出品公司: | Invitrogen |
---|---|
载体名称: | pHOOK1 |
质粒类型: | 哺乳动物表达载体;Capture-Tec系统;免疫磁珠 |
高拷贝/低拷贝: | 高拷贝 |
克隆方法: | 限制性内切酶,多克隆位点 |
启动子: | CMV |
载体大小: | 6061 bp |
5' 测序引物及序列: | CMV fwd 5’ CGCAAATGGGCGGTAGGCGTG3’ |
3' 测序引物及序列: | SV40pA rev 5'GAAATTTGTGATGCTATTGC' |
载体标签: | IgK、HA tag (N-term);2XMyc tag (N-term) |
载体抗性: | 氨苄青霉素 |
筛选标记: | 博来霉素 |
克隆菌株: | DH5α 等 |
宿主细胞(系): | 哺乳动物细胞 |
备注: |
pHOOK1载体可以用于: 1. The effect of dominant, negative mutations of growth regulating proteins or toxic gene products 2. The acute effects of constitutive mutations 3. The effect of anti-sense RNA or ribozymes 4. Temporal events along a developmental or signaling pathway 5. Activation or inactivation of downstream genes using differential display 6. You may also use the pHook-3 System to isolate a homogeneous population of transfected cells for: a) Immunoblot analysis of proteins b) Isolation of DNA or RNA c) Flow Cytometry See pHOOK-3 |
产品目录号: | V690-20 |
稳定性: | 瞬表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 非病毒 |
买家导航
pHOOK1载体质粒基本信息
pHOOK1质粒图谱载体图谱和pHOOK1载体序列质粒序列多克隆位点信息
pHOOK1质粒载体简介
pHOOK1质粒序列载体序列
LOCUS Exported 6061 bp ds-DNA circular SYN 12-AUG-2016 KEYWORDS pHOOK1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6061) AUTHORS Biofeng Lab. FEATURES Location/Qualifiers source 1..6061 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 10..389 /note="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" promoter 390..593 /note="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" promoter 638..656 /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" misc_feature 664..732 /note="MCS" CDS 737..799 /codon_start=1 /product="leader sequence from mouse immunoglobulin kappa light chain" /note="Ig-kappa leader" /translation="METDTLLLWVLLLWVPGSTGD" CDS 800..826 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /note="HA" /translation="YPYDVPDYA" misc_feature 827..1564 /note="phOx sFv" CDS 1565..1594 /codon_start=1 /product="Myc (human c-Myc oncogene) epitope tag" /note="Myc" /translation="EQKLISEEDL" CDS 1610..1639 /codon_start=1 /product="Myc (human c-Myc oncogene) epitope tag" /note="Myc" /translation="EQKLISEEDL" polyA_signal 1817..2041 /note="bGH poly(A)" /note="bovine growth hormone polyadenylation signal" rep_origin complement(2173..2761) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" polyA_signal 3090..3137 /note="HSV TK poly(A)" /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" promoter complement(4192..4552) /note="SV40 promoter" /note="SV40 enhancer and early promoter" rep_origin 4206..4344 /note="SV40 ori" /note="SV40 origin of replication" CDS complement(4612..5472) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(5473..5577) /gene="bla" /note="AmpR promoter" rep_origin 5604..6059 /direction=RIGHT /note="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" ORIGIN 1 gcgcgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag 61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct 121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc 181 caatagggac tttccattga cgtcaatggg tggactattt acggtaaact gcccacttgg 241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat 301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc 601 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga 661 cccaagcttg gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgg 721 cttggggata tccaccatgg agacagacac actcctgcta tgggtactgc tgctctgggt 781 tccaggttcc actggtgact atccatatga tgttccagat tatgctgggg cccagccggc 841 catggccgag gtcaagctgc aggagtcagg gggaggctta gtgcagcctg gagggtcccg 901 gaaactctcc tgtgcagcct ctggattcac tttcagtagc tttggaatgc actgggttcg 961 tcaggctcca gagaaggggc tggagtgggt cgcatatatt agtagtggca gtagtaccat 1021 ctactatgca gacacagtga agggacgatt caccatctcc agagacaatc ccaagaacac 1081 cctgttcctg caaatgacca gtctaaggtc tgaggacacg gncatgtatt actgtgcaag 1141 agattacggg gcttattggg gccaagggac cacggncacc gtctcctcag gtggaggcgg 1201 ctcaggcgga ggtggctctg gcggtggcgg atcggacatt gagctcaccc agtctccagc 1261 aatcatgtct gcatctccag gggagagggt caccatgacc tgcagtgcca gttcaagtgt 1321 aaggtacatg aactggttcc aacagaagtc aggcacctcc cccaaaagat ggatttatga 1381 cacatccaaa ctgtcttctg gagtccctgc tcgcttcagt ggcagtgggt ctgggacctc 1441 ttactctctc acaatcagca gcatggaggc tgaagatgct gccacttact actgccagca 1501 gtggagtagt aacccactca cgttcggtgc tgggaccaag ctggagctga aacgggcggc 1561 cgcagaacaa aaactcatct cagaagagga tctgaatggg gccgtcgacg aacaaaaact 1621 catctcagaa gaggatctga atgctgtggg ccaggacacg caggaggtca tcgtggtgcc 1681 acactccttg ccctttaagg tggtggtgat ctcagccatc ctggccctgg tggtgctcac 1741 catcatctcc cttatcatcc tcatcatgct ttggcagaag aagccacgtt aggcggccgc 1801 tcgagatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc 1861 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa 1921 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac 1981 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg 2041 gcttctgagg cggaaagaac cagtggcggt aatacggtta tccacagaat caggggataa 2101 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 2161 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 2221 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 2281 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 2341 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 2401 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 2461 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 2521 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 2581 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct 2641 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 2701 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 2761 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 2821 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 2881 atgaagtttt aaatcaatct aaagtatata tgagtaacct gaggctatgg cagggcctgc 2941 cgccccgacg ttggctgcga gccctgggcc ttcacccgaa cttggggggt ggggtgggga 3001 aaaggaagaa acgcgggcgt attggcccca atggggtctc ggtggggtat cgacagagtg 3061 ccagccctgg gaccgaaccc cgcgtttatg aacaaacgac ccaacaccgt gcgttttatt 3121 ctgtcttttt attgccgtca tagcgcgggt tccttccggt attgtctcct tccgtgtttc 3181 agttagcctc cccctagggt gggcgaagaa ctccagcatg agatccccgc gctggaggat 3241 catccagccg gcgtcccgga aaacgattcc gaagcccaac ctttcataga aggcggcggt 3301 ggaatcgaaa tctcgtgatg gcaggttggg cgtcgcttgg tcggtcattt cgaaccccag 3361 agtcccgctc agaagaactc gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga 3421 gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ctcttcagca 3481 atatcacggg tagccaacgc tatgtcctga tagcggtccg ccacacccag ccggccacag 3541 tcgatgaatc cagaaaagcg gccattttcc accatgatat tcggcaagca ggcatcgcca 3601 tgggtcacga cgagatcctc gccgtcgggc atgctcgcct tgagcctggc gaacagttcg 3661 gctggcgcga gcccctgatg ctcttgatca tcctgatcga caagaccggc ttccatccga 3721 gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga atgggcaggt agccggatca 3781 agcgtatgca gccgccgcat tgcatcagcc atgatggata ctttctcggc aggagcaagg 3841 tgagatgaca ggagatcctg ccccggcact tcgcccaata gcagccagtc ccttcccgct 3901 tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc 3961 cgcgctgcct cgtcttgcag ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga 4021 accgggcgcc cctgcgctga cagccggaac acggcggcat cagagcagcc gattgtctgt 4081 tgtgcccagt catagccgaa tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat 4141 ccatcttgtt caatcatgcg aaacgatcct catcctgtct cttgatcgat ctttgcaaaa 4201 gcctaggcct ccaaaaaagc ctcctcacta cttctggaat agctcagagg ccgaggaggc 4261 ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca tggggcggag aatgggcgga 4321 actgggcgga gttaggggcg ggatgggcgg agttaggggc gggactatgg ttgctgacta 4381 attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact ttccacacct 4441 ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga 4501 ctttccacac cctaactgac acacattcca cagctggttc tttccgcctc aggactcttc 4561 ctttttcaat aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg 4621 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg 4681 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc 4741 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc 4801 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa 4861 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc 4921 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg 4981 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 5041 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat 5101 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg 5161 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc 5221 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 5281 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 5341 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 5401 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 5461 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 5521 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 5581 atttccccga aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt 5641 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc 5701 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg 5761 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta 5821 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt 5881 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat 5941 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa 6001 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta 6061 c //
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pHOOK1质粒载体应用举例
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