出品人: | Bob Weinberg |
---|---|
载体名称: | pBABE-hygro-hTERT |
质粒类型: | 逆病毒载体 |
高拷贝/低拷贝: | 高拷贝 |
克隆方法: | 限制性内切酶,多克隆位点 |
启动子: | MSCV |
载体大小: | 8703 bp |
5' 测序引物及序列: | pBABE 5' primer 5'CTTTATCCAGCCCTCAC3' |
3' 测序引物及序列: | pBABE 3' primer 5'ACCCTAACTGACACACATTCC3' |
载体标签: | 无 |
载体抗性: | 氨苄青霉素 |
筛选标记: | Hygromycin |
克隆菌株: | DH5α、HB101等 |
宿主细胞(系): | 常规哺乳动物细胞系 |
备注: |
逆病毒载体pBABE-hygro-hTERT表达telomerase reverse transcriptase,用来建永生细胞系。 推荐使用逆病毒包装载体:pUMVC、pCMV-VSV-G 。 |
产品目录号: | #1773 |
稳定性: | 稳表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 逆转录病毒 |
买家导航
pBABE-hygro-hTERT载体质粒基本信息
pBABE-hygro-hTERT质粒图谱载体图谱和pBABE-hygro-hTERT载体序列质粒序列多克隆位点信息
pBABE-hygro-hTERT质粒载体简介
pBABE-hygro-hTERT质粒序列载体序列
LOCUS pBABE-hygro-hTERT Sequencing Result 8703 bp DNA SYN DEFINITION pBABE-hygro-hTERT Sequencing Result ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..8703 /organism="pBABE-hygro-hTERT Sequencing Result" /mol_type="other DNA" misc_feature 10..477 /label="5_LTR2" misc_feature 16..477 /label="3MoMuLV_LTR" misc_feature 547..1356 /label="psi_plus_pack" misc_feature 943..1350 /label="gag" misc_feature 1286..1308 /label="MSCV_primer" misc_feature 1286..1308 /label="pLXSN_5_primer" misc_feature 1324..1340 /label="pBABE_5_primer" CDS complement(1337..2050) /label="ORF frame 3" /translation="MVPGPFASQTPSGSTSVWRGPGLSGSAELVQRRPAHLVGAAGSH QHKERAACQQVNQHVVAHAAQQQPPRPAPPQCVGHRVGQVAAHAGGEGLGGAPAGPVQ QREAEGQHVLRAALAQPLQHSGHQLLQAGHLAEGGGGGGRPCVPGHAHQALGHQRAES RRVPALHQPPALGPQAPHERGQRQHLAVVAAQQGAHGSAAGSARHRGGGRGQGFPRAQ QDEFLRTTTLGSGRRLEKE*" CDS 1436..4834 /label="ORF frame 2" /translation="MPRAPRCRAVRSLLRSHYREVLPLATFVRRLGPQGWRLVQRGDP AAFRALVAQCLVCVPWDARPPPAAPSFRQVSCLKELVARVLQRLCERGAKNVLAFGFA LLDGARGGPPEAFTTSVRSYLPNTVTDALRGSGAWGLLLRRVGDDVLVHLLARCALFV LVAPSCAYQVCGPPLYQLGAATQARPPPHASGPRRRLGCERAWNHSVREAGVPLGLPA PGARRRGGSASRSLPLPKRPRRGAAPEPERTPVGQGSWAHPGRTRGPSDRGFCVVSPA RPAEEATSLEGALSGTRHSHPSVGRQHHAGPPSTSRPPRPWDTPCPPVYAETKHFLYS SGDKEQLRPSFLLSSLRPSLTGARRLVETIFLGSRPWMPGTPRRLPRLPQRYWQMRPL FLELLGNHAQCPYGVLLKTHCPLRAAVTPAAGVCAREKPQGSVAAPEEEDTDPRRLVQ LLRQHSSPWQVYGFVRACLRRLVPPGLWGSRHNERRFLRNTKKFISLGKHAKLSLQEL TWKMSVRGCAWLRRSPGVGCVPAAEHRLREEILAKFLHWLMSVYVVELLRSFFYVTET TFQKNRLFFYRKSVWSKLQSIGIRQHLKRVQLRELSEAEVRQHREARPALLTSRLRFI PKPDGLRPIVNMDYVVGARTFRREKRAERLTSRVKALFSVLNYERARRPGLLGASVLG LDDIHRAWRTFVLRVRAQDPPPELYFVKVDVTGAYDTIPQDRLTEVIASIIKPQNTYC VRRYAVVQKAAHGHVRKAFKSHVSTLTDLQPYMRQFVAHLQETSPLRDAVVIEQSSSL NEASSGLFDVFLRFMCHHAVRIRGKSYVQCQGIPQGSILSTLLCSLCYGDMENKLFAG IRRDGLLLRLVDDFLLVTPHLTHAKTFLRTLVRGVPEYGCVVNLRKTVVNFPVEDEAL GGTAFVQMPAHGLFPWCGLLLDTRTLEVQSDYSSYARTSIRASLTFNRGFKAGRNMRR KLFGVLRLKCHSLFLDLQVNSLQTVCTNIYKILLLQAYRFHACVLQLPFHQQVWKNPT FFLRVISDTASLCYSILKAKNAGMSLGAKGAAGPLPSEAVQWLCHQAFLLKLTRHRVT YVPLLGSLRTAQTQLSRKLPGTTLTALEAAANPALPSDFKTILD*" CDS complement(2471..3691) /label="ORF frame 3" /translation="MGGLLDHGIPTHAVRVLGFDDAGDDLREPVLGDGVVRARHIHLD KVQLRRRVLGPHTQHEGAPGPVDIVQAQHRGAQEAGAPRPLVVEHAEQCLHPRGETLG PLFSAERSGSHDVVHVHNRPQPVRLGDEAESGRQQGGPGFPMLPDLCFRQLPQLHPLQ VLSDSNALQLAPDTLPVEKEPVLLKRGLRDIKERPEQLDDVHTHQPVQELGQDLLTQT VLCGRNTANPWAPAQPSAAPHAHLPRQLLQRELGMLPQGDELLGVPEEAAFVVPGAPE AWGHQPAQAGPHEAVHLPGAAVLAEQLHQATGVCVLLLGGRHRALGLLPGTDTGCWGD RSSQRAVRLEEHPVGALRVVPKQLQKQGPHLPVALGQAGQPAGSPWHPGPGTQKDGLH EPPSASQAGPQRAE*" misc_feature complement(4846..4866) /label="pBABE_3_primer" /translation="MGGLLDHGIPTHAVRVLGFDDAGDDLREPVLGDGVVRARHIHLD KVQLRRRVLGPHTQHEGAPGPVDIVQAQHRGAQEAGAPRPLVVEHAEQCLHPRGETLG PLFSAERSGSHDVVHVHNRPQPVRLGDEAESGRQQGGPGFPMLPDLCFRQLPQLHPLQ VLSDSNALQLAPDTLPVEKEPVLLKRGLRDIKERPEQLDDVHTHQPVQELGQDLLTQT VLCGRNTANPWAPAQPSAAPHAHLPRQLLQRELGMLPQGDELLGVPEEAAFVVPGAPE AWGHQPAQAGPHEAVHLPGAAVLAEQLHQATGVCVLLLGGRHRALGLLPGTDTGCWGD RSSQRAVRLEEHPVGALRVVPKQLQKQGPHLPVALGQAGQPAGSPWHPGPGTQKDGLH EPPSASQAGPQRAE*" misc_feature complement(4852..5231) /label="SV40_enhancer" /translation="MGGLLDHGIPTHAVRVLGFDDAGDDLREPVLGDGVVRARHIHLD KVQLRRRVLGPHTQHEGAPGPVDIVQAQHRGAQEAGAPRPLVVEHAEQCLHPRGETLG PLFSAERSGSHDVVHVHNRPQPVRLGDEAESGRQQGGPGFPMLPDLCFRQLPQLHPLQ VLSDSNALQLAPDTLPVEKEPVLLKRGLRDIKERPEQLDDVHTHQPVQELGQDLLTQT VLCGRNTANPWAPAQPSAAPHAHLPRQLLQRELGMLPQGDELLGVPEEAAFVVPGAPE AWGHQPAQAGPHEAVHLPGAAVLAEQLHQATGVCVLLLGGRHRALGLLPGTDTGCWGD RSSQRAVRLEEHPVGALRVVPKQLQKQGPHLPVALGQAGQPAGSPWHPGPGTQKDGLH EPPSASQAGPQRAE*" promoter 4864..5132 /label="SV40_promoter" /translation="MGGLLDHGIPTHAVRVLGFDDAGDDLREPVLGDGVVRARHIHLD KVQLRRRVLGPHTQHEGAPGPVDIVQAQHRGAQEAGAPRPLVVEHAEQCLHPRGETLG PLFSAERSGSHDVVHVHNRPQPVRLGDEAESGRQQGGPGFPMLPDLCFRQLPQLHPLQ VLSDSNALQLAPDTLPVEKEPVLLKRGLRDIKERPEQLDDVHTHQPVQELGQDLLTQT VLCGRNTANPWAPAQPSAAPHAHLPRQLLQRELGMLPQGDELLGVPEEAAFVVPGAPE AWGHQPAQAGPHEAVHLPGAAVLAEQLHQATGVCVLLLGGRHRALGLLPGTDTGCWGD RSSQRAVRLEEHPVGALRVVPKQLQKQGPHLPVALGQAGQPAGSPWHPGPGTQKDGLH EPPSASQAGPQRAE*" rep_origin 5031..5108 /label="SV40_origin" /translation="MGGLLDHGIPTHAVRVLGFDDAGDDLREPVLGDGVVRARHIHLD KVQLRRRVLGPHTQHEGAPGPVDIVQAQHRGAQEAGAPRPLVVEHAEQCLHPRGETLG PLFSAERSGSHDVVHVHNRPQPVRLGDEAESGRQQGGPGFPMLPDLCFRQLPQLHPLQ VLSDSNALQLAPDTLPVEKEPVLLKRGLRDIKERPEQLDDVHTHQPVQELGQDLLTQT VLCGRNTANPWAPAQPSAAPHAHLPRQLLQRELGMLPQGDELLGVPEEAAFVVPGAPE AWGHQPAQAGPHEAVHLPGAAVLAEQLHQATGVCVLLLGGRHRALGLLPGTDTGCWGD RSSQRAVRLEEHPVGALRVVPKQLQKQGPHLPVALGQAGQPAGSPWHPGPGTQKDGLH EPPSASQAGPQRAE*" CDS complement(5092..6012) /label="ORF frame 1" /translation="MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT AGDAIGQALAKFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLFHISLPPGIRSAGLPNLTRGRPSWQ AFCKSLGLQKSLLTTSGIAQRPRRPRPLHK*" misc_feature 5093..5112 /label="SV40pro_F_primer" /translation="MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT AGDAIGQALAKFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLFHISLPPGIRSAGLPNLTRGRPSWQ AFCKSLGLQKSLLTTSGIAQRPRRPRPLHK*" CDS 5254..6279 /label="ORF frame 1" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS TRPRAKE*" gene 5269..6276 /label="hygroB" /gene="hygroB" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS TRPRAKE*" gene complement(7661..8521) /label="Ampicillin" /gene="Ampicillin" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS TRPRAKE*" CDS complement(7661..8521) /label="ORF frame 3" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" promoter complement(8563..8591) /label="AmpR_promoter" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" misc_feature 8661..477 /label="5_LTR2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" misc_feature 8667..477 /label="3MoMuLV_LTR" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" ORIGIN 1 gcagcctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccGGCTCAGG 61 GCCAAGAACA GATGGAACAG CTGAATATGG GCCAAACAGG ATATCTGTGG TAAGCAGTTC 121 CTGCCCCGGC TCAGGGCCAA GAACAGATGG TCCCCAGATG CGGTCCAGCC CTCAGCAGTT 181 TCTAGAGAAC CATCAGATGT TTCCAGGGTG CCCCAAGGAC CTGAAATGAC CCTGTGCCTT 241 ATTTGAACTA ACCAATCAGT TCGCTTCTCG CTTCTGTTCG CGCGCTTCTG CTCCCCGAGC 301 TCAATAAAAG AGCCCACAAC CCCTCACTCG GGGCGCCAGT CCTCCGATTG ACTGAGTCGC 361 CCGGGTACCC GTGTATCCAA TAAACCCTCT TGCAGTTGCA TCCGACTTGT GGTCTCGCTG 421 TTCCTTGGGA GGGTCTCCTC TGAGTGATTG ACTACCCGTC AGCGGGGGTC TTTCATTTGG 481 GGGCTCGTCC GGGATCGGGA GACCCCTGCC CAGGGACCAC CGACCCACCA CCGGGAGGCA 541 AGCTGGCCAG CAACTTATCT GTGTCTGTCC GATTGTCTAG TGTCTATGAC TGATTTTATG 601 CGCCTGCGTC GGTACTAGTT AGCTAACTAG CTCTGTATCT GGCGGACCCG TGGTGGAACT 661 GACGAGTTCT GAACACCCGG CCGCAACCCT GGGAGACGTC CCAGGGACTT TGGGGGCCGT 721 TTTTGTGGCC CGACCTGAGG AAGGGAGTCG ATGTGGAATC CGACCCCGTC AGGATATGTG 781 GTTCTGGTAG GAGACGAGAA CCTAAAACAG TTCCCGCCTC CGTCTGAATT TTTGCTTTCG 841 GTTTGGAACC GAAGCCGCGC GTCTTGTCTG CTGCAGCGCT GCAGCATCGT TCTGTGTTGT 901 CTCTGTCTGA CTGTGTTTCT GTATTTGTCT GAAAATTAGG GCCAGACTGT TACCACTCCC 961 TTAAGTTTGA CCTTAGGTCA CTGGAAAGAT GTCGAGCGGA TCGCTCACAA CCAGTCGGTA 1021 GATGTCAAGA AGAGACGTTG GGTTACCTTC TGCTCTGCAG AATGGCCAAC CTTTAACGTC 1081 GGATGGCCGC GAGACGGCAC CTTTAACCGA GACCTCATCA CCCAGGTTAA GATCAAGGTC 1141 TTTTCACCTG GCCCGCATGG ACACCCAGAC CAGGTCCCCT ACATCGTGAC CTGGGAAGCC 1201 TTGGCTTTTG ACCCCCCTCC CTGGGTCAAG CCCTTTGTAC ACCCTAAGCC TCCGCCTCCT 1261 CTTCCTCCAT CCGCCCCGTC TCTCCCCCTT GAACCTCCTC GTTCGACCCC GCCTCGATCC 1321 TCCCTTTATC CAGCCCTCAC TCCTTCTCTA GGCGCCGGCC GGATCCCAGT GTGGTGGTAC 1381 GTAGGAATTC GTCCTGCTGC GCACGTGGGA AGCCCTGGCC CCGGCCACCC CCGCGATGCC 1441 GCGCGCTCCC CGCTGCCGAG CCGTGCGCTC CCTGCTGCGC AGCCACTACC GCGAGGTGCT 1501 GCCGCTGGCC ACGTTCGTGC GGCGCCTGGG GCCCCAGGGC TGGCGGCTGG TGCAGCGCGG 1561 GGACCCGGCG GCTTTCCGCG CGCTGGTGGC CCAGTGCCTG GTGTGCGTGC CCTGGGACGC 1621 ACGGCCGCCC CCCGCCGCCC CCTCCTTCCG CCAGGTGTCC TGCCTGAAGG AGCTGGTGGC 1681 CCGAGTGCTG CAGAGGCTGT GCGAGCGCGG CGCGAAGAAC GTGCTGGCCT TCGGCTTCGC 1741 GCTGCTGGAC GGGGCCCGCG GGGGCCCCCC CGAGGCCTTC ACCACCAGCG TGCGCAGCTA 1801 CCTGCCCAAC ACGGTGACCG ACGCACTGCG GGGGAGCGGG GCGTGGGGGC TGCTGCTGCG 1861 CCGCGTGGGC GACGACGTGC TGGTTCACCT GCTGGCACGC TGCGCGCTCT TTGTGCTGGT 1921 GGCTCCCAGC TGCGCCTACC AGGTGTGCGG GCCGCCGCTG TACCAGCTCG GCGCTGCCAC 1981 TCAGGCCCGG CCCCCGCCAC ACGCTAGTGG ACCCCGAAGG CGTCTGGGAT GCGAACGGGC 2041 CTGGAACCAT AGCGTCAGGG AGGCCGGGGT CCCCCTGGGC CTGCCAGCCC CGGGTGCGAG 2101 GAGGCGCGGG GGCAGTGCCA GCCGAAGTCT GCCGTTGCCC AAGAGGCCCA GGCGTGGCGC 2161 TGCCCCTGAG CCGGAGCGGA CGCCCGTTGG GCAGGGGTCC TGGGCCCACC CGGGCAGGAC 2221 GCGTGGACCG AGTGACCGTG GTTTCTGTGT GGTGTCACCT GCCAGACCCG CCGAAGAAGC 2281 CACCTCTTTG GAGGGTGCGC TCTCTGGCAC GCGCCACTCC CACCCATCCG TGGGCCGCCA 2341 GCACCACGCG GGCCCCCCAT CCACATCGCG GCCACCACGT CCCTGGGACA CGCCTTGTCC 2401 CCCGGTGTAC GCCGAGACCA AGCACTTCCT CTACTCCTCA GGCGACAAGG AGCAGCTGCG 2461 GCCCTCCTTC CTACTCAGCT CTCTGAGGCC CAGCCTGACT GGCGCTCGGA GGCTCGTGGA 2521 GACCATCTTT CTGGGTTCCA GGCCCTGGAT GCCAGGGACT CCCCGCAGGT TGCCCCGCCT 2581 GCCCCAGCGC TACTGGCAAA TGCGGCCCCT GTTTCTGGAG CTGCTTGGGA ACCACGCGCA 2641 GTGCCCCTAC GGGGTGCTCC TCAAGACGCA CTGCCCGCTG CGAGCTGCGG TCACCCCAGC 2701 AGCCGGTGTC TGTGCCCGGG AGAAGCCCCA GGGCTCTGTG GCGGCCCCCG AGGAGGAGGA 2761 CACAGACCCC CGTCGCCTGG TGCAGCTGCT CCGCCAGCAC AGCAGCCCCT GGCAGGTGTA 2821 CGGCTTCGTG CGGGCCTGCC TGCGCCGGCT GGTGCCCCCA GGCCTCTGGG GCTCCAGGCA 2881 CAACGAACGC CGCTTCCTCA GGAACACCAA GAAGTTCATC TCCCTGGGGA AGCATGCCAA 2941 GCTCTCGCTG CAGGAGCTGA CGTGGAAGAT GAGCGTGCGG GGCTGCGCTT GGCTGCGCAG 3001 GAGCCCAGGG GTTGGCTGTG TTCCGGCCGC AGAGCACCGT CTGCGTGAGG AGATCCTGGC 3061 CAAGTTCCTG CACTGGCTGA TGAGTGTGTA CGTCGTCGAG CTGCTCAGGT CTTTCTTTTA 3121 TGTCACGGAG ACCACGTTTC AAAAGAACAG GCTCTTTTTC TACCGGAAGA GTGTCTGGAG 3181 CAAGTTGCAA AGCATTGGAA TCAGACAGCA CTTGAAGAGG GTGCAGCTGC GGGAGCTGTC 3241 GGAAGCAGAG GTCAGGCAGC ATCGGGAAGC CAGGCCCGCC CTGCTGACGT CCAGACTCCG 3301 CTTCATCCCC AAGCCTGACG GGCTGCGGCC GATTGTGAAC ATGGACTACG TCGTGGGAGC 3361 CAGAACGTTC CGCAGAGAAA AGAGGGCCGA GCGTCTCACC TCGAGGGTGA AGGCACTGTT 3421 CAGCGTGCTC AACTACGAGC GGGCGCGGCG CCCCGGCCTC CTGGGCGCCT CTGTGCTGGG 3481 CCTGGACGAT ATCCACAGGG CCTGGCGCAC CTTCGTGCTG CGTGTGCGGG CCCAGGACCC 3541 GCCGCCTGAG CTGTACTTTG TCAAGGTGGA TGTGACGGGC GCGTACGACA CCATCCCCCA 3601 GGACAGGCTC ACGGAGGTCA TCGCCAGCAT CATCAAACCC CAGAACACGT ACTGCGTGCG 3661 TCGGTATGCC GTGGTCCAGA AGGCCGCCCA TGGGCACGTC CGCAAGGCCT TCAAGAGCCA 3721 CGTCTCTACC TTGACAGACC TCCAGCCGTA CATGCGACAG TTCGTGGCTC ACCTGCAGGA 3781 GACCAGCCCG CTGAGGGATG CCGTCGTCAT CGAGCAGAGC TCCTCCCTGA ATGAGGCCAG 3841 CAGTGGCCTC TTCGACGTCT TCCTACGCTT CATGTGCCAC CACGCCGTGC GCATCAGGGG 3901 CAAGTCCTAC GTCCAGTGCC AGGGGATCCC GCAGGGCTCC ATCCTCTCCA CGCTGCTCTG 3961 CAGCCTGTGC TACGGCGACA TGGAGAACAA GCTGTTTGCG GGGATTCGGC GGGACGGGCT 4021 GCTCCTGCGT TTGGTGGATG ATTTCTTGTT GGTGACACCT CACCTCACCC ACGCGAAAAC 4081 CTTCCTCAGG ACCCTGGTCC GAGGTGTCCC TGAGTATGGC TGCGTGGTGA ACTTGCGGAA 4141 GACAGTGGTG AACTTCCCTG TAGAAGACGA GGCCCTGGGT GGCACGGCTT TTGTTCAGAT 4201 GCCGGCCCAC GGCCTATTCC CCTGGTGCGG CCTGCTGCTG GATACCCGGA CCCTGGAGGT 4261 GCAGAGCGAC TACTCCAGCT ATGCCCGGAC CTCCATCAGA GCCAGTCTCA CCTTCAACCG 4321 CGGCTTCAAG GCTGGGAGGA ACATGCGTCG CAAACTCTTT GGGGTCTTGC GGCTGAAGTG 4381 TCACAGCCTG TTTCTGGATT TGCAGGTGAA CAGCCTCCAG ACGGTGTGCA CCAACATCTA 4441 CAAGATCCTC CTGCTGCAGG CGTACAGGTT TCACGCATGT GTGCTGCAGC TCCCATTTCA 4501 TCAGCAAGTT TGGAAGAACC CCACATTTTT CCTGCGCGTC ATCTCTGACA CGGCCTCCCT 4561 CTGCTACTCC ATCCTGAAAG CCAAGAACGC AGGGATGTCG CTGGGGGCCA AGGGCGCCGC 4621 CGGCCCTCTG CCCTCCGAGG CCGTGCAGTG GCTGTGCCAC CAAGCATTCC TGCTCAAGCT 4681 GACTCGACAC CGTGTCACCT ACGTGCCACT CCTGGGGTCA CTCAGGACAG CCCAGACGCA 4741 GCTGAGTCGG AAGCTCCCGG GGACGACGCT GACTGCCCTG GAGGCCGCAG CCAACCCGGC 4801 ACTGCCCTCA GACTTCAAGA CCATCCTGGA CTGAGTCGAC CCTGTGGAAT GTGTGTCAGT 4861 TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA 4921 ATTAGTCAGC AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA 4981 GCATGCATCT CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC 5041 TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG 5101 CAGAGGCCGA GGCCGCCTCG GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG 5161 GAGGCCTAGG CTTTTGCAAA AAGCTTGCCA GCTGGGGCGC CCTCTGGTAA GGTTGGGAAG 5221 CCCTGCAGAT CTGATCCCGG GGGGCAATGA GATATGAAAA AGCCTGAACT CACCGCGACG 5281 TCTGTCGAGA AGTTTCTGAT CGAAAAGTTC GACAGCGTCT CCGACCTGAT GCAGCTCTCG 5341 GAGGGCGAAG AATCTCGTGC TTTCAGCTTC GATGTAGGAG GGCGTGGATA TGTCCTGCGG 5401 GTAAATAGCT GCGCCGATGG TTTCTACAAA GATCGTTATG TTTATCGGCA CTTTGCATCG 5461 GCCGCGCTCC CGATTCCGGA AGTGCTTGAC ATTGGGGAAT TTAGCGAGAG CCTGACCTAT 5521 TGCATCTCCC GCCGTGCACA GGGTGTCACG TTGCAAGACC TGCCTGAAAC CGAACTGCCC 5581 GCTGTTCTGC AGCCGGTCGC GGAGGCCATG GATGCGATCG CTGCGGCCGA TCTTAGCCAG 5641 ACGAGCGGGT TCGGCCCATT CGGACCGCAA GGAATCGGTC AATACACTAC ATGGCGTGAT 5701 TTCATATGCG CGATTGCTGA TCCCCATGTG TATCACTGGC AAACTGTGAT GGACGACACC 5761 GTCAGTGCGT CCGTCGCGCA GGCTCTCGAT GAGCTGATGC TTTGGGCCGA GGACTGCCCC 5821 GAAGTCCGGC ACCTCGTGCA CGCGGATTTC GGCTCCAACA ATGTCCTGAC GGACAATGGC 5881 CGCATAACAG CGGTCATTGA CTGGAGCGAG GCGATGTTCG GGGATTCCCA ATACGAGGTC 5941 GCCAACATCT TCTTCTGGAG GCCGTGGTTG GCTTGTATGG AGCAGCAGAC GCGCTACTTC 6001 GAGCGGAGGC ATCCGGAGCT TGCAGGATCG CCGCGGCTCC GGGCGTATAT GCTCCGCATT 6061 GGTCTTGACC AACTCTATCA GAGCTTGGTT GACGGCAATT TCGATGATGC AGCTTGGGCG 6121 CAGGGTCGAT GCGACGCAAT CGTCCGATCC GGAGCCGGGA CTGTCGGGCG TACACAAATC 6181 GCCCGCAGAA GCGCGGCCGT CTGGACCGAT GGCTGTGTAG AAGTACTCGC CGATAGTGGA 6241 AACCGACGCC CCAGCACTCG TCCGAGGGCA AAGGAATAGA GTAGATGCCG ACCGGGATCT 6301 ATCGATGCGG CGGGTGTGGT GGTTACGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC 6361 GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC 6421 TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTTCTTCTA GTGTAGCCGT AGTTAGGCCA 6481 CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT 6541 GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC 6601 GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG 6661 AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC 6721 CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC 6781 GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT 6841 CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC 6901 CAGCAACGCA TCGATAAAAT AAAAGATTTT ATTTAGTCTC CAGAAAAAGG GGGGAATGAA 6961 AGACCCCACC TGTAGGTTTG GCAAGCTAGC TTAAGTAACG CCATTTTGCA AGGCATGGAA 7021 AAATACATAA CTGAGAATAG AGAAGTTCAG ATCAAGGTCA GGAACAGATG GAACAGCTGA 7081 ATATGGGCCA AACAGGATAT CTGTGGTAAG CAGTTCCTGC CCCGGCTCAG GGCCAAGAAC 7141 AGATGGAACA GCTGAATATG GGCCAAACAG GATATCTGTG GTAAGCAGTT CCTGCCCCGG 7201 CTCAGGGCCA AGAACAGATG GTCCCCAGAT GCGGTCCAGC CCTCAGCAGT TTCTAGAGAA 7261 CCATCAGATG TTTCCAGGGT GCCCCAAGGA CCTGAAATGA CCCTGTGCCT TATTTGAACT 7321 AACCAATCAG TTCGCTTCTC GCTTCTGTTC GCGCGCTTCT GCTCCCCGAG CTCAATAAAA 7381 GAGCCCACAA CCCCTCACTC GGGGCGCCAG TCCTCCGATT GACTGAGTCG CCCGGGTACC 7441 CGTGTATCCA ATAAACCCTC TTGCAGTTGC ATCCGACTTG TGGTCTCGCT GTTCCTTGGG 7501 AGGGTCTCCT CTGAGTGATT GACTACCCGT CAGCGGGGGT CTTTCACATG CAGCATGTAT 7561 CAAAATTAAT TTGGTTTTTT TTCTTAAGTA TTTACATTAA ATGGCCATAG TGCGGCCGCA 7621 AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG 7681 AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG 7741 TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC 7801 GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG 7861 AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG 7921 AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG 7981 GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT 8041 CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC 8101 CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC 8161 ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA 8221 CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC 8281 GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT 8341 CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC 8401 GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA 8461 CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA 8521 TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT 8581 ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA 8641 AAGTGCCACC Tgcagcctga atatgggcca aacaggatat ctgtggtaag cagttcctgc 8701 ccc //
pBABE-hygro-hTERT载体图谱质粒图谱pdf版和pBABE-hygro-hTERT载体序列质粒序列等相关资料下载
pBABE-hygro-hTERT质粒载体应用举例
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