| 质粒名称: | pBiFC-VN155(I152L) |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 27097 |
| 存储实验室: | Chang-Deng Hu |
|
载体骨架基本信息 |
|
| 载体名称: | pMyc-CMV |
| 载体出品公司: | Clontech |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 3791 bp |
| 载体类型: | 哺乳细胞表达 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | VN155(I152L) |
| 物种来源: | 维多利亚水母 |
| 插入大小: | 462 bp |
| 基因突变: | I152L |
| 标签/融合蛋白: | Myc（N端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | KpnI(未破坏) |
| 3' 克隆位点: | NotI (未破坏) |
| 5' 测序引物 | n/a |
| 3' 测序引物 | n/a |
|
文献引用方法 |
|
| 材料和方法部分: | pBiFC-VN155(I152L) was a gift from Chang-Deng Hu (Addgene plasmid # 27097) |
| 参考文献部分: | An improved bimolecular fluorescence complementation assay with a high signal-to-noise ratio. Kodama Y, Hu CD. Biotechniques. 2010 Nov . 49(5):793-805. 10.2144/000113519 PubMed 21091444 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
质粒图谱.png)
载体简介
载体序列
LOCUS pBiFC-VN155(I152L) 4289 bp DNA SYN
DEFINITION pBiFC-VN155(I152L)
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..4289
/organism="pBiFC-VN155(I152L)"
/mol_type="other DNA"
CDS 1..588
/label="ORF frame 1"
misc_feature 4..27
/label="Myc_primer"
gene 124..567
/label="EGFP (0 - 443)"
/gene="EGFP (0 - 443)"
misc_feature complement(166..187)
/label="EGFP_N_primer"
terminator complement(610..729)
/label="SV40_PA_terminator"
misc_feature complement(624..643)
/label="EBV_rev_primer"
promoter complement(841..859)
/label="M13_reverse_primer"
misc_feature complement(858..880)
/label="M13_pUC_rev_primer"
promoter complement(894..923)
/label="lac_promoter"
gene complement(2006..2866)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(2006..2866)
/label="ORF frame 2"
promoter complement(2908..2936)
/label="AmpR_promoter"
misc_feature complement(3095..3117)
/label="pGEX_3_primer"
misc_feature complement(3316..3459)
/label="lacZ_a"
misc_feature 3430..3452
/label="M13_pUC_fwd_primer"
promoter 3445..3461
/label="M13_forward20_primer"
misc_feature 3492..3779
/label="CAG_enhancer"
promoter 3497..3989
/label="CMV_immearly_promoter"
misc_feature 3946..3966
/label="CMV_fwd_primer"
promoter 3947..4016
/label="CMV_promoter"
promoter 3959..4078
/label="CMV2_promoter"
misc_feature 3990..4009
/label="pCEP_fwd_primer"
misc_feature 3992..4016
/label="LNCX_primer"
misc_feature 4092..4118
/label="pCMV_primer"
misc_feature 4133..4229
/label="modSV40_late_16s_int"
misc_feature 4133..4163
/label="SV40_late_19s_int"
CDS 4211..659
/label="ORF frame 2"
ORIGIN
1 ATGGCATCAA TGCAGAAGCT GATCTCAGAG GAGGACCTGC TTATGGCCAT GGAGGCCCGA
61 ATTCGGTCGA CCGAGATCTC TCGAGGTACC GGAGGTGGCG GGAGCGGAGG TGGCGGGAGT
121 ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC
181 GGCGACGTAA ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC
241 GGCAAGCTGA CCCTGAAGCT GATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC
301 CTCGTGACCA CCCTGGGCTA CGGCCTGCAG TGCTTCGCCC GCTACCCCGA CCACATGAAG
361 CAGCACGACT TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC
421 TTCAAGGACG ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG
481 GTGAACCGCA TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC
541 AAGCTGGAGT ACAACTACAA CAGCCACAAC GTCTATCTCA CCGCCTGAGC GGCCGCGGGG
601 ATCCAGACAT GATAAGATAC ATTGATGAGT TTGGACAAAC CACAACTAGA ATGCAGTGAA
661 AAAAATGCTT TATTTGTGAA ATTTGTGATG CTATTGCTTT ATTTGTAACC ATTATAAGCT
721 GCAATAAACA AGTTAACAAC AACAATTGCA TTCATTTTAT GTTTCAGGTT CAGGGGGAGG
781 TGTGGGAGGT TTTTTCGGAT CCTCTAGAGT CGATCTGCAG GCATGCTAGC TTGGCGTAAT
841 CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC
901 GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA
961 TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT
1021 GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC
1081 TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG
1141 CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG
1201 GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC
1261 GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG
1321 GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA
1381 CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC
1441 ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG
1501 TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT
1561 CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA
1621 GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA
1681 CTAGAAGGAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG
1741 TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA
1801 AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG
1861 GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA
1921 AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA
1981 TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG
2041 CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA
2101 TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC
2161 CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC
2221 CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA
2281 GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC
2341 GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT
2401 GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA
2461 GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG
2521 TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG
2581 AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC
2641 CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT
2701 CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT
2761 CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG
2821 CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC
2881 AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA
2941 TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG
3001 TCTAAGAAAC CATTATTATC ATGACATTAA CCTATAAAAA TAGGCGTATC ACGAGGCCCT
3061 TTCGTCTCGC GCGTTTCGGT GATGACGGTG AAAACCTCTG ACACATGCAG CTCCCGGAGA
3121 CGGTCACAGC TTGTCTGTAA GCGGATGCCG GGAGCAGACA AGCCCGTCAG GGCGCGTCAG
3181 CGGGTGTTGG CGGGTGTCGG GGCTGGCTTA ACTATGCGGC ATCAGAGCAG ATTGTACTGA
3241 GAGTGCACCA TATGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA
3301 GGCGCCATTC GCCATTCAGG CTGCGCAACT GTTGGGAAGG GCGATCGGTG CGGGCCTCTT
3361 CGCTATTACG CCAGCTGGCG AAAGGGGGAT GTGCTGCAAG GCGATTAAGT TGGGTAACGC
3421 CAGGGTTTTC CCAGTCACGA CGTTGTAAAA CGACGGCCAG TGAGTTCGAG CTTGCATGCC
3481 TGCAGGTCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC
3541 GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT
3601 GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC
3661 ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG
3721 CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG
3781 CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT
3841 CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA
3901 ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA
3961 GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTCGTTT AGTGAACCGT CAGATCGCCT
4021 GGAGACGCCA TCCACGCTGT TTTGACCTCC ATAGAAGACA CCGGGACCGA TCCAGCCTCC
4081 GGACTCTAGA GGATCCGGTA CTAGAGGAAC TGAAAAACCA GAAAGTTAAC TGGTAAGTTT
4141 AGTCTTTTTG TCTTTTATTT CAGGTCCCGG ATCCGGTGGT GGTGCAAATC AAAGAACTGC
4201 TCCTCAGTGG ATGTTGCCTT TACTTCTAGG CCTGTACGGA AGTGTTACTT CTGCTCTAAA
4261 AGCTGCGGAA TTGTACCCGC GGGCCCACC
//
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