出品公司: | Invitrogen |
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载体名称: | pEBVHis-lacZ |
质粒类型: | 哺乳动物表达载体;附加体载体 |
高拷贝/低拷贝: | 高拷贝 |
克隆方法: | 限制性内切酶,多克隆位点 |
启动子: | RSV |
载体大小: | 13328 bp |
5' 测序引物及序列: | pREP Forward 5’ GCTCGATACAATAAACGCC 3’ |
3' 测序引物及序列: | SV40pA rev 5'GAAATTTGTGATGCTATTGC' |
载体标签: | His tag (N-term) |
载体抗性: | 氨苄青霉素 |
筛选标记: | Hygromycin |
克隆菌株: | DH5α 等 |
宿主细胞(系): | 人类细胞;灵长类细胞;犬类细胞 |
备注: |
pEBVHis-lacZ载体是附加体型载体,可以在基因组外自主复制,不需要整合和转座到基因组上,无需包装成病毒,即可实现稳定表达。其它附加体载体有: pREP4 PCEP4 pREP7 pREP10 pEBNA-DEST pEBVHisA pEBVHisB pEBVHisC pREP4/CAT pEBVHis-lacZ |
产品目录号: | -- |
稳定性: | 稳表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 非病毒 |
买家导航
pEBVHis-lacZ载体质粒基本信息
pEBVHis-lacZ质粒图谱载体图谱和pEBVHis-lacZ载体序列质粒序列多克隆位点信息
pEBVHis-lacZ质粒载体简介
pEBVHis-lacZ质粒序列载体序列
LOCUS Exported 13328 bp ds-DNA circular SYN 13-AUG-2016 DEFINITION synthetic circular DNA KEYWORDS pEBVHis-lacz SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 13328) AUTHORS Biofeng Lab. FEATURES Location/Qualifiers source 1..13328 /organism="synthetic DNA construct" /mol_type="other DNA" polyA_signal 276..410 /note="SV40 poly(A)" /note="SV40 polyadenylation signal" CDS complement(453..3500) /codon_start=1 /gene="lacZ" /product="beta-galactosidase" /note="lacZ" /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS LNGEWRFAWFPVPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD SWSPSVSAEFQLSAGRYHYQLVWCQK" CDS complement(3519..3542) /codon_start=1 /product="Xpress(TM) epitope tag, including an enterokinase recognition and cleavage site" /note="Xpress(TM) tag" /translation="DLYDDDDK" CDS complement(3546..3578) /codon_start=1 /product="leader peptide from bacteriophage T7 gene 10" /note="T7 tag (gene 10 leader)" /note="promotes efficient translation in E. coli" /translation="MASMTGGQQMG" CDS complement(3582..3599) /codon_start=1 /product="6xHis affinity tag" /note="6xHis" /translation="HHHHHH" promoter complement(3615..3877) /note="RSV promoter" /note="Rous sarcoma virus enhancer/promoter" polyA_signal 4647..4695 /note="HSV TK poly(A)" /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" CDS complement(4760..5785) /codon_start=1 /gene="aph(4)-Ia" /product="aminoglycoside phosphotransferase from E. coli" /note="HygR" /note="confers resistance to hygromycin" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK E" promoter complement(5825..5970) /note="HSV TK promoter" /note="herpes simplex virus thymidine kinase promoter" rep_origin complement(6587..7175) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(7346..8206) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(8207..8311) /gene="bla" /note="AmpR promoter" rep_origin 11065..12854 /note="oriP" /note="Epstein-Barr virus oriP replication origin (Yates et al., 2000)" ORIGIN 1 tctagagtcg accggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct 61 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct 121 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagccg gatcataatc 181 agccatacca catttgtaga ggttttactt gctttaaaaa acctccccac ctccccctga 241 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg 301 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt 361 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc gatccggcct 421 tgccggcctc gatcgagcgg ccgctagcaa gcttattttt gacaccagac caactggtaa 481 tggtagcgac cggcgctcag ctggaattcc gccgatactg acgggctcca ggagtcgtcg 541 ccaccaatcc ccatatggaa accgtcgata ttcagccatg tgccttcttc cgcgtgcagc 601 agatggcgat ggctggtttc catcagttgc tgttgactgt agcggctgat gttgaactgg 661 aagtcgccgc gccactggtg tgggccataa ttcaattcgc gcgtcccgca gcgcagaccg 721 ttttcgctcg ggaagacgta cggggtatac atgtctgaca atggcagatc ccagcggtca 781 aaacaggcgg cagtaaggcg gtcgggatag ttttcttgcg gccctaatcc gagccagttt 841 acccgctctg ctacctgcgc cagctggcag ttcaggccaa tccgcgccgg atgcggtgta 901 tcgctcgcca cttcaacatc aacggtaatc gccatttgac cactaccatc aatccggtag 961 gttttccggc tgataaataa ggttttcccc tgatgctgcc acgcgtgagc ggtcgtaatc 1021 agcaccgcat cagcaagtgt atctgccgtg cactgcaaca acgctgcttc ggcctggtaa 1081 tggcccgccg ccttccagcg ttcgacccag gcgttagggt caatgcgggt cgcttcactt 1141 acgccaatgt cgttatccag cggtgcacgg gtgaactgat cgcgcagcgg cgtcagcagt 1201 tgttttttat cgccaatcca catctgtgaa agaaagcctg actggcggtt aaattgccaa 1261 cgcttattac ccagctcgat gcaaaaatcc atttcgctgg tggtcagatg cgggatggcg 1321 tgggacgcgg cggggagcgt cacactgagg ttttccgcca gacgccactg ctgccaggcg 1381 ctgatgtgcc cggcttctga ccatgcggtc gcgttcggtt gcactacgcg tactgtgagc 1441 cagagttgcc cggcgctctc cggctgcggt agttcaggca gttcaatcaa ctgtttacct 1501 tgtggagcga catccagagg cacttcaccg cttgccagcg gcttaccatc cagcgccacc 1561 atccagtgca ggagctcgtt atcgctatga cggaacaggt attcgctggt cacttcgatg 1621 gtttgcccgg ataaacggaa ctggaaaaac tgctgctggt gttttgcttc cgtcagcgct 1681 ggatgcggcg tgcggtcggc aaagaccaga ccgttcatac agaactggcg atcgttcggc 1741 gtatcgccaa aatcaccgcc gtaagccgac cacgggttgc cgttttcatc atatttaatc 1801 agcgactgat ccacccagtc ccagacgaag ccgccctgta aacggggata ctgacgaaac 1861 gcctgccagt atttagcgaa accgccaaga ctgttaccca tcgcgtgggc gtattcgcaa 1921 aggatcagcg ggcgcgtctc tccaggtagc gaaagccatt ttttgatgga ccatttcggc 1981 acagccggga agggctggtc ttcatccacg cgcgcgtaca tcgggcaaat aatatcggtg 2041 gccgtggtgt cggctccgcc gccttcatac tgcaccgggc gggaaggatc gacagatttg 2101 atccagcgat acagcgcgtc gtgattagcg ccgtggcctg attcattccc cagcgaccag 2161 atgatcacac tcgggtgatt acgatcgcgc tgcaccattc gcgttacgcg ttcgctcatc 2221 gccggtagcc agcgcggatc atcggtcaga cgattcattg gcaccatgcc gtgggtttca 2281 atattggctt catccaccac atacaggccg tagcggtcgc acagcgtgta ccacagcgga 2341 tggttcggat aatgcgaaca gcgcacggcg ttaaagttgt tctgcttcat cagcaggata 2401 tcctgcacca tcgtctgctc atccatgacc tgaccatgca gaggatgatg ctcgtgacgg 2461 ttaacgcctc gaatcagcaa cggcttgccg ttcagcagca gcagaccatt ttcaatccgc 2521 acctcgcgga aaccgacatc gcaggcttct gcttcaatca gcgtgccgtc ggcggtgtgc 2581 agttcaacca ccgcacgata gagattcggg atttcggcgc tccacagttt cgggttttcg 2641 acgttcagac gtagtgtgac gcgatcggca taaccaccac gctcatcgat aatttcaccg 2701 ccgaaaggcg cggtgccgct ggcgacctgc gtttcaccct gccataaaga aactgttacc 2761 cgtaggtagt cacgcaactc gccgcacatc tgaacttcag cctccagtac agcgcggctg 2821 aaatcatcat taaagcgagt ggcaacatgg aaatcgctga tttgtgtagt cggtttatgc 2881 agcaacgaga cgtcacggaa aatgccgctc atccgccaca tatcctgatc ttccagataa 2941 ctgccgtcac tccaacgcag caccatcacc gcgaggcggt tttctccggc gcgtaaaaat 3001 gcgctcaggt caaattcaga cggcaaacga ctgtcctggc cgtaaccgac ccagcgcccg 3061 ttgcaccaca gatgaaacgc cgagttaacg ccatcaaaaa taattcgcgt ctggccttcc 3121 tgtagccagc tttcatcaac attaaatgtg agcgagtaac aacccgtcgg attctccgtg 3181 ggaacaaacg gcggattgac cgtaatggga taggttacgt tggtgtagat gggcgcatcg 3241 taaccgtgca tctgccagtt tgaggggacg acgacagtat cggcctcagg aagatcgcac 3301 tccagccagc tttccggcac cgcttctggt accggaaacc aggcaaagcg ccattcgcca 3361 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag 3421 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag 3481 tcacgacgtt gtaaaacgac gggatctatc attggatcct tatcgtcatc gtcgtacaga 3541 tcccgaccca tttgctgtcc accagtcatg ctagccatac catgatgatg atgatgatga 3601 gaacccccca tggaggtgca caccaatgtg gtgaatggtc aaatggcgtt tattgtatcg 3661 agctaggcac ttaaatacaa ttatctctgc aatgcggaat tcagtggttc gtccaatcca 3721 tgtcagacct gtctgttgcc ttcctaataa ggcacgatcg taccacctta cttccaccaa 3781 tcggcatgca cggtgctttt tctctccttg taaggcatgt tgctaactca tcgttaccat 3841 gttgcaagac tacaagtgta ttgcataaga ctacatttcc ccctccctat gcaaaagcga 3901 aactactata tcctgagggg actcctaacc gcgtacaacc gaagccccgc tgggcgccta 3961 aacacaccct agtcccctca gatacgcgta tatctggccc gtacatcgcg aagcagcgca 4021 aaacgcctaa ccctaagcag attcttcatg caattgtcgg tcaagccttg ccttgttgta 4081 gcttaaattt tgctcgcgca ctactcagcg acctccaaca cacaagcagg gagcagatac 4141 tggcttaact atgcggcagc agagcagatt gtactgagag tgcaccatac aagctcagga 4201 tctgcgatga taagctgtca aacatgagaa ttggtcgacc actgggcgcc agaaatccgc 4261 gcggtggttt ttgggggtcg ggggtgtttg gcagccacag acgcccggtg ttcgtgtcgc 4321 gccagtacat gcggtccatg cccaggccat ccaaaaacca tgggtctgtc tgctcagtcc 4381 agtcgtggac cagaccccac gcaacgccca aaataataac ccccacgaac cataaaccat 4441 tccccatggg ggaccccgtc cctaacccac ggggccagtg gctatggcag ggcctgccgc 4501 cccgacgttg gctgcgagcc ctgggccttc acccgaactt ggggggtggg gtggggaaaa 4561 ggaagaaacg cgggcgtatt ggccccaatg gggtctcggt ggggtatcga cagagtgcca 4621 gccctgggac cgaaccccgc gtttatgaac aaacgaccca acacccgtgc gttttattct 4681 gtctttttat tgccgtcata gcgcgggttc cttccggtat tgtctccttc cgtgtttcag 4741 ttagcctccc ccatctcccc tattcctttg ccctcggacg agtgctgggg cgtcggtttc 4801 cactatcggc gagtacttct acacagccat cggtccagac ggccgcgctt ctgcgggcga 4861 tttgtgtacg cccgacagtc ccggctccgg atcggacgat tgcgtcgcat cgaccctgcg 4921 cccaagctgc atcatcgaaa ttgccgtcaa ccaagctctg atagagttgg tcaagaccaa 4981 tgcggagcat atacgcccgg agccgcggcg atcctgcaag ctccggatgc ctccgctcga 5041 agtagcgcgt ctgctgctcc atacaagcca accacggcct ccagaagaag atgttggcga 5101 cctcgtattg ggaatccccg aacatcgcct cgctccagtc aatgaccgct gttatgcggc 5161 cattgtccgt caggacattg ttggagccga aatccgcgtg cacgaggtgc cggacttcgg 5221 ggcagtcctc ggcccaaagc atcagctcat cgagagcctg cgcgacggac gcactgacgg 5281 tgtcgtccat cacagtttgc cagtgataca catggggatc agcaatcgcg catatgaaat 5341 cacgccatgt agtgtattga ccgattcctt gcggtccgaa tgggccgaac ccgctcgtct 5401 ggctaagatc ggccgcagcg atcgcatcca tggcctccgc gaccggctgc agaacagcgg 5461 gcagttcggt ttcaggcagg tcttgcaacg tgacaccctg tgcacggcgg gagatgcaat 5521 aggtcaggct ctcgctgaat tccccaatgt caagcacttc cggaatcggg agcgcggccg 5581 atgcaaagtg ccgataaaca taacgatctt tgtagaaacc atcggcgcag ctatttaccc 5641 gcaggacata tccacgccct cctacatcga agctgaaagc acgagattct tcgccctccg 5701 agagctgcat caggtcggag acgctgtcga acttttcgat cagaaacttc tcgacagacg 5761 tcgcggtgag ttcaggcttt ttcatatctc attgcccggg atctgcggca cgctgttgac 5821 gctgttaagc gggtcgctgc agggtcgctc ggtgttcgag gccacacgcg tcaccttaat 5881 atgcgaagtg gacctgggac cgcgccgccc cgactgcatc tgcgtgttcg aattcgccaa 5941 tgacaagacg ctgggcgggg tttgtgtcat catagaacta aagacatgca aatatatttc 6001 ttccggggac accgccagca aacgcgagca acgggccacg gggatgaagc agggcatggc 6061 ggccgacgcg ctgggctacg tcttgctggc gttcgcgacg cgaggctgga tggccttccc 6121 cattatgatt cttctcgctt ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc 6181 caggcaggta gatgacgacc atcagggaca gcttcaagga tcgctcgcgg ctcttaccag 6241 cctaacttcg atcactggac cgctgatcgt cacggcgatt tatgccgcct cggcgagcac 6301 atggaacggg ttggcatgga ttgtaggcgc cgccctatac cttgtctgcc tccccgcgtt 6361 gcgtcgcggt gcatggagcc gggccacctc gacctgaatg gaagccggcg gcacctcgct 6421 aacggattca ccactccaag aattggagcc aatcaattct tgcggagaac tgtgaatgcg 6481 caaaccaacc cttggcagaa catatccatc gcgtccgcca tctccagcag ccgcacgcgg 6541 cgcagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 6601 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 6661 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 6721 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 6781 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 6841 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 6901 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 6961 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 7021 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 7081 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 7141 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 7201 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 7261 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 7321 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 7381 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 7441 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 7501 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 7561 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 7621 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac 7681 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 7741 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 7801 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 7861 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 7921 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc 7981 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 8041 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 8101 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 8161 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 8221 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 8281 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 8341 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct 8401 ttcgtcttca agaattctca tgtttgacag cttatcatcg cgataagctg atcctcacag 8461 gccgcaccca gcttttcttc cgttgcccca gtagcatctc tgtctggtga ccttgaagag 8521 gaagaggagg ggtcccgaga atccccatcc ctaccgtcca gcaaaaaggg ggacgaggaa 8581 tttgaggcct ggcttgaggc tcaggacgca aatcttgagg atgttcagcg ggagttttcc 8641 gggctgcgag taattggtga tgaggacgag gatggttcgg aggatgggga attttcagac 8701 ctggatctgt ctgacagcga ccatgaaggg gatgagggtg ggggggctgt tggagggggc 8761 aggagtctgc actccctgta ttcactgagc gtcgtctaat aaagatgtct attgatctct 8821 tttagtgtga atcatgtctg acgaggggcc aggtacagga cctggaaatg gcctaggaga 8881 gaagggagac acatctggac cagaaggctc cggcggcagt ggacctcaaa gaagaggggg 8941 tgataaccat ggacgaggac ggggaagagg acgaggacga ggaggcggaa gaccaggagc 9001 cccgggcggc tcaggatcag ggccaagaca tagagatggt gtccggagac cccaaaaacg 9061 tccaagttgc attggctgca aagggaccca cggtggaaca ggagcaggag caggagcggg 9121 aggggcagga gcaggagggg caggagcagg aggaggggca ggagcaggag gaggggcagg 9181 aggggcagga ggggcaggag gggcaggagc aggaggaggg gcaggagcag gaggaggggc 9241 aggaggggca ggaggggcag gagcaggagg aggggcagga gcaggaggag gggcaggagg 9301 ggcaggagca ggaggagggg caggaggggc aggaggggca ggagcaggag gaggggcagg 9361 agcaggagga ggggcaggag gggcaggagc aggaggaggg gcaggagggg caggaggggc 9421 aggagcagga ggaggggcag gagcaggagg ggcaggaggg gcaggagggg caggagcagg 9481 aggggcagga gcaggaggag gggcaggagg ggcaggaggg gcaggagcag gaggggcagg 9541 agcaggaggg gcaggagcag gaggggcagg agcaggaggg gcaggagggg caggagcagg 9601 aggggcagga ggggcaggag caggaggggc aggaggggca ggagcaggag gaggggcagg 9661 aggggcagga gcaggaggag gggcaggagg ggcaggagca ggaggggcag gaggggcagg 9721 agcaggaggg gcaggagggg caggagcagg aggggcagga ggggcaggag caggaggagg 9781 ggcaggagca ggaggggcag gagcaggagg tggaggccgg ggtcgaggag gcagtggagg 9841 ccggggtcga ggaggtagtg gaggccgggg tcgaggaggt agtggaggcc gccggggtag 9901 aggacgtgaa agagccaggg ggggaagtcg tgaaagagcc agggggagag gtcgtggacg 9961 tggagaaaag aggcccagga gtcccagtag tcagtcatca tcatccgggt ctccaccgcg 10021 caggccccct ccaggtagaa ggccattttt ccaccctgta ggggaagccg attattttga 10081 ataccaccaa gaaggtggcc cagatggtga gcctgacgtg cccccgggag cgatagagca 10141 gggccccgca gatgacccag gagaaggccc aagcactgga ccccggggtc agggtgatgg 10201 aggcaggcgc aaaaaaggag ggtggtttgg aaagcatcgt ggtcaaggag gttccaaccc 10261 gaaatttgag aacattgcag aaggtttaag agctctcctg gctaggagtc acgtagaaag 10321 gactaccgac gaaggaactt gggtcgccgg tgtgttcgta tatggaggta gtaagacctc 10381 cctttacaac ctaaggcgag gaactgccct tgctattcca caatgtcgtc ttacaccatt 10441 gagtcgtctc ccctttggaa tggcccctgg acccggccca caacctggcc cgctaaggga 10501 gtccattgtc tgttatttca tggtcttttt acaaactcat atatttgctg aggttttgaa 10561 ggatgcgatt aaggaccttg ttatgacaaa gcccgctcct acctgcaata tcagggtgac 10621 tgtgtgcagc tttgacgatg gagtagattt gcctccctgg tttccaccta tggtggaagg 10681 ggctgccgcg gagggtgatg acggagatga cggagatgaa ggaggtgatg gagatgaggg 10741 tgaggaaggg caggagtgat gtaacttgtt aggagacgcc ctcaatcgta ttaaaagccg 10801 tgtattcccc cgcactaaag aataaatccc cagtagacat catgcgtgct gttggtgtat 10861 ttctggccat ctgtcttgtc accattttcg tcctcccaac atggggcaat tgggcatacc 10921 catgttgtca cgtcactcag ctccgcgctc aacaccttct cgcgttggaa aacattagcg 10981 acatttacct ggtgagcaat cagacatgcg acggctttag cctggcctcc ttaaattcac 11041 ctaagaatgg gagcaaccag catgcaggaa aaggacaagc agcgaaaatt cacgccccct 11101 tgggaggtgg cggcatatgc aaaggatagc actcccactc tactactggg tatcatatgc 11161 tgactgtata tgcatgagga tagcatatgc tacccggata cagattagga tagcatatac 11221 tacccagata tagattagga tagcatatgc tacccagata tagattagga tagcctatgc 11281 tacccagata taaattagga tagcatatac tacccagata tagattagga tagcatatgc 11341 tacccagata tagattagga tagcctatgc tacccagata tagattagga tagcatatgc 11401 tacccagata tagattagga tagcatatgc tatccagata tttgggtagt atatgctacc 11461 cagatataaa ttaggatagc atatactacc ctaatctcta ttaggatagc atatgctacc 11521 cggatacaga ttaggatagc atatactacc cagatataga ttaggatagc atatgctacc 11581 cagatataga ttaggatagc ctatgctacc cagatataaa ttaggatagc atatactacc 11641 cagatataga ttaggatagc atatgctacc cagatataga ttaggatagc ctatgctacc 11701 cagatataga ttaggatagc atatgctatc cagatatttg ggtagtatat gctacccatg 11761 gcaacattag cccaccgtgc tctcagcgac ctcgtgaata tgaggaccaa caaccctgtg 11821 cttggcgctc aggcgcaagt gtgtgtaatt tgtcctccag atcgcagcaa tcgcgcccct 11881 atcttggccc gcccacctac ttatgcaggt attccccggg gtgccattag tggttttgtg 11941 ggcaagtggt ttgaccgcag tggttagcgg ggttacaatc agccaagtta ttacaccctt 12001 attttacagt ccaaaaccgc agggcggcgt gtgggggctg acgcgtgccc ccactccaca 12061 atttcaaaaa aaagagtggc cacttgtctt tgtttatggg ccccattggc gtggagcccc 12121 gtttaatttt cgggggtgtt agagacaacc agtggagtcc gctgctgtcg gcgtccactc 12181 tctttcccct tgttacaaat agagtgtaac aacatggttc acctgtcttg gtccctgcct 12241 gggacacatc ttaataaccc cagtatcata ttgcactagg attatgtgtt gcccatagcc 12301 ataaattcgt gtgagatgga catccagtct ttacggcttg tccccacccc atggatttct 12361 attgttaaag atattcagaa tgtttcattc ctacactagt atttattgcc caaggggttt 12421 gtgagggtta tattggtgtc atagcacaat gccaccactg aaccccccgt ccaaatttta 12481 ttctgggggc gtcacctgaa accttgtttt cgagcacctc acatacacct tactgttcac 12541 aactcagcag ttattctatt agctaaacga aggagaatga agaagcaggc gaagattcag 12601 gagagttcac tgcccgctcc ttgatcttca gccactgccc ttgtgactaa aatggttcac 12661 taccctcgtg gaatcctgac cccatgtaaa taaaaccgtg acagctcatg gggtgggaga 12721 tatcgctgtt ccttaggacc cttttactaa ccctaattcg atagcatatg cttcccgttg 12781 ggtaacatat gctattgaat tagggttagt ctggatagta tatactacta cccgggaagc 12841 atatgctacc cgtttagggt taacaagggg gccttataaa cactattgct aatgccctct 12901 tgagggtccg cttatcggta gctacacagg cccctctgat tgacgttggt gtagcctccc 12961 gtagtcttcc tgggcccctg ggaggtacat gtcccccagc attggtgtaa gagcttcagc 13021 caagagttac acataaaggc aatgttgtgt tgcagtccac agactgcaaa gtctgctcca 13081 ggatgaaagc cactcagtgt tggcaaatgt gcacatccat ttataaggat gtcaactaca 13141 gtcagagaac ccctttgtgt ttggtccccc cccgtgtcac atgtggaaca gggcccagtt 13201 ggcaagttgt accaaccaac tgaagggatt acatgcactg ccccgaatac aaaacaaaag 13261 cgctcctcgt accagcgaag aaggggcaga gatgccgtag tcaggtttag ttcgtccggc 13321 ggcggggc //