出品公司: | Invitrogen |
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载体名称: | pEBVHisC |
质粒类型: | 哺乳动物表达载体;附加体载体 |
高拷贝/低拷贝: | 高拷贝 |
克隆方法: | 限制性内切酶,多克隆位点 |
启动子: | RSV |
载体大小: | 10029 bp |
5' 测序引物及序列: | pREP Forward 5’ GCTCGATACAATAAACGCC 3’ |
3' 测序引物及序列: | SV40pA rev 5'GAAATTTGTGATGCTATTGC' |
载体标签: | His tag (N-term) |
载体抗性: | 氨苄青霉素 |
筛选标记: | Hygromycin |
克隆菌株: | DH5α 等 |
宿主细胞(系): | 人类细胞;灵长类细胞;犬类细胞 |
备注: |
pEBVHisC载体是附加体型载体,可以在基因组外自主复制,不需要整合和转座到基因组上,无需包装成病毒,即可实现稳定表达。其它附加体载体有: pREP4 PCEP4 pREP7 pREP10 pEBNA-DEST pEBVHisA pEBVHisB pEBVHisC pREP4/CAT pEBVHis-lacZ |
产品目录号: | -- |
稳定性: | 稳表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 非病毒 |
买家导航
pEBVHisC载体质粒基本信息
pEBVHisC质粒图谱载体图谱和pEBVHisC载体序列质粒序列多克隆位点信息
pEBVHisC质粒载体简介
pEBVHisC质粒序列载体序列
LOCUS pEBVHisC 10315 bp DNA SYN DEFINITION pEBVHisC ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..10315 /organism="pEBVHisC" /mol_type="other DNA" misc_feature 112..134 /label="pGEX_3_primer" terminator 279..410 /label="SV40_PA_terminator" misc_feature 367..386 /label="EBV_rev_primer" misc_feature complement(506..529) /label="Xpress_EK" misc_feature complement(533..565) /label="T7_leader" misc_feature complement(548..566) /label="Xpress_fwd_primer" misc_feature complement(632..650) /label="pREP_fwd_primer" CDS 1211..1948 /label="ORF frame 2" /translation="MRIGRPLGARNPRGGFWGSGVFGSHRRPVFVSRQYMRSMPRPSK NHGSVCSVQSWTRPHATPKIITPTNHKPFPMGDPVPNPRGQWLWQGLPPRRWLRALGL HPNLGGGVGKRKKRGRIGPNGVSVGYRQSASPGTEPRVYEQTTQHPCVLFCLFIAVIA RVPSGIVSFRVSVSLPHLPYSFALGRVLGRRFPLSASTSTQPSVQTAALLRAICVRPT VPAPDRTIASHRPCAQAASSKLPSTKL*" terminator complement(1469..1739) /label="TK_PA_terminator" /translation="MRIGRPLGARNPRGGFWGSGVFGSHRRPVFVSRQYMRSMPRPSK NHGSVCSVQSWTRPHATPKIITPTNHKPFPMGDPVPNPRGQWLWQGLPPRRWLRALGL HPNLGGGVGKRKKRGRIGPNGVSVGYRQSASPGTEPRVYEQTTQHPCVLFCLFIAVIA RVPSGIVSFRVSVSLPHLPYSFALGRVLGRRFPLSASTSTQPSVQTAALLRAICVRPT VPAPDRTIASHRPCAQAASSKLPSTKL*" CDS complement(1747..2772) /label="ORF frame 2" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS TRPRAKE*" gene complement(1750..2757) /label="hygroB" /gene="hygroB" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS TRPRAKE*" CDS 2014..3384 /label="ORF frame 1" /translation="MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT AGDAIGQALAEFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLFHISLPGICGTLLTLLSGSLQGRSV FEATRVTLICEVDLGPRRPDCICVFEFANDKTLGGVCVIIELKTCKYISSGDTASKRE QRATGMKQGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDH QGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAW SRATST*" promoter 2891..3039 /label="TATA_promoter" /translation="MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT AGDAIGQALAEFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLFHISLPGICGTLLTLLSGSLQGRSV FEATRVTLICEVDLGPRRPDCICVFEFANDKTLGGVCVIIELKTCKYISSGDTASKRE QRATGMKQGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDH QGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAW SRATST*" gene 3046..3384 /label="tet (852 - 1190)" /gene="tet (852 - 1190)" /translation="MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT AGDAIGQALAEFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLFHISLPGICGTLLTLLSGSLQGRSV FEATRVTLICEVDLGPRRPDCICVFEFANDKTLGGVCVIIELKTCKYISSGDTASKRE QRATGMKQGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDH QGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAW SRATST*" CDS 3288..3899 /label="ORF frame 3" /translation="MERVGMDCRRRPIPCLPPRVASRCMEPGHLDLNGSRRHLANGFT TPRIGANQFLRRTVNAQTNPWQNISIASAISSSRTRRSKRPGTVKRPRCWRFSIGSAP LTSITKIDAQVRGGETRQDYKDTRRFPLEAPSCALLFRPCRLPDTCPPFSLREAWRFL IAHAVGISVRCRSFAPSWAVCTNPPFSPTAAPYPVTIVLSPTR*" gene complement(4333..5193) /label="Ampicillin" /gene="Ampicillin" /translation="MERVGMDCRRRPIPCLPPRVASRCMEPGHLDLNGSRRHLANGFT TPRIGANQFLRRTVNAQTNPWQNISIASAISSSRTRRSKRPGTVKRPRCWRFSIGSAP LTSITKIDAQVRGGETRQDYKDTRRFPLEAPSCALLFRPCRLPDTCPPFSLREAWRFL IAHAVGISVRCRSFAPSWAVCTNPPFSPTAAPYPVTIVLSPTR*" CDS complement(4333..5193) /label="ORF frame 2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" promoter complement(5235..5263) /label="AmpR_promoter" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" CDS complement(5819..7033) /label="ORF frame 1" /translation="MAFYLEGACAVETRMMMTDYWDSWASFLHVHDLSPWLFHDFPPW LFHVLYPGGLHYLLDPGLHYLLDPGLHCLLDPGLHLLLLPLLLLPLLLLLPLLPLLLL PLLPLLLLPLLPLLLLPLLPLLLLLPLLPLLLLLPLLPLLLLPLLPLLLLPLLPLLLL PLLLLPLLLLPLLLLPLLPLLPLLLLLPLLLLPLLPLLPLLLLPLLLLLPLLPLLPLL LLLPLLPLLLLLPLLLLLPLLPLLPLLLLLPLLPLLLLLPLLLLLPLLPLLPLLLLLP LLLLLPLLPLLPLLPLLLLLPLLLLLPLLLLPLPLLLLLLFHRGSLCSQCNLDVFGVS GHHLYVLALILSRPGLLVFRLLVLVLFPVLVHGYHPLFFEVHCRRSLLVQMCLPSLLG HFQVLYLAPRQT*" CDS 5821..7746 /label="ORF frame 1" /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRG RGRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGG AGAGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAG GGAGGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGA GAGGGAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAG GAGAGGGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGS GGRGRGGSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSG SPPRRPPPGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGP RGQGDGGRRKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVF VYGGSKTSLYNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFL QTHIFAEVLKDAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDG DDGDEGGDGDEGEEGQE*" CDS 5936..7048 /label="ORF frame 2" /translation="MDEDGEEDEDEEAEDQEPRAAQDQGQDIEMVSGDPKNVQVALAA KGPTVEQEQEQEREGQEQEGQEQEEGQEQEEGQEGQEGQEGQEQEEGQEQEEGQEGQE GQEQEEGQEQEEGQEGQEQEEGQEGQEGQEQEEGQEQEEGQEGQEQEEGQEGQEGQEQ EEGQEQEGQEGQEGQEQEGQEQEEGQEGQEGQEQEGQEQEGQEQEGQEQEGQEGQEQE GQEGQEQEGQEGQEQEEGQEGQEQEEGQEGQEQEGQEGQEQEGQEGQEQEGQEGQEQE EGQEQEGQEQEVEAGVEEAVEAGVEEVVEAGVEEVVEAAGVEDVKEPGGEVVKEPGGE VVDVEKRGPGVPVVSHHHPGLHRAGPLQVEGHFSTL*" rep_origin 8050..10220 /label="EBV_origin" /translation="MDEDGEEDEDEEAEDQEPRAAQDQGQDIEMVSGDPKNVQVALAA KGPTVEQEQEQEREGQEQEGQEQEEGQEQEEGQEGQEGQEGQEQEEGQEQEEGQEGQE GQEQEEGQEQEEGQEGQEQEEGQEGQEGQEQEEGQEQEEGQEGQEQEEGQEGQEGQEQ EEGQEQEGQEGQEGQEQEGQEQEEGQEGQEGQEQEGQEQEGQEQEGQEQEGQEGQEQE GQEGQEQEGQEGQEQEEGQEGQEQEEGQEGQEQEGQEGQEQEGQEGQEQEGQEGQEQE EGQEQEGQEQEVEAGVEEAVEAGVEEVVEAGVEEVVEAAGVEDVKEPGGEVVKEPGGE VVDVEKRGPGVPVVSHHHPGLHRAGPLQVEGHFSTL*" ORIGIN 1 tctagagtcg accggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct 61 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct 121 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagccg gatcataatc 181 agccatacca catttgtaga ggttttactt gctttaaaaa acctccccac ctccccctga 241 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg 301 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt 361 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc gatccggcct 421 tgccggcctc gatcgagcgg ccgctagcaa gcttcgaatt cccatatggt accagctgca 481 gatctcgagg tcggatccat cgatccttat cgtcatcgtc gtacagatcc cgacccattt 541 gctgtccacc agtcatgcta gccataccat gatgatgatg atgatgagaa ccccccatgg 601 aggtgcacac caatgtggtg aatggtcaaa tggcgtttat tgtatcgagc taggcactta 661 aatacaatta tctctgcaat gcggaattca gtggttcgtc caatccatgt cagacctgtc 721 tgttgccttc ctaataaggc acgatcgtac caccttactt ccaccaatcg gcatgcacgg 781 tgctttttct ctccttgtaa ggcatgttgc taactcatcg ttaccatgtt gcaagactac 841 aagtgtattg cataagacta catttccccc tccctatgca aaagcgaaac tactatatcc 901 tgaggggact cctaaccgcg tacaaccgaa gccccgctgg gcgcctaaac acaccctagt 961 cccctcagat acgcgtatat ctggcccgta catcgcgaag cagcgcaaaa cgcctaaccc 1021 taagcagatt cttcatgcaa ttgtcggtca agccttgcct tgttgtagct taaattttgc 1081 tcgcgcacta ctcagcgacc tccaacacac aagcagggag cagatactgg cttaactatg 1141 cggcagcaga gcagattgta ctgagagtgc accatacaag ctcaggatct gcgatgataa 1201 gctgtcaaac atgagaattg gtcgaccact gggcgccaga aatccgcgcg gtggtttttg 1261 ggggtcgggg gtgtttggca gccacagacg cccggtgttc gtgtcgcgcc agtacatgcg 1321 gtccatgccc aggccatcca aaaaccatgg gtctgtctgc tcagtccagt cgtggaccag 1381 accccacgca acgcccaaaa taataacccc cacgaaccat aaaccattcc ccatggggga 1441 ccccgtccct aacccacggg gccagtggct atggcagggc ctgccgcccc gacgttggct 1501 gcgagccctg ggccttcacc cgaacttggg gggtggggtg gggaaaagga agaaacgcgg 1561 gcgtattggc cccaatgggg tctcggtggg gtatcgacag agtgccagcc ctgggaccga 1621 accccgcgtt tatgaacaaa cgacccaaca cccgtgcgtt ttattctgtc tttttattgc 1681 cgtcatagcg cgggttcctt ccggtattgt ctccttccgt gtttcagtta gcctccccca 1741 tctcccctat tcctttgccc tcggacgagt gctggggcgt cggtttccac tatcggcgag 1801 tacttctaca cagccatcgg tccagacggc cgcgcttctg cgggcgattt gtgtacgccc 1861 gacagtcccg gctccggatc ggacgattgc gtcgcatcga ccctgcgccc aagctgcatc 1921 atcgaaattg ccgtcaacca agctctgata gagttggtca agaccaatgc ggagcatata 1981 cgcccggagc cgcggcgatc ctgcaagctc cggatgcctc cgctcgaagt agcgcgtctg 2041 ctgctccata caagccaacc acggcctcca gaagaagatg ttggcgacct cgtattggga 2101 atccccgaac atcgcctcgc tccagtcaat gaccgctgtt atgcggccat tgtccgtcag 2161 gacattgttg gagccgaaat ccgcgtgcac gaggtgccgg acttcggggc agtcctcggc 2221 ccaaagcatc agctcatcga gagcctgcgc gacggacgca ctgacggtgt cgtccatcac 2281 agtttgccag tgatacacat ggggatcagc aatcgcgcat atgaaatcac gccatgtagt 2341 gtattgaccg attccttgcg gtccgaatgg gccgaacccg ctcgtctggc taagatcggc 2401 cgcagcgatc gcatccatgg cctccgcgac cggctgcaga acagcgggca gttcggtttc 2461 aggcaggtct tgcaacgtga caccctgtgc acggcgggag atgcaatagg tcaggctctc 2521 gctgaattcc ccaatgtcaa gcacttccgg aatcgggagc gcggccgatg caaagtgccg 2581 ataaacataa cgatctttgt agaaaccatc ggcgcagcta tttacccgca ggacatatcc 2641 acgccctcct acatcgaagc tgaaagcacg agattcttcg ccctccgaga gctgcatcag 2701 gtcggagacg ctgtcgaact tttcgatcag aaacttctcg acagacgtcg cggtgagttc 2761 aggctttttc atatctcatt gcccgggatc tgcggcacgc tgttgacgct gttaagcggg 2821 tcgctgcagg gtcgctcggt gttcgaggcc acacgcgtca ccttaatatg cgaagtggac 2881 ctgggaccgc gccgccccga ctgcatctgc gtgttcgaat tcgccaatga caagacgctg 2941 ggcggggttt gtgtcatcat agaactaaag acatgcaaat atatttcttc cggggacacc 3001 gccagcaaac gcgagcaacg ggccacgggg atgaagcagg gcatggcggc cgacgcgctg 3061 ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg ccttccccat tatgattctt 3121 ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat 3181 gacgaccatc agggacagct tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc 3241 actggaccgc tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg gaacgggttg 3301 gcatggattg taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca 3361 tggagccggg ccacctcgac ctgaatggaa gccggcggca cctcgctaac ggattcacca 3421 ctccaagaat tggagccaat caattcttgc ggagaactgt gaatgcgcaa accaaccctt 3481 ggcagaacat atccatcgcg tccgccatct ccagcagccg cacgcggcgc agcaaaaggc 3541 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 3601 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 3661 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 3721 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 3781 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 3841 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 3901 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 3961 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt 4021 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 4081 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 4141 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 4201 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 4261 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 4321 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 4381 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 4441 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 4501 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 4561 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 4621 tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg 4681 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 4741 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 4801 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 4861 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 4921 gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac 4981 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 5041 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 5101 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 5161 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 5221 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 5281 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat 5341 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga 5401 attctcatgt ttgacagctt atcatcgcga taagctgatc ctcacaggcc gcacccagct 5461 tttcttccgt tgccccagta gcatctctgt ctggtgacct tgaagaggaa gaggaggggt 5521 cccgagaatc cccatcccta ccgtccagca aaaaggggga cgaggaattt gaggcctggc 5581 ttgaggctca ggacgcaaat cttgaggatg ttcagcggga gttttccggg ctgcgagtaa 5641 ttggtgatga ggacgaggat ggttcggagg atggggaatt ttcagacctg gatctgtctg 5701 acagcgacca tgaaggggat gagggtgggg gggctgttgg agggggcagg agtctgcact 5761 ccctgtattc actgagcgtc gtctaataaa gatgtctatt gatctctttt agtgtgaatc 5821 atgtctgacg aggggccagg tacaggacct ggaaatggcc taggagagaa gggagacaca 5881 tctggaccag aaggctccgg cggcagtgga cctcaaagaa gagggggtga taaccatgga 5941 cgaggacggg gaagaggacg aggacgagga ggcggaagac caggagcccc gggcggctca 6001 ggatcagggc caagacatag agatggtgtc cggagacccc aaaaacgtcc aagttgcatt 6061 ggctgcaaag ggacccacgg tggaacagga gcaggagcag gagcgggagg ggcaggagca 6121 ggaggggcag gagcaggagg aggggcagga gcaggaggag gggcaggagg ggcaggaggg 6181 gcaggagggg caggagcagg aggaggggca ggagcaggag gaggggcagg aggggcagga 6241 ggggcaggag caggaggagg ggcaggagca ggaggagggg caggaggggc aggagcagga 6301 ggaggggcag gaggggcagg aggggcagga gcaggaggag gggcaggagc aggaggaggg 6361 gcaggagggg caggagcagg aggaggggca ggaggggcag gaggggcagg agcaggagga 6421 ggggcaggag caggaggggc aggaggggca ggaggggcag gagcaggagg ggcaggagca 6481 ggaggagggg caggaggggc aggaggggca ggagcaggag gggcaggagc aggaggggca 6541 ggagcaggag gggcaggagc aggaggggca ggaggggcag gagcaggagg ggcaggaggg 6601 gcaggagcag gaggggcagg aggggcagga gcaggaggag gggcaggagg ggcaggagca 6661 ggaggagggg caggaggggc aggagcagga ggggcaggag gggcaggagc aggaggggca 6721 ggaggggcag gagcaggagg ggcaggaggg gcaggagcag gaggaggggc aggagcagga 6781 ggggcaggag caggaggtgg aggccggggt cgaggaggca gtggaggccg gggtcgagga 6841 ggtagtggag gccggggtcg aggaggtagt ggaggccgcc ggggtagagg acgtgaaaga 6901 gccagggggg gaagtcgtga aagagccagg gggagaggtc gtggacgtgg agaaaagagg 6961 cccaggagtc ccagtagtca gtcatcatca tccgggtctc caccgcgcag gccccctcca 7021 ggtagaaggc catttttcca ccctgtaggg gaagccgatt attttgaata ccaccaagaa 7081 ggtggcccag atggtgagcc tgacgtgccc ccgggagcga tagagcaggg ccccgcagat 7141 gacccaggag aaggcccaag cactggaccc cggggtcagg gtgatggagg caggcgcaaa 7201 aaaggagggt ggtttggaaa gcatcgtggt caaggaggtt ccaacccgaa atttgagaac 7261 attgcagaag gtttaagagc tctcctggct aggagtcacg tagaaaggac taccgacgaa 7321 ggaacttggg tcgccggtgt gttcgtatat ggaggtagta agacctccct ttacaaccta 7381 aggcgaggaa ctgcccttgc tattccacaa tgtcgtctta caccattgag tcgtctcccc 7441 tttggaatgg cccctggacc cggcccacaa cctggcccgc taagggagtc cattgtctgt 7501 tatttcatgg tctttttaca aactcatata tttgctgagg ttttgaagga tgcgattaag 7561 gaccttgtta tgacaaagcc cgctcctacc tgcaatatca gggtgactgt gtgcagcttt 7621 gacgatggag tagatttgcc tccctggttt ccacctatgg tggaaggggc tgccgcggag 7681 ggtgatgacg gagatgacgg agatgaagga ggtgatggag atgagggtga ggaagggcag 7741 gagtgatgta acttgttagg agacgccctc aatcgtatta aaagccgtgt attcccccgc 7801 actaaagaat aaatccccag tagacatcat gcgtgctgtt ggtgtatttc tggccatctg 7861 tcttgtcacc attttcgtcc tcccaacatg gggcaattgg gcatacccat gttgtcacgt 7921 cactcagctc cgcgctcaac accttctcgc gttggaaaac attagcgaca tttacctggt 7981 gagcaatcag acatgcgacg gctttagcct ggcctcctta aattcaccta agaatgggag 8041 caaccagcat gcaggaaaag gacaagcagc gaaaattcac gcccccttgg gaggtggcgg 8101 catatgcaaa ggatagcact cccactctac tactgggtat catatgctga ctgtatatgc 8161 atgaggatag catatgctac ccggatacag attaggatag catatactac ccagatatag 8221 attaggatag catatgctac ccagatatag attaggatag cctatgctac ccagatataa 8281 attaggatag catatactac ccagatatag attaggatag catatgctac ccagatatag 8341 attaggatag cctatgctac ccagatatag attaggatag catatgctac ccagatatag 8401 attaggatag catatgctat ccagatattt gggtagtata tgctacccag atataaatta 8461 ggatagcata tactacccta atctctatta ggatagcata tgctacccgg atacagatta 8521 ggatagcata tactacccag atatagatta ggatagcata tgctacccag atatagatta 8581 ggatagccta tgctacccag atataaatta ggatagcata tactacccag atatagatta 8641 ggatagcata tgctacccag atatagatta ggatagccta tgctacccag atatagatta 8701 ggatagcata tgctatccag atatttgggt agtatatgct acccatggca acattagccc 8761 accgtgctct cagcgacctc gtgaatatga ggaccaacaa ccctgtgctt ggcgctcagg 8821 cgcaagtgtg tgtaatttgt cctccagatc gcagcaatcg cgcccctatc ttggcccgcc 8881 cacctactta tgcaggtatt ccccggggtg ccattagtgg ttttgtgggc aagtggtttg 8941 accgcagtgg ttagcggggt tacaatcagc caagttatta cacccttatt ttacagtcca 9001 aaaccgcagg gcggcgtgtg ggggctgacg cgtgccccca ctccacaatt tcaaaaaaaa 9061 gagtggccac ttgtctttgt ttatgggccc cattggcgtg gagccccgtt taattttcgg 9121 gggtgttaga gacaaccagt ggagtccgct gctgtcggcg tccactctct ttccccttgt 9181 tacaaataga gtgtaacaac atggttcacc tgtcttggtc cctgcctggg acacatctta 9241 ataaccccag tatcatattg cactaggatt atgtgttgcc catagccata aattcgtgtg 9301 agatggacat ccagtcttta cggcttgtcc ccaccccatg gatttctatt gttaaagata 9361 ttcagaatgt ttcattccta cactagtatt tattgcccaa ggggtttgtg agggttatat 9421 tggtgtcata gcacaatgcc accactgaac cccccgtcca aattttattc tgggggcgtc 9481 acctgaaacc ttgttttcga gcacctcaca tacaccttac tgttcacaac tcagcagtta 9541 ttctattagc taaacgaagg agaatgaaga agcaggcgaa gattcaggag agttcactgc 9601 ccgctccttg atcttcagcc actgcccttg tgactaaaat ggttcactac cctcgtggaa 9661 tcctgacccc atgtaaataa aaccgtgaca gctcatgggg tgggagatat cgctgttcct 9721 taggaccctt ttactaaccc taattcgata gcatatgctt cccgttgggt aacatatgct 9781 attgaattag ggttagtctg gatagtatat actactaccc gggaagcata tgctacccgt 9841 ttagggttaa caagggggcc ttataaacac tattgctaat gccctcttga gggtccgctt 9901 atcggtagct acacaggccc ctctgattga cgttggtgta gcctcccgta gtcttcctgg 9961 gcccctggga ggtacatgtc ccccagcatt ggtgtaagag cttcagccaa gagttacaca 10021 taaaggcaat gttgtgttgc agtccacaga ctgcaaagtc tgctccagga tgaaagccac 10081 tcagtgttgg caaatgtgca catccattta taaggatgtc aactacagtc agagaacccc 10141 tttgtgtttg gtcccccccc gtgtcacatg tggaacaggg cccagttggc aagttgtacc 10201 aaccaactga agggattaca tgcactgccc cgaatacaaa acaaaagcgc tcctcgtacc 10261 agcgaagaag gggcagagat gccgtagtca ggtttagttc gtccggcggc ggggc //
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pEBVHisC质粒载体应用举例
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