质粒名称: | pBiFC-CC155 |
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出品公司: | 爱迪基因 |
目录编号: | 22015 |
存储实验室: | Chang-Deng Hu |
载体骨架基本信息 |
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载体名称: | pCMV-HA |
载体抗性: | 氨苄霉素 |
空载体大小: | 3833 bp |
载体类型: | 哺乳细胞表达 |
高拷贝/低拷贝: | 高拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | CC155 |
物种来源: | 维多利亚水母 |
插入大小: | 255 bp |
基因突变: | N/A |
标签/融合蛋白: | HA(N端) |
克隆方法: | 限制性内切酶 |
5' 克隆位点: | KpnI(未破坏) |
3' 克隆位点: | NotI (未破坏) |
5' 测序引物 | CMV-F |
3' 测序引物 | EBV-Rev |
文献引用方法 |
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材料和方法部分: | pBiFC-CC155 was a gift from Chang-Deng Hu (Addgene plasmid # 22015) |
参考文献部分: | Identification of new fluorescent protein fragments for bimolecular fluorescence complementation analysis under physiological conditions. Shyu YJ, Liu H, Deng X, Hu CD. Biotechniques. 2006 Jan . 40(1):61-6. 10.2144/000112036 PubMed 16454041 |
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载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pBiFC-CC155 4088 bp DNA SYN DEFINITION pBiFC-CC155 ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..4088 /organism="pBiFC-CC155" /mol_type="other DNA" misc_feature 31..318 /label="CAG_enhancer" promoter 36..528 /label="CMV_immearly_promoter" misc_feature 485..505 /label="CMV_fwd_primer" promoter 486..555 /label="CMV_promoter" promoter 498..617 /label="CMV2_promoter" misc_feature 529..548 /label="pCEP_fwd_primer" misc_feature 531..555 /label="LNCX_primer" misc_feature 631..657 /label="pCMV_primer" misc_feature 672..768 /label="modSV40_late_16s_int" misc_feature 672..702 /label="SV40_late_19s_int" CDS 750..1286 /label="ORF frame 3" gene 967..1212 /label="ECFP (468 - 713)" /gene="ECFP (468 - 713)" misc_feature 1149..1170 /label="EGFP_C_primer" terminator complement(1237..1356) /label="SV40_PA_terminator" misc_feature complement(1251..1270) /label="EBV_rev_primer" promoter complement(1468..1486) /label="M13_reverse_primer" misc_feature complement(1485..1507) /label="M13_pUC_rev_primer" promoter complement(1521..1550) /label="lac_promoter" gene complement(2633..3493) /label="Ampicillin" /gene="Ampicillin" CDS complement(2633..3493) /label="ORF frame 2" promoter complement(3535..3563) /label="AmpR_promoter" misc_feature complement(3722..3744) /label="pGEX_3_primer" misc_feature complement(3943..4086) /label="lacZ_a" misc_feature 4057..4079 /label="M13_pUC_fwd_primer" promoter 4072..4088 /label="M13_forward20_primer" ORIGIN 1 GAGTTCGAGC TTGCATGCCT GCAGGTCGTT ACATAACTTA CGGTAAATGG CCCGCCTGGC 61 TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAATGA CGTATGTTCC CATAGTAACG 121 CCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT TACGGTAAAC TGCCCACTTG 181 GCAGTACATC AAGTGTATCA TATGCCAAGT ACGCCCCCTA TTGACGTCAA TGACGGTAAA 241 TGGCCCGCCT GGCATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC 301 ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG 361 CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG 421 AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAACAA CTCCGCCCCA 481 TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT ATATAAGCAG AGCTCGTTTA 541 GTGAACCGTC AGATCGCCTG GAGACGCCAT CCACGCTGTT TTGACCTCCA TAGAAGACAC 601 CGGGACCGAT CCAGCCTCCG GACTCTAGAG GATCCGGTAC TAGAGGAACT GAAAAACCAG 661 AAAGTTAACT GGTAAGTTTA GTCTTTTTGT CTTTTATTTC AGGTCCCGGA TCCGGTGGTG 721 GTGCAAATCA AAGAACTGCT CCTCAGTGGA TGTTGCCTTT ACTTCTAGGC CTGTACGGAA 781 GTGTTACTTC TGCTCTAAAA GCTGCGGAAT TGTACCCGCG GGCCCACCAT GTACCCATAC 841 GATGTTCCAG ATTACGCTCT TATGGCCATG GAGGCCCGAA TTCGGTCGAC CGAGATCTCT 901 CGAGGTACCC GTCCGGCGTG CAAAATCCCG AACGACCTGA AACAGAAAGT CATGAACCAC 961 GACAAGCAGA AGAACGGCAT CAAGGCCAAC TTCAAGATCC GCCACAACAT CGAGGACGGC 1021 AGCGTGCAGC TCGCCGACCA CTACCAGCAG AACACCCCCA TCGGCGACGG CCCCGTGCTG 1081 CTGCCCGACA ACCACTACCT GAGCACCCAG TCCGCCCTGA GCAAAGACCC CAACGAGAAG 1141 CGCGATCACA TGGTCCTGCT GGAGTTCGTG ACCGCCGCCG GGATCACTCT CGGCATGGAC 1201 GAGCTGTACA AGTAAGCGGC CGCGGGGATC CAGACATGAT AAGATACATT GATGAGTTTG 1261 GACAAACCAC AACTAGAATG CAGTGAAAAA AATGCTTTAT TTGTGAAATT TGTGATGCTA 1321 TTGCTTTATT TGTAACCATT ATAAGCTGCA ATAAACAAGT TAACAACAAC AATTGCATTC 1381 ATTTTATGTT TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT TTCGGATCCT CTAGAGTCGA 1441 TCTGCAGGCA TGCTAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT 1501 ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG 1561 CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG 1621 GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC 1681 GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC 1741 GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA 1801 ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG 1861 CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT 1921 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA 1981 GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC 2041 TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT 2101 AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG 2161 CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG 2221 CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT 2281 TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC 2341 TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG 2401 CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC 2461 AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT 2521 AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA 2581 AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT 2641 GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT 2701 GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG 2761 CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG 2821 CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA 2881 ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG 2941 CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG 3001 GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT 3061 CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA 3121 TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG 3181 GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC 3241 CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG 3301 GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA 3361 TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG 3421 GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT 3481 GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC 3541 TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA 3601 CATTTCCCCG AAAAGTGCCA CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT 3661 ATAAAAATAG GCGTATCACG AGGCCCTTTC GTCTCGCGCG TTTCGGTGAT GACGGTGAAA 3721 ACCTCTGACA CATGCAGCTC CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA 3781 GCAGACAAGC CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC TGGCTTAACT 3841 ATGCGGCATC AGAGCAGATT GTACTGAGAG TGCACCATAT GCGGTGTGAA ATACCGCACA 3901 GATGCGTAAG GAGAAAATAC CGCATCAGGC GCCATTCGCC ATTCAGGCTG CGCAACTGTT 3961 GGGAAGGGCG ATCGGTGCGG GCCTCTTCGC TATTACGCCA GCTGGCGAAA GGGGGATGTG 4021 CTGCAAGGCG ATTAAGTTGG GTAACGCCAG GGTTTTCCCA GTCACGACGT TGTAAAACGA 4081 CGGCCAGT //
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