| 出品公司: | Clontech |
|---|---|
| 载体名称: | pAP1(PMA)-TA-Luc |
| 质粒类型: | 信号通路报告系统载体 |
| 高拷贝/低拷贝: | -- |
| 启动子: | -- |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 4.9 kb |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | Ampicillin |
| 筛选标记: | -- |
| 备注: | -- |
| 产品目录号: | 631906 |
| 稳定性: | -- |
| 组成型: | -- |
| 病毒/非病毒: | -- |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
-TA-Luc载体图谱.jpg)
-TA-Luc载体特征.jpg)
载体简介
pAP1(PMA)-TA-Luc is a member of the signal transduction reporter vectors. It is designed for
monitoring the induction of the protein kinase C (PKC) signal transduction pathway, as well as related
pathways such as the MAPK pathway. pAP1(PMA)-TA-Luc contains the firefly luciferase gene (luc)
as the reporter. The vector contains multiple copies of the activator protein 1 (AP1) enhancer, located
upstream of luc, that responds specifically to phorbol ester treatment (1). AP1(PMA) is fused to a
minimal TA promoter, the TATA box from the Herpes simplex virus thymidine kinase (HSV-TK) promoter.
The luciferase coding sequence is followed by the SV40 late polyadenylation signal to ensure proper,
efficient processing of the luciferase transcript in eukaryotic cells. A synthetic transcription blocker (TB)
is located upstream of AP1(PMA), which is composed of adjacent polyadenylation and transcription
pause sites for blocking nonspecific transcription (2). The vector backbone also contains an f1 origin
for single-stranded DNA production, a pUC origin of replication, and an ampicillin resistance gene
for propagation and selection in E. coli.
