| 出品公司: | Promega |
|---|---|
| 载体名称: | pGEM-7Zf(+) |
| 质粒类型: | 大肠杆菌克隆载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 启动子: | -- |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 2997 bp |
| 5' 测序引物及序列: | M13 fwd |
| 3' 测序引物及序列: | M13 rev |
| 载体标签: | -- |
| 载体抗性: | Ampicillin |
| 筛选标记: | -- |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型: | -- |
| 病毒/非病毒: | 非病毒 |
买家导航
pGEM-7Zf(+)载体 pGEM7Zf(+)质粒基本信息
pGEM-7Zf(+)质粒图谱 pGEM7Zf(+)载体图谱和pGEM-7Zf(+)载体序列 pGEM7Zf(+)质粒序列多克隆位点信息
载体图谱.jpg)
多克隆位点.jpg "pEGM-7ZF(+)载体多克隆位点 点击看大图")
载体特征.jpg)
pGEM-7Zf(+)质粒 pGEM7Zf(+)载体简介
The pGEM?-7Zf(+) Vector(a) is a derivative of the pGEM?-3Zf(+) Vector(a) and contains
the origin of replication of the filamentous phage f1. The plasmid serves as a
standard cloning vector, as a template for in vitro transcription, and it can be used
for the production of circular ssDNA. The plasmid contains SP6 and T7 RNA polymerase
promoters flanking a region of multiple cloning sites within the α-peptide
coding region of β-galactosidase (1). Insertional inactivation of the α-peptide allows
recombinant clones to be directly identified by color screening on indicator plates.
The multiple cloning region is unique and includes restriction sites for Apa I, Aat II,
Sph I, Xba I, Xho I, EcoR I, Kpn I, Sma I, Csp45 I, Cla I, Hind III, BamH I, Sac I,
BstX I and Nsi I. This arrangement is designed specifically for generation of unidirectional
deletions with the Erase-a-Base? System(b). The polylinker contains
restriction enzyme sites that produce 5′ overhangs or blunt ends (sensitive to
Exonuclease III) flanked on both sides by blocks of restriction sites that generate
3′ overhangs (resistant to Exonuclease III).
For induction of ssDNA, bacterial cells containing pGEM?-7Zf(+) recombinants are
infected with an appropriate helper phage. The plasmid then enters the f1 replication
mode, and the resulting ssDNA is exported from the cell as an encapsidated viruslike
particle. The sequence of the ssDNA rescued upon infection with helper phage
is complementary to the sequence shown in Figure 1. The exported ssDNA can be
used for mutagenesis in vitro or can be sequenced using the T7 Promoter Primer
(Cat.# Q5021) or pUC/M13 Forward Primer (Cat.# Q5391, Q5601).
pGEM-7Zf(+)质粒序列 pGEM7Zf(+)载体序列
LOCUS pGEM-7Zf- 3000 bp DNA SYN
DEFINITION pGEM-7Zf-
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..3000
/organism="pGEM-7Zf-"
/mol_type="other DNA"
misc_feature complement(123..140)
/label="Sp6_primer"
promoter complement(154..172)
/label="M13_reverse_primer"
misc_feature complement(171..193)
/label="M13_pUC_rev_primer"
promoter complement(207..236)
/label="lac_promoter"
rep_origin complement(545..1164)
/label="pBR322_origin"
gene complement(1319..2179)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(1319..2179)
/label="ORF frame 3"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCHTLLSRIDAGQEQLGRRARYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
promoter complement(2221..2249)
/label="AmpR_promoter"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCHTLLSRIDAGQEQLGRRARYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
rep_origin 2382..2688
/label="f1_origin"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCHTLLSRIDAGQEQLGRRARYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
misc_feature complement(2832..2980)
/label="lacZ_a"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCHTLLSRIDAGQEQLGRRARYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
misc_feature 2946..2968
/label="M13_pUC_fwd_primer"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCHTLLSRIDAGQEQLGRRARYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
promoter 2961..2977
/label="M13_forward20_primer"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCHTLLSRIDAGQEQLGRRARYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
promoter 2984..2
/label="T7_promoter"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCHTLLSRIDAGQEQLGRRARYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 gggcgaattg ggcccgacgt cgcatgctcc tctagactcg aggaattcgg taccccgggt
61 tcgaaatcga taagcttgga tccggagagc tcccaacgcg ttggatgcat agcttgagta
121 ttctatagtg tcacctaaat agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
181 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
241 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
301 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
361 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
421 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
481 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
541 aggccgcgtt gctggcgttt ttcgataggc tccgcccccc tgacgagcat cacaaaaatc
601 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
661 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
721 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
781 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
841 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
901 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
961 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
1021 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
1081 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
1141 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
1201 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
1261 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
1321 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
1381 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
1441 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
1501 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
1561 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
1621 ttgttggcat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
1681 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
1741 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
1801 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
1861 tgactggtga gtactcaacc aagtcattct gagaataccg cgcccggcga ccgagttgct
1921 cttgcccggc gtcaatacgg gataatagtg tatgacatag cagaacttta aaagtgctca
1981 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
2041 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
2101 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
2161 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
2221 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
2281 cgcgcacatt tccccgaaaa gtgccacctg tatgcggtgt gaaataccgc acagatgcgt
2341 aaggagaaaa taccgcatca ggcgacgcgc cctgtagcgg cgcattaagc gcggcgggtg
2401 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
2461 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
2521 ggctcccttt agggttccga tttagagctt tacggcacct cgaccgcaaa aaacttgatt
2581 tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
2641 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
2701 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
2761 atgagctgat ttaacaaata tttaacgcga attttaacaa aatattaacg tttacaattt
2821 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
2881 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
2941 gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttgtaatacg actcactata
//
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pGEM-7Zf(+)质粒 pGEM7Zf(+)载体应用举例
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