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LOCUS Exported 2916 bp ds-DNA circular SYN 13-AUG-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pENTR-L1pLaclacZaL4
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 2916)
AUTHORS Biofeng Lab.
TITLE Direct Submission
JOURNAL Exported Saturday, August 13, 2016 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..2916
/organism="synthetic DNA construct"
/mol_type="other DNA"
terminator 268..295
/note="rrnB T2 terminator"
/note="transcription terminator T2 from the E. coli rrnB
gene"
terminator 387..473
/gene="Escherichia coli rrnB"
/note="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
primer_bind 537..553
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 569..668
/gene="mutant version of attL"
/bound_moiety="LR Clonase(TM)"
/note="attL1"
/note="recombination site for the Gateway(R) LR reaction"
promoter 694..724
/note="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 732..748
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 756..772
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
gene 768..983
/note="lacZ-alpha"
misc_feature 785..841
/note="MCS"
/note="pUC18/19 multiple cloning site"
primer_bind complement(842..858)
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(1031..1126)
/gene="mutant version of attL"
/bound_moiety="LR Clonase(TM)"
/note="attL4"
/note="recombination site for the Gateway(R) LR reaction"
promoter complement(1158..1176)
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(1181..1197)
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
CDS 1310..2119
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/note="KanR"
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
rep_origin 2266..2854
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
661 agcaggctag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
721 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
781 cgccaagctt gcatgcctgc aggtcgactc tagaggatcc ccgggtaccg agctcgaatt
841 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
901 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
961 gcccttccca acagttgcgc agctaacact ataaaaagct gatgcagacg gggctttttt
1021 atgcaggacc caacttttct atacaaagtt ggcattataa gaaagcattg cttatcaatt
1081 tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgca
1141 gggcggccgc gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca
1201 gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc
1261 atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat
1321 tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta
1381 taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa
1441 gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac
1501 agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca
1561 ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc
1621 attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt
1681 gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt
1741 atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt
1801 tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt
1861 gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt
1921 tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata
1981 ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg
2041 gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat
2101 gctcgatgag tttttctaat cagaattggt taattggttg taacactggc agagcattac
2161 gctgacttga cgggacggcg caagctcatg accaaaatcc cttaacgtga gttacgcgtc
2221 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
2281 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
2341 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
2401 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
2461 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
2521 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
2581 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
2641 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
2701 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
2761 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
2821 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
2881 gttcctggcc ttttgctggc cttttgctca catgtt
//
