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pENTR-L1-pLac-lacZa-R5载体质粒基本信息
pENTR-L1-pLac-lacZa-R5质粒图谱载体图谱和pENTR-L1-pLac-lacZa-R5载体序列质粒序列多克隆位点信息
pENTR-L1-pLac-lacZa-R5质粒载体简介
pENTR-L1-pLac-lacZa-R5质粒序列载体序列
LOCUS Exported 2968 bp ds-DNA circular SYN 13-AUG-2016 DEFINITION synthetic circular DNA KEYWORDS pENTR-L1-pLac-lacZa-R5 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2968) AUTHORS Biofeng Lab. TITLE Direct Submission FEATURES Location/Qualifiers source 1..2968 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 268..295 /note="rrnB T2 terminator" /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /note="rrnB T1 terminator" /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 537..553 /note="M13 fwd" /note="common sequencing primer, one of multiple similar variants" protein_bind 569..668 /gene="mutant version of attL" /bound_moiety="LR Clonase(TM)" /note="attL1" /note="recombination site for the Gateway(R) LR reaction" promoter 694..724 /note="lac promoter" /note="promoter for the E. coli lac operon" protein_bind 732..748 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 756..772 /note="M13 rev" /note="common sequencing primer, one of multiple similar variants" gene 767..983 /note="lacZ-alpha" misc_feature 785..841 /note="MCS" /note="pUC18/19 multiple cloning site" primer_bind complement(842..858) /note="M13 fwd" /note="common sequencing primer, one of multiple similar variants" protein_bind 1031..1154 /gene="mutant version of attR" /bound_moiety="LR Clonase(TM)" /note="attR5" /note="recombination site for the Gateway(R) LR reaction" promoter complement(1210..1228) /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1233..1249) /note="M13 rev" /note="common sequencing primer, one of multiple similar variants" CDS 1362..2171 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /note="KanR" /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" rep_origin 2318..2906 /direction=RIGHT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 661 agcaggctag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat 721 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta 781 cgccaagctt gcatgcctgc aggtcgactc tagaggatcc ccgggtaccg agctcgaatt 841 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc 901 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc 961 gcccttccca acagttgcgc agctaacact ataaaaaggc agtgatgcag acggggcttt 1021 tttatgcagg caactttgta tacaaaagtt gaacgagaaa cgtaaaatga tataaatatc 1081 aatatattaa attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat 1141 atccagtcac tatgaatcaa ctacttagat ggtattagtg acctgtactg cagggcggcc 1201 gcgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc 1261 ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa 1321 taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg 1381 aaacgtcgag gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg 1441 ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagcccgatg 1501 cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga 1561 tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc 1621 gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca gcattccagg 1681 tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc 1741 gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc 1801 tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg 1861 agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct 1921 caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg 1981 ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc 2041 ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc 2101 aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg 2161 agtttttcta atcagaattg gttaattggt tgtaacactg gcagagcatt acgctgactt 2221 gacgggacgg cgcaagctca tgaccaaaat cccttaacgt gagttacgcg tcgttccact 2281 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg 2341 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc 2401 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata 2461 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta 2521 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc 2581 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg 2641 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac 2701 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg 2761 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt 2821 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct 2881 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg 2941 ccttttgctg gccttttgct cacatgtt //