出品公司: | Invitrogen |
---|---|
载体名称: | pHOOK-3 |
质粒类型: | 哺乳动物表达载体;Capture-Tec系统;免疫磁珠 |
高拷贝/低拷贝: | 高拷贝 |
克隆方法: | 限制性内切酶,多克隆位点 |
启动子: | CMV |
载体大小: | 6199 bp |
5' 测序引物及序列: | CMV fwd 5’ CGCAAATGGGCGGTAGGCGTG3’ |
3' 测序引物及序列: | SV40pA rev 5'GAAATTTGTGATGCTATTGC' |
载体标签: | IgK、HA tag (N-term);2XMyc tag (N-term) |
载体抗性: | 氨苄青霉素 |
筛选标记: | 博来霉素 |
克隆菌株: | DH5α 等 |
宿主细胞(系): | 哺乳动物细胞 |
备注: |
pHOOK3载体可以用于: 1. The effect of dominant, negative mutations of growth regulating proteins or toxic gene products 2. The acute effects of constitutive mutations 3. The effect of anti-sense RNA or ribozymes 4. Temporal events along a developmental or signaling pathway 5. Activation or inactivation of downstream genes using differential display 6. You may also use the pHook-3 System to isolate a homogeneous population of transfected cells for: a) Immunoblot analysis of proteins b) Isolation of DNA or RNA c) Flow Cytometry See pHOOK-1 |
产品目录号: | V690-20 |
稳定性: | 稳表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 非病毒 |
买家导航
pHook-3载体质粒基本信息
pHook-3质粒图谱载体图谱和pHook-3载体序列质粒序列多克隆位点信息
pHook-3质粒载体简介
pHook-3载体使用方法——免疫磁珠法
pHook-3质粒序列载体序列
LOCUS Exported 6199 bp ds-DNA circular SYN 13-AUG-2016 KEYWORDS pHOOK-3 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6199) AUTHORS Biofeng Lab. TITLE Direct Submission FEATURES Location/Qualifiers source 1..6199 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 29..358 /note="SV40 promoter" /note="SV40 enhancer and early promoter" rep_origin 209..344 /note="SV40 ori" /note="SV40 origin of replication" promoter 787..834 /note="EM7 promoter" /note="synthetic bacterial promoter " promoter 826..844 /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" CDS 854..1228 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /note="BleoR" /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 1292..1426 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" rep_origin complement(1590..2178) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" enhancer 2263..2566 /note="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" promoter 2567..2770 /note="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" promoter 2905..2923 /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" polyA_signal 3261..3309 /note="HSV TK poly(A) signal" /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" promoter 4079..4341 /note="RSV promoter" /note="Rous sarcoma virus enhancer/promoter" CDS 4366..4428 /codon_start=1 /product="leader sequence from mouse immunoglobulin kappa light chain" /note="Ig-kappa leader" /translation="METDTLLLWVLLLWVPGSTGD" CDS 4429..4455 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /note="HA" /translation="YPYDVPDYA" CDS 5194..5223 /codon_start=1 /product="Myc (human c-Myc oncogene) epitope tag" /note="Myc" /translation="EQKLISEEDL" CDS 5239..5268 /codon_start=1 /product="Myc (human c-Myc oncogene) epitope tag" /note="Myc" /translation="EQKLISEEDL" promoter complement(5460..5478) /note="SP6 promoter" /note="promoter for bacteriophage SP6 RNA polymerase" polyA_signal 5504..5728 /note="bGH poly(A) signal" /note="bovine growth hormone polyadenylation signal" rep_origin 5774..3 /direction=RIGHT /note="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" ORIGIN 1 attatggtga ccggatccgc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg 61 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg 121 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc 181 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca 241 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc 301 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa 361 gctggctcga tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg 421 tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg 481 taagtttaga gctcaggtcg agaccgggcc tttgtccggc gctcccttgg agcctaccta 541 gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac gtctttgttt 601 cgttttctgt tctgcgccgt tacaacttca aggtatgcgc tgggacctgg caggcggcat 661 ctgggacccc taggaagggc ttgggggtcc tcgtgcccaa ggcagggaac atagtggtcc 721 caggaagggg agcagaggca tcagggtgtc cactttgtct ccgcagctcc tgagcctgca 781 gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata cgactcacta 841 taggagggcc accatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt 901 cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga 961 cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca 1021 ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc 1081 cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga 1141 gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt 1201 gcacttcgtg gccgaggagc aggactgacc gacgccgacc aacaccgccg gtccgacggc 1261 ggcccacggg tcccaggggg gtcgacctcg aaacttgttt attgcagctt ataatggtta 1321 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag 1381 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatccctc ggagatcgat 1441 ctgggcccat gcggccggcc gcggatcgat gctcactcaa aggcggtaat acggttatcc 1501 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg 1561 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 1621 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 1681 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 1741 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg 1801 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 1861 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 1921 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 1981 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt 2041 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 2101 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 2161 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 2221 aacgaaaact cacgttaagg gattttggtc atggctagag tccgttacat aacttacggt 2281 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta 2341 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg 2401 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga 2461 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt 2521 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg 2581 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc 2641 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg 2701 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat 2761 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga 2821 cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac 2881 ggacctgcag cacgtgttga caattaatac gactcactat agggagaccc aagcttggta 2941 ccagatcttt taaagctagc tctagagtac cgagctcgga tcccccgggc cagtgtgctg 3001 gaagtaccga gctcgatcgt acccagcttg gaggttgcag ggtcgatgcg acgcaatcgt 3061 ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg 3121 gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc 3181 gagggcaaag gaatagggga gatgggggag gctaactgaa acacggaagg agacaatacc 3241 ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg ggtgttgggt 3301 cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga 3361 gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt 3421 cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc atagccactg 3481 gccccgtggg ttagggacgg ggtcccccat ggggaatggt ttatggttcg tgggggttat 3541 tattttgggc gttgcgtggg gtctggtcca cgactggact gagcagacag acccatggtt 3601 tttggatggc ctgggcatgg accgcatgta ctggcgcgac acgaacaccg ggcgtctgtg 3661 gctgccaaac acccccgacc cccaaaaacc accgcgcgga tttctggcgc ccagtggtcg 3721 accaattctc atgtttgaca gcttatcatc gcagatcctg agcttgtatg gtgcactctc 3781 agtacaatct gctctgctgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg 3841 gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac 3901 aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc 3961 agatatacgc gtatctgagg ggactagggt gtgtttaggc gcccagcggg gcttcggttg 4021 tacgcggtta ggagtcccct caggatatag tagtttcgct tttgcatagg gagggggaaa 4081 tgtagtctta tgcaatacac ttgtagtctt gcaacatggt aacgatgagt tagcaacatg 4141 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc 4201 gtgccttatt aggaaggcaa cagacaggtc tgacatggat tggacgaacc actgaattcc 4261 gcattgcaga gataattgta tttaagtgcc tagctcgata caataaacgc catttgacca 4321 ttcaccacat tggtgtgcac ctccaagctg ggtacgatat ccaccatgga gacagacaca 4381 ctcctgctat gggtactgct gctctgggtt ccaggttcca ctggtgacta tccatatgat 4441 gttccagatt atgctggggc ccagccggcc atggccgagg tcaagctgca ggagtcaggg 4501 ggaggcttag tgcagcctgg agggtcccgg aaactctcct gtgcagcctc tggattcact 4561 ttcagtagct ttggaatgca ctgggttcgt caggctccag agaaggggct ggagtgggtc 4621 gcatatatta gtagtggcag tagtaccatc tactatgcag acacagtgaa gggacgattc 4681 accatctcca gagacaatcc caagaacacc ctgttcctgc aaatgaccag tctaaggtct 4741 gaggacacgg ncatgtatta ctgtgcaaga gattacgggg cttattgggg ccaagggacc 4801 acggncaccg tctcctcagg tggaggcggc tcaggcggag gtggctctgg cggtggcgga 4861 tcggacattg agctcaccca gtctccagca atcatgtctg catctccagg ggagagggtc 4921 accatgacct gcagtgccag ttcaagtgta aggtacatga actggttcca acagaagtca 4981 ggcacctccc ccaaaagatg gatttatgac acatccaaac tgtcttctgg agtccctgct 5041 cgcttcagtg gcagtgggtc tgggacctct tactctctca caatcagcag catggaggct 5101 gaagatgctg ccacttacta ctgccagcag tggagtagta acccactcac gttcggtgct 5161 gggaccaagc tggagctgaa acgggcggcc gcagaacaaa aactcatctc agaagaggat 5221 ctgaatgggg ccgtcgacga acaaaaactc atctcagaag aggatctgaa tgctgtgggc 5281 caggacacgc aggaggtcat cgtggtgcca cactccttgc cctttaaggt ggtggtgatc 5341 tcagccatcc tggccctggt ggtgctcacc atcatctccc ttatcatcct catcatgctt 5401 tggcagaaga agccacgtta ggcggccgct cgagcatgca tctagctaga gggccctatt 5461 ctatagtgtc acctaaatgc tagagctcgc tgatcagcct cgactgtgcc ttctagttgc 5521 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc 5581 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct 5641 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg 5701 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct 5761 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg 5821 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct 5881 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg catcccttta 5941 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt 6001 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg 6061 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat 6121 tcttttgatt tataagggat tttggggatt tcggcctatt ggttaaaaaa tgagctgatt 6181 taacaaaaat ttaacgcga //
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pHook-3质粒载体应用举例
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