出品公司: | Addgene |
---|---|
载体名称: | pBABE-HAII |
质粒类型: | 逆病毒载体 |
高拷贝/低拷贝: | 高拷贝 |
克隆方法: | 限制性内切酶,多克隆位点 |
启动子: | MSCV |
载体大小: | 5206 bp |
5' 测序引物及序列: | pBABE 5' primer 5'CTTTATCCAGCCCTCAC3' |
3' 测序引物及序列: | pBABE 3' primer 5'ACCCTAACTGACACACATTCC3' |
载体标签: | 无 |
载体抗性: | 氨苄青霉素 |
筛选标记: | Puromycin |
克隆菌株: | DH5α、HB101等 |
宿主细胞(系): | 常规哺乳动物细胞系 |
备注: |
推荐使用逆病毒包装载体:pUMVC、pCMV-VSV-G 。 |
产品目录号: | #14738 |
稳定性: | 稳表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 逆转录病毒 |
买家导航
pBABE-HAII载体质粒基本信息
pBABE-HAII质粒图谱载体图谱和pBABE-HAII载体序列质粒序列多克隆位点信息
pBABE-HAII质粒载体简介
pBABE-HAII质粒序列载体序列
LOCUS pBABE HAII 5206 bp DNA SYN DEFINITION pBABE HAII ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..5206 /organism="pBABE HAII" /mol_type="other DNA" misc_feature 12..479 /label="5_LTR2" misc_feature 18..479 /label="3MoMuLV_LTR" misc_feature 549..1350 /label="psi_plus_pack" misc_feature 1318..1334 /label="pBABE_5_primer" misc_feature complement(1445..1465) /label="pBABE_3_primer" misc_feature complement(1451..1666) /label="SV40_enhancer" promoter 1463..1731 /label="SV40_promoter" rep_origin 1630..1707 /label="SV40_origin" misc_feature 1692..1711 /label="SV40pro_F_primer" CDS complement(1765..2439) /label="ORF frame 2" /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGRAVLRALDVGGDGEAE PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG KLFAKA*" gene 1789..2388 /label="puro (variant)" /gene="puro (variant)" /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGRAVLRALDVGGDGEAE PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG KLFAKA*" CDS 1789..2388 /label="ORF frame 1" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL ETSAPRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA*" misc_feature 2487..3080 /label="3MoMuLV_LTR" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL ETSAPRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA*" misc_feature 2529..3080 /label="5_LTR2" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL ETSAPRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA*" gene complement(4218..5078) /label="Ampicillin" /gene="Ampicillin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL ETSAPRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA*" CDS complement(4218..5078) /label="ORF frame 3" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" promoter complement(5120..5148) /label="AmpR_promoter" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" ORIGIN 1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca 61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt 121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag 181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc 241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga 301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc 361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc 421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt 481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg 541 taagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta 601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa 661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc 721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg 781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt 841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt 901 ctgactgtgt ttctgtattt gtctgaaaat tagggccaga ctgttaccac tcccttaagt 961 ttgaccttag atcactggaa agatgtcgag cggctcgctc acaaccagtc ggtagatgtc 1021 aagaagagac gttgggttac cttctgctct gcagaatggc caacctttaa cgtcggatgg 1081 ccgcgagacg gcacctttaa ccgagacctc atcacccagg ttaagatcaa ggtcttttca 1141 cctggcccgc atggacaccc agaccaggtc ccctacatcg tgacctggga agccttggct 1201 tttgaccccc ctccctgggt caagcccttt gtacacccta agcctccgcc tcctcttctt 1261 ccatccgcgc cgtctctccc ccttgaacct cctctttcga ccccgcctca atcctccctt 1321 tatccagccc tcactccttc tctaggcgcc ggccggatca atggcttcaa gttatccata 1381 tgatgtgccc gactatgcct cacttggagg accctctgga tccggaattc tccgtcgacc 1441 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt 1501 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca 1561 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta 1621 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga 1681 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag 1741 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttaccat gaccgagtac 1801 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc 1861 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag 1921 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg 1981 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg 2041 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc 2101 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc 2161 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg 2221 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg 2281 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgagtgc 2341 ccgaaggacc gcgcgacctg gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac 2401 gacccgcagc gcccgaccga aaggagcgca cgaccccatg catcgataaa ataaaagatt 2461 ttatttagtc tccagaaaaa ggggggaatg aaagacccca cctgtaggtt tggcaagcta 2521 gcttaagtaa cgccattttg caaggcatgg aaaaatacat aactgagaat agagaagttc 2581 agatcaaggt caggaacaga tggaacagct gaatatgggc caaacaggat atctgtggta 2641 agcagttcct gccccggctc agggccaaga acagatggaa cagctgaata tgggccaaac 2701 aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggtccccag 2761 atgcggtcca gccctcagca gtttctagag aaccatcaga tgtttccagg gtgccccaag 2821 gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt 2881 tcgcgcgctt ctgctccccg agctcaataa aagagcccac aacccctcac tcggggcgcc 2941 agtcctccga ttgactgagt cgcccgggta cccgtgtatc caataaaccc tcttgcagtt 3001 gcatccgact tgtggtctcg ctgttccttg ggagggtctc ctctgagtga ttgactaccc 3061 gtcagcgggg gtctttcatt tccgacttgt ggtctcgctg ccttgggagg gtctcctctg 3121 agtgattgac tacccgtcag cgggggtctt cacatgcagc atgtatcaaa attaatttgg 3181 ttttttttct taagtattta cattaaatgg ccatagttgc attaatgaat cggccaacgc 3241 gcggggagag gcggtttgcg tattggcgct cttccgcttc ctcgctcact gactcgctgc 3301 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 3361 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 3421 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 3481 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 3541 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 3601 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 3661 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 3721 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 3781 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 3841 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat 3901 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 3961 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 4021 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 4081 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 4141 agatcctttt aaattaaaaa tgaagtttgc ggccgcaaat caatctaaag tatatatgag 4201 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 4261 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 4321 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 4381 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 4441 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 4501 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg 4561 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 4621 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 4681 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 4741 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 4801 atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc 4861 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 4921 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 4981 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 5041 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 5101 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 5161 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccac //
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pBABE-HAII质粒载体应用举例
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