FH-TET3-pEF

价格:1200元

联系方式:I47-825O-882O

相关技术服务:质粒构建    基因合成    质粒大提

买家导航

载体基本信息

质粒名称: FH-TET3-pEF 
出品公司: 爱迪基因
目录编号: 49446
存储实验室: Anjana Rao 

载体骨架基本信息
载体名称: pEF1a
载体出品公司: Invitrogen
载体抗性: 氨苄霉素
空载体大小: 6200 bp
完整质粒大小: 11669 bp
载体类型: 哺乳细胞表达
筛选标记: 新霉素G418
高拷贝/低拷贝: 未知
生长条件: 37 ℃

插入基因信息
插入基因名称: Tet methylcytosine dioxygenase 3
物种来源: H. sapiens (human)
插入大小: 5469 bp
启动子 EF-1alpha
标签/融合蛋白: Flag(N端)          HA(N端)
克隆方法: 限制性内切酶
5' 克隆位点: KpnI(未破坏)
3' 克隆位点: XbaI(未破坏)
5' 测序引物 T7 promoter
3' 测序引物 BGH Reverse

文献引用方法
材料和方法部分: FH-TET3-pEF was a gift from Anjana Rao (Addgene plasmid # 49446)
参考文献部分: Modulation of TET2 expression and 5-methylcytosine oxidation by the CXXC domain protein IDAX. Ko M, An J, Bandukwala HS, Chavez L, Aijo T, Pastor WA, Segal MF, Li H, Koh KP, Lahdesmaki H, Hogan PG, Aravind L, Rao A. Nature. 2013 May 2;497(7447):122-6. doi: 10.1038/nature12052. Epub 2013 Apr 7. 10.1038/nature12052 PubMed 23563267

载体图谱质粒图谱和多克隆位点信息

FH-TET3-pEF载体图谱

载体简介

载体序列

LOCUS       FH-TET3-pEF Sequencing Result	11575 bp 	DNA	SYN
DEFINITION  FH-TET3-pEF Sequencing Result
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..11575
                     /organism="FH-TET3-pEF Sequencing Result"
                     /mol_type="other DNA"
     promoter        359..1530
                     /label="EF1a_promoter"
     CDS             complement(650..1270)
                     /label="ORF frame 1"
     misc_feature    1478..1498
                     /label="EF1a_fwd_primer"
     promoter        1547..1565
                     /label="T7_promoter"
     CDS             1604..7066
                     /label="ORF frame 2"
     misc_feature    1607..1630
                     /label="FLAG"
     misc_feature    1643..1669
                     /label="HA"
     CDS             complement(4388..5602)
                     /label="ORF frame 1"
     CDS             complement(5974..6744)
                     /label="ORF frame 2"
     CDS             complement(6470..7153)
                     /label="ORF frame 1"
     misc_feature    complement(7176..7193)
                     /label="BGH_rev_primer"
     terminator      7179..7406
                     /label="bGH_PA_terminator"
     rep_origin      7469..7775
                     /label="f1_origin"
     misc_feature    complement(7889..7909)
                     /label="pBABE_3_primer"
     misc_feature    complement(7895..8110)
                     /label="SV40_enhancer"
     promoter        7907..8175
                     /label="SV40_promoter"
     rep_origin      8074..8151
                     /label="SV40_origin"
     misc_feature    8136..8155
                     /label="SV40pro_F_primer"
     CDS             8290..9084
                     /label="ORF frame 1"
     gene            8293..9081
                     /label="NeoR/KanR"
                     /gene="NeoR/KanR"
     terminator      9263..9382
                     /label="SV40_PA_terminator"
     misc_feature    9351..9370
                     /label="EBV_rev_primer"
     promoter        complement(9426..9444)
                     /label="M13_reverse_primer"
     misc_feature    complement(9443..9465)
                     /label="M13_pUC_rev_primer"
     promoter        complement(9479..9508)
                     /label="lac_promoter"
     gene            complement(10580..11440)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(10580..11440)
                     /label="ORF frame 1"
     promoter        complement(11482..11510)
                     /label="AmpR_promoter"
ORIGIN
    1 cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat ctGCTCTGAT
   61 GCCGCATAGT TAAGCCAGTA TCTGCTCCCT GCTTGTGTGT TGGAGGTCGC TGAGTAGTGC
  121 GCGAGCAAAA TTTAAGCTAC AACAAGGCAA GGCTTGACCG ACAATTGCAT GAAGAATCTG
  181 CTTAGGGTTA GGCGTTTTGC GCTGCTTCGC GATGTACGGG CCAGATATAC GCGTTGACAT
  241 TGATTATTGA CTAGGCTTTT GCAAAAAGCT TTGCAAAGAT GGATAAAGTT TTAAACAGAG
  301 AGGAATCTTT GCAGCTAATG GACCTTCTAG GTCTTGAAAG GAGTGGGAAT TGGCTCCGGT
  361 GCCCGTCAGT GGGCAGAGCG CACATCGCCC ACAGTCCCCG AGAAGTTGGG GGGAGGGGTC
  421 GGCAATTGAA CCGGTGCCTA GAGAAGGTGG CGCGGGGTAA ACTGGGAAAG TGATGTCGTG
  481 TACTGGCTCC GCCTTTTTCC CGAGGGTGGG GGAGAACCGT ATATAAGTGC AGTAGTCGCC
  541 GTGAACGTTC TTTTTCGCAA CGGGTTTGCC GCCAGAACAC AGGTAAGTGC CGTGTGTGGT
  601 TCCCGCGGGC CTGGCCTCTT TACGGGTTAT GGCCCTTGCG TGCCTTGAAT TACTTCCACC
  661 TGGCTGCAGT ACGTGATTCT TGATCCCGAG CTTCGGGTTG GAAGTGGGTG GGAGAGTTCG
  721 AGGCCTTGCG CTTAAGGAGC CCCTTCGCCT CGTGCTTGAG TTGAGGCCTG GCCTGGGCGC
  781 TGGGGCCGCC GCGTGCGAAT CTGGTGGCAC CTTCGCGCCT GTCTCGCTGC TTTCGATAAG
  841 TCTCTAGCCA TTTAAAATTT TTGATGACCT GCTGCGACGC TTTTTTTCTG GCAAGATAGT
  901 CTTGTAAATG CGGGCCAAGA TCTGCACACT GGTATTTCGG TTTTTGGGGC CGCGGGCGGC
  961 GACGGGGCCC GTGCGTCCCA GCGCACATGT TCGGCGAGGC GGGGCCTGCG AGCGCGGCCA
 1021 CCGAGAATCG GACGGGGGTA GTCTCAAGCT GGCCGGCCTG CTCTGGTGCC TGGCCTCGCG
 1081 CCGCCGTGTA TCGCCCCGCC CTGGGCGGCA AGGCTGGCCC GGTCGGCACC AGTTGCGTGA
 1141 GCGGAAAGAT GGCCGCTTCC CGGCCCTGCT GCAGGGAGCT CAAAATGGAG GACGCGGCGC
 1201 TCGGGAGAGC GGGCGGGTGA GTCACCCACA CAAAGGAAAA GGGCCTTTCC GTCCTCAGCC
 1261 GTCGCTTCAT GTGACTCCAC GGAGTACCGG GCGCCGTCCA GGCACCTCGA TTAGTTCTCG
 1321 AGCTTTTGGA GTACGTCGTC TTTAGGTTGG GGGGAGGGGT TTTATGCGAT GGAGTTTCCC
 1381 CACACTGAGT GGGTGGAGAC TGAAGTTAGG CCAGCTTGGC ACTTGATGTA ATTCTCCTTG
 1441 GAATTTGCCC TTTTTGAGTT TGGATCTTGG TTCATTCTCA AGCCTCAGAC AGTGGTTCAA
 1501 AGTTTTTTTC TTCCATTTCA GGTGTCGTGA GGAATTAGCT TGGTACTAAT ACGACTCACT
 1561 ATAGGGAGAC CCAAGCTGGC TAGGTAAGCT TGGTACCGCC ACCATGGACT ACAAGGACGA
 1621 CGATGACAAG CTCGATGGAG GATACCCCTA CGACGTGCCC GACTACGCCG GAGGACTCGA
 1681 CAGCCAGTTT CAGGTGCCCC TGGCCGTCCA GCCGGACCTG CCAGGCCTTT ATGACTTCCC
 1741 TCAGCGCCAG GTGATGGTAG GGAGCTTCCC GGGGTCTGGG CTCTCCATGG CTGGGAGTGA
 1801 GTCCCAACTC CGAGGGGGTG GAGATGGTCG AAAGAAACGG AAACGGTGTG GTACTTGTGA
 1861 GCCCTGCCGG CGGCTGGAAA ACTGTGGCGC TTGCACTAGC TGTACCAACC GCCGCACGCA
 1921 CCAGATCTGC AAACTGCGAA AATGTGAGGT GCTGAAGAAA AAAGTAGGGC TTCTCAAGGA
 1981 GGTGGAAATA AAGGCTGGTG AAGGAGCCGG GCCGTGGGGA CAAGGAGCGG CTGTCAAGAC
 2041 AGGCTCAGAG CTCAGCCCAG TTGATGGACC TGTTCCAGGT CAGATGGACT CAGGGCCAGT
 2101 GTACCATGGG GACTCACGGC AGCTAAGCGC CTCAGGGGTG CCGGTCAATG GTGCTAGAGA
 2161 GCCCGCTGGA CCCAGTCTGC TGGGGACTGG GGGTCCTTGG CGGGTAGACC AAAAGCCCGA
 2221 CTGGGAGGCT GCCCCAGGCC CAGCTCATAC TGCTCGCCTG GAAGATGCCC ACGATCTGGT
 2281 GGCCTTTTCG GCTGTGGCCG AAGCTGTGTC CTCTTATGGG GCCCTTAGCA CCCGGCTCTA
 2341 TGAAACCTTC AACCGTGAGA TGAGTCGTGA GGCTGGGAAC AACAGCAGGG GACCCCGGCC
 2401 AGGGCCTGAG GGCTGCTCTG CTGGCAGCGA AGACCTTGAC ACACTGCAGA CGGCCCTGGC
 2461 CCTCGCGCGG CATGGTATGA AACCACCCAA CTGCAACTGC GATGGCCCAG AATGCCCTGA
 2521 CTACCTCGAG TGGCTGGAGG GGAAGATCAA GTCTGTGGTC ATGGAAGGAG GGGAGGAGCG
 2581 GCCCAGGCTC CCAGGGCCTC TGCCTCCTGG TGAGGCCGGC CTCCCAGCAC CAAGCACCAG
 2641 GCCACTCCTC AGCTCAGAGG TGCCCCAGAT CTCTCCCCAA GAGGGCCTGC CCCTGTCCCA
 2701 GAGTGCCCTG AGCATTGCCA AGGAAAAAAA CATCAGCTTG CAGACCGCCA TTGCCATTGA
 2761 GGCCCTCACA CAGCTCTCCT CTGCCCTCCC GCAGCCTTCT CATTCCACCC CCCAGGCTTC
 2821 TTGCCCCCTT CCTGAGGCCT TGTCACCTCC TGCCCCTTTC AGATCTCCCC AGTCTTACCT
 2881 CCGGGCTCCC TCATGGCCTG TGGTTCCTCC TGAAGAGCAC TCATCTTTTG CTCCTGATAG
 2941 CTCTGCCTTC CCTCCAGCAA CTCCTAGAAC TGAGTTCCCT GAAGCCTGGG GCACTGACAC
 3001 CCCTCCAGCA ACGCCCCGGA GCTCCTGGCC CATGCCTCGC CCAAGCCCCG ATCCCATGGC
 3061 TGAACTGGAG CAGTTGTTGG GCAGCGCCAG TGATTACATC CAGTCAGTAT TCAAGCGGCC
 3121 TGAGGCCCTG CCTACCAAGC CCAAGGTCAA GGTGGAGGCA CCCTCTTCCT CCCCGGCCCC
 3181 GGCCCCATCC CCTGTACTTC AGAGGGAGGC TCCCACGCCA TCCTCGGAGC CCGACACCCA
 3241 CCAGAAGGCC CAGACCGCCC TGCAGCAGCA CCTCCACCAC AAGCGCAGCC TCTTCCTAGA
 3301 ACAGGTGCAC GACACCTCCT TCCCTGCTCC TTCAGAGCCT TCTGCTCCTG GCTGGTGGCC
 3361 CCCACCAAGT TCACCTGTCC CACGGCTTCC AGACAGACCA CCCAAGGAGA AGAAGAAGAA
 3421 GCTCCCAACA CCAGCTGGAG GTCCCGTGGG AACGGAGAAA GCTGCCCCTG GGATCAAGCC
 3481 CAGTGTCCGA AAGCCCATTC AGATCAAGAA GTCCAGGCCC CGGGAAGCAC AGCCCCTCTT
 3541 CCCACCTGTC CGACAGATTG TCCTGGAAGG GCTTAGGTCC CCAGCCTCCC AGGAAGTGCA
 3601 GGCTCATCCA CCGGCCCCTC TGCCTGCCTC ACAGGGCTCT GCTGTGCCCC TGCCCCCAGA
 3661 ACCTTCTCTT GCGCTATTTG CACCTAGTCC CTCCAGGGAC AGCCTGCTGC CCCCTACTCA
 3721 GGAAATGAGG TCCCCCAGCC CCATGACAGC CTTGCAGCCA GGCTCCACTG GCCCTCTTCC
 3781 CCCTGCCGAT GACAAGCTGG AAGAGCTCAT CCGGCAGTTT GAGGCTGAAT TTGGAGATAG
 3841 CTTTGGGCTT CCCGGCCCCC CTTCTGTGCC CATTCAGGAC CCCGAGAACC AGCAAACATG
 3901 TCTCCCAGCC CCTGAGAGCC CCTTTGCTAC CCGTTCCCCC AAGCAAATCA AGATTGAGTC
 3961 TTCGGGGGCT GTGACTGTGC TCTCAACCAC CTGCTTCCAT TCAGAGGAGG GAGGACAGGA
 4021 GGCCACACCC ACCAAGGCTG AGAACCCACT CACACCCACC CTCAGTGGCT TCTTGGAGTC
 4081 ACCTCTTAAG TACCTGGACA CACCCACCAA GAGTCTGCTG GACACACCTG CCAAGAGAGC
 4141 CCAGGCCGAG TTCCCCACCT GCGATTGCGT CGAACAAATA GTGGAGAAAG ATGAAGGTCC
 4201 ATATTATACT CACTTGGGAT CTGGCCCCAC GGTCGCCTCT ATCCGGGAAC TCATGGAGGA
 4261 GCGGTATGGA GAGAAGGGGA AAGCCATCCG GATCGAGAAG GTCATCTACA CGGGGAAGGA
 4321 GGGAAAGAGC TCCCGCGGTT GCCCCATTGC AAAGTGGGTG ATCCGCAGGC ACACGCTGGA
 4381 GGAGAAGCTA CTCTGCCTGG TGCGGCACCG GGCAGGCCAC CACTGCCAGA ACGCTGTGAT
 4441 CGTCATCCTC ATCCTGGCCT GGGAGGGCAT TCCCCGTAGC CTCGGAGACA CCCTCTACCA
 4501 GGAGCTCACC GACACCCTCC GGAAGTATGG GAACCCCACC AGCCGGAGAT GCGGCCTCAA
 4561 CGATGACCGG ACCTGCGCTT GCCAAGGCAA AGACCCCAAC ACCTGTGGTG CCTCCTTCTC
 4621 CTTTGGTTGT TCCTGGAGCA TGTACTTCAA CGGCTGCAAG TATGCTCGGA GCAAGACACC
 4681 TCGCAAGTTC CGCCTCGCAG GGGACAATCC CAAAGAGGAA GAAGTGCTCC GGAAGAGTTT
 4741 CCAGGACCTG GCCACCGAAG TCGCTCCCCT GTACAAGCGA CTGGCCCCTC AGGCCTATCA
 4801 GAACCAGGTG ACCAACGAGG AAATAGCGAT TGACTGCCGT CTGGGGCTGA AGGAAGGACG
 4861 GCCCTTCGCG GGGGTCACGG CCTGCATGGA CTTCTGTGCC CACGCCCACA AGGACCAGCA
 4921 TAACCTCTAC AATGGGTGCA CCGTGGTCTG CACCCTGACC AAGGAAGACA ATCGCTGCGT
 4981 GGGCAAGATT CCCGAGGATG AGCAGCTGCA TGTTCTCCCC CTGTACAAGA TGGCCAACAC
 5041 GGATGAGTTT GGTAGCGAGG AGAACCAGAA TGCAAAGGTG GGCAGCGGAG CCATCCAGGT
 5101 GCTCACCGCC TTCCCCCGCG AGGTCCGACG CCTGCCCGAG CCTGCCAAGT CCTGCCGCCA
 5161 GCGGCAGCTG GAAGCCAGAA AGGCAGCAGC CGAGAAGAAG AAGATTCAGA AGGAGAAGCT
 5221 GAGCACTCCG GAGAAGATCA AGCAGGAGGC CCTGGAGCTG GCGGGCATTA CGTCGGACCC
 5281 AGGCCTGTCT CTGAAGGGTG GATTGTCCCA GCAAGGCCTG AAGCCCTCCC TCAAGGTGGA
 5341 GCCGCAGAAC CACTTCAGCT CCTTCAAGTA CAGCGGCAAC GCGGTGGTGG AGAGCTACTC
 5401 GGTGCTGGGC AACTGCCGGC CCTCCGACCC TTACAGCATG AACAGCGTGT ACTCCTACCA
 5461 CTCCTACTAT GCACAGCCCA GCCTGACCTC CGTCAATGGC TTCCACTCCA AGTACGCTCT
 5521 CCCGTCTTTT AGCTACTATG GCTTTCCATC CAGCAACCCC GTCTTCCCCT CTCAGTTCCT
 5581 GGGTCCTGGT GCCTGGGGGC ATAGTGGCAG CAGTGGCAGT TTTGAGAAGA AGCCAGACCT
 5641 CCACGCTCTG CACAACAGCC TGAGCCCGGC CTACGGTGGT GCTGAGTTTG CCGAGCTGCC
 5701 CAGCCAGGCT GTTCCCACAG ACGCCCACCA CCCCACTCCT CACCACCAGC AGCCTGCGTA
 5761 CCCAGGCCCC AAGGAGTATC TGCTTCCCAA GGCCCCCCTA CTCCACTCAG TGTCCAGGGA
 5821 CCCCTCCCCC TTTGCCCAGA GCTCCAACTG CTACAACAGA TCCATCAAGC AAGAGCCAGT
 5881 AGACCCGCTG ACCCAGGCTG AGCCTGTGCC CAGAGACGCT GGCAAGATGG GCAAGACACC
 5941 TCTGTCCGAG GTGTCTCAGA ATGGAGGACC CAGTCACCTT TGGGGACAGT ACTCAGGAGG
 6001 CCCAAGCATG TCCCCCAAGA GGACTAACGG TGTGGGTGGC AGCTGGGGTG TGTTCTCGTC
 6061 TGGGGAGAGT CCTGCCATCG TCCCTGACAA GCTCAGTTCC TTTGGGGCCA GCTGCCTGGC
 6121 CCCTTCCCAC TTCACAGATG GCCAGTGGGG GCTGTTCCCC GGTGAGGGGC AGCAGGCAGC
 6181 TTCCCACTCT GGAGGACGGC TGCGAGGCAA ACCGTGGAGC CCCTGCAAGT TTGGGAACAG
 6241 CACCTCGGCC TTGGCTGGGC CCAGCCTGAC TGAGAAGCCG TGGGCGCTGG GGGCAGGGGA
 6301 TTTCAACTCG GCCCTGAAAG GTAGTCCTGG GTTCCAAGAC AAGCTGTGGA ACCCCATGAA
 6361 AGGAGAGGAG GGCAGGATTC CAGCCGCAGG GGCCAGCCAG CTGGACAGGG CCTGGCAGTC
 6421 CTTTGGTCTG CCCCTGGGAT CCAGCGAGAA GCTGTTTGGG GCTCTGAAGT CAGAGGAGAA
 6481 GCTGTGGGAC CCCTTCAGCC TGGAGGAGGG GCCGGCTGAG GAGCCCCCCA GCAAGGGAGC
 6541 GGTGAAGGAG GAGAAGGGCG GTGGTGGTGC GGAGGAGGAA GAGGAGGAGC TGTGGTCGGA
 6601 CAGTGAACAC AACTTCCTGG ACGAGAACAT CGGCGGCGTG GCCGTGGCCC CAGCCCACGG
 6661 CTCCATCCTC ATCGAGTGTG CCCGGCGGGA GCTGCACGCC ACCACGCCGC TTAAGAAGCC
 6721 CAACCGCTGC CACCCCACCC GCATCTCGCT GGTCTTCTAC CAGCACAAGA ACCTCAACCA
 6781 GCCCAACCAC GGGCTGGCCC TCTGGGAAGC CAAGATGAAG CAGCTGGCGG AGAGGGCACG
 6841 GGCACGGCAG GAGGAGGCTG CCCGGCTGGG CCTGGGCCAG CAGGAGGCCA AGCTCTACGG
 6901 GAAGAAGCGC AAGTGGGGGG GCACTGTGGT TGCTGAGCCC CAGCAGAAAG AGAAGAAGGG
 6961 GGTCGTCCCC ACCCGGCAGG CACTGGCTGT GCCCACAGAC TCGGCGGTCA CCGTGTCCTC
 7021 CTATGCCTAC ACGAAGGTCA CTGGCCCCTA CAGCCGCTGG ATCTAGTCTA GAGGGCCCTT
 7081 CGAAGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGCGTA CCGGTCATCA
 7141 TCACCATCAC CATTGAGTTT AAACCCGCTG ATCAGCCTCG ACTGTGCCTT CTAGTTGCCA
 7201 GCCATCTGTT GTTTGCCCCT CCCCCGTGCC TTCCTTGACC CTGGAAGGTG CCACTCCCAC
 7261 TGTCCTTTCC TAATAAAATG AGGAAATTGC ATCGCATTGT CTGAGTAGGT GTCATTCTAT
 7321 TCTGGGGGGT GGGGTGGGGC AGGACAGCAA GGGGGAGGAT TGGGAAGACA ATAGCAGGCA
 7381 TGCTGGGGAT GCGGTGGGCT CTATGGCTTC TGAGGCGGAA AGAACCAGCT GGGGCTCTAG
 7441 GGGGTATCCC CACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG
 7501 CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC
 7561 CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG
 7621 GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC
 7681 ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT
 7741 CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC
 7801 TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA
 7861 ACAAAAATTT AACGCGAATT AATTCTGTGG AATGTGTGTC AGTTAGGGTG TGGAAAGTCC
 7921 CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCAGG
 7981 TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG
 8041 TCAGCAACCA TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC
 8101 GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC
 8161 TCTGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC
 8221 AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAAGAGAC AGGATGAGGA
 8281 TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG
 8341 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC
 8401 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG
 8461 AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC
 8521 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG
 8581 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT
 8641 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG
 8701 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT
 8761 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGCGC
 8821 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG
 8881 GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGACCGC
 8941 TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT
 9001 GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT
 9061 CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGCGAAATGA CCGACCAAGC
 9121 GACGCCCAAC CTGCCATCAC GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG
 9181 CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC CAGCGCGGGG ATCTCATGCT
 9241 GGAGTTCTTC GCCCACCCCA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA
 9301 TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC
 9361 CAAACTCATC AATGTATCTT ATCATGTCTG TATACCGTCG ACCTCTAGCT AGAGCTTGGC
 9421 GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA
 9481 CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC
 9541 ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA
 9601 TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC
 9661 CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC
 9721 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC
 9781 AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC
 9841 CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT
 9901 AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC
 9961 CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT
10021 CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG
10081 AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC
10141 CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA
10201 GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA
10261 GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA
10321 GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC
10381 AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG
10441 ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA
10501 TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG
10561 AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT
10621 GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG
10681 AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC
10741 CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA
10801 CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC
10861 CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT
10921 CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC
10981 CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT
11041 TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC
11101 CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT
11161 GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA
11221 GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA
11281 TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG
11341 CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA
11401 AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT
11461 ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA
11521 AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGT

//

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