| 出品公司: | Invitrogen |
|---|---|
| 载体名称: | pHOOK1 |
| 质粒类型: | 哺乳动物表达载体；Capture-Tec系统；免疫磁珠 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | CMV |
| 载体大小: | 6061 bp |
| 5' 测序引物及序列: | CMV fwd 5’ CGCAAATGGGCGGTAGGCGTG3’ |
| 3' 测序引物及序列: | SV40pA rev 5'GAAATTTGTGATGCTATTGC' |
| 载体标签: | IgK、HA tag (N-term)；2XMyc tag (N-term) |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 博来霉素 |
| 克隆菌株: | DH5α 等 |
| 宿主细胞（系）: | 哺乳动物细胞 |
| 备注: |
pHOOK1载体可以用于： 1. The effect of dominant, negative mutations of growth regulating proteins or toxic gene products 2. The acute effects of constitutive mutations 3. The effect of anti-sense RNA or ribozymes 4. Temporal events along a developmental or signaling pathway 5. Activation or inactivation of downstream genes using differential display 6. You may also use the pHook-3 System to isolate a homogeneous population of transfected cells for: a) Immunoblot analysis of proteins b) Isolation of DNA or RNA c) Flow Cytometry See pHOOK-3 |
| 产品目录号: | V690-20 |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS Exported 6061 bp ds-DNA circular SYN 12-AUG-2016
KEYWORDS pHOOK1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6061)
AUTHORS Biofeng Lab.
FEATURES Location/Qualifiers
source 1..6061
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 10..389
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 390..593
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 638..656
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature 664..732
/note="MCS"
CDS 737..799
/codon_start=1
/product="leader sequence from mouse immunoglobulin kappa
light chain"
/note="Ig-kappa leader"
/translation="METDTLLLWVLLLWVPGSTGD"
CDS 800..826
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/note="HA"
/translation="YPYDVPDYA"
misc_feature 827..1564
/note="phOx sFv"
CDS 1565..1594
/codon_start=1
/product="Myc (human c-Myc oncogene) epitope tag"
/note="Myc"
/translation="EQKLISEEDL"
CDS 1610..1639
/codon_start=1
/product="Myc (human c-Myc oncogene) epitope tag"
/note="Myc"
/translation="EQKLISEEDL"
polyA_signal 1817..2041
/note="bGH poly(A)"
/note="bovine growth hormone polyadenylation signal"
rep_origin complement(2173..2761)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
polyA_signal 3090..3137
/note="HSV TK poly(A)"
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
promoter complement(4192..4552)
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 4206..4344
/note="SV40 ori"
/note="SV40 origin of replication"
CDS complement(4612..5472)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5473..5577)
/gene="bla"
/note="AmpR promoter"
rep_origin 5604..6059
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
ORIGIN
1 gcgcgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
181 caatagggac tttccattga cgtcaatggg tggactattt acggtaaact gcccacttgg
241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc
601 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga
661 cccaagcttg gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgg
721 cttggggata tccaccatgg agacagacac actcctgcta tgggtactgc tgctctgggt
781 tccaggttcc actggtgact atccatatga tgttccagat tatgctgggg cccagccggc
841 catggccgag gtcaagctgc aggagtcagg gggaggctta gtgcagcctg gagggtcccg
901 gaaactctcc tgtgcagcct ctggattcac tttcagtagc tttggaatgc actgggttcg
961 tcaggctcca gagaaggggc tggagtgggt cgcatatatt agtagtggca gtagtaccat
1021 ctactatgca gacacagtga agggacgatt caccatctcc agagacaatc ccaagaacac
1081 cctgttcctg caaatgacca gtctaaggtc tgaggacacg gncatgtatt actgtgcaag
1141 agattacggg gcttattggg gccaagggac cacggncacc gtctcctcag gtggaggcgg
1201 ctcaggcgga ggtggctctg gcggtggcgg atcggacatt gagctcaccc agtctccagc
1261 aatcatgtct gcatctccag gggagagggt caccatgacc tgcagtgcca gttcaagtgt
1321 aaggtacatg aactggttcc aacagaagtc aggcacctcc cccaaaagat ggatttatga
1381 cacatccaaa ctgtcttctg gagtccctgc tcgcttcagt ggcagtgggt ctgggacctc
1441 ttactctctc acaatcagca gcatggaggc tgaagatgct gccacttact actgccagca
1501 gtggagtagt aacccactca cgttcggtgc tgggaccaag ctggagctga aacgggcggc
1561 cgcagaacaa aaactcatct cagaagagga tctgaatggg gccgtcgacg aacaaaaact
1621 catctcagaa gaggatctga atgctgtggg ccaggacacg caggaggtca tcgtggtgcc
1681 acactccttg ccctttaagg tggtggtgat ctcagccatc ctggccctgg tggtgctcac
1741 catcatctcc cttatcatcc tcatcatgct ttggcagaag aagccacgtt aggcggccgc
1801 tcgagatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
1861 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
1921 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
1981 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
2041 gcttctgagg cggaaagaac cagtggcggt aatacggtta tccacagaat caggggataa
2101 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
2161 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
2221 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
2281 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
2341 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
2401 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
2461 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
2521 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
2581 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
2641 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
2701 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
2761 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
2821 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
2881 atgaagtttt aaatcaatct aaagtatata tgagtaacct gaggctatgg cagggcctgc
2941 cgccccgacg ttggctgcga gccctgggcc ttcacccgaa cttggggggt ggggtgggga
3001 aaaggaagaa acgcgggcgt attggcccca atggggtctc ggtggggtat cgacagagtg
3061 ccagccctgg gaccgaaccc cgcgtttatg aacaaacgac ccaacaccgt gcgttttatt
3121 ctgtcttttt attgccgtca tagcgcgggt tccttccggt attgtctcct tccgtgtttc
3181 agttagcctc cccctagggt gggcgaagaa ctccagcatg agatccccgc gctggaggat
3241 catccagccg gcgtcccgga aaacgattcc gaagcccaac ctttcataga aggcggcggt
3301 ggaatcgaaa tctcgtgatg gcaggttggg cgtcgcttgg tcggtcattt cgaaccccag
3361 agtcccgctc agaagaactc gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga
3421 gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ctcttcagca
3481 atatcacggg tagccaacgc tatgtcctga tagcggtccg ccacacccag ccggccacag
3541 tcgatgaatc cagaaaagcg gccattttcc accatgatat tcggcaagca ggcatcgcca
3601 tgggtcacga cgagatcctc gccgtcgggc atgctcgcct tgagcctggc gaacagttcg
3661 gctggcgcga gcccctgatg ctcttgatca tcctgatcga caagaccggc ttccatccga
3721 gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga atgggcaggt agccggatca
3781 agcgtatgca gccgccgcat tgcatcagcc atgatggata ctttctcggc aggagcaagg
3841 tgagatgaca ggagatcctg ccccggcact tcgcccaata gcagccagtc ccttcccgct
3901 tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc
3961 cgcgctgcct cgtcttgcag ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga
4021 accgggcgcc cctgcgctga cagccggaac acggcggcat cagagcagcc gattgtctgt
4081 tgtgcccagt catagccgaa tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat
4141 ccatcttgtt caatcatgcg aaacgatcct catcctgtct cttgatcgat ctttgcaaaa
4201 gcctaggcct ccaaaaaagc ctcctcacta cttctggaat agctcagagg ccgaggaggc
4261 ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca tggggcggag aatgggcgga
4321 actgggcgga gttaggggcg ggatgggcgg agttaggggc gggactatgg ttgctgacta
4381 attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact ttccacacct
4441 ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga
4501 ctttccacac cctaactgac acacattcca cagctggttc tttccgcctc aggactcttc
4561 ctttttcaat aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
4621 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
4681 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
4741 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
4801 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
4861 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
4921 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
4981 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
5041 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
5101 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
5161 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
5221 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
5281 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
5341 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
5401 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
5461 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
5521 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
5581 atttccccga aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
5641 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
5701 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
5761 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
5821 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
5881 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
5941 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
6001 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta
6061 c
//
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