTCCCC CTGTACAAGA TGGCCAACAC 5041 GGATGAGTTT GGTAGCGAGG AGAACCAGAA TGCAAAGGTG GGCAGCGGAG CCATCCAGGT 5101 GCTCACCGCC TTCCCCCGCG AGGTCCGACG CCTGCCCGAG CCTGCCAAGT CCTGCCGCCA 5161 GCGGCAGCTG GAAGCCAGAA AGGCAGCAGC CGAGAAGAAG AAGATTCAGA AGGAGAAGCT 5221 GAGCACTCCG GAGAAGATCA AGCAGGAGGC CCTGGAGCTG GCGGGCATTA CGTCGGACCC 5281 AGGCCTGTCT CTGAAGGGTG GATTGTCCCA GCAAGGCCTG AAGCCCTCCC TCAAGGTGGA 5341 GCCGCAGAAC CACTTCAGCT CCTTCAAGTA CAGCGGCAAC GCGGTGGTGG AGAGCTACTC 5401 GGTGCTGGGC AACTGCCGGC CCTCCGACCC TTACAGCATG AACAGCGTGT ACTCCTACCA 5461 CTCCTACTAT GCACAGCCCA GCCTGACCTC CGTCAATGGC TTCCACTCCA AGTACGCTCT 5521 CCCGTCTTTT AGCTACTATG GCTTTCCATC CAGCAACCCC GTCTTCCCCT CTCAGTTCCT 5581 GGGTCCTGGT GCCTGGGGGC ATAGTGGCAG CAGTGGCAGT TTTGAGAAGA AGCCAGACCT 5641 CCACGCTCTG CACAACAGCC TGAGCCCGGC CTACGGTGGT GCTGAGTTTG CCGAGCTGCC 5701 CAGCCAGGCT GTTCCCACAG ACGCCCACCA CCCCACTCCT CACCACCAGC AGCCTGCGTA 5761 CCCAGGCCCC AAGGAGTATC TGCTTCCCAA GGCCCCCCTA CTCCACTCAG TGTCCAGGGA 5821 CCCCTCCCCC TTTGCCCAGA GCTCCAACTG CTACAACAGA TCCATCAAGC AAGAGCCAGT 5881 AGACCCGCTG ACCCAGGCTG AGCCTGTGCC CAGAGACGCT GGCAAGATGG GCAAGACACC 5941 TCTGTCCGAG GTGTCTCAGA ATGGAGGACC CAGTCACCTT TGGGGACAGT ACTCAGGAGG 6001 CCCAAGCATG TCCCCCAAGA GGACTAACGG TGTGGGTGGC AGCTGGGGTG TGTTCTCGTC 6061 TGGGGAGAGT CCTGCCATCG TCCCTGACAA GCTCAGTTCC TTTGGGGCCA GCTGCCTGGC 6121 CCCTTCCCAC TTCACAGATG GCCAGTGGGG GCTGTTCCCC GGTGAGGGGC AGCAGGCAGC 6181 TTCCCACTCT GGAGGACGGC TGCGAGGCAA ACCGTGGAGC CCCTGCAAGT TTGGGAACAG 6241 CACCTCGGCC TTGGCTGGGC CCAGCCTGAC TGAGAAGCCG TGGGCGCTGG GGGCAGGGGA 6301 TTTCAACTCG GCCCTGAAAG GTAGTCCTGG GTTCCAAGAC AAGCTGTGGA ACCCCATGAA 6361 AGGAGAGGAG GGCAGGATTC CAGCCGCAGG GGCCAGCCAG CTGGACAGGG CCTGGCAGTC 6421 CTTTGGTCTG CCCCTGGGAT CCAGCGAGAA GCTGTTTGGG GCTCTGAAGT CAGAGGAGAA 6481 GCTGTGGGAC CCCTTCAGCC TGGAGGAGGG GCCGGCTGAG GAGCCCCCCA GCAAGGGAGC 6541 GGTGAAGGAG GAGAAGGGCG GTGGTGGTGC GGAGGAGGAA GAGGAGGAGC TGTGGTCGGA 6601 CAGTGAACAC AACTTCCTGG ACGAGAACAT CGGCGGCGTG GCCGTGGCCC CAGCCCACGG 6661 CTCCATCCTC ATCGAGTGTG CCCGGCGGGA GCTGCACGCC ACCACGCCGC TTAAGAAGCC 6721 CAACCGCTGC CACCCCACCC GCATCTCGCT GGTCTTCTAC CAGCACAAGA ACCTCAACCA 6781 GCCCAACCAC GGGCTGGCCC TCTGGGAAGC CAAGATGAAG CAGCTGGCGG AGAGGGCACG 6841 GGCACGGCAG GAGGAGGCTG CCCGGCTGGG CCTGGGCCAG CAGGAGGCCA AGCTCTACGG 6901 GAAGAAGCGC AAGTGGGGGG GCACTGTGGT TGCTGAGCCC CAGCAGAAAG AGAAGAAGGG 6961 GGTCGTCCCC ACCCGGCAGG CACTGGCTGT GCCCACAGAC TCGGCGGTCA CCGTGTCCTC 7021 CTATGCCTAC ACGAAGGTCA CTGGCCCCTA CAGCCGCTGG ATCTAGTCTA GAGGGCCCTT 7081 CGAAGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGCGTA CCGGTCATCA 7141 TCACCATCAC CATTGAGTTT AAACCCGCTG ATCAGCCTCG ACTGTGCCTT CTAGTTGCCA 7201 GCCATCTGTT GTTTGCCCCT CCCCCGTGCC TTCCTTGACC CTGGAAGGTG CCACTCCCAC 7261 TGTCCTTTCC TAATAAAATG AGGAAATTGC ATCGCATTGT CTGAGTAGGT GTCATTCTAT 7321 TCTGGGGGGT GGGGTGGGGC AGGACAGCAA GGGGGAGGAT TGGGAAGACA ATAGCAGGCA 7381 TGCTGGGGAT GCGGTGGGCT CTATGGCTTC TGAGGCGGAA AGAACCAGCT GGGGCTCTAG 7441 GGGGTATCCC CACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG 7501 CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC 7561 CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG 7621 GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC 7681 ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT 7741 CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC 7801 TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA 7861 ACAAAAATTT AACGCGAATT AATTCTGTGG AATGTGTGTC AGTTAGGGTG TGGAAAGTCC 7921 CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCAGG 7981 TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG 8041 TCAGCAACCA TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC 8101 GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC 8161 TCTGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC 8221 AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAAGAGAC AGGATGAGGA 8281 TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG 8341 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC 8401 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG 8461 AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC 8521 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG 8581 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT 8641 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG 8701 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT 8761 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGCGC 8821 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG 8881 GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGACCGC 8941 TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT 9001 GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT 9061 CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGCGAAATGA CCGACCAAGC 9121 GACGCCCAAC CTGCCATCAC GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG 9181 CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC CAGCGCGGGG ATCTCATGCT 9241 GGAGTTCTTC GCCCACCCCA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA 9301 TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC 9361 CAAACTCATC AATGTATCTT ATCATGTCTG TATACCGTCG ACCTCTAGCT AGAGCTTGGC 9421 GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA 9481 CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC 9541 ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA 9601 TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC 9661 CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC 9721 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 9781 AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC 9841 CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT 9901 AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC 9961 CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT 10021 CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG 10081 AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC 10141 CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA 10201 GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA 10261 GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA 10321 GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC 10381 AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG 10441 ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA 10501 TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG 10561 AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT 10621 GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG 10681 AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC 10741 CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA 10801 CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC 10861 CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT 10921 CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC 10981 CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT 11041 TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC 11101 CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT 11161 GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA 11221 GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA 11281 TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG 11341 CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA 11401 AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT 11461 ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA 11521 AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGT //
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