| 出品公司: | Invitrogen |
|---|---|
| 载体名称: | pFastBacDual, pFastBac Dual |
| 质粒类型: | 昆虫细胞表达载体 |
| 启动子: | Polyhedrin, P10 |
| 表达水平: | 高 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 5238 bp |
| 5' 测序引物: | pFastbac-F: TATTCCGGATTATTCATACC |
| 3' 测序引物: | pFastBac-R: ACAAATGTGGTATGGCTGA |
| 载体标签: | N-His, N-TEV |
| 载体抗性: | 氨苄 |
| 筛选标记: | 庆大霉素 |
| 备注: | 在昆虫细胞中，能够同时表达两个基因。 |
| 产品目录号: | 10584-027 |
| 稳定性: | 稳表达 Stable |
| 组成型: | 组成型 Constitutive |
| 病毒/非病毒: | 杆状病毒 |
买家导航
pFastBac Dual载体 pFastBacDual质粒基本信息
pFastBac Dual质粒图谱 pFastBacDual载体图谱和pFastBac Dual载体序列 pFastBacDual质粒序列多克隆位点信息
pFastBac Dual质粒 pFastBacDual载体简介
pFastBac Dual昆虫细胞表达载体包含有两个启动子，能够同时表达两个外源基因，一个表达基因是polyhedrin (PH)启动子控制，另一个外源基因是由p10启动子控制。
pFastBac Dual质粒序列 pFastBacDual载体序列
LOCUS pFastBac\Dual 5238 bp DNA circular SYN 16-MAY-2012
DEFINITION pFastBac Dual.
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
source 1..5238
/organism="pFastBac Dual"
/mol_type="other DNA"
/vntifkey="98"
rep_origin 102..557
/vntifkey="33"
/label=f1_origin
promoter 619..647
/vntifkey="30"
/label=AmpR_promoter
CDS 689..1549
/gene="Ampicillin"
/vntifkey="4"
/label=Ampicillin
/note="ORF frame 2"
gene 689..1549
/gene="Ampicillin"
/vntifkey="60"
rep_origin 1704..2323
/vntifkey="33"
/label=pUC_origin
gene complement(2902..3435)
/gene="gent"
/vntifkey="60"
terminator complement(3996..4266)
/vntifkey="43"
/label=TK_PA_terminator
misc_feature 4494..4511
/vntifkey="21"
/label=polyhedrin_fwd_primer
promoter 4478..4606
/vntifkey="30"
/label=PH_promoter
terminator 4831..4962
/vntifkey="43"
/label=SV40_PA_terminator
misc_feature 4919..4938
/vntifkey="21"
/label=EBV_rev_primer
misc_feature 2611..2835
/vntifkey="21"
/label=Tn7R
CDS complement(2902..3435)
/vntifkey="4"
/label=Gentimicin\Resistance\Gene
misc_feature complement(4274..4337)
/vntifkey="21"
/label=MCS
misc_feature 4606..4704
/vntifkey="21"
/label=MCS
promoter complement(4338..4459)
/vntifkey="30"
/label=P10\Promoter
misc_feature complement(4991..5156)
/vntifkey="21"
/label=Tn7L
misc_signal complement(3992..4274)
/vntifkey="87"
/label=HSV\TK\polyadenylation\signal
misc_signal 4722..4962
/vntifkey="87"
/label=SV40\polyadenylation\signal
BASE COUNT 1294 a 1282 c 1259 g 1403 t
ORIGIN
1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
61 ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
121 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
181 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
241 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
301 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
361 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
421 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
481 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
541 attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
601 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
661 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
721 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
781 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
841 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
901 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
961 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
1021 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
1081 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
1141 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
1201 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
1261 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
1321 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
1381 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
1441 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
1501 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
1561 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
1621 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
1681 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
1741 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
1801 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
1861 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
1921 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
1981 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
2041 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
2101 acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
2161 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
2221 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
2281 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
2341 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
2401 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
2461 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
2521 tctgtgcggt atttcacacc gcagaccagc cgcgtaacct ggcaaaatcg gttacggttg
2581 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
2641 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
2701 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
2761 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
2821 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc
2881 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac
2941 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc
3001 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga gattgatgag
3061 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat
3121 ctcactacgc ggctgctcaa acctgggcag aacgtaagcc gcgagagcgc caacaaccgc
3181 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta
3241 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag
3301 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat
3361 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt
3421 gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
3481 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga
3541 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
3601 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc
3661 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg
3721 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg
3781 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg ccctggcttc
3841 aggagatcgg tagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag
3901 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct
3961 atagttctag tggttggcct acgtacccgt agtggctatg gcagggcttg ccgccccgac
4021 gttggctgcg agccctgggc cttcacccga acttgggggt tggggtgggg aaaaggaaga
4081 aacgcgggcg tattggtccc aatggggtct cggtggggta tcgacagagt gccagccctg
4141 ggaccgaacc ccgcgtttat gaacaaacga cccaacaccc gtgcgtttta ttctgtcttt
4201 ttattgccgt catagcgcgg gttccttccg gtattgtctc cttccgtgtt tcagttagcc
4261 tcccccatct cccggtaccg catgctatgc atcagctgct agcaccatgg ctcgagatcc
4321 cgggtgatca agtcttcgtc gagtgattgt aaataaaatg taatttacag tatagtattt
4381 taattaatat acaaatgatt tgataataat tcttatttaa ctataatata ttgtgttggg
4441 ttgaattaaa ggtccgtata ctccggaata ttaatagatc atggagataa ttaaaatgat
4501 aaccatctcg caaataaata agtattttac tgttttcgta acagttttgt aataaaaaaa
4561 cctataaata ttccggatta ttcataccgt cccaccatcg ggcgcggatc ccggtccgaa
4621 gcgcgcggaa ttcaaaggcc tacgtcgacg agctcactag tcgcggccgc tttcgaatct
4681 agagcctgca gtctcgacaa gcttgtcgag aagtactaga ggatcataat cagccatacc
4741 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
4801 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
4861 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
4921 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tctgatcact gcttgagcct
4981 aggagatccg aaccagataa gtgaaatcta gttccaaact attttgtcat ttttaatttt
5041 cgtattagct tacgacgcta cacccagttc ccatctattt tgtcactctt ccctaaataa
5101 tccttaaaaa ctccatttcc acccctccca gttcccaact attttgtccg cccacagcgg
5161 ggcatttttc ttcctgttat gtttttaatc aaacatcctg ccaactccat gtgacaaacc
5221 gtcatcttcg gctacttt
//
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