³öÆ·¹«Ë¾: | Invitrogen |
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ÔØÌåÃû³Æ: | pENTR-GeneBLAzer |
ÖÊÁ£ÀàÐÍ: | GatewayÔØÌ壻ÈëÃÅÔØÌå |
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Æô¶¯×Ó: | ÎÞ |
¿Ë¡·½·¨: | ÏÞÖÆÐÔÄÚÇÐø£»¶à¿Ë¡λµã |
ÔØÌå´óС: | 3339 bp |
5' ²âÐòÒýÎï¼°ÐòÁÐ: | pENTR-F: CTACAAACTCTTCCTGTTAGTTAG |
3' ²âÐòÒýÎï¼°ÐòÁÐ: | pENTR-R: ATGGCTCATAACACCCCTTG |
ÔØÌå±êÇ©: | ÎÞ |
ÔØÌ忹ÐÔ: | ¿¨ÄÇÃ¹ËØ |
ÕæºËɸѡ±ê¼Ç: | ÎÞ |
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pENTR-GeneBLAzer
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LOCUS Exported 3339 bp ds-DNA circular SYN 13-AUG-2016 DEFINITION synthetic circular DNA KEYWORDS pENTR-GeneBLAzer SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3339) AUTHORS Biofeng Lab. FEATURES Location/Qualifiers source 1..3339 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 268..295 /note="rrnB T2 terminator" /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /note="rrnB T1 terminator" /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 537..553 /note="M13 fwd" /note="common sequencing primer, one of multiple similar variants" protein_bind 569..668 /gene="mutant version of attL" /bound_moiety="LR Clonase(TM)" /note="attL1" /note="recombination site for the Gateway(R) LR reaction" CDS 671..1465 /codon_start=1 /gene="bla(M)" /product="beta-lactamase lacking the signal sequence" /note="bla(M)" /note="allows cytosolic expression of beta-lactamase" /translation="MDPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMM STFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAIT MSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMA TTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGII AALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW" protein_bind complement(1464..1563) /gene="mutant version of attL" /bound_moiety="LR Clonase(TM)" /note="attL2" /note="recombination site for the Gateway(R) LR reaction" promoter complement(1581..1599) /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1604..1620) /note="M13 rev" /note="common sequencing primer, one of multiple similar variants" CDS 1733..2542 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /note="KanR" /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" rep_origin 2689..3277 /direction=RIGHT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 661 agcaggcacc atggacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg 721 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg 781 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt 841 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga 901 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga 961 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac 1021 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg 1081 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac 1141 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct 1201 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct 1261 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg 1321 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat 1381 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg 1441 tgcctcactg attaagcatt ggtagacagc tttcttgtac aaagttggca ttataagaaa 1501 gcattgctta tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat 1561 ttgccatcca gctgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg 1621 gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc 1681 atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca 1741 tattcaacgg gaaacgtcga ggccgcgatt aaattccaac atggatgctg atttatatgg 1801 gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc gcttgtatgg 1861 gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt 1921 tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa 1981 gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc ccggaaaaac 2041 agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc 2101 agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg 2161 cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga 2221 ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact 2281 tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat 2341 ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg 2401 ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa 2461 acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt 2521 gatgctcgat gagtttttct aatcagaatt ggttaattgg ttgtaacact ggcagagcat 2581 tacgctgact tgacgggacg gcgcaagctc atgaccaaaa tcccttaacg tgagttacgc 2641 gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt 2701 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg 2761 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca 2821 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt 2881 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga 2941 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc 3001 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact 3061 gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga gaaaggcgga 3121 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg 3181 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt 3241 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt 3301 acggttcctg gccttttgct ggccttttgc tcacatgtt //
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